LOCUS       sequence-381587-        8241 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Split TEV assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8241)
  AUTHORS   Wu Y, von Hauff IV, Jensen N, Rossner MJ, Wehr MC
  TITLE     Improved Split TEV GPCR beta-arrestin-2 Recruitment Assays via 
            Systematic Analysis of Signal Peptide and beta-arrestin Binding 
            Motif Variants.
  JOURNAL   Biosensors (Basel). 2022 Dec 29;13(1):48. doi: 10.3390/bios13010048.
  PUBMED    36671883
REFERENCE   2  (bases 1 to 8241)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosensors 
            (Basel)."; date: "2022-12-29"; volume: "13(1):48. doi"; pages: " 
            10.3390/bios13010048"
COMMENT     Sequence Label: pcDNA3_SP-DRD2-V2R-NTEV-TCS-GV-2xHA
FEATURES             Location/Qualifiers
     source          1..8241
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    899..923
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2253..2277)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2768..2788
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2795..2824
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2834..3274
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             3317..3337
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3488..3721
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             3725..3751
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3755..3781
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(3794..3812)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3795..3812)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3832..3849)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3838..4062
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4108..4536
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4195..4214)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4405..4426
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4545..4565)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4550..4879
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4730..4865
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4792..4811
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4946..5740
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5000..5019)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5610..5629
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5914..6035
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5951..5970)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6005..6024
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6084..6100)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6084..6100)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6097..6119)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6108..6124
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6132..6138,6139..6156,6157..6162))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6177..6198
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6315..6332)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6486..7074)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6566..6585)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7245..8036,8037..8105))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7868..7887
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8106..8210)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttatcaac
      901 aagtttgtac aaaaaagcag gctccaccat gaagacgatc atcgccctga gctacatctt
      961 ctgcctggta ttcgccgact acaaggacga tgatgacgcc agcatcgata tggatccact
     1021 gaatctgtcc tggtatgatg atgatctgga gaggcagaac tggagccggc ccttcaacgg
     1081 gtcagacggg aaggcggaca gaccccacta caactactat gccacactgc tcaccctgct
     1141 catcgctgtc atcgtcttcg gcaacgtgct ggtgtgcatg gctgtgtcta gagagaaggc
     1201 gctgcagacc accaccaact acctgatcgt cagcctcgca gtggccgacc tcctcgtcgc
     1261 cacactggtc atgccctggg ttgtctacct ggaggtggta ggtgagtgga aattcagcag
     1321 gattcactgt gacatcttcg tcactctgga cgtcatgatg tgcacggcga gcatcctgaa
     1381 cttgtgtgcc atcagcatcg acaggtacac agctgtggcc atgcccatgc tgtacaatac
     1441 gcgctacagc tccaagcgcc gggtcaccgt catgatctcc atcgtctggg tcctgtcctt
     1501 caccatctcc tgcccactcc tcttcggact caataacgca gaccagaacg agtgcatcat
     1561 tgccaacccg gccttcgtgg tctactcctc catcgtctcc ttctacgtgc ccttcattgt
     1621 caccctgctg gtctacatca agatctacat tgtcctccgc agacgccgca agcgagtcaa
     1681 caccaaacgc agcagccgag ctttcagggc ccacctgagg gctccactaa aggaggctgc
     1741 ccggcgagcc caggagctgg agatggagat gctctccagc accagcccac ccgagaggac
     1801 ccggtacagc cccatcccac ccagccacca ccagctgact ctccccgacc cgtcccacca
     1861 cggtctccac agcactcctg acagccccgc caaaccagag aagaatgggc atgccaaaga
     1921 ccaccccaag attgccaaga tctttgagat ccagaccatg cccaatggca aaacccggac
     1981 ctccctcaag accatgagcc gtaggaagct ctcccagcag aaggagaaga aagccactca
     2041 gatgctcgcc attgttctcg gcgtgttcat catctgctgg ctgcccttct tcatcacaca
     2101 catcctgaac atacactgtg actgcaacat cccgcctgtc ctgtacagcg ccttcacgtg
     2161 gctgggctat gtcaacagcg ccgtgaaccc catcatctac accaccttca acattgagtt
     2221 ccgcaaggcc ttcctgaaga tcctccactg cgacccagct ttcttgtaca aagtggttga
     2281 tggtaccgcc cggggacgca ccccacccag cctgggtccc caagatgagt cctgcaccac
     2341 cgccagctcc tccctggcca aggacacttc atcggatgtc ggaggtggag gtagcggagg
     2401 tggaggtagc ggagagagct tgtttaaggg accacgtgat tacaacccga tatcgagcac
     2461 catttgtcat ttgacgaatg aatctgatgg gcacacaaca tcgttgtatg gtattggatt
     2521 tggtcccttc atcattacaa acaagcactt gtttcgtcgc aataatggaa cactgttggt
     2581 ccaatcacta catggtgtat tcaaggtcaa ggacaccacg actttgcaac aacacctcat
     2641 tgatgggagg gacatgatga ttattcgcat gcctaaggat ttcccaccat ttcctcaaaa
     2701 gctgaaattt agagagccac aaagggaaga gcgcatatgt cttgtgacaa ccaacttcca
     2761 agctcccgag aacctgtact tccagggttc tagagaacag aagctgatct cagaagagga
     2821 tctggcccgg gccatgaagc tactgtcttc tatcgaacaa gcatgcgata tttgccgact
