LOCUS       sequence-381607-        8166 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Split TEV assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8166)
  AUTHORS   Wu Y, von Hauff IV, Jensen N, Rossner MJ, Wehr MC
  TITLE     Improved Split TEV GPCR beta-arrestin-2 Recruitment Assays via 
            Systematic Analysis of Signal Peptide and beta-arrestin Binding 
            Motif Variants.
  JOURNAL   Biosensors (Basel). 2022 Dec 29;13(1):48. doi: 10.3390/bios13010048.
  PUBMED    36671883
REFERENCE   2  (bases 1 to 8166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosensors 
            (Basel)."; date: "2022-12-29"; volume: "13(1):48. doi"; pages: " 
            10.3390/bios13010048"
COMMENT     Sequence Label: pcDNA3_SP-DRD2-NTEV-TCS-GV-2xHA
FEATURES             Location/Qualifiers
     source          1..8166
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    920..944
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2274..2298)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2693..2713
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2720..2749
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2759..3199
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             3242..3262
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3413..3646
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             3650..3676
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3680..3706
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(3719..3737)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3720..3737)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3757..3774)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3763..3987
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4033..4461
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4120..4139)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4330..4351
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4470..4490)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4475..4804
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4655..4790
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4717..4736
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4871..5665
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4925..4944)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5535..5554
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5839..5960
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5876..5895)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5930..5949
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6009..6025)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6009..6025)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6022..6044)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6033..6049
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6057..6063,6064..6081,6082..6087))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6102..6123
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6240..6257)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6411..6999)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6491..6510)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7170..7961,7962..8030))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7793..7812
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8031..8135)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat cccacatcaa caagtttgta caaaaaagca ggctccacca tgaagacgat
      961 catcgccctg agctacatct tctgcctggt attcgccgac tacaaggacg atgatgacgc
     1021 cagcatcgat atggatccac tgaatctgtc ctggtatgat gatgatctgg agaggcagaa
     1081 ctggagccgg cccttcaacg ggtcagacgg gaaggcggac agaccccact acaactacta
     1141 tgccacactg ctcaccctgc tcatcgctgt catcgtcttc ggcaacgtgc tggtgtgcat
     1201 ggctgtgtct agagagaagg cgctgcagac caccaccaac tacctgatcg tcagcctcgc
     1261 agtggccgac ctcctcgtcg ccacactggt catgccctgg gttgtctacc tggaggtggt
     1321 aggtgagtgg aaattcagca ggattcactg tgacatcttc gtcactctgg acgtcatgat
     1381 gtgcacggcg agcatcctga acttgtgtgc catcagcatc gacaggtaca cagctgtggc
     1441 catgcccatg ctgtacaata cgcgctacag ctccaagcgc cgggtcaccg tcatgatctc
     1501 catcgtctgg gtcctgtcct tcaccatctc ctgcccactc ctcttcggac tcaataacgc
     1561 agaccagaac gagtgcatca ttgccaaccc ggccttcgtg gtctactcct ccatcgtctc
     1621 cttctacgtg cccttcattg tcaccctgct ggtctacatc aagatctaca ttgtcctccg
     1681 cagacgccgc aagcgagtca acaccaaacg cagcagccga gctttcaggg cccacctgag
     1741 ggctccacta aaggaggctg cccggcgagc ccaggagctg gagatggaga tgctctccag
     1801 caccagccca cccgagagga cccggtacag ccccatccca cccagccacc accagctgac
     1861 tctccccgac ccgtcccacc acggtctcca cagcactcct gacagccccg ccaaaccaga
     1921 gaagaatggg catgccaaag accaccccaa gattgccaag atctttgaga tccagaccat
     1981 gcccaatggc aaaacccgga cctccctcaa gaccatgagc cgtaggaagc tctcccagca
     2041 gaaggagaag aaagccactc agatgctcgc cattgttctc ggcgtgttca tcatctgctg
     2101 gctgcccttc ttcatcacac acatcctgaa catacactgt gactgcaaca tcccgcctgt
     2161 cctgtacagc gccttcacgt ggctgggcta tgtcaacagc gccgtgaacc ccatcatcta
     2221 caccaccttc aacattgagt tccgcaaggc cttcctgaag atcctccact gcgacccagc
     2281 tttcttgtac aaagtggttg atgtcggagg tggaggtagc ggaggtggag gtagcggaga
     2341 gagcttgttt aagggaccac gtgattacaa cccgatatcg agcaccattt gtcatttgac
     2401 gaatgaatct gatgggcaca caacatcgtt gtatggtatt ggatttggtc ccttcatcat
     2461 tacaaacaag cacttgtttc gtcgcaataa tggaacactg ttggtccaat cactacatgg
     2521 tgtattcaag gtcaaggaca ccacgacttt gcaacaacac ctcattgatg ggagggacat
     2581 gatgattatt cgcatgccta aggatttccc accatttcct caaaagctga aatttagaga
     2641 gccacaaagg gaagagcgca tatgtcttgt gacaaccaac ttccaagctc ccgagaacct
     2701 gtacttccag ggttctagag aacagaagct gatctcagaa gaggatctgg cccgggccat
     2761 gaagctactg tcttctatcg aacaagcatg cgatatttgc cgacttaaaa agctcaagtg
