LOCUS       sequence-381732-       16665 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the fluorescently labeled dynein motor domain fused to 
            dimerization domain FKBP to recruit it to the plasma membrane by 
            using the chemical dimerization system FKBP-FRB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16665)
  AUTHORS   Schelski M, Bradke F
  TITLE     Microtubule retrograde flow retains neuronal polarization in a 
            fluctuating state.
  JOURNAL   Sci Adv. 2022 Nov 4;8(44):eabo2336. doi: 10.1126/sciadv.abo2336. 
            Epub 2022 Nov 4.
  PUBMED    36332023
REFERENCE   2  (bases 1 to 16665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2022-11-4"; volume: "8(44):eabo2336. doi"; pages: " 
            10.1126/sciadv.abo2336. Epub 2022 Nov 4"
COMMENT     Sequence Label: pBetaActin-FKBP-mScarlet-Dync1h1motor
FEATURES             Location/Qualifiers
     source          1..16665
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    150..284
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(187..206)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     241..260
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(298..314)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(298..314)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(311..333)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    322..338
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(346..352,353..370,371..376))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    391..412
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(529..546)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(700..1288)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(780..799)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1459..2250,2251..2319))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2082..2101
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2320..2424)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2492..2510
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2548..2570)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2670..2689
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2883..2905
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2897..2914
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2898..2914
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4715..4990
                     /label=chicken beta-actin promoter
     primer_bind     5933..5955
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             join(6007..6009,6010..6330)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             6352..7044
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGMDELYK"
     primer_bind     complement(6482..6500)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6750..6769
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     regulatory      11014..11023
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 acggtaccgc gggcccggga tccaccggat ctagataagt aatgatcata atcagccata
       61 tcacatctgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
      121 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
      181 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
      241 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaattcg taatcatgtc
      301 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
      361 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
      421 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
