LOCUS       sequence-381597-        8160 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Split TEV assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8160)
  AUTHORS   Wu Y, von Hauff IV, Jensen N, Rossner MJ, Wehr MC
  TITLE     Improved Split TEV GPCR beta-arrestin-2 Recruitment Assays via 
            Systematic Analysis of Signal Peptide and beta-arrestin Binding 
            Motif Variants.
  JOURNAL   Biosensors (Basel). 2022 Dec 29;13(1):48. doi: 10.3390/bios13010048.
  PUBMED    36671883
REFERENCE   2  (bases 1 to 8160)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosensors 
            (Basel)."; date: "2022-12-29"; volume: "13(1):48. doi"; pages: " 
            10.3390/bios13010048"
COMMENT     Sequence Label: pcDNA3_DRD2-V2R-NTEV-TCS-GV-2xHA
FEATURES             Location/Qualifiers
     source          1..8160
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    899..923
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2172..2196)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2687..2707
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2714..2743
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2753..3193
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             3236..3256
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3407..3640
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             3644..3670
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3674..3700
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(3713..3731)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3714..3731)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3751..3768)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3757..3981
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4027..4455
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4114..4133)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4324..4345
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4464..4484)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4469..4798
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4649..4784
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4711..4730
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4865..5659
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4919..4938)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5529..5548
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5833..5954
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5870..5889)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5924..5943
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6003..6019)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6003..6019)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6016..6038)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6027..6043
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6051..6057,6058..6075,6076..6081))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6096..6117
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6234..6251)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6405..6993)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6485..6504)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7164..7955,7956..8024))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7787..7806
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8025..8129)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttatcaac
      901 aagtttgtac aaaaaagcag gctctaccat ggatccactg aatctgtcct ggtatgatga
      961 tgatctggag aggcagaact ggagccggcc cttcaacggg tcagacggga aggcggacag
     1021 accccactac aactactatg ccacactgct caccctgctc atcgctgtca tcgtcttcgg
     1081 caacgtgctg gtgtgcatgg ctgtgtctag agagaaggcg ctgcagacca ccaccaacta
     1141 cctgatcgtc agcctcgcag tggccgacct cctcgtcgcc acactggtca tgccctgggt
     1201 tgtctacctg gaggtggtag gtgagtggaa attcagcagg attcactgtg acatcttcgt
     1261 cactctggac gtcatgatgt gcacggcgag catcctgaac ttgtgtgcca tcagcatcga
     1321 caggtacaca gctgtggcca tgcccatgct gtacaatacg cgctacagct ccaagcgccg
     1381 ggtcaccgtc atgatctcca tcgtctgggt cctgtccttc accatctcct gcccactcct
     1441 cttcggactc aataacgcag accagaacga gtgcatcatt gccaacccgg ccttcgtggt
     1501 ctactcctcc atcgtctcct tctacgtgcc cttcattgtc accctgctgg tctacatcaa
     1561 gatctacatt gtcctccgca gacgccgcaa gcgagtcaac accaaacgca gcagccgagc
     1621 tttcagggcc cacctgaggg ctccactaaa ggaggctgcc cggcgagccc aggagctgga
     1681 gatggagatg ctctccagca ccagcccacc cgagaggacc cggtacagcc ccatcccacc
     1741 cagccaccac cagctgactc tccccgaccc gtcccaccac ggtctccaca gcactcctga
     1801 cagccccgcc aaaccagaga agaatgggca tgccaaagac caccccaaga ttgccaagat
     1861 ctttgagatc cagaccatgc ccaatggcaa aacccggacc tccctcaaga ccatgagccg
     1921 taggaagctc tcccagcaga aggagaagaa agccactcag atgctcgcca ttgttctcgg
     1981 cgtgttcatc atctgctggc tgcccttctt catcacacac atcctgaaca tacactgtga
     2041 ctgcaacatc ccgcctgtcc tgtacagcgc cttcacgtgg ctgggctatg tcaacagcgc
     2101 cgtgaacccc atcatctaca ccaccttcaa cattgagttc cgcaaggcct tcctgaagat
     2161 cctccactgc gacccagctt tcttgtacaa agtggttgat ggtaccgccc ggggacgcac
     2221 cccacccagc ctgggtcccc aagatgagtc ctgcaccacc gccagctcct ccctggccaa
     2281 ggacacttca tcggatgtcg gaggtggagg tagcggaggt ggaggtagcg gagagagctt
     2341 gtttaaggga ccacgtgatt acaacccgat atcgagcacc atttgtcatt tgacgaatga
     2401 atctgatggg cacacaacat cgttgtatgg tattggattt ggtcccttca tcattacaaa
     2461 caagcacttg tttcgtcgca ataatggaac actgttggtc caatcactac atggtgtatt
     2521 caaggtcaag gacaccacga ctttgcaaca acacctcatt gatgggaggg acatgatgat
     2581 tattcgcatg cctaaggatt tcccaccatt tcctcaaaag ctgaaattta gagagccaca
     2641 aagggaagag cgcatatgtc ttgtgacaac caacttccaa gctcccgaga acctgtactt
     2701 ccagggttct agagaacaga agctgatctc agaagaggat ctggcccggg ccatgaagct
     2761 actgtcttct atcgaacaag catgcgatat ttgccgactt aaaaagctca agtgctccaa
