LOCUS       sequence-381731-        6007 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ApoE2 in Mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Hudry E, Dashkoff J, Roe AD, Takeda S, Koffie RM, Hashimoto T, 
            Scheel M, Spires-Jones T, Arbel-Ornath M, Betensky R, Davidson BL, 
            Hyman BT
  TITLE     Gene transfer of human Apoe isoforms results in differential 
            modulation of amyloid deposition and neurotoxicity in mouse brain.
  JOURNAL   Sci Transl Med. 2013 Nov 20;5(212):212ra161. doi: 
            10.1126/scitranslmed.3007000.
  PUBMED    24259049
REFERENCE   2  (bases 1 to 6007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Transl 
            Med."; date: "2013-11-20"; volume: "5(212):212ra161. doi"; pages: " 
            10.1126/scitranslmed.3007000"
COMMENT     Sequence Label: pCMV4-ApoE2
FEATURES             Location/Qualifiers
     source          1..6007
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     179..199
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     polyA_signal    223..845
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     627..646
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     promoter        complement(2119..2322)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2148..2168)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2323..2702
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(2863..2880)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2863..2879)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2872..2894)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(3020..3475)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3157..3178)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3369..3388
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3502..3606
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3607..3675,3676..4467)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3825..3844)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4638..5226
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5127..5146
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5380..5397
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5514..5535
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5550..5555,5556..5573,5574..5580)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5588..5604
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5593..5615
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5612..5628
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5612..5628
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5642..5661
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5642..5660
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          5737..5833
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     promoter        complement(join(5872..6007,1..194))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(5886..6007,1..14)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 gttaggggcg ggatgggcgg agttaggggc gggactatgg ttgctgacta attgagatgc
       61 atgctttgca tacttctgcc tgctggggag cctggggact ttccacacct ggttgctgac
      121 taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac
      181 cctaactgac acacattcca cagccaagct ggccgcgtac ccaattcaac aggcatctac
      241 tgagtggacc caacgcatga gaggacagtg ccaagcaagc aactcaaatg tcccaccggt
      301 tgggcatggc caggtagcct atgctgtgtc tggacgtcct cctgctggta tagttatttt
      361 aaaatcagaa ggacagggaa gggagcagtg gttcacgcct gtaatcccag caatttggga
      421 ggccaaggtg ggtagatcac ctgagattag gagttggaga ccagcctggc caatatggtg
      481 aaaccccgtc tctaccaaaa aaacaaaaat tagctgagcc tggtcatgca tgcctggaat
      541 cccaacaact cgggaggctg aggcaggaga atcgcttgaa cccaggaggc ggagattgca
      601 gtgagccaag attgtgccac tgcactccag cttggttccc aatagacccc gcaggcccta
      661 caggttgtct tcccaacttg ccccttgctc cataccaccc ccctccaccc cataatatta
      721 tagaaggaca cctagtcaga caaaatgatg caacttaatt ttattaggac aaggctggtg
      781 ggcactggag tggcaacttc cagggccagg agaggcactg gggaggggtc acagggatgc
      841 cacccgggat cctctagagt cgacctgcag gcatgcaagc ttgcatgcct gcaggtcgac
      901 tctagaggat ccccgggaat ctttattaaa ctagggtcca ccccaggagg acggctgggg
      961 cggggacagg gtctcccgct gcaggctgcg cggaggcagg aggcacgggg tggcgtgggg
     1021 tcgcatggct gcaggcttcg gcgttcagtg attgtcgctg ggcacagggg cggcgctggt
     1081 gcccacggca gcctgcacct tctccaccag cccggcccac tggcgctgca tgtcttccac
     1141 caggggctcg aaccagctct tgaggcgggc ctggaaggcc tcggcctgca ggcgtatctg
     1201 ctgggcctgc tcctccagct tggcgcgcac ctccgccacc tgctccttca cctcgtccag
     1261 gcggtcgcgg gtccggctgc ccatctcctc catccgcgcg cgcagccgct cgccccaggc
     1321 ctgggcccgc tcctgtagcg gctggccggc cagggagccc acagtggcgg cccgcacgcg
     1381 gccctgttcc accaggggcc ccaggcgctc gcggatggcg ctgaggccgc gctcggcgcc
     1441 ctcgcgggcc ccggcctggt acactgccag gcacttctgc aggtcatcgg catcgcggag
     1501 gagccgctta cgcagcttgc gcaggtggga ggcgaggcgc acccgcagct cctcggtgct
