LOCUS       sequence-381631-        9191 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expressing SARS-CoV-2 BQ.1.1 spike protein for pseudovirus 
            production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9191)
  AUTHORS   Zhou P, Song G, Liu H, Yuan M, He WT, Beutler N, Zhu X, Tse LV, 
            Martinez DR, Schafer A, Anzanello F, Yong P, Peng L, Dueker K, 
            Musharrafieh R, Callaghan S, Capozzola T, Limbo O, Parren M, Garcia 
            E, Rawlings SA, Smith DM, Nemazee D, Jardine JG, Safonova Y, Briney 
            B, Rogers TF, Wilson IA, Baric RS, Gralinski LE, Burton DR, Andrabi 
            R
  TITLE     Broadly neutralizing anti-S2 antibodies protect against all three 
            human betacoronaviruses that cause deadly disease.
  JOURNAL   Immunity. 2023 Mar 14;56(3):669-686.e7. doi: 
            10.1016/j.immuni.2023.02.005. Epub 2023 Feb 16.
  PUBMED    36889306
REFERENCE   2  (bases 1 to 9191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.immuni.2023.02.005"; journalName: "Immunity"; date: 
            "2023-03-14- 14"; volume: "56"; issue: "3"; pages: "669-686.e7"
COMMENT     Sequence Label: pcDNA3.3_SARS2_BQ.1.1
FEATURES             Location/Qualifiers
     source          1..9191
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        50..429
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        430..633
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     584..604
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     630..654
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(4577..4596)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4621..4669
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4871..5299
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4958..4977)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5168..5189
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5308..5328)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5313..5642
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5493..5628
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5555..5574
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5709..6503
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5763..5782)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6373..6392
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6679..6800
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6716..6735)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6770..6789
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6849..6865)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6849..6865)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6862..6884)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6873..6889
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6897..6903,6904..6921,6922..6927))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6942..6963
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7080..7097)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7251..7839)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7331..7350)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8010..8801,8802..8870))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8633..8652
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8871..8975)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9050..9069)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
      721 actgagctcc ttaaggttaa ctctagagaa ttcggatcca tgtttgtgtt cctggtgctg
      781 ctgccactgg tgtccagcca gtgtgtgaac ctgatcacca ggacccaatc ctacaccaac
      841 tccttcacca ggggagtcta ctaccctgac aaggtgttca ggtcctctgt gctgcacagc
      901 acccaggacc tgttcctgcc attcttcagc aatgtgacct ggttccatgc catctctggc
      961 accaatggca ccaagaggtt tgacaaccct gtgctgccat tcaatgatgg agtctacttt
     1021 gccagcacag agaagagcaa catcatcagg ggctggattt ttggcaccac cctggacagc
     1081 aagacccagt ccctgctgat tgtgaacaat gccaccaatg tggtgattaa ggtgtgtgag
     1141 ttccagttct gtaatgaccc attcctggac gtctactacc acaagaacaa caagtcctgg
     1201 atggagtctg agttcagggt ctactcctct gccaacaact gtacctttga atatgtgagc
     1261 caaccattcc tgatggactt ggagggcaag cagggcaact tcaagaacct gagggagttt
     1321 gtgttcaaga acattgatgg ctacttcaag atttacagca aacacacacc aatcaacctg
     1381 gggagggacc tgccacaggg cttctctgcc ttggaaccac tggtggacct gccaattggc
     1441 atcaacatca ccaggttcca gaccctgctg gctctgcaca ggtcctacct gacacctgga
     1501 gactcctcct ctggctggac agcaggagca gcagcctact atgtgggcta cctccaacca
     1561 aggaccttcc tgctgaaata caatgagaat ggcaccatca cagatgctgt ggactgtgcc
     1621 ctggacccac tgtctgagac caagtgtacc ctgaaatcct tcacagtgga gaagggcatc
     1681 taccagacca gcaacttcag ggtccaacca acagagagca ttgtgaggtt tccaaacatc
     1741 accaacctgt gtccatttga cgaggtgttc aatgccacca cgtttgcctc tgtctatgcc
     1801 tggaacagga agaggattag caactgtgtg gctgactact ctgtgctcta caactttgcc
     1861 cccttcttcg ccttcaagtg ttatggagtg agcccaacca aactgaatga cctgtgtttc
     1921 accaatgtct atgctgactc ctttgtgatt aggggaaatg aggtgagtca gattgcccct
     1981 ggacaaacag gcaacattgc tgactacaac tacaaactgc ctgatgactt cacaggctgt
     2041 gtgattgcct ggaacagcaa caagctggac agcacggtgg gaggcaacta caactaccgc
     2101 tacagactgt tcaggaagag caagctgaaa ccatttgaga gggacatcag cacagagatt
     2161 taccaggctg gcaacaaacc atgtaatgga gtggcgggcg tcaactgtta ctttccactc
     2221 cagtcctatg gcttcagacc aacctatgga gtgggccacc aaccatacag ggtggtggtg
     2281 ctgtcctttg aactgctcca tgcccctgcc acagtgtgtg gaccaaagaa gagcaccaac
     2341 ctggtgaaga acaagtgtgt gaacttcaac ttcaatggac tgacaggcac aggagtgctg
     2401 acagagagca acaagaagtt cctgccattc caacagtttg gcagggacat tgctgacacc
     2461 acagatgctg tgagggaccc acagaccttg gagattctgg acatcacacc atgttccttt
     2521 ggaggagtgt ctgtgattac acctggcacc aacaccagca accaggtggc tgtgctctac
     2581 cagggtgtga actgtactga ggtgcctgtg gctatccatg ctgaccaact tacaccaacc
     2641 tggagggtct acagcacagg cagcaatgtg ttccagacca gggctggctg tctgattgga
     2701 gcagagtatg tgaacaactc ctatgagtgt gacatcccaa ttggagcagg catctgtgcc
     2761 tcctaccaga cccagaccaa gagccatagg agggcaaggt ctgtggcaag ccagagcatc
     2821 attgcctaca caatgagtct gggagcagag aactctgtgg cttacagcaa caacagcatt
     2881 gccatcccaa ccaacttcac catctctgtg accacagaaa ttctgcctgt gagtatgacc
     2941 aagacctctg tggactgtac aatgtatatc tgtggagaca gcacagagtg tagcaacctg
     3001 ctgctccaat atggctcctt ctgtacccaa cttaagaggg ctctgacagg cattgctgtg
     3061 gaacaggaca agaacaccca ggaggtgttt gcccaggtga agcagattta caagacacct
     3121 ccaatcaagt actttggagg cttcaacttc agccagattc tgcctgaccc aagcaagcca
     3181 agcaagaggt ccttcattga ggacctgctg ttcaacaagg tgaccctggc tgatgctggc
