LOCUS       sequence-381586-        8181 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Split TEV assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8181)
  AUTHORS   Wu Y, von Hauff IV, Jensen N, Rossner MJ, Wehr MC
  TITLE     Improved Split TEV GPCR beta-arrestin-2 Recruitment Assays via 
            Systematic Analysis of Signal Peptide and beta-arrestin Binding 
            Motif Variants.
  JOURNAL   Biosensors (Basel). 2022 Dec 29;13(1):48. doi: 10.3390/bios13010048.
  PUBMED    36671883
REFERENCE   2  (bases 1 to 8181)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosensors 
            (Basel)."; date: "2022-12-29"; volume: "13(1):48. doi"; pages: " 
            10.3390/bios13010048"
COMMENT     Sequence Label: pcDNA3_DRD1-NTEV-TCS-GV-2xHA
FEATURES             Location/Qualifiers
     source          1..8181
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    920..944
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2289..2313)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2708..2728
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2735..2764
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2774..3214
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             3257..3277
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3428..3661
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             3665..3691
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3695..3721
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(3734..3752)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3735..3752)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3772..3789)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3778..4002
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4048..4476
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4135..4154)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4345..4366
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4485..4505)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4490..4819
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4670..4805
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4732..4751
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4886..5680
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4940..4959)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5550..5569
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5854..5975
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5891..5910)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5945..5964
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6024..6040)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6024..6040)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6037..6059)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6048..6064
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6072..6078,6079..6096,6097..6102))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6117..6138
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6255..6272)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6426..7014)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6506..6525)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7185..7976,7977..8045))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7808..7827
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8046..8150)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat cccacatcaa caagtttgta caaaaaagca ggctctacca tgaggactct
      961 gaacacctct gccatggacg ggactgggct ggtggtggag agggacttct ctgttcgtat
     1021 cctcactgcc tgtttcctgt cgctgctcat cctgtccacg ctcctgggga acacgctggt
     1081 ctgtgctgcc gttatcaggt tccgacacct gcggtccaag gtgaccaact tctttgtcat
     1141 ctccttggct gtgtcagatc tcttggtggc cgtcctggtc atgccctgga aggcagtggc
     1201 tgagattgct ggcttctggc cctttgggtc cttctgtaac atctgggtgg cctttgacat
     1261 catgtgctcc actgcatcca tcctcaacct ctgtgtgatc agcgtggaca ggtattgggc
     1321 tatctccagc cctttccggt atgagagaaa gatgaccccc aaggcagcct tcatcctgat
     1381 cagtgtggca tggaccttgt ctgtactcat ctccttcatc ccagtgcagc tcagctggca
     1441 caaggcaaaa cccacaagcc cctctgatgg aaatgccact tccctggctg agaccataga
     1501 caactgtgac tccagcctca gcaggacata tgccatctca tcctctgtaa taagctttta
     1561 catccctgtg gccatcatga ttgtcaccta caccaggatc tacaggattg ctcagaaaca
     1621 aatacggcgc attgcggcct tggagagggc agcagtccac gccaagaatt gccagaccac
     1681 cacaggtaat ggaaagcctg tcgaatgttc tcaaccggaa agttctttta agatgtcctt
     1741 caaaagagaa actaaagtcc tgaagactct gtcggtgatc atgggtgtgt ttgtgtgctg
     1801 ttggctacct ttcttcatct tgaactgcat tttgcccttc tgtgggtctg gggagacgca
     1861 gcccttctgc attgattcca acacctttga cgtgtttgtg tggtttgggt gggctaattc
     1921 atccttgaac cccatcattt atgcctttaa tgctgatttt cggaaggcat tttcaaccct
     1981 cttaggatgc tacagacttt gccctgcgac gaataatgcc atagagacgg tgagtatcaa
     2041 taacaatggg gccgcgatgt tttccagcca tcatgagcca cgaggctcca tctccaagga
     2101 gtgcaatctg gtttacctga tcccacatgc tgtgggctcc tctgaggacc tgaaaaagga
     2161 ggaggcagct ggcatcgcca gacccttgga gaagctgtcc ccagccctat cggtcatatt
     2221 ggactatgac actgacgtct ctctggagaa gatccaaccc atcacacaaa acggtcagca
     2281 cccaaccgac ccagctttct tgtacaaagt ggttgatgtc ggaggtggag gtagcggagg
     2341 tggaggtagc ggagagagct tgtttaaggg accacgtgat tacaacccga tatcgagcac
     2401 catttgtcat ttgacgaatg aatctgatgg gcacacaaca tcgttgtatg gtattggatt
     2461 tggtcccttc atcattacaa acaagcactt gtttcgtcgc aataatggaa cactgttggt
     2521 ccaatcacta catggtgtat tcaaggtcaa ggacaccacg actttgcaac aacacctcat
     2581 tgatgggagg gacatgatga ttattcgcat gcctaaggat ttcccaccat ttcctcaaaa
     2641 gctgaaattt agagagccac aaagggaaga gcgcatatgt cttgtgacaa ccaacttcca
     2701 agctcccgag aacctgtact tccagggttc tagagaacag aagctgatct cagaagagga
     2761 tctggcccgg gccatgaagc tactgtcttc tatcgaacaa gcatgcgata tttgccgact
