LOCUS       sequence-381611-        8358 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Split TEV assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8358)
  AUTHORS   Wu Y, von Hauff IV, Jensen N, Rossner MJ, Wehr MC
  TITLE     Improved Split TEV GPCR beta-arrestin-2 Recruitment Assays via 
            Systematic Analysis of Signal Peptide and beta-arrestin Binding 
            Motif Variants.
  JOURNAL   Biosensors (Basel). 2022 Dec 29;13(1):48. doi: 10.3390/bios13010048.
  PUBMED    36671883
REFERENCE   2  (bases 1 to 8358)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosensors 
            (Basel)."; date: "2022-12-29"; volume: "13(1):48. doi"; pages: " 
            10.3390/bios13010048"
COMMENT     Sequence Label: pcDNA3_SP-HTR2A-V2R-NTEV-TCS-GV-2xHA
FEATURES             Location/Qualifiers
     source          1..8358
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    899..923
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2370..2394)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2885..2905
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2912..2941
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2951..3391
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             3434..3454
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3605..3838
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             3842..3868
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3872..3898
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(3911..3929)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3912..3929)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3949..3966)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3955..4179
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4225..4653
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4312..4331)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4522..4543
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4662..4682)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4667..4996
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4847..4982
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4909..4928
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5063..5857
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5117..5136)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5727..5746
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6031..6152
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6068..6087)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6122..6141
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6201..6217)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6201..6217)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6214..6236)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6225..6241
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6249..6255,6256..6273,6274..6279))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6294..6315
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6432..6449)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6603..7191)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6683..6702)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7362..8153,8154..8222))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7985..8004
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8223..8327)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttatcaac
      901 aagtttgtac aaaaaagcag gctccaccat gaagacgatc atcgccctga gctacatctt
      961 ctgcctggta ttcgccgact acaaggacga tgatgacgcc agcatcgata tgcaattaaa
     1021 tgatgacacc aggctctaca gtaatgactt taactctgga gaagctaaca cttctgatgc
     1081 atttaactgg acagtcgact ctgaaaatcg aaccaacctt tcctgtgaag ggtgcctctc
     1141 accgtcgtgt ctctccttac ttcatctcca ggaaaaaaac tggtctgctt tactgacagc
     1201 cgtagtgatt attctaacta ttgctggaaa catactcgtc atcatggcag tgtccctaga
     1261 gaaaaagctg cagaatgcca ccaactattt cctgatgtca cttgccatag ctgatatgct
     1321 gctgggtttc cttgtcatgc ccgtgtccat gttaaccatc ctgtatgggt accggtggcc
     1381 tctgccgagc aagctttgtg cagtctggat ttacctggac gtgctcttct ccacggcctc
     1441 catcatgcac ctctgcgcca tctcgctgga tcgctacgtc gccatccaga atcccatcca
     1501 ccacagccgc ttcaactcca gaactaaggc atttctgaaa atcattgctg tttggaccat
     1561 atcagtaggt atatccatgc caataccagt ctttgggcta caggacgatt cgaaggtctt
     1621 taaggagggg agttgcttac tcgccgatga taactttgtc ctgatcggct cttttgtgtc
     1681 atttttcatt cccttaacca tcatggtgat cacctacttt ctaactatca agtcactcca
     1741 gaaagaagct actttgtgtg taagtgatct tggcacacgg gccaaattag cttctttcag
     1801 cttcctccct cagagttctt tgtcttcaga aaagctcttc cagcggtcga tccataggga
     1861 gccagggtcc tacacaggca ggaggactat gcagtccatc agcaatgagc aaaaggcatg
     1921 caaggtgctg ggcatcgtct tcttcctgtt tgtggtgatg tggtgccctt tcttcatcac
     1981 aaacatcatg gccgtcatct gcaaagagtc ctgcaatgag gatgtcattg gggccctgct
     2041 caatgtgttt gtttggatcg gttatctctc ttcagcagtc aacccactag tctacacact
     2101 gttcaacaag acctataggt cagccttttc acggtatatt cagtgtcagt acaaggaaaa
     2161 caaaaaacca ttgcagttaa ttttagtgaa cacaataccg gctttggcct acaagtctag
     2221 ccaacttcaa atgggacaaa aaaagaattc aaagcaagat gccaagacaa cagataatga
     2281 ctgctcaatg gttgctctag gaaagcagca ttctgaagag gcttctaaag acaatagcga
     2341 cggagtgaat gaaaaggtga gctgtgtgga cccagctttc ttgtacaaag tggttgatgg
     2401 taccgcccgg ggacgcaccc cacccagcct gggtccccaa gatgagtcct gcaccaccgc
     2461 cagctcctcc ctggccaagg acacttcatc ggatgtcgga ggtggaggta gcggaggtgg
     2521 aggtagcgga gagagcttgt ttaagggacc acgtgattac aacccgatat cgagcaccat
     2581 ttgtcatttg acgaatgaat ctgatgggca cacaacatcg ttgtatggta ttggatttgg
     2641 tcccttcatc attacaaaca agcacttgtt tcgtcgcaat aatggaacac tgttggtcca
     2701 atcactacat ggtgtattca aggtcaagga caccacgact ttgcaacaac acctcattga
     2761 tgggagggac atgatgatta ttcgcatgcc taaggatttc ccaccatttc ctcaaaagct
     2821 gaaatttaga gagccacaaa gggaagagcg catatgtctt gtgacaacca acttccaagc
     2881 tcccgagaac ctgtacttcc agggttctag agaacagaag ctgatctcag aagaggatct