      481 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
      541 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
      601 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
      661 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
      721 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
      781 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
      841 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
      901 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
      961 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     1021 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     1081 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     1141 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     1201 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     1261 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     1321 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     1381 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     1441 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     1501 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     1561 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     1621 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     1681 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     1741 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     1801 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     1861 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     1921 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     1981 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     2041 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     2101 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     2161 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     2221 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     2281 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     2341 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     2401 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     2461 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     2521 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     2581 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     2641 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     2701 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
     2761 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     2821 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     2881 ttcccagtca cgacgttgta aaacgacggc cagtgccaag ttgggatctt tgcattggcc
     2941 cacggctctc aggatgggga tgctcccctt cagcacccgg ttccccttgg aaactgatgg
     3001 tcctggctct gtggcatggc agtggcactg tgaggagccc cctaccagca gcacacagtg
     3061 gggttggcac tgccacgctc cggatgccgc gctctgatcc aaccccataa tcaagggaac
     3121 ccgaattgcc ccatcattgc ccccaccacc cccatcctgc cgggccctca caccccacgc
     3181 tgccttctgg tgacattccc cagcccaaac ccacggcttc atggctaccg cggggcattt
     3241 cccattgccg ccccattatc agctctgcac acctcccgct gtacccatgc ctcgtggctg
     3301 cccttctttg acgtataatc ttctaattaa tacccggcct tgtcaaagtg gagcacaaac
     3361 gttaattaat tccccagcag gcaggtaatt aacagtgtga ctcccttttt gctgcgagtg
     3421 gggctgatac agagagatgt ggcactatgg agcccacggg gtcctggcac tgggtgccca
     3481 cggaggtccc catgtgctgc agtgtcaccg cctccgagga gacagtattg tccctgcggt
     3541 gtccctgcag ctcagctctg tccacagggc cacctccagt ttggagggga cacaatgcag
     3601 ccccgatgca acccatcctc gcagcatccc agggacaaag accccactgc aagaccgcac
