LOCUS       sequence-381614-        8331 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Split TEV assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8331)
  AUTHORS   Wu Y, von Hauff IV, Jensen N, Rossner MJ, Wehr MC
  TITLE     Improved Split TEV GPCR beta-arrestin-2 Recruitment Assays via 
            Systematic Analysis of Signal Peptide and beta-arrestin Binding 
            Motif Variants.
  JOURNAL   Biosensors (Basel). 2022 Dec 29;13(1):48. doi: 10.3390/bios13010048.
  PUBMED    36671883
REFERENCE   2  (bases 1 to 8331)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosensors 
            (Basel)."; date: "2022-12-29"; volume: "13(1):48. doi"; pages: " 
            10.3390/bios13010048"
COMMENT     Sequence Label: pcDNA3_HTR2A-V2R-NTEV-TCS-GV-2xHA
FEATURES             Location/Qualifiers
     source          1..8331
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    899..923
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2343..2367)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2858..2878
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2885..2914
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2924..3364
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             3407..3427
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3578..3811
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             3815..3841
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3845..3871
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(3884..3902)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3885..3902)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3922..3939)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3928..4152
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4198..4626
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4285..4304)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4495..4516
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4635..4655)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4640..4969
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4820..4955
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4882..4901
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5036..5830
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5090..5109)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5700..5719
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6004..6125
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6041..6060)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6095..6114
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6174..6190)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6174..6190)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6187..6209)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6198..6214
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6222..6228,6229..6246,6247..6252))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6267..6288
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6405..6422)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6576..7164)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6656..6675)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7335..8126,8127..8195))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7958..7977
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8196..8300)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttatcaac
      901 aagtttgtac aaaaaagcag gctctaccat ggatattctt tgtgaagaaa atacttcttt
      961 gagctcaact acgaactccc taatgcaatt aaatgatgac accaggctct acagtaatga
     1021 ctttaactct ggagaagcta acacttctga tgcatttaac tggacagtcg actctgaaaa
     1081 tcgaaccaac ctttcctgtg aagggtgcct ctcaccgtcg tgtctctcct tacttcatct
     1141 ccaggaaaaa aactggtctg ctttactgac agccgtagtg attattctaa ctattgctgg
     1201 aaacatactc gtcatcatgg cagtgtccct agagaaaaag ctgcagaatg ccaccaacta
     1261 tttcctgatg tcacttgcca tagctgatat gctgctgggt ttccttgtca tgcccgtgtc
     1321 catgttaacc atcctgtatg ggtaccggtg gcctctgccg agcaagcttt gtgcagtctg
     1381 gatttacctg gacgtgctct tctccacggc ctccatcatg cacctctgcg ccatctcgct
     1441 ggatcgctac gtcgccatcc agaatcccat ccaccacagc cgcttcaact ccagaactaa
     1501 ggcatttctg aaaatcattg ctgtttggac catatcagta ggtatatcca tgccaatacc
     1561 agtctttggg ctacaggacg attcgaaggt ctttaaggag gggagttgct tactcgccga
     1621 tgataacttt gtcctgatcg gctcttttgt gtcatttttc attcccttaa ccatcatggt
     1681 gatcacctac tttctaacta tcaagtcact ccagaaagaa gctactttgt gtgtaagtga
     1741 tcttggcaca cgggccaaat tagcttcttt cagcttcctc cctcagagtt ctttgtcttc
     1801 agaaaagctc ttccagcggt cgatccatag ggagccaggg tcctacacag gcaggaggac
     1861 tatgcagtcc atcagcaatg agcaaaaggc atgcaaggtg ctgggcatcg tcttcttcct
     1921 gtttgtggtg atgtggtgcc ctttcttcat cacaaacatc atggccgtca tctgcaaaga
     1981 gtcctgcaat gaggatgtca ttggggccct gctcaatgtg tttgtttgga tcggttatct
     2041 ctcttcagca gtcaacccac tagtctacac actgttcaac aagacctata ggtcagcctt
     2101 ttcacggtat attcagtgtc agtacaagga aaacaaaaaa ccattgcagt taattttagt
     2161 gaacacaata ccggctttgg cctacaagtc tagccaactt caaatgggac aaaaaaagaa
     2221 ttcaaagcaa gatgccaaga caacagataa tgactgctca atggttgctc taggaaagca
     2281 gcattctgaa gaggcttcta aagacaatag cgacggagtg aatgaaaagg tgagctgtgt
     2341 ggacccagct ttcttgtaca aagtggttga tggtaccgcc cggggacgca ccccacccag
     2401 cctgggtccc caagatgagt cctgcaccac cgccagctcc tccctggcca aggacacttc
     2461 atcggatgtc ggaggtggag gtagcggagg tggaggtagc ggagagagct tgtttaaggg
     2521 accacgtgat tacaacccga tatcgagcac catttgtcat ttgacgaatg aatctgatgg
     2581 gcacacaaca tcgttgtatg gtattggatt tggtcccttc atcattacaa acaagcactt
     2641 gtttcgtcgc aataatggaa cactgttggt ccaatcacta catggtgtat tcaaggtcaa
     2701 ggacaccacg actttgcaac aacacctcat tgatgggagg gacatgatga ttattcgcat
     2761 gcctaaggat ttcccaccat ttcctcaaaa gctgaaattt agagagccac aaagggaaga
     2821 gcgcatatgt cttgtgacaa ccaacttcca agctcccgag aacctgtact tccagggttc
