LOCUS       sequence-381650-        9454 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes a wildtype human Pol? (eta) with P2A-tagBFP marker driven by
            CAG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9454)
  AUTHORS   Tong H, Wang X, Liu Y, Liu N, Li Y, Luo J, Ma Q, Wu D, Li J, Xu C, 
            Yang H
  TITLE     Programmable A-to-Y base editing by fusing an adenine base editor 
            with an N-methylpurine DNA glycosylase.
  JOURNAL   Nat Biotechnol. 2023 Jan 9. doi: 10.1038/s41587-022-01595-6.
  PUBMED    36624150
REFERENCE   2  (bases 1 to 9454)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2023 Jan 9. doi: 10.1038/s41587-022-01595-6."
COMMENT     Sequence Label: pCAG-bpNLS-hPol eta-2A-tagBFP-bGHpA
FEATURES             Location/Qualifiers
     source          1..9454
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   250..284
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        585..964
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1244..2260
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2184..2206
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             2340..2360
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4500..4556
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4557..5255
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(5325..5342)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5331..5555
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5740..5802
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5887..5906)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5888..5906)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5913..5930)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5913..5929)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5922..5944)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6070..6498
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6157..6176)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6367..6388
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             6842..7636
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6896..6915)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7506..7525
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(7654..7722,7723..8514)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7872..7891)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8685..9273
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9174..9193
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9427..9444
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccttaac cctagaaaga tagtctgcgt aaaattgacg catgcattct tgaaatattg
      301 ctctctcttt ctaaatagcg cgaatccgtc gctgtgcatt taggacatct cagtcgccgc
      361 ttggagctcc cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt tgaactataa
      421 cgaccgcgtg agtcaaaatg acgcatgatt atcttttacg tgacttttaa gatttaactc
      481 atacgataat tatattgtta tttcatgttc tacttacgtg ataacttatt atatatatat
      541 tttcttgtta tagatatcat taatatcgat ggcgcgccgg attcgacatt gattattgac
      601 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
      661 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      721 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      781 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      841 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      901 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      961 catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
     1021 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
     1081 gggggggcgc gcgccrggsg gggsggggsg gggsgrgggg sggggsgggg sgrggsggrg
     1141 rggkgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg
     1201 gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg
     1261 ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac
     1321 cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg
     1381 cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg aggggctccg
     1441 ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg
     1501 gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg
     1561 gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg
     1621 cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc
     1681 agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc cccgagttgc
     1741 tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt
     1801 gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc ctcgggccgg
     1861 ggrgggctcg ggggrggggc gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc
     1921 gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc
     1981 ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg
     2041 ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc
     2101 gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg acggctgcct
     2161 tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc
     2221 ctctgctaac catgttcatg ccttcttctt tttcctacag atccttaatt aagcacgcgt
     2281 gcgggatccg ctcaaccgcc accatgaaac ggacagccga cggaagcgag ttcgagtcac
     2341 caaagaagaa gcggaaagtc atggctactg gacaggatcg agtggttgct ctcgtggaca
     2401 tggactgttt ttttgttcaa gtggagcagc ggcaaaatcc tcatttgagg aataaacctt
     2461 gtgcagttgt acagtacaaa tcatggaagg gtggtggaat aattgcagtg agttatgaag
     2521 ctcgtgcatt tggagtcact agaagtatgt gggcagatga tgctaagaag ttatgtccag
     2581 atcttctact ggcacaagtt cgtgagtccc gtgggaaagc taacctcacc aagtaccggg
     2641 aagccagtgt tgaagtgatg gagataatgt ctcgttttgc tgtgattgaa cgtgccagca
     2701 ttgatgaggc ttacgtagat ctgaccagtg ctgtacaaga gagactacaa aagctacaag
     2761 gtcagcctat ctcggcagac ttgttgccaa gcacttacat tgaagggttg ccccaaggcc
     2821 ctacaacggc agaagagact gttcagaaag aggggatgcg aaaacaaggc ttatttcaat
     2881 ggctcgattc tcttcagatt gataacctca cctctccaga cctgcagctc accgtgggag
     2941 cagtgattgt ggaggaaatg agagcagcca tagagaggga gactggtttt cagtgttcag
     3001 ctggaatttc acacaataag gtcctggcaa aactggcctg tggactaaac aagcccaacc
     3061 gccaaaccct ggtttcacat gggtcagtcc cacagctctt cagccaaatg cccattcgca
     3121 aaatccgtag tcttggagga aagctagggg cctctgtcat tgagatccta gggatagaat
     3181 acatgggtga actgacccag ttcactgaat cccagctcca gagtcatttt ggggagaaga
     3241 atgggtcttg gctatatgcc atgtgccgag ggattgaaca tgatccagtt aaacccaggc
     3301 aactacccaa aaccattggc tgtagtaaga acttcccagg aaaaacagct cttgctactc
     3361 gggaacaggt acaatggtgg ctgttgcaat tagcccagga actagaggag agactgacta
     3421 aagaccgaaa tgataatgac agggtagcca cccagctggt tgtgagcatt cgtgtacaag
     3481 gagacaaacg cctcagcagc ctgcgccgct gctgtgccct tacccgctat gatgctcaca
     3541 agatgagcca tgatgcattt actgtcatca agaactgtaa tacttctgga atccagacag
     3601 aatggtctcc tcctctcaca atgcttttcc tctgtgctac aaaattttct gcctctgccc