     3661 acagggctgg gtcccgctcc cctaatatct acagtgcttc ttgcatggcc ccttaatcaa
     3721 tgcagttaat cagcatgcgc tcatgcaccg ctctggagct gcaaagcccc tcgcagcgct
     3781 gctcaccaac accgcgcacc gccccggccc agcctgcagc acgcgctgca aacaggaaag
     3841 aaacaaaata ttgcccaaat gtaggcaaag gcattcggct gccttgacct ccgccgggcc
     3901 gggccctgcc tgactcagct ccttactcag cgctcgcttc ctccctccgg ctgccaccgc
     3961 cgcagcgcac accctgacaa agagtggccc ttaacgggct ctgaggtgca cccagcagtg
     4021 cactcagcag tccaagggcc ggcctggagg tttgcaccgc tacgtgctga cattagcatt
     4081 gaacttggcc ctgggtagtg ctgcaggccg ggcggggtgg gtgtagagag tgcagcgcgc
     4141 gttgcacccg gtgccccttc ccctcccttg catcccagca ggctgcaccc cagcaccagg
     4201 cccgtgcatg catgctcctg gtgttattgc agcctggtgc atgcatgcgt cttgatggtg
     4261 cagcgctgtg catgcatcct ccttggtgtg tagcagtcta gtgcatgcat acccctcggt
     4321 gttattgctg ctctgtgcac gcacgctcat tgtatcactt catcccagtg catgcactca
     4381 cactggagcg attgctgctc ggtgcacgca cactcattgt atcactgcag ctcagtggct
     4441 gcacgcacac cggtgttatt gctgctcggt gcgtgcatgc acatcagtgt cgctgcagct
     4501 cagtgcatgc acgctcattg cccatcgcta tccctgcctc tcctgctggc gctccccggg
     4561 aggtgacttc aaggggaccg caggaccacc tcgggggtgg ggggagggct gcacacgcgg
     4621 accccgctcc ccctccccaa caaagcactg tggaatcaaa aaggggggag gggggatgga
     4681 ggggcgcgtc acacccccgc cccacaccct cacctcgagg tgagccccac gttctgcttc
     4741 actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta
     4801 ttttgtgcag cgatgggggc gggggggggg ggggcgcgcg ccrggsgggg sggggsgggg
     4861 sgrggggsgg ggsggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct
     4921 ccgaaagttt ccttttatgg cgrggcggcg gcggcggcgg ccctataaaa agcgaagcgc
     4981 gcggcgggcg ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc
     5041 gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg
     5101 cccttctcct ccgggctgta attagcgctt ggtttaatga cggctcgttt cttttctgtg
     5161 gctgcgtgaa agccttaaag ggctccggga gggccctttg tgcggggggg agcggctcgg
     5221 ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg
     5281 gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga
     5341 gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg
     5401 tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg cgcggcggtc gggctgtaac
     5461 ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc
     5521 cgtgcggggc gtggcgcggg gctcgccgtg ccgggcgggg ggkggcggca ggkgggggkg
     5581 ccgggcgggg cggggccgcc ycgggccggg grgggctcgg gggrggggcg cggcggcccc
     5641 cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg
     5701 tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg
     5761 cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa
     5821 atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctcca tctccagcct
     5881 cggggctgtc cgcaggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc
     5941 ttctggcgtg tgaccggcgg ggtttatatc ttcccttctc tgttcctccg cagcccccaa
     6001 gctagcatgg gagtgcaggt ggaaaccatc tccccaggag acgggcgcac cttccccaag
     6061 cgcggccaga cctgcgtggt gcactacacc gggatgcttg aagatggaaa gaaatttgat
     6121 tcctcccggg acagaaacaa gccctttaag tttatgctag gcaagcagga ggtgatccga
     6181 ggctgggaag aaggggttgc ccagatgagt gtgggtcaga gagccaaact gactatatct
     6241 ccagattatg cctatggtgc cactgggcac ccaggcatca tcccaccaca tgccactctc
     6301 gtcttcgatg tggagcttct aaaactggaa ggaagttcag gaggttctag tgtgagcaag
     6361 ggcgaggcag tgatcaagga gttcatgcgg ttcaaggtgc acatggaggg ctccatgaac