     2821 taaaaagctc aagtgctcca aagaaaaacc gaagtgcgcc aagtgtctga agaacaactg
     2881 ggagtgtcgc tactctccca aaaccaaaag gtctccgctg actagggcac atctgacaga
     2941 agtggaatca aggctagaaa gactggaaca gctatttcta ctgatttttc ctcgagaaga
     3001 ccttgacatg attttgaaaa tggattcttt acaggatata aaagcattgt taacaggatt
     3061 atttgtacaa gataatgtga ataaagatgc cgtcacagat agattggctt cagtggagac
     3121 tgatatgcct ctaacattga gacagcatag aataagtgcg acatcatcat cggaagagag
     3181 tagtaacaaa ggtcaaagac agttgactgt atcgccggaa ttcccgggga tcagcttgat
     3241 aattcgattc accatgccca agaagaagag gaaggtggcg tacagccgcg cgcgtacgaa
     3301 aaacaattac gggtctacca tcgagggcct gctcgatctc ccggacgacg acgcccccga
     3361 agaggcgggg ctggcggctc cgcgcctgtc ctttctcccc gcgggacaca cgcgcagact
     3421 gtcgacggcc cccccgaccg atgtcagcct gggggacgag ctccacttag acggcgagga
     3481 cgtcgcgatg gcgcatgccg acgcgctaga cgatttcgat ctggacatgt tgggggacgg
     3541 ggattccccg ggtccgggat ttacccccca cgactccgcc ccctacggcg ctctggatat
     3601 ggccgacttc gagtttgagc agatgtttac cgatgccctt ggaattgacg agtacggtgg
     3661 gggctaccca tacgatgttc ctgactatgc gggctatccc tatgacgtcc cggactatgc
     3721 atgagggccc tattctatag tgtcacctaa atgctagagc tcgctgatca gcctcgactg
     3781 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     3841 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     3901 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     3961 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     4021 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     4081 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     4141 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     4201 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     4261 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     4321 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     4381 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     4441 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     4501 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     4561 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     4621 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     4681 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4741 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     4801 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     4861 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     4921 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     4981 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     5041 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     5101 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     5161 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     5221 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     5281 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     5341 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     5401 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     5461 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     5521 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     5581 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     5641 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     5701 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     5761 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     5821 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     5881 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     5941 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     6001 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     6061 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     6121 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     6181 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     6241 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     6301 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     6361 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     6421 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     6481 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     6541 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     6601 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     6661 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     6721 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     6781 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     6841 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     6901 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     6961 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     7021 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     7081 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     7141 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     7201 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     7261 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     7321 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     7381 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     7441 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     7501 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     7561 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     7621 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     7681 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     7741 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     7801 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     7861 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     7921 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     7981 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     8041 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     8101 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     8161 cgaaaagtgc cacctgacgt c
//
