LOCUS       sequence-381601-        8355 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Split TEV assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8355)
  AUTHORS   Wu Y, von Hauff IV, Jensen N, Rossner MJ, Wehr MC
  TITLE     Improved Split TEV GPCR beta-arrestin-2 Recruitment Assays via 
            Systematic Analysis of Signal Peptide and beta-arrestin Binding 
            Motif Variants.
  JOURNAL   Biosensors (Basel). 2022 Dec 29;13(1):48. doi: 10.3390/bios13010048.
  PUBMED    36671883
REFERENCE   2  (bases 1 to 8355)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosensors 
            (Basel)."; date: "2022-12-29"; volume: "13(1):48. doi"; pages: " 
            10.3390/bios13010048"
COMMENT     Sequence Label: pcDNA3_SP-GCGR-NTEV-TCS-GV-2xHA
FEATURES             Location/Qualifiers
     source          1..8355
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    920..944
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2463..2487)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2882..2902
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2909..2938
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2948..3388
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             3431..3451
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3602..3835
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             3839..3865
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3869..3895
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(3908..3926)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3909..3926)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3946..3963)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3952..4176
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4222..4650
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4309..4328)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4519..4540
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4659..4679)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4664..4993
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4844..4979
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4906..4925
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5060..5854
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5114..5133)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5724..5743
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6028..6149
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6065..6084)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6119..6138
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6198..6214)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6198..6214)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6211..6233)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6222..6238
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6246..6252,6253..6270,6271..6276))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6291..6312
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6429..6446)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6600..7188)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6680..6699)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7359..8150,8151..8219))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7982..8001
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8220..8324)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat cccacatcaa caagtttgta caaaaaagca ggctccacca tgaagacgat
      961 catcgccctg agctacatct tctgcctggt attcgccgac tacaaggacg atgatgacgc
     1021 cagcatcgat atgcccccct gccagccaca gcgacccctg ctgctgttgc tgctgctgct
     1081 ggcctgccag ccacaggtcc cctccgctca ggtgatggac ttcctgtttg agaagtggaa
     1141 gctctacggt gaccagtgtc accacaacct gagcctgctg ccccctccca cggagctggt
     1201 gtgcaacaga accttcgaca agtattcctg ctggccggac acccccgcca ataccacggc
     1261 caacatctcc tgcccctggt acctgccttg gcaccacaaa gtgcaacacc gcttcgtgtt
     1321 caagagatgc gggcccgacg gtcagtgggt gcgtggaccc cgggggcagc cttggcgtga
     1381 tgcctcccag tgccagatgg atggcgagga gattgaggtc cagaaggagg tggccaagat
     1441 gtacagcagc ttccaggtga tgtacacagt gggctacagc ctgtccctgg gggccctgct
     1501 cctcgccttg gccatcctgg ggggcctcag caagctgcac tgcacccgca atgccatcca
     1561 cgcgaatctg tttgcgtcct tcgtgctgaa agccagctcc gtgctggtca ttgatgggct
     1621 gctcaggacc cgctacagcc agaaaattgg cgacgacctc agtgtcagca cctggctcag
     1681 tgatggagcg gtggctggct gccgtgtggc cgcggtgttc atgcaatatg gcatcgtggc
     1741 caactactgc tggctgctgg tggagggcct gtacctgcac aacctgctgg gcctggccac
     1801 cctccccgag aggagcttct tcagcctcta cctgggcatc ggctggggtg cccccatgct
     1861 gttcgtcgtc ccctgggcag tggtcaagtg tctgttcgag aacgtccagt gctggaccag
     1921 caatgacaac atgggcttct ggtggatcct gcggttcccc gtcttcctgg ccatcctgat
     1981 caacttcttc atcttcgtcc gcatcgttca gctgctcgtg gccaagctgc gggcacggca
     2041 gatgcaccac acagactaca agttccggct ggccaagtcc acgctgaccc tcatccctct
     2101 gctgggcgtc cacgaagtgg tcttcgcctt cgtgacggac gagcacgccc agggcaccct
     2161 gcgctccgcc aagctcttct tcgacctctt cctcagctcc ttccagggcc tgctggtggc
     2221 tgtcctctac tgcttcctca acaaggaggt gcagtcggag ctgcggcggc gttggcaccg
     2281 ctggcgcctg ggcaaagtgc tatgggagga gcggaacacc agcaaccaca gggcctcatc
     2341 ttcgcccggc cacggccctc ccagcaagga gctgcagttt gggaggggtg gtggcagcca
     2401 ggattcatct gcggagaccc ccttggctgg tggcctccct agattggctg agagcccctt
     2461 cgacccagct ttcttgtaca aagtggttga tgtcggaggt ggaggtagcg gaggtggagg
     2521 tagcggagag agcttgttta agggaccacg tgattacaac ccgatatcga gcaccatttg
     2581 tcatttgacg aatgaatctg atgggcacac aacatcgttg tatggtattg gatttggtcc
     2641 cttcatcatt acaaacaagc acttgtttcg tcgcaataat ggaacactgt tggtccaatc
     2701 actacatggt gtattcaagg tcaaggacac cacgactttg caacaacacc tcattgatgg
     2761 gagggacatg atgattattc gcatgcctaa ggatttccca ccatttcctc aaaagctgaa
     2821 atttagagag ccacaaaggg aagagcgcat atgtcttgtg acaaccaact tccaagctcc
     2881 cgagaacctg tacttccagg gttctagaga acagaagctg atctcagaag aggatctggc