     2941 ggcccgggcc atgaagctac tgtcttctat cgaacaagca tgcgatattt gccgacttaa
     3001 aaagctcaag tgctccaaag aaaaaccgaa gtgcgccaag tgtctgaaga acaactggga
     3061 gtgtcgctac tctcccaaaa ccaaaaggtc tccgctgact agggcacatc tgacagaagt
     3121 ggaatcaagg ctagaaagac tggaacagct atttctactg atttttcctc gagaagacct
     3181 tgacatgatt ttgaaaatgg attctttaca ggatataaaa gcattgttaa caggattatt
     3241 tgtacaagat aatgtgaata aagatgccgt cacagataga ttggcttcag tggagactga
     3301 tatgcctcta acattgagac agcatagaat aagtgcgaca tcatcatcgg aagagagtag
     3361 taacaaaggt caaagacagt tgactgtatc gccggaattc ccggggatca gcttgataat
     3421 tcgattcacc atgcccaaga agaagaggaa ggtggcgtac agccgcgcgc gtacgaaaaa
     3481 caattacggg tctaccatcg agggcctgct cgatctcccg gacgacgacg cccccgaaga
     3541 ggcggggctg gcggctccgc gcctgtcctt tctccccgcg ggacacacgc gcagactgtc
     3601 gacggccccc ccgaccgatg tcagcctggg ggacgagctc cacttagacg gcgaggacgt
     3661 cgcgatggcg catgccgacg cgctagacga tttcgatctg gacatgttgg gggacgggga
     3721 ttccccgggt ccgggattta ccccccacga ctccgccccc tacggcgctc tggatatggc
     3781 cgacttcgag tttgagcaga tgtttaccga tgcccttgga attgacgagt acggtggggg
     3841 ctacccatac gatgttcctg actatgcggg ctatccctat gacgtcccgg actatgcatg
     3901 agggccctat tctatagtgt cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc
     3961 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     4021 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     4081 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     4141 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     4201 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     4261 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     4321 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     4381 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     4441 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     4501 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     4561 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     4621 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     4681 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     4741 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     4801 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     4861 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     4921 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     4981 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga
     5041 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     5101 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     5161 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     5221 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
     5281 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     5341 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     5401 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     5461 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     5521 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     5581 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     5641 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     5701 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     5761 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     5821 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     5881 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     5941 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     6001 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     6061 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     6121 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     6181 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     6241 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     6301 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     6361 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     6421 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     6481 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     6541 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     6601 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     6661 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     6721 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     6781 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     6841 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     6901 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     6961 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     7021 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     7081 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     7141 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     7201 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     7261 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     7321 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     7381 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     7441 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     7501 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     7561 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     7621 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     7681 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     7741 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     7801 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     7861 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     7921 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     7981 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     8041 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     8101 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     8161 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     8221 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     8281 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     8341 aaagtgccac ctgacgtc
//
