LOCUS       sequence-381589-        8085 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Split TEV assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8085)
  AUTHORS   Wu Y, von Hauff IV, Jensen N, Rossner MJ, Wehr MC
  TITLE     Improved Split TEV GPCR beta-arrestin-2 Recruitment Assays via 
            Systematic Analysis of Signal Peptide and beta-arrestin Binding 
            Motif Variants.
  JOURNAL   Biosensors (Basel). 2022 Dec 29;13(1):48. doi: 10.3390/bios13010048.
  PUBMED    36671883
REFERENCE   2  (bases 1 to 8085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosensors 
            (Basel)."; date: "2022-12-29"; volume: "13(1):48. doi"; pages: " 
            10.3390/bios13010048"
COMMENT     Sequence Label: pcDNA3_DRD2-NTEV-TCS-GV-2xHA
FEATURES             Location/Qualifiers
     source          1..8085
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    920..944
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2193..2217)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2612..2632
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2639..2668
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2678..3118
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             3161..3181
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3332..3565
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             3569..3595
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3599..3625
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(3638..3656)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3639..3656)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3676..3693)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3682..3906
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3952..4380
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4039..4058)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4249..4270
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4389..4409)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4394..4723
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4574..4709
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4636..4655
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4790..5584
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4844..4863)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5454..5473
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5758..5879
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5795..5814)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5849..5868
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5928..5944)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5928..5944)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5941..5963)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5952..5968
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5976..5982,5983..6000,6001..6006))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6021..6042
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6159..6176)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6330..6918)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6410..6429)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7089..7880,7881..7949))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7712..7731
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7950..8054)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat cccacatcaa caagtttgta caaaaaagca ggctctacca tggatccact
      961 gaatctgtcc tggtatgatg atgatctgga gaggcagaac tggagccggc ccttcaacgg
     1021 gtcagacggg aaggcggaca gaccccacta caactactat gccacactgc tcaccctgct
     1081 catcgctgtc atcgtcttcg gcaacgtgct ggtgtgcatg gctgtgtcta gagagaaggc
     1141 gctgcagacc accaccaact acctgatcgt cagcctcgca gtggccgacc tcctcgtcgc
     1201 cacactggtc atgccctggg ttgtctacct ggaggtggta ggtgagtgga aattcagcag
     1261 gattcactgt gacatcttcg tcactctgga cgtcatgatg tgcacggcga gcatcctgaa
     1321 cttgtgtgcc atcagcatcg acaggtacac agctgtggcc atgcccatgc tgtacaatac
     1381 gcgctacagc tccaagcgcc gggtcaccgt catgatctcc atcgtctggg tcctgtcctt
     1441 caccatctcc tgcccactcc tcttcggact caataacgca gaccagaacg agtgcatcat
     1501 tgccaacccg gccttcgtgg tctactcctc catcgtctcc ttctacgtgc ccttcattgt
     1561 caccctgctg gtctacatca agatctacat tgtcctccgc agacgccgca agcgagtcaa
     1621 caccaaacgc agcagccgag ctttcagggc ccacctgagg gctccactaa aggaggctgc
     1681 ccggcgagcc caggagctgg agatggagat gctctccagc accagcccac ccgagaggac
     1741 ccggtacagc cccatcccac ccagccacca ccagctgact ctccccgacc cgtcccacca
     1801 cggtctccac agcactcctg acagccccgc caaaccagag aagaatgggc atgccaaaga
     1861 ccaccccaag attgccaaga tctttgagat ccagaccatg cccaatggca aaacccggac
     1921 ctccctcaag accatgagcc gtaggaagct ctcccagcag aaggagaaga aagccactca
     1981 gatgctcgcc attgttctcg gcgtgttcat catctgctgg ctgcccttct tcatcacaca
     2041 catcctgaac atacactgtg actgcaacat cccgcctgtc ctgtacagcg ccttcacgtg
     2101 gctgggctat gtcaacagcg ccgtgaaccc catcatctac accaccttca acattgagtt
     2161 ccgcaaggcc ttcctgaaga tcctccactg cgacccagct ttcttgtaca aagtggttga
     2221 tgtcggaggt ggaggtagcg gaggtggagg tagcggagag agcttgttta agggaccacg
     2281 tgattacaac ccgatatcga gcaccatttg tcatttgacg aatgaatctg atgggcacac
     2341 aacatcgttg tatggtattg gatttggtcc cttcatcatt acaaacaagc acttgtttcg
     2401 tcgcaataat ggaacactgt tggtccaatc actacatggt gtattcaagg tcaaggacac
     2461 cacgactttg caacaacacc tcattgatgg gagggacatg atgattattc gcatgcctaa
     2521 ggatttccca ccatttcctc aaaagctgaa atttagagag ccacaaaggg aagagcgcat
     2581 atgtcttgtg acaaccaact tccaagctcc cgagaacctg tacttccagg gttctagaga
     2641 acagaagctg atctcagaag aggatctggc ccgggccatg aagctactgt cttctatcga
     2701 acaagcatgc gatatttgcc gacttaaaaa gctcaagtgc tccaaagaaa aaccgaagtg
     2761 cgccaagtgt ctgaagaaca actgggagtg tcgctactct cccaaaacca aaaggtctcc