     3661 cttcatcttc tacagacatc accagcttct tgagcagtga cccaagttct ctgccaaagg
     3721 tgccagttac cagctcagaa gctaagaccc agggaagtgg cccagcggtg acagccacta
     3781 agaaagcaac cacgtctctg gaatcattct tccaaaaagc tgcagaaagg cagaaagtta
     3841 aagaagcttc gctttcatct cttactgctc ccactcaggc tcccatgagc aattcaccat
     3901 ccaagccctc attacctttt caaaccagtc aaagtacagg aactgagccc ttctttaagc
     3961 agaaaagtct gcttctaaag cagaaacagc ttaataattc ttcagtttct tccccccaac
     4021 aaaacccatg gtccaactgt aaagcattac caaactcttt accaacagag tatccagggt
     4081 gtgtccctgt ttgtgaaggg gtgtcgaagc tagaagaatc ctctaaagca actcctgcag
     4141 agatggattt ggcccacaac agccaaagca tgcacgcctc ttcagcttcc aaatctgtgc
     4201 tggaggtgac tcagaaagca accccaaatc caagtcttct agctgctgag gaccaagtgc
     4261 cctgtgagaa gtgtggctcc ctggtaccgg tatgggatat gccagaacac atggactatc
     4321 attttgcatt ggagttgcag aaatcctttt tgcagcccca ctcttcaaac ccccaggttg
     4381 tttctgccgt atctcatcaa ggcaaaagaa atcccaagag ccctttggcc tgcactaata
     4441 aacgccccag gcctgagggc atgcaaacat tggaatcatt ttttaagcca ttaacacatg
     4501 ctactaactt cagcctgctg aagcaggctg gcgacgtgga ggagaaccct ggacctatga
     4561 gcgagctgat taaggagaac atgcacatga agctgtatat ggagggcacc gtggacaacc
     4621 atcacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga
     4681 gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctactagct
     4741 tcctctacgg cagcaagacc ttcatcaacc acacccaggg catccccgac ttcttcaagc
     4801 agtccttccc tgagggcttc acatgggaga gagtcaccac atacgaggac gggggcgtgc
     4861 tgaccgctac ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca
     4921 gaggggtgaa cttcacatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg
     4981 ccttcaccga gacactgtac cccgctgacg gcggcctgga aggcagaaac gacatggccc
     5041 tgaagctcgt gggcgggagc catctgatcg caaacatcaa gaccacatat agatccaaga
     5101 aacccgctaa gaacctcaag atgcctggcg tctactatgt ggactacaga ctggaaagaa
     5161 tcaaggaggc caacaacgag acatacgtcg agcagcacga ggtggcagtg gccagatact
     5221 gcgacctccc tagcaaactg gggcacaagc tgaatcttaa ctgatctagt cctggactag
     5281 cgctgtcgac gccggttagg ggcccgttta aacccgctga tcagcctcga ctgtgccttc
     5341 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     5401 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     5461 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     5521 tagcaggcat gctggggatg cggtgggctc tatggcggcg cgcctaaaag ttttgttact
     5581 ttatagaaga aattttgagt ttttgttttt ttttaataaa taaataaaca taaataaatt
     5641 gtttgttgaa tttattatta gtatgtaagt gtaaatataa taaaacttaa tatctattca
     5701 aattaataaa taaacctcga tatacagacc gataaaacac atgcgtcaat tttacgcatg
     5761 attatcttta acgtacgtca caatatgatt atctttctag ggttaaggcc ggccaataag
     5821 ggcgaattct gcagatatcc atcacactgg cggccgctcg agcatgcatc tagagggccc
     5881 aattcgccct atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac
     5941 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     6001 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     6061 ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     6121 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     6181 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     6241 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     6301 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     6361 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     6421 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     6481 aaaaatttaa cgcgaatttt aacaaaattc agggcgcaag ggctgctaaa ggaagcggaa
     6541 cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg atgaatgtca gctactgggc
     6601 tatctggaca agggaaaacg caagcgcaaa gagaaagcag gtagcttgca gtgggcttac
     6661 atggcgatag ctagactggg cggttttatg gacagcaagc gaaccggaat tgccagctgg
     6721 ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt tcttgccgcc
     6781 aaggatctga tggcgcaggg gatcaagatc tgatcaagag acaggatgag gatcgtttcg
     6841 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     6901 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     6961 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     7021 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     7081 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     7141 ggatctcctg tcatcccacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     7201 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     7261 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     7321 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     7381 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     7441 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     7501 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     7561 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     7621 tgacgagttc ttctgaattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     7681 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     7741 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     7801 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     7861 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     7921 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     7981 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     8041 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     8101 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     8161 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     8221 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     8281 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     8341 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     8401 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     8461 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     8521 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     8581 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     8641 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     8701 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     8761 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     8821 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     8881 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     8941 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     9001 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     9061 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     9121 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     9181 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     9241 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     9301 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     9361 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     9421 gagcgcagcg agtcagtgag cgaggaagcg gaag
//
