LOCUS       sequence-381634-        9194 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expressing SARS-CoV-2 XBB spike protein for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9194)
  AUTHORS   Zhou P, Song G, Liu H, Yuan M, He WT, Beutler N, Zhu X, Tse LV, 
            Martinez DR, Schafer A, Anzanello F, Yong P, Peng L, Dueker K, 
            Musharrafieh R, Callaghan S, Capozzola T, Limbo O, Parren M, Garcia 
            E, Rawlings SA, Smith DM, Nemazee D, Jardine JG, Safonova Y, Briney 
            B, Rogers TF, Wilson IA, Baric RS, Gralinski LE, Burton DR, Andrabi 
            R
  TITLE     Broadly neutralizing anti-S2 antibodies protect against all three 
            human betacoronaviruses that cause deadly disease.
  JOURNAL   Immunity. 2023 Mar 14;56(3):669-686.e7. doi: 
            10.1016/j.immuni.2023.02.005. Epub 2023 Feb 16.
  PUBMED    36889306
REFERENCE   2  (bases 1 to 9194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.immuni.2023.02.005"; journalName: "Immunity"; date: 
            "2023-03-14- 14"; volume: "56"; issue: "3"; pages: "669-686.e7"
COMMENT     Sequence Label: pcDNA3.3_SARS2_XBB
FEATURES             Location/Qualifiers
     source          1..9194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        8..387
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        388..591
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     542..562
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     588..612
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(4538..4557)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4582..4630
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4832..5260
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4919..4938)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5129..5150
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5269..5289)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5274..5603
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5454..5589
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5516..5535
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5670..6464
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5724..5743)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6334..6353
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6640..6761
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6677..6696)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6731..6750
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6810..6826)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6810..6826)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6823..6845)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6834..6850
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6858..6864,6865..6882,6883..6888))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6903..6924
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7041..7058)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7212..7800)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7292..7311)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7971..8762,8763..8831))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8594..8613
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8832..8936)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9011..9030)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
       61 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      121 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      181 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      241 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      301 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      361 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
      421 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      481 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      541 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
      601 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
      661 gaccgatcca gcctccggac tgagctcctt aaggttaact ctagagaatt cggatccatg
      721 tttgtgttcc tggtgctgct gccactggtg tccagccagt gtgtgaacct gatcaccagg
      781 acccaatcct acaccaactc cttcaccagg ggagtctact accctgacaa ggtgttcagg
      841 tcctctgtgc tgcacagcac ccaggacctg ttcctgccat tcttcagcaa tgtgacctgg
      901 ttccatgcca tccatgtgtc tggcaccaat ggcaccaaga ggtttgacaa ccctgcgctg
      961 ccattcaatg atggagtcta ctttgccagc acagagaaga gcaacatcat caggggctgg
     1021 atttttggca ccaccctgga cagcaagacc cagtccctgc tgattgtgaa caatgccacc
     1081 aatgtggtga ttaaggtgtg tgagttccag ttctgtaatg acccattcct ggacgtctac
     1141 cagaagaaca acaagtcctg gatggagtct gagttcaggg tctactcctc tgccaacaac
     1201 tgtacctttg aatatgtgag ccaaccattc ctgatggact tggagggcaa ggagggcaac
     1261 ttcaagaacc tgagggagtt tgtgttcaag aacattgatg gctacttcaa gatttacagc
     1321 aaacacacac caatcaacct ggagagggac ctgccacagg gcttctctgc cttggaacca
     1381 ctggtggacc tgccaattgg catcaacatc accaggttcc agaccctgct ggctctgcac
     1441 aggtcctacc tgacacctgg agactcctcc tctggctgga cagcaggagc agcagcctac
     1501 tatgtgggct acctccaacc aaggaccttc ctgctgaaat acaatgagaa tggcaccatc
     1561 acagatgctg tggactgtgc cctggaccca ctgtctgaga ccaagtgtac cctgaaatcc
     1621 ttcacagtgg agaagggcat ctaccagacc agcaacttca gggtccaacc aacagagagc
     1681 attgtgaggt ttccaaacat caccaacctg tgtccatttc acgaggtgtt caatgccacc
     1741 acgtttgcct ctgtctatgc ctggaacagg aagaggatta gcaactgtgt ggctgactac
     1801 tctgtgatct acaactttgc ccccttcttc gccttcaagt gttatggagt gagcccaacc
     1861 aaactgaatg acctgtgttt caccaatgtc tatgctgact cctttgtgat taggggaaat
     1921 gaggtgagtc agattgcccc tggacaaaca ggcaacattg ctgactacaa ctacaaactg
     1981 cctgatgact tcacaggctg tgtgattgcc tggaacagca acaagctgga cagcaagccg
     2041 tcaggcaact acaactacct ctacagactg ttcaggaaga gcaagctgaa accatttgag
     2101 agggacatca gcacagagat ttaccaggct ggcaacaaac catgtaatgg agtggcgggc
     2161 tcgaactgtt actctccact ccagtcctat ggcttcagac caacctatgg agtgggccac
     2221 caaccataca gggtggtggt gctgtccttt gaactgctcc atgcccctgc cacagtgtgt
     2281 ggaccaaaga agagcaccaa cctggtgaag aacaagtgtg tgaacttcaa cttcaatgga
     2341 ctgacaggca caggagtgct gacagagagc aacaagaagt tcctgccatt ccaacagttt
     2401 ggcagggaca ttgctgacac cacagatgct gtgagggacc cacagacctt ggagattctg
     2461 gacatcacac catgttcctt tggaggagtg tctgtgatta cacctggcac caacaccagc
     2521 aaccaggtgg ctgtgctcta ccagggtgtg aactgtactg aggtgcctgt ggctatccat
     2581 gctgaccaac ttacaccaac ctggagggtc tacagcacag gcagcaatgt gttccagacc
     2641 agggctggct gtctgattgg agcagagtat gtgaacaact cctatgagtg tgacatccca
     2701 attggagcag gcatctgtgc ctcctaccag acccagacca agagccatag gagggcaagg
     2761 tctgtggcaa gccagagcat cattgcctac acaatgagtc tgggagcaga gaactctgtg
     2821 gcttacagca acaacagcat tgccatccca accaacttca ccatctctgt gaccacagaa
     2881 attctgcctg tgagtatgac caagacctct gtggactgta caatgtatat ctgtggagac
