LOCUS       sequence-381610-        8313 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Split TEV assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8313)
  AUTHORS   Wu Y, von Hauff IV, Jensen N, Rossner MJ, Wehr MC
  TITLE     Improved Split TEV GPCR beta-arrestin-2 Recruitment Assays via 
            Systematic Analysis of Signal Peptide and beta-arrestin Binding 
            Motif Variants.
  JOURNAL   Biosensors (Basel). 2022 Dec 29;13(1):48. doi: 10.3390/bios13010048.
  PUBMED    36671883
REFERENCE   2  (bases 1 to 8313)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosensors 
            (Basel)."; date: "2022-12-29"; volume: "13(1):48. doi"; pages: " 
            10.3390/bios13010048"
COMMENT     Sequence Label: pcDNA3_SP-GLP1R-NTEV-TCS-GV-2xHA
FEATURES             Location/Qualifiers
     source          1..8313
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    920..944
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2421..2445)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2840..2860
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2867..2896
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2906..3346
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             3389..3409
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3560..3793
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             3797..3823
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3827..3853
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(3866..3884)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3867..3884)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3904..3921)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3910..4134
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4180..4608
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4267..4286)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4477..4498
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4617..4637)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4622..4951
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4802..4937
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4864..4883
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5018..5812
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5072..5091)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5682..5701
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5986..6107
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6023..6042)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6077..6096
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6156..6172)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6156..6172)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6169..6191)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6180..6196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6204..6210,6211..6228,6229..6234))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6249..6270
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6387..6404)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6558..7146)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6638..6657)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7317..8108,8109..8177))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7940..7959
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8178..8282)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat cccacatcaa caagtttgta caaaaaagca ggctccacca tgaagacgat
      961 catcgccctg agctacatct tctgcctggt attcgccgac tacaaggacg atgatgacgc
     1021 cagcatcgat atggccggcg cccccggccc gctgcgcctt gcgctgctgc tgctcgggat
     1081 ggtgggcagg gccggccccc gcccccaggg tgccactgtg tccctctggg agacggtgca
     1141 gaaatggcga gaataccgac gccagtgcca gcgctccctg actgaggatc cacctcctgc
     1201 cacagacttg ttctgcaacc ggaccttcga tgaatacgcc tgctggccag atggggagcc
     1261 aggctcgttc gtgaatgtca gctgcccctg gtacctgccc tgggccagca gtgtgccgca
     1321 gggccacgtg taccggttct gcacagctga aggcctctgg ctgcagaagg acaactccag
     1381 cctgccctgg agggacttgt cggagtgcga ggagtccaag cgaggggaaa gaagctcccc
     1441 ggaggagcag ctcctgttcc tctacatcat ctacacggtg ggctacgcac tctccttctc
     1501 tgctctggtt atcgcctctg cgatcctcct cggcttcaga cacctgcact gcaccaggaa
     1561 ctacatccac ctgaacctgt ttgcatcctt catcctgcga gcattgtccg tcttcatcaa
     1621 ggacgcagcc ctgaagtgga tgtatagcac agccgcccag cagcaccagt gggatgggct
     1681 cctctcctac caggactctc tgagctgccg cctggtgttt ctgctcatgc agtactgtgt
     1741 ggcggccaat tactactggc tcttggtgga gggcgtgtac ctgtacacac tgctggcctt
     1801 ctcggtctta tctgagcaat ggatcttcag gctctacgtg agcataggct ggggtgttcc
     1861 cctgctgttt gttgtcccct ggggcattgt caagtacctc tatgaggacg agggctgctg
     1921 gaccaggaac tccaacatga actactggct cattatccgg ctgcccattc tctttgccat
     1981 tggggtgaac ttcctcatct ttgttcgggt catctgcatc gtggtatcca aactgaaggc
     2041 caatctcatg tgcaagacag acatcaaatg cagacttgcc aagtccacgc tgacactcat
     2101 ccccctgctg gggactcatg aggtcatctt tgcctttgtg atggacgagc acgcccgggg
     2161 gaccctgcgc ttcatcaagc tgtttacaga gctctccttc acctccttcc aggggctgat
     2221 ggtggccata ttatactgct ttgtcaacaa tgaggtccag ctggaatttc ggaagagctg
     2281 ggagcgctgg cggcttgagc acttgcacat ccagagggac agcagcatga agcccctcaa
     2341 gtgtcccacc agcagcctga gcagtggagc cacggcgggc agcagcatgt acacagccac
     2401 ttgccaggcc tcctgcagcg acccagcttt cttgtacaaa gtggttgatg tcggaggtgg
     2461 aggtagcgga ggtggaggta gcggagagag cttgtttaag ggaccacgtg attacaaccc
     2521 gatatcgagc accatttgtc atttgacgaa tgaatctgat gggcacacaa catcgttgta
     2581 tggtattgga tttggtccct tcatcattac aaacaagcac ttgtttcgtc gcaataatgg
     2641 aacactgttg gtccaatcac tacatggtgt attcaaggtc aaggacacca cgactttgca
     2701 acaacacctc attgatggga gggacatgat gattattcgc atgcctaagg atttcccacc
     2761 atttcctcaa aagctgaaat ttagagagcc acaaagggaa gagcgcatat gtcttgtgac
     2821 aaccaacttc caagctcccg agaacctgta cttccagggt tctagagaac agaagctgat
