LOCUS       sequence-381720-        9690 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of RIOK3 (human) tagged with N-terminal 
            Flag for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9690)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 9690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHAGE-CMV-Flag-RIOK3-STOP_IDG-K
FEATURES             Location/Qualifiers
     source          1..9690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             5..638
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    685..810
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1307..1540
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1725..1769
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2062..2179
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2231..2534
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2535..2738
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2689..2709
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2735..2759
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2830..2839
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2839..2862
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    2872..2896
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4463..4487)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          4561..4790
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     4773..4792
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(4863..5437,5438..5440,5441..5449)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5030..5047)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5257..5276
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5469..6068
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5469..5488)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5965..5985
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6083..6671
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6136..6156)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6554..6565)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6746..6979
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7098..7116)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7184..7288
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7289..7357,7358..8149)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7507..7526)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8320..8908
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8809..8828
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9062..9079
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9320..9341
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9356..9361,9362..9379,9380..9386)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9394..9410
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9399..9421
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9418..9434
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9418..9434
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9484..9503
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
       61 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
      121 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
      181 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
      241 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
      301 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
      361 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
      421 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
      481 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
      541 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
      601 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
      661 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
      721 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
      781 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
      841 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
      901 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
      961 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     1021 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     1081 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     1141 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     1201 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     1261 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     1321 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     1381 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     1441 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     1501 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     1561 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     1621 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     1681 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     1741 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     1801 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     1861 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     1921 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     1981 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc cgaattcaca
     2041 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     2101 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     2161 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggactag
     2221 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2281 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2341 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2401 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2461 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2521 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2581 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2641 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     2701 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     2761 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccggcgg ccataccggt
     2821 cagctcgagg ccaccatgga ctacaaggac gacgacgaca agggcggatc aacaagtttg
     2881 tacaaaaaag caggctccac catggatctg gtaggagtgg catcgcctga gcccgggacg
     2941 gcagcggcct ggggacccag caagtgtcca tgggctattc ctcaaaatac aatatcttgt
     3001 tctttggctg atgtaatgag tgaacagctg gccaaagaat tgcagttaga agaagaagct
     3061 gccgtttttc ctgaagttgc tgttgctgaa ggaccattta ttactggaga aaacattgat
     3121 acttccagtg accttatgct ggctcagatg ctacagatgg aatatgacag agaatatgat
     3181 gcacagctta ggcgtgaaga aaaaaaattc aatggagata gcaaagtttc catttccttt
     3241 gaaaattatc gaaaagtgca tccttatgaa gacagcgata gctctgaaga tgaggttgac
     3301 tggcaggata ctcgtgatga tccctacaga ccagcaaaac cggttcccac tcctaaaaag
     3361 ggctttattg gaaaaggaaa agatatcacc accaaacatg atgaagtagt atgtgggaga
     3421 aagaacacag caagaatgga aaattttgca cctgagtttc aggtaggaga tggaattgga
     3481 atggatttaa aactatcaaa ccatgttttc aatgctttaa aacaacatgc ctactcagaa
     3541 gaacgtcgaa gtgcccgcct acatgagaaa aaggagcatt ctacagcaga aaaagcagtt
     3601 gatcctaaga cacgtttact tatgtataaa atggtcaact ctggaatgtt ggagacaatc
     3661 actggctgta ttagtacagg aaaggagtct gttgtctttc atgcatatgg agggagcatg
     3721 gaggatgaaa aggaagatag taaagttata cctacagaat gtgccatcaa ggtatttaaa
     3781 acaaccctta atgaatttaa gaatcgtgac aaatatatta aagatgattt caggtttaaa
     3841 gatcgcttca gtaaactaaa tccacgtaag atcatccgca tgtgggcaga aaaagaaatg
     3901 cacaatctca caagaatgca gagagctgga attccttgtc caacagttgt actactgaag
     3961 aaacacattt tagttatgtc ttttattggc catgatcaag ttccagcccc taaattaaaa
     4021 gaagtaaagc tcaatagtga agaaatgaaa gaagcctact atcaaactct tcatttgatg
     4081 cggcagttat atcatgaatg tacgcttgtc catgctgacc tcagtgagta taacatgctg
     4141 tggcatgctg gaaaggtctg gttgatcgat gtcagtcagt cagtagaacc tacccaccct
     4201 cacggcctgg agttcttgtt ccgggactgc aggaatgtct cgcagttttt ccagaaagga
     4261 ggagtcaagg aagcccttag tgaacgagaa ctcttcaatg ctgtttcagg cttaaacatc
     4321 acagcagata atgaagctga ttttttagct gagatagaag ctttggagaa aatgaatgaa
     4381 gatcacgttc agaagaatgg aaggaaagct gcttcatttt tgaaagatga tggagaccca
     4441 ccactactat atgatgaata ggacccagct ttcttgtaca aagtggttga ttaagatcca
     4501 ctagtaacgg ccgccagtgt gctggaatta attcgctgtc tgcgagggcc agctgttggg
     4561 gtgagtactc cctctcaaaa gcgggcatga cttctgcgct aagattgtca gtttccaaaa
     4621 acgaggagga tttgatattc acctggcccg cggtgatgcc tttgagggtg gccgcgtcca
     4681 tctggtcaga aaagacaatc tttttgttgt caagcttgag gtgtggcagg cttgagatct
     4741 ggccatacac ttgagtgaca atgacatcca ctttgccttt ctctccacag gtgtccactc
     4801 ccaggtccaa ctgcaggtcg agcatgcatc tagggcggcc aattccgccc ctctcccccc
     4861 cccccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     4921 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     4981 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     5041 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     5101 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     5161 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     5221 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     5281 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     5341 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     5401 ggggacgtgg ttttcctttg aaaaacacga tgataagctt gccacaaccc acaaggagac
     5461 gaccttccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc
     5521 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     5581 acccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     5641 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     5701 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     5761 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     5821 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     5881 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     5941 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     6001 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     6061 gtgcctgata tcgatagatc ctaatcaacc tctggattac aaaatttgtg aaagattgac
     6121 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     6181 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     6241 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     6301 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     6361 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     6421 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     6481 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     6541 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     6601 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     6661 cgcctccccg cctgagatcc tttaagacca atgacttaca aggcagctgt agatcttagc
     6721 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     6781 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     6841 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     6901 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     6961 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     7021 gcaaagaaat gaatatcaga gagtgagagg cccgggttaa ttaaggaaag ggctagatca
     7081 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     7141 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     7201 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     7261 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     7321 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     7381 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     7441 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     7501 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
     7561 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     7621 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     7681 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     7741 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     7801 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     7861 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     7921 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     7981 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     8041 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     8101 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     8161 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     8221 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     8281 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     8341 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     8401 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     8461 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     8521 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     8581 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     8641 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     8701 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     8761 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     8821 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     8881 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     8941 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     9001 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     9061 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     9121 gcgttggccg attcattaat gcagcaagct catggctgac taattttttt ttatttatgc
     9181 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     9241 aggcctaggc ttttgcaaaa agctccccgt ggcacgacag gtttcccgac tggaaagcgg
     9301 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     9361 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     9421 gaaacagcta tgacatgatt acgaatttca caaataaagc atttttttca ctgcattcta
     9481 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc
     9541 aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc
     9601 tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt
     9661 gtatttgtta aatatgtact acaaacttag
//