     2941 agcacagagt gtagcaacct gctgctccaa tatggctcct tctgtaccca acttaagagg
     3001 gctctgacag gcattgctgt ggaacaggac aagaacaccc aggaggtgtt tgcccaggtg
     3061 aagcagattt acaagacacc tccaatcaag tactttggag gcttcaactt cagccagatt
     3121 ctgcctgacc caagcaagcc aagcaagagg tccttcattg aggacctgct gttcaacaag
     3181 gtgaccctgg ctgatgctgg cttcatcaag caatatggag actgtctggg agacattgct
     3241 gcaagggacc tgatttgtgc ccagaagttc aatggactga ccgtgctgcc tccactgctg
     3301 acagatgaga tgattgccca atacacctct gccctgctgg ctggcaccat cacctctggc
     3361 tggacctttg gagcaggagc agccctccaa atcccatttg ctatgcagat ggcttacagg
     3421 ttcaatggca ttggagtgac ccagaatgtg ctctatgaga accagaaact gattgccaac
     3481 cagttcaact ctgccattgg caagattcag gactccctgt ccagcacagc ctctgccctg
     3541 ggcaaactcc aagatgtggt gaaccataat gcccaggctc tgaacaccct ggtgaagcaa
     3601 ctttccagca agtttggagc catctcctct gtgctgaatg acatcctgag cagactggac
     3661 aaggtggagg ctgaggtcca gattgacaga ctgattacag gcagactcca atccctccaa
     3721 acctatgtga cccaacaact tatcagggct gctgagatta gggcatctgc caacctggct
     3781 gccaccaaga tgagtgagtg tgtgctggga caaagcaaga gggtggactt ctgtggcaag
     3841 ggctaccacc tgatgagttt tccacagtct gcccctcatg gagtggtgtt cctgcatgtg
     3901 acctatgtgc ctgcccagga gaagaacttc accacagccc ctgccatctg ccatgatggc
     3961 aaggctcact ttccaaggga gggagtgttt gtgagcaatg gcacccactg gtttgtgacc
     4021 cagaggaact tctatgaacc acagattatc accacagaca acacctttgt gtctggcaac
     4081 tgtgatgtgg tgattggcat tgtgaacaac acagtctatg acccactcca acctgaactg
     4141 gactccttca aggaggaact ggacaaatac ttcaagaacc acaccagccc tgatgtggac
     4201 ctgggagaca tctctggcat caatgcctct gtggtgaaca tccagaagga gattgacaga
     4261 ctgaatgagg tggctaagaa cctgaatgag tccctgattg acctccaaga actgggcaaa
     4321 tatgaacaat acatcaagtg gccatggtac atctggctgg gcttcattgc tggactgatt
     4381 gccattgtga tggtgaccat aatgctgtgt tgtatgacct cctgttgttc ctgtctgaaa
     4441 ggctgttgtt cctgtggctc ctgttgtaag tgactcgagc catggaagct tgatatctaa
     4501 ctgactgaac cggtggtacc aaacggggga ggctaactga aacacggaag gagacaatac
     4561 cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac gggtgttggg
     4621 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg
     4681 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt
     4741 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcagat
     4801 ctgcgcagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     4861 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4921 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4981 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     5041 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     5101 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     5161 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     5221 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     5281 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     5341 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     5401 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     5461 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     5521 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     5581 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     5641 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     5701 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     5761 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     5821 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     5881 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     5941 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     6001 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     6061 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     6121 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     6181 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     6241 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     6301 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     6361 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     6421 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     6481 cgcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
     6541 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     6601 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     6661 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     6721 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     6781 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     6841 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     6901 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     6961 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     7021 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     7081 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     7141 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     7201 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     7261 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     7321 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     7381 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     7441 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     7501 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     7561 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     7621 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     7681 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     7741 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     7801 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     7861 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     7921 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     7981 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     8041 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     8101 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     8161 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     8221 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     8281 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     8341 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     8401 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     8461 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     8521 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     8581 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     8641 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     8701 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     8761 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     8821 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     8881 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     8941 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
     9001 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     9061 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     9121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
     9181 tacgggccag atat
//