     2881 ctcagaagag gatctggccc gggccatgaa gctactgtct tctatcgaac aagcatgcga
     2941 tatttgccga cttaaaaagc tcaagtgctc caaagaaaaa ccgaagtgcg ccaagtgtct
     3001 gaagaacaac tgggagtgtc gctactctcc caaaaccaaa aggtctccgc tgactagggc
     3061 acatctgaca gaagtggaat caaggctaga aagactggaa cagctatttc tactgatttt
     3121 tcctcgagaa gaccttgaca tgattttgaa aatggattct ttacaggata taaaagcatt
     3181 gttaacagga ttatttgtac aagataatgt gaataaagat gccgtcacag atagattggc
     3241 ttcagtggag actgatatgc ctctaacatt gagacagcat agaataagtg cgacatcatc
     3301 atcggaagag agtagtaaca aaggtcaaag acagttgact gtatcgccgg aattcccggg
     3361 gatcagcttg ataattcgat tcaccatgcc caagaagaag aggaaggtgg cgtacagccg
     3421 cgcgcgtacg aaaaacaatt acgggtctac catcgagggc ctgctcgatc tcccggacga
     3481 cgacgccccc gaagaggcgg ggctggcggc tccgcgcctg tcctttctcc ccgcgggaca
     3541 cacgcgcaga ctgtcgacgg cccccccgac cgatgtcagc ctgggggacg agctccactt
     3601 agacggcgag gacgtcgcga tggcgcatgc cgacgcgcta gacgatttcg atctggacat
     3661 gttgggggac ggggattccc cgggtccggg atttaccccc cacgactccg ccccctacgg
     3721 cgctctggat atggccgact tcgagtttga gcagatgttt accgatgccc ttggaattga
     3781 cgagtacggt gggggctacc catacgatgt tcctgactat gcgggctatc cctatgacgt
     3841 cccggactat gcatgagggc cctattctat agtgtcacct aaatgctaga gctcgctgat
     3901 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     3961 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     4021 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     4081 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     4141 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     4201 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     4261 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     4321 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     4381 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     4441 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     4501 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     4561 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
     4621 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     4681 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     4741 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     4801 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     4861 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     4921 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     4981 cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     5041 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     5101 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     5161 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
     5221 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     5281 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     5341 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     5401 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     5461 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     5521 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     5581 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     5641 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     5701 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     5761 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     5821 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     5881 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     5941 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     6001 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     6061 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     6121 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     6181 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     6241 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     6301 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     6361 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     6421 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     6481 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     6541 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     6601 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     6661 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     6721 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     6781 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     6841 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     6901 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     6961 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     7021 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     7081 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     7141 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     7201 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     7261 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     7321 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     7381 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     7441 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     7501 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     7561 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     7621 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     7681 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     7741 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     7801 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     7861 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     7921 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     7981 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     8041 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     8101 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     8161 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     8221 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     8281 cgcacatttc cccgaaaagt gccacctgac gtc
//