     2821 gctgactagg gcacatctga cagaagtgga atcaaggcta gaaagactgg aacagctatt
     2881 tctactgatt tttcctcgag aagaccttga catgattttg aaaatggatt ctttacagga
     2941 tataaaagca ttgttaacag gattatttgt acaagataat gtgaataaag atgccgtcac
     3001 agatagattg gcttcagtgg agactgatat gcctctaaca ttgagacagc atagaataag
     3061 tgcgacatca tcatcggaag agagtagtaa caaaggtcaa agacagttga ctgtatcgcc
     3121 ggaattcccg gggatcagct tgataattcg attcaccatg cccaagaaga agaggaaggt
     3181 ggcgtacagc cgcgcgcgta cgaaaaacaa ttacgggtct accatcgagg gcctgctcga
     3241 tctcccggac gacgacgccc ccgaagaggc ggggctggcg gctccgcgcc tgtcctttct
     3301 ccccgcggga cacacgcgca gactgtcgac ggcccccccg accgatgtca gcctggggga
     3361 cgagctccac ttagacggcg aggacgtcgc gatggcgcat gccgacgcgc tagacgattt
     3421 cgatctggac atgttggggg acggggattc cccgggtccg ggatttaccc cccacgactc
     3481 cgccccctac ggcgctctgg atatggccga cttcgagttt gagcagatgt ttaccgatgc
     3541 ccttggaatt gacgagtacg gtgggggcta cccatacgat gttcctgact atgcgggcta
     3601 tccctatgac gtcccggact atgcatgagg gccctattct atagtgtcac ctaaatgcta
     3661 gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     3721 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     3781 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     3841 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     3901 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
     3961 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     4021 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     4081 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     4141 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     4201 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     4261 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     4321 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     4381 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     4441 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     4501 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     4561 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     4621 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     4681 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     4741 tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     4801 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     4861 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     4921 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
     4981 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     5041 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     5101 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     5161 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     5221 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     5281 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
     5341 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     5401 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     5461 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     5521 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     5581 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     5641 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     5701 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     5761 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     5821 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     5881 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     5941 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     6001 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     6061 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     6121 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     6181 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     6241 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     6301 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     6361 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     6421 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     6481 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     6541 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     6601 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     6661 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     6721 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     6781 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     6841 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     6901 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     6961 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     7021 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     7081 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     7141 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     7201 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     7261 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     7321 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     7381 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     7441 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     7501 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     7561 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     7621 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     7681 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     7741 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     7801 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     7861 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     7921 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     7981 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     8041 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