     2881 taaaaagctc aagtgctcca aagaaaaacc gaagtgcgcc aagtgtctga agaacaactg
     2941 ggagtgtcgc tactctccca aaaccaaaag gtctccgctg actagggcac atctgacaga
     3001 agtggaatca aggctagaaa gactggaaca gctatttcta ctgatttttc ctcgagaaga
     3061 ccttgacatg attttgaaaa tggattcttt acaggatata aaagcattgt taacaggatt
     3121 atttgtacaa gataatgtga ataaagatgc cgtcacagat agattggctt cagtggagac
     3181 tgatatgcct ctaacattga gacagcatag aataagtgcg acatcatcat cggaagagag
     3241 tagtaacaaa ggtcaaagac agttgactgt atcgccggaa ttcccgggga tcagcttgat
     3301 aattcgattc accatgccca agaagaagag gaaggtggcg tacagccgcg cgcgtacgaa
     3361 aaacaattac gggtctacca tcgagggcct gctcgatctc ccggacgacg acgcccccga
     3421 agaggcgggg ctggcggctc cgcgcctgtc ctttctcccc gcgggacaca cgcgcagact
     3481 gtcgacggcc cccccgaccg atgtcagcct gggggacgag ctccacttag acggcgagga
     3541 cgtcgcgatg gcgcatgccg acgcgctaga cgatttcgat ctggacatgt tgggggacgg
     3601 ggattccccg ggtccgggat ttacccccca cgactccgcc ccctacggcg ctctggatat
     3661 ggccgacttc gagtttgagc agatgtttac cgatgccctt ggaattgacg agtacggtgg
     3721 gggctaccca tacgatgttc ctgactatgc gggctatccc tatgacgtcc cggactatgc
     3781 atgagggccc tattctatag tgtcacctaa atgctagagc tcgctgatca gcctcgactg
     3841 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     3901 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     3961 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     4021 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     4081 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     4141 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     4201 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     4261 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     4321 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     4381 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     4441 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     4501 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     4561 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     4621 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     4681 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     4741 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4801 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     4861 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     4921 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     4981 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     5041 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     5101 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     5161 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     5221 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     5281 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     5341 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     5401 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     5461 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     5521 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     5581 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     5641 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     5701 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     5761 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     5821 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     5881 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     5941 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     6001 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     6061 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     6121 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     6181 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     6241 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     6301 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     6361 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     6421 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     6481 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     6541 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     6601 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     6661 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     6721 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     6781 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     6841 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     6901 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     6961 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     7021 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     7081 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     7141 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     7201 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     7261 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     7321 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     7381 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     7441 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     7501 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     7561 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     7621 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     7681 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     7741 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     7801 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     7861 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     7921 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     7981 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     8041 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     8101 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     8161 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     8221 cgaaaagtgc cacctgacgt c
//