     6421 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     6481 gccaagctga aggtgaccaa gggtggcccc ctgcccttct cctgggacat cctgtcccct
     6541 cagttcatgt acggctccag ggccttcacc aagcaccccg ccgacatccc cgactactat
     6601 aagcagtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
     6661 gccgtgaccg tgacccagga cacctccctg gaggacggca ccctgatcta caaggtgaag
     6721 ctccgcggca ccaacttccc tcctgacggc cccgtaatgc agaagaagac aatgggctgg
     6781 gaagcgtcca ccgagcggtt gtaccccgag gacggcgtgc tgaagggcga cattaagatg
     6841 gccctgcgcc tgaaggacgg cggccgctac ctggcggact tcaagaccac ctacaaggcc
     6901 aagaagcccg tgcagatgcc cggcgcctac aacgtcgacc gcaagttgga catcacctcc
     6961 cacaacgagg actacaccgt ggtggaacag tacgaacgct ccgagggccg ccactccacc
     7021 ggcggcatgg acgagctgta caagtctggt ggatcaagtg gtggaggcga gatggccttg
     7081 gaagaatttt tgaagcagat acgagaagtg tggaacacat acgaactaga cttggtcaac
     7141 tatcagaaca aatgccgcct catccgaggc tgggatgacc tcttcaacaa ggtcaaggag
     7201 catatcaaca gcgtctctgc catgaaactc tctccctatt acaaggtttt tgaagaagat
     7261 gccctgagct gggaggacaa gctgaaccga atcatggctc tctttgacgt gtggattgac
     7321 gtgcagaggc gctgggtcta cttggaaggc atcttcacag gcagtgcgga catcaaacat
     7381 ctgctgcctg tggaaaccca gcgcttccag agtatcagca cggagttttt ggctctgatg
     7441 aaaaaagtct ccaagtctcc acttgtgatg gatgttctga acatacaggg agtccagagg
     7501 tctctggaaa ggttggcaga cctgctgggg aagatccaga aagcattggg ggagtacctg
     7561 gaacgggagc gctcgtcttt ccccaggttc tactttgtgg gtgatgagga tttgcttgag
     7621 atcattggga atagcaagaa cgtggctaag ttgcagaagc acttcaagaa aatgttcgcc
     7681 ggcgtttcca gcatcatctt aaatgaggat aactctgtgg tcctcggcat ctcatcccga
     7741 gaaggggagg aggttatgtt taaaactcca gtgtccatca ctgaacaccc caaaatcaat
     7801 gagtggctca ctctggtgga aaaggagatg agagtcacct tggctaaact tctggcagag
     7861 tctgttacag aagtggagat ttttggaaaa gcaacttcta ttgacccaaa tacctacatt
     7921 acttggattg ataaatacca ggcccagcta gtggtattgt cagcccagat agcctggtcc
     7981 gagaacgtgg agaatgcgct gagcaacgtt gggggaggtg gtgatgtggg gcccttgcag
     8041 tctgtgctga gcaatgtgga ggtcacactc aacgtcttag cggactctgt cctcatggag
     8101 cagcctcctc tccggagacg gaagctggag cacttgatca cagagctggt tcaccaaaga
     8161 gacgttacca ggtccttgat caaaagcaag atcgacaacg ccaaatcttt cgaatggctc
     8221 agccagatgc gattttactt tgaccctaag caaacggacg tgttacagca gctgtccatt
     8281 cagatggcca atgccaagtt taactacggc tttgagtacc tgggtgttca agacaagctg
     8341 gttcagacgc ccctcactga ccgttgctat ttgacaatga cacaagcttt ggaggccagg
     8401 cttgggggct ccccatttgg acctgctgga accggaaaga cagagtccgt caaagccctc
     8461 ggccatcagc tggggcggtt cgtcttagtt ttcaactgtg acgaaacctt tgatttccag
     8521 gcgatgggac gtatctttgt ggggctttgc caagtgggcg cgtggggctg ctttgatgag
     8581 tttaaccgcc tggaggagcg gatgctctca gctgtatccc agcaggtgca gtgcatacaa
     8641 gaggcactgc gggagcattc caaccccaac tacgacaaga cctcagcccc cattacttgt
     8701 gagctgctga acaaacaagt caaggttagc ccagacatgg ctatcttcat caccatgaac
     8761 ccaggctatg caggtcgctc aaacctcccc gacaacctca agaagctctt ccggagcttg
     8821 gcgatgacca agcctgaccg gcagctgatc gcccaggtca tgctgtactc gcagggcttc
     8881 cggactgctg aggtccttgc caacaagatc gtcccgttct tcaaactgtg tgatgagcaa
     8941 ctgtcttctc aaagccatta tgactttggg cttcgggctt tgaagagtgt gcttgtaagt
     9001 gcaggcaatg tgaagaggga gaggatccag aagataaaga gggagaaaga agaacggggg
     9061 gaagcagttg atgaaggaga gattgctgag aacctacctg agcaggagat tctgatccag
     9121 agtgtctgcg agacaatggt gcccaagctg gtggcagaag atatcccgct gctcttcagc
     9181 ctcctgtctg acgtgtttcc tggagtccag taccaccgtg gggagatgac agcccttcga