     2821 agaaaaaccg aagtgcgcca agtgtctgaa gaacaactgg gagtgtcgct actctcccaa
     2881 aaccaaaagg tctccgctga ctagggcaca tctgacagaa gtggaatcaa ggctagaaag
     2941 actggaacag ctatttctac tgatttttcc tcgagaagac cttgacatga ttttgaaaat
     3001 ggattcttta caggatataa aagcattgtt aacaggatta tttgtacaag ataatgtgaa
     3061 taaagatgcc gtcacagata gattggcttc agtggagact gatatgcctc taacattgag
     3121 acagcataga ataagtgcga catcatcatc ggaagagagt agtaacaaag gtcaaagaca
     3181 gttgactgta tcgccggaat tcccggggat cagcttgata attcgattca ccatgcccaa
     3241 gaagaagagg aaggtggcgt acagccgcgc gcgtacgaaa aacaattacg ggtctaccat
     3301 cgagggcctg ctcgatctcc cggacgacga cgcccccgaa gaggcggggc tggcggctcc
     3361 gcgcctgtcc tttctccccg cgggacacac gcgcagactg tcgacggccc ccccgaccga
     3421 tgtcagcctg ggggacgagc tccacttaga cggcgaggac gtcgcgatgg cgcatgccga
     3481 cgcgctagac gatttcgatc tggacatgtt gggggacggg gattccccgg gtccgggatt
     3541 taccccccac gactccgccc cctacggcgc tctggatatg gccgacttcg agtttgagca
     3601 gatgtttacc gatgcccttg gaattgacga gtacggtggg ggctacccat acgatgttcc
     3661 tgactatgcg ggctatccct atgacgtccc ggactatgca tgagggccct attctatagt
     3721 gtcacctaaa tgctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat
     3781 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     3841 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     3901 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     3961 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     4021 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     4081 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     4141 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     4201 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     4261 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     4321 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     4381 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     4441 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     4501 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     4561 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     4621 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     4681 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     4741 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     4801 gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt
     4861 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     4921 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     4981 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     5041 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     5101 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     5161 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     5221 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     5281 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     5341 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
     5401 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     5461 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     5521 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     5581 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     5641 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
     5701 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     5761 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     5821 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     5881 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     5941 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     6001 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     6061 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     6121 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     6181 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     6241 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     6301 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     6361 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     6421 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     6481 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     6541 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     6601 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     6661 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     6721 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     6781 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     6841 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     6901 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     6961 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     7021 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     7081 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     7141 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     7201 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     7261 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     7321 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     7381 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     7441 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     7501 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     7561 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     7621 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     7681 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     7741 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     7801 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     7861 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     7921 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     7981 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     8041 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     8101 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