     3241 ttcatcaagc aatatggaga ctgtctggga gacattgctg caagggacct gatttgtgcc
     3301 cagaagttca atggactgac cgtgctgcct ccactgctga cagatgagat gattgcccaa
     3361 tacacctctg ccctgctggc tggcaccatc acctctggct ggacctttgg agcaggagca
     3421 gccctccaaa tcccatttgc tatgcagatg gcttacaggt tcaatggcat tggagtgacc
     3481 cagaatgtgc tctatgagaa ccagaaactg attgccaacc agttcaactc tgccattggc
     3541 aagattcagg actccctgtc cagcacagcc tctgccctgg gcaaactcca agatgtggtg
     3601 aaccataatg cccaggctct gaacaccctg gtgaagcaac tttccagcaa gtttggagcc
     3661 atctcctctg tgctgaatga catcctgagc agactggaca aggtggaggc tgaggtccag
     3721 attgacagac tgattacagg cagactccaa tccctccaaa cctatgtgac ccaacaactt
     3781 atcagggctg ctgagattag ggcatctgcc aacctggctg ccaccaagat gagtgagtgt
     3841 gtgctgggac aaagcaagag ggtggacttc tgtggcaagg gctaccacct gatgagtttt
     3901 ccacagtctg cccctcatgg agtggtgttc ctgcatgtga cctatgtgcc tgcccaggag
     3961 aagaacttca ccacagcccc tgccatctgc catgatggca aggctcactt tccaagggag
     4021 ggagtgtttg tgagcaatgg cacccactgg tttgtgaccc agaggaactt ctatgaacca
     4081 cagattatca ccacagacaa cacctttgtg tctggcaact gtgatgtggt gattggcatt
     4141 gtgaacaaca cagtctatga cccactccaa cctgaactgg actccttcaa ggaggaactg
     4201 gacaaatact tcaagaacca caccagccct gatgtggacc tgggagacat ctctggcatc
     4261 aatgcctctg tggtgaacat ccagaaggag attgacagac tgaatgaggt ggctaagaac
     4321 ctgaatgagt ccctgattga cctccaagaa ctgggcaaat atgaacaata catcaagtgg
     4381 ccatggtaca tctggctggg cttcattgct ggactgattg ccattgtgat ggtgaccata
     4441 atgctgtgtt gtatgacctc ctgttgttcc tgtctgaaag gctgttgttc ctgtggctcc
     4501 tgttgtaagt gactcgagcc atggaagctt gatatctaac tgactgaacc ggtggtacca
     4561 aacgggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga
     4621 cggcaataaa aagacagaat aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg
     4681 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
     4741 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
     4801 gcagccaacg tcggggcggc aggccctgcc atagcagatc tgcgcagctg gggctctagg
     4861 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     4921 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     4981 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     5041 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     5101 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     5161 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     5221 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     5281 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     5341 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     5401 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     5461 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     5521 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     5581 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     5641 aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat
     5701 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     5761 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     5821 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     5881 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     5941 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     6001 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     6061 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     6121 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     6181 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     6241 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     6301 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     6361 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     6421 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     6481 gccttcttga cgagttcttc tgagcgggac tctggggttc gcgaaatgac cgaccaagcg
     6541 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     6601 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     6661 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     6721 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     6781 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     6841 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     6901 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     6961 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     7021 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     7081 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     7141 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     7201 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     7261 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7321 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7381 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7441 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7501 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7561 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7621 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7681 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7741 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7801 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7861 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7921 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7981 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     8041 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     8101 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     8161 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     8221 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     8281 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     8341 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     8401 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8461 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     8521 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     8581 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     8641 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     8701 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     8761 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     8821 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     8881 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     8941 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     9001 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
     9061 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     9121 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     9181 atctgcttag g
//
