LOCUS       sequence-38172-e         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Dewaste V, Pouillon V, Moreau C, Shears S, Takazawa K, Erneux C
  TITLE     Cloning and expression of a cDNA encoding human inositol 
            1,4,5-trisphosphate 3-kinase C.
  JOURNAL   Biochem J. 2000 Dec 1;352 Pt 2:343-51.
  PUBMED    11085927
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem 
            J."; date: "2000-12-1"; volume: "352 Pt 2"; pages: "343-51"
COMMENT     Sequence Label: pcDNA3-IP3KC
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             123..140
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             144..176
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(180..188,189..203)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctggctag cgtttaaact taagcttacc atggggggtt
      121 ctcatcatca tcatcatcat ggtatggcta gcatgactgg tggacagcaa atgggtcggg
      181 atctgtacga cgatgacgat aaggtaccag gatccggcat gaggcgctgc ccgtgccgtg
      241 ggagcctgaa cgaggcggag gccggggcgc tgcccgcggc ggcccgcatg ggactggagg
      301 cgccgcgagg agggcggcgg cggcagccgg gacagcagcg acctgggccc ggcgcagggg
      361 ccccggcggg gcggccggag gggggcgggc cctgggcccg gacagagggg tccagcctcc
      421 acagcgagcc tgagagggcc ggcctcgggc ctgcgccggg gacagagagt ccgcaggcag
      481 aattctggac agacggacag actgagcccg cggcagctgg ccttggagta gagaccgaga
      541 ggcccaagca aaagacggag ccagacaggt ccagcctccg gacgcatcta gaatggagct
      601 ggtcagagct ggagacgact tgtctttgga cggagaccgg gacagatggc ctttggactg
      661 atccgcacag gtccgacctc cagtttcagc ccgaggaggc cagcccctgg acacagccag
      721 gggttcatgg gccctggaca gagctggaaa cgcatgggt
//