     2821 ctccaaagaa aaaccgaagt gcgccaagtg tctgaagaac aactgggagt gtcgctactc
     2881 tcccaaaacc aaaaggtctc cgctgactag ggcacatctg acagaagtgg aatcaaggct
     2941 agaaagactg gaacagctat ttctactgat ttttcctcga gaagaccttg acatgatttt
     3001 gaaaatggat tctttacagg atataaaagc attgttaaca ggattatttg tacaagataa
     3061 tgtgaataaa gatgccgtca cagatagatt ggcttcagtg gagactgata tgcctctaac
     3121 attgagacag catagaataa gtgcgacatc atcatcggaa gagagtagta acaaaggtca
     3181 aagacagttg actgtatcgc cggaattccc ggggatcagc ttgataattc gattcaccat
     3241 gcccaagaag aagaggaagg tggcgtacag ccgcgcgcgt acgaaaaaca attacgggtc
     3301 taccatcgag ggcctgctcg atctcccgga cgacgacgcc cccgaagagg cggggctggc
     3361 ggctccgcgc ctgtcctttc tccccgcggg acacacgcgc agactgtcga cggccccccc
     3421 gaccgatgtc agcctggggg acgagctcca cttagacggc gaggacgtcg cgatggcgca
     3481 tgccgacgcg ctagacgatt tcgatctgga catgttgggg gacggggatt ccccgggtcc
     3541 gggatttacc ccccacgact ccgcccccta cggcgctctg gatatggccg acttcgagtt
     3601 tgagcagatg tttaccgatg cccttggaat tgacgagtac ggtgggggct acccatacga
     3661 tgttcctgac tatgcgggct atccctatga cgtcccggac tatgcatgag ggccctattc
     3721 tatagtgtca cctaaatgct agagctcgct gatcagcctc gactgtgcct tctagttgcc
     3781 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     3841 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     3901 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     3961 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     4021 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     4081 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     4141 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     4201 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     4261 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     4321 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     4381 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     4441 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     4501 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     4561 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     4621 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     4681 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     4741 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     4801 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg
     4861 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     4921 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     4981 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     5041 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     5101 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     5161 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     5221 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     5281 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     5341 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     5401 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     5461 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     5521 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     5581 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     5641 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     5701 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     5761 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     5821 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     5881 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     5941 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     6001 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     6061 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     6121 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     6181 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     6241 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     6301 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     6361 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6421 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     6481 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     6541 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     6601 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     6661 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     6721 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     6781 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     6841 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     6901 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     6961 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7021 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7081 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7141 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     7201 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     7261 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     7321 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     7381 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     7441 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     7501 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     7561 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     7621 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     7681 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     7741 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     7801 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     7861 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     7921 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     7981 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     8041 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     8101 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     8161 gacgtc
//