     2881 tagagaacag aagctgatct cagaagagga tctggcccgg gccatgaagc tactgtcttc
     2941 tatcgaacaa gcatgcgata tttgccgact taaaaagctc aagtgctcca aagaaaaacc
     3001 gaagtgcgcc aagtgtctga agaacaactg ggagtgtcgc tactctccca aaaccaaaag
     3061 gtctccgctg actagggcac atctgacaga agtggaatca aggctagaaa gactggaaca
     3121 gctatttcta ctgatttttc ctcgagaaga ccttgacatg attttgaaaa tggattcttt
     3181 acaggatata aaagcattgt taacaggatt atttgtacaa gataatgtga ataaagatgc
     3241 cgtcacagat agattggctt cagtggagac tgatatgcct ctaacattga gacagcatag
     3301 aataagtgcg acatcatcat cggaagagag tagtaacaaa ggtcaaagac agttgactgt
     3361 atcgccggaa ttcccgggga tcagcttgat aattcgattc accatgccca agaagaagag
     3421 gaaggtggcg tacagccgcg cgcgtacgaa aaacaattac gggtctacca tcgagggcct
     3481 gctcgatctc ccggacgacg acgcccccga agaggcgggg ctggcggctc cgcgcctgtc
     3541 ctttctcccc gcgggacaca cgcgcagact gtcgacggcc cccccgaccg atgtcagcct
     3601 gggggacgag ctccacttag acggcgagga cgtcgcgatg gcgcatgccg acgcgctaga
     3661 cgatttcgat ctggacatgt tgggggacgg ggattccccg ggtccgggat ttacccccca
     3721 cgactccgcc ccctacggcg ctctggatat ggccgacttc gagtttgagc agatgtttac
     3781 cgatgccctt ggaattgacg agtacggtgg gggctaccca tacgatgttc ctgactatgc
     3841 gggctatccc tatgacgtcc cggactatgc atgagggccc tattctatag tgtcacctaa
     3901 atgctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     3961 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     4021 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     4081 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     4141 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     4201 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     4261 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     4321 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     4381 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     4441 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     4501 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     4561 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     4621 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     4681 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     4741 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     4801 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     4861 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     4921 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     4981 agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat
     5041 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     5101 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     5161 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     5221 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     5281 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     5341 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     5401 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     5461 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     5521 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     5581 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     5641 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     5701 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     5761 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     5821 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     5881 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     5941 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     6001 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     6061 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     6121 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     6181 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     6241 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     6301 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     6361 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     6421 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     6481 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     6541 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     6601 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     6661 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     6721 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     6781 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     6841 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     6901 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     6961 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     7021 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7081 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7141 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7201 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     7261 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     7321 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     7381 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     7441 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     7501 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     7561 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     7621 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     7681 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     7741 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     7801 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     7861 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     7921 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     7981 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     8041 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     8101 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     8161 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     8221 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     8281 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
//