     9241 gaagagctga agaaagtgtg tcaggagatg tacttgacat atggcgacgg agaagaagtt
     9301 ggcgggatgt gggttgaaaa ggttctccag ctctatcaga tcacgcagat taaccacggc
     9361 ctgatgatgg ttgggccttc aggaagtggg aagagcatgg cctggcgagt gctgctgaag
     9421 gctctagaga ggcttgaggg tgtggagggc gtggcccaca taattgaccc caaggccatc
     9481 agcaaagacc acctctacgg aaccttggat cccaacacca gggagtggac agacggactc
     9541 ttcacacacg tgctgagaaa gatcatagac aatgtgagag gtgagctgca gaaacgccag
     9601 tggattgtct ttgatggcga tgtggacccc gagtgggtgg agaacctgaa ctccgtgctg
     9661 gatgacaaca agctcctgac gctgcctaat ggagagcgcc ttagcctgcc tcccaatgtg
     9721 cgaataatgt ttgaagtgca ggacttgaaa tacgcgacgc tggccacggt gtctcgctgt
     9781 ggcatggtgt ggttcagtga ggacgtcctg agcacagaca tgatcttcaa taacttcctg
     9841 gccaggctgc gcagcattcc cctggatgaa ggggaggacg aggcacagcg taggcgaaaa
     9901 ggcaaagaag acgagggcga agaggccgct tctccaatgc tgcagattca aagagatgca
     9961 gcaacgatca tgcagccgta cttcacgtcc aacggcctgg tcaccaaggc gctggagcac
    10021 gccttcaagc tggagcacat catggacctg acgcgcctgc gctgcctggg ctcgctcttc
    10081 tccatgctgc accaggcctg ccgcaacgtg gcgcagtaca atgccaacca tcctgatttc
    10141 cccatgcaga ttgagcagct ggagcgctac attcagcgct atctggttta cgccatcctc
    10201 tggtccttat ctggagacag ccggctgaag atgagagcag agctgggcga gtacatccgg
    10261 aggatcacca ctgtgccgct gcccactgcg cccaacgtcc ccatcattga ctatgaggtg
    10321 tccatcagtg gagagtggtc gccgtggcag gccaaggtgc ctcagattga agtggagaca
    10381 cacaaggtgg cagccccaga tgtcgtcgtg ccaaccttag atacagtccg ccatgaagct
    10441 ctcctgtaca cttggctggc tgaacacaaa cccctggtct tatgtggccc tcctgggtct
    10501 ggcaagacca tgacactctt cagtgctctc cgggccttgc ctgacatgga ggttgtgggc
    10561 ctaaacttct caagtgccac tacaccagag ctgttgctga agacgtttga ccactactgc
    10621 gaatacaggc gtacacctaa cggtgttgtc ttggctcctg ttcagcttgg aaagtggctg
    10681 gtcttgttct gtgatgaaat caacttgccc gatatggata aatacggcac acagagagtc
    10741 atctccttca tcagacagat ggtggagcat ggaggcttct atcgtacctc agaccaaacg
    10801 tgggtaaagc tagagagaat ccagtttgtt ggggcttgca atccacccac tgaccctggg
    10861 agaaagcccc tctcacacag gttcttgcgc cacgtgcctg ttgtatatgt ggattaccca
    10921 ggaccagcct ccctgacgca aatctatggc acttttaacc gcgccatgct gaggctcatt
    10981 ccctcgctcc gtacctatgc agaacccctc actgccgcca tggtggagtt ctataccatg
    11041 tcacaggagc ggtttactca ggacacacag ccacactata tctactcgcc ccgtgagatg
    11101 actcggtggg taagaggaat ctttgaagca ctgagacccc tggagacatt gcctgttgaa
    11161 ggtctcattc gtatttgggc acatgaagcc ctccgtctct tccaagacag gcttgtggag
    11221 gatgaggagc ggcgctggac tgacgaaaac attgacatgg ttgccttgaa gcacttccct
    11281 aacatagaca aagagaaagc catgagccgg cctatcctgt acagcaactg gctgtcaaag
    11341 gattatatcc cagttgacca agaagagcta agggattatg tcaaggctag gctgaaggtc
    11401 ttctatgaag aagaacttga tgttcccttg gtcttattca atgaagttct ggaccatgtg
    11461 ctgaggatcg acagaatatt ccgacaacct caaggccatc tgcttctgat tggtgttagt
    11521 ggagctggaa aaaccaccct gtctcgcttt gtcgcctgga tgaatggctt gagtgtatac
    11581 cagattaagg tccacaggaa gtacactggg gaagacttcg atgaagatct taggacagtg
    11641 ctgagacggt ctggctgtaa aaatgaaaag atagcattca taatggatga gtccaacgtc
    11701 ttagattccg gattcctgga acgaatgaat acccttctgg ctaatggcga ggttcctggg
    11761 ctctttgaag gggatgagta tgccacgctg atgacacagt gcaaagaggg ggcccagaag
    11821 gaggggctca tgctggactc ccacgaggag ctctacaagt ggttcaccag ccaggtgatc
    11881 cgcaacctgc acgtggtctt caccatgaac ccgtcctcag aggggctcaa ggacagggcg
    11941 gccacctcac cagccctttt caacaggtgt gtgctgaact ggtttggtga ctggtccact