     1561 ctggccgagc atggcctgca cctcgccgcg gtactgcacc aggcggccgc acacgtcctc
     1621 catgtccgcg cccagccggg cctgcgccgc ctgcagctcc ttggacagcc gtgcccgcgt
     1681 ctcctccgcc accggggtca gttgttcctc cagttccgat ttgtaggcct tcaactcctt
     1741 catggtctcg tccatcagcg ccctcagttc ctgggtgacc tgggagctga gcagctcctc
     1801 ctgcacctgc tcagacagtg tctgcaccca gcgcaggtaa tcccaaaagc gacccagtgc
     1861 cagttcccag cgctggccgc tctgccactc ggtctgctgg cgcagctcgg gctccggctc
     1921 tgtctccacc gcttgctcca ccttggcctg gcatcctgcc aggaatgtga ccagcaacgc
     1981 agcccacaga accttcatct tcctgcctgt gattggccag tcggctcctg gggaaggccc
     2041 cgggtaccag atctgaattc gatggaagta tttgaaagaa aattaaaaat aaaaacaatt
     2101 ctgacggttc actaaacgag ctctgcttat atagacctcc caccgtacac gcctaccgcc
     2161 catttgcgtc aacggggcgg ggttattacg acattttgga aagtcccgtt gattttggtg
     2221 ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa
     2281 ccgctatcca cgcccattgg tgtactgcca aaaccgcatc accatggtaa tagcgatgac
     2341 taatacgtag atgtactgcc aagtaggaaa gtcccgtaag gtcatgtact gggcataatg
     2401 ccaggcgggc catttaccgt cattgacgtc aatagggggc ggacttggca tatgatacac
     2461 ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa
     2521 agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc
     2581 gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata
     2641 tgggctatga actaatgacc ccgtaattga ttactattaa taactagtca ataatcaatg
     2701 tcaacatggc ggtcatattg gacatgagcc aatataaatg tacatattat gatatagata
     2761 caacgtatgc aatggccaat agccaatatt gatttatgct atataaccaa tgactaatat
     2821 ggctaattgc caatattgat tcaatgtata gatcagcttg gcactggccg tcgttttaca
     2881 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     2941 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     3001 cagcctgaat ggcgaatgga aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
     3061 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     3121 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     3181 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     3241 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     3301 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     3361 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     3421 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt
     3481 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     3541 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     3601 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     3661 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     3721 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     3781 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     3841 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     3901 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     3961 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     4021 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     4081 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     4141 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     4201 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     4261 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     4321 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     4381 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     4441 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     4501 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     4561 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     4621 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     4681 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     4741 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     4801 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     4861 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     4921 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     4981 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     5041 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     5101 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     5161 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     5221 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     5281 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     5341 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     5401 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     5461 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     5521 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     5581 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat
     5641 ttaatacgac tcactatagg gaattcgagc tcggtacgcg gccctagagt cgacctgcag
     5701 cagcttttag agcagaagta acacttccgt acaggcctag aagtaaaggc aacatccact
     5761 gaggagcagt tctttgattt gcaccaccac cggatccggg acctgaaata aaagacaaaa
     5821 agactaaact taccagttaa ctttctggtt tttcagttcc tcgaggagct ttttgcaaaa
     5881 gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc
     5941 ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact
     6001 gggcgga
//