     2941 ccgggccatg aagctactgt cttctatcga acaagcatgc gatatttgcc gacttaaaaa
     3001 gctcaagtgc tccaaagaaa aaccgaagtg cgccaagtgt ctgaagaaca actgggagtg
     3061 tcgctactct cccaaaacca aaaggtctcc gctgactagg gcacatctga cagaagtgga
     3121 atcaaggcta gaaagactgg aacagctatt tctactgatt tttcctcgag aagaccttga
     3181 catgattttg aaaatggatt ctttacagga tataaaagca ttgttaacag gattatttgt
     3241 acaagataat gtgaataaag atgccgtcac agatagattg gcttcagtgg agactgatat
     3301 gcctctaaca ttgagacagc atagaataag tgcgacatca tcatcggaag agagtagtaa
     3361 caaaggtcaa agacagttga ctgtatcgcc ggaattcccg gggatcagct tgataattcg
     3421 attcaccatg cccaagaaga agaggaaggt ggcgtacagc cgcgcgcgta cgaaaaacaa
     3481 ttacgggtct accatcgagg gcctgctcga tctcccggac gacgacgccc ccgaagaggc
     3541 ggggctggcg gctccgcgcc tgtcctttct ccccgcggga cacacgcgca gactgtcgac
     3601 ggcccccccg accgatgtca gcctggggga cgagctccac ttagacggcg aggacgtcgc
     3661 gatggcgcat gccgacgcgc tagacgattt cgatctggac atgttggggg acggggattc
     3721 cccgggtccg ggatttaccc cccacgactc cgccccctac ggcgctctgg atatggccga
     3781 cttcgagttt gagcagatgt ttaccgatgc ccttggaatt gacgagtacg gtgggggcta
     3841 cccatacgat gttcctgact atgcgggcta tccctatgac gtcccggact atgcatgagg
     3901 gccctattct atagtgtcac ctaaatgcta gagctcgctg atcagcctcg actgtgcctt
     3961 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     4021 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     4081 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     4141 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     4201 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     4261 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     4321 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     4381 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     4441 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     4501 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     4561 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     4621 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     4681 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     4741 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     4801 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     4861 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     4921 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     4981 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac
     5041 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     5101 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     5161 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     5221 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     5281 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     5341 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     5401 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     5461 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     5521 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     5581 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     5641 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     5701 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     5761 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     5821 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     5881 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     5941 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     6001 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     6061 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     6121 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     6181 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     6241 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     6301 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     6361 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     6421 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     6481 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     6541 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     6601 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     6661 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     6721 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     6781 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     6841 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     6901 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     6961 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     7021 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     7081 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     7141 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     7201 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     7261 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     7321 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     7381 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     7441 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     7501 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     7561 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     7621 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     7681 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     7741 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     7801 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     7861 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     7921 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     7981 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     8041 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     8101 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     8161 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     8221 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     8281 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     8341 gtgccacctg acgtc
//