    12001 gaagcactgt atcaagtcgg caaagaattc accagtaaga tggacctgga gaagccgaac
    12061 tatatagtgc ctgactacat gccagttgtg tatgataaac tgccgcagcc cccaacacac
    12121 cgggaagcca ttgtgaacag ctgtgtgttt gtccatcaga ctcttcacca ggctaatgcg
    12181 cgcctggcga agcgaggtgg cagaacgatg gccatcacac ctcggcacta cctggacttc
    12241 atcaaccact acgccaacct cttccatgag aagcgcagcg agttggagga gcagcagatg
    12301 cacctgaatg ttgggctccg aaaaatcaaa gagaccgtgg accaggtgga agaactgcgc
    12361 cgtgacctga ggatcaagag ccaagagctg gaggtcaaga acgcagcggc caatgacaag
    12421 ctgaagaaaa tggtgaaaga ccagcaagag gctgagaaga agaaggtcat gagccaagaa
    12481 atccaggagc agctacacaa acagcaggaa gtgatcgcag acaagcagat gagtgtcaag
    12541 gaggacctgg ataaagtgga gcctgctgtc atcgaggccc agaacgctgt gaagtccatc
    12601 aagaagcagc acctggtgga ggtgaggtcc atggccaacc ctcctgcagc tgtgaagctg
    12661 gctctggagt ccatctgcct gctgcttggt gagagcacca cggactggaa gcagatccgc
    12721 tccatcatca tgagagagaa cttcatcccc accatcgtca acttctcggc tgaggagatc
    12781 agtgatgcca tacgagagaa gatgaagaaa aactacatgt ccaaccccag ttacaactat
    12841 gagatcgtca accgggcttc cctggcttgt ggtcctatgg tgaagtgggc gattgcacag
    12901 ctcaattatg cagacatgtt aaagcgagtg gagcccctga ggaatgagct gcagaagctg
    12961 gaagatgacg ccaaggacaa ccagcagaaa gccaatgagg tggagcagat gatcagggac
    13021 ctggaagcca gcattgcccg ctacaaggag gagtacgctg tcctcatctc tgaggcccag
    13081 gccatcaagg cagacctggc agctgtggaa gcaaaggtaa accggagcac tgcacttcta
    13141 aagagtttat ctgccgaaag ggaacgttgg gaaaagacga gtgaaacttt caaaaaccag
    13201 atgtctacca ttgctgggga ctgcctcctg tcggcggcct tcattgctta cgctggctac
    13261 tttgaccagc aaatgcgcca gaacttgttc accacctggt ctcatcactt acagcaagcc
    13321 aacatccagt tccgcacaga cattgcaagg acggagtacc tctccaatgc tgacgaacgc
    13381 ctccgctggc aggccagctc tctgcctgcc gacgacctgt gcacagagaa tgccatcatg
    13441 ctaaaacgat tcaataggta cccgctgatc atcgacccct ccggacaggc cacagaattc
    13501 atcatgaatg aatataagga ccggaagatc acaaggacca gcttcttaga tgatgccttc
    13561 cggaagaatt tggagagtgc tctgagattt ggcaatcccc ttcttgtcca ggatgtggaa
    13621 agctacgatc cagttttgaa tccagtactg aaccgtgaag ttcggcgaac aggagggagg
    13681 gtgctgatta ccctcggtga ccaagacata gacctgtcac catcctttgt catctttctg
    13741 tctacccgag acccaactgt ggagttccca ccagacctct gctcccgggt gacctttgtg
    13801 aacttcaccg tcacccgcag cagtttacag agccagtgtc tcaatgaagt gcttaaagca
    13861 gaaaggccag acgtggacga gaagcgctcc gacctcctga aactgcaagg ggagttccaa
    13921 ctccgtttac ggcagcttga aaagtctctg ctccaagctc tgaatgaggt aaaaggccgc
    13981 attctggatg acgacaccat cattacaacc ctggagaatc tgaagcggga agctgccgag
    14041 gttaccagaa aggtggagga gacagatatt gtcatgcagg aggtggagac cgtgtcccag
    14101 cagtacctgc cgctgtccac tgcctgcagc agcatctact tcactatgga gtccttgaag
    14161 caggttcact tcctgtacca gtactctctg cagttcttcc tggacatcta ccacaacgtg
    14221 ctgtatgaga accccaacct gaagggtgcc accgatcaca cacagcgcct ctccatcata
    14281 accaaggacc tcttccaggt ggcctttaac cgagtggctc gaggcatgct gcaccaggac
    14341 cacatcacct tcgccatgct gctagcgagg atcaagctga aaggcaccgt gggggagccc
    14401 acctatgatg cagagttcca gcacttcctc agagggaagg agattgtgct gagtgctggc
    14461 tccaccccca aaatccaggg cctgaccgta gagcaggcag aagctgtcgt gaggttgagc
    14521 tgtctgcctg catttaaaga tctgattgcc aaggtgcagg cagatgagca gtttggcatt
    14581 tggctggata gcagttcacc agagcagacc gtgccatacc tctggagcga ggaaacgcct
    14641 acaactccca ttgggcaggc catccaccgc ctgctcctga ttcaagcctt ccgacccgac
    14701 cgcctgttgg ccatggccca catgtttgtt tccacaaacc tcggagagtc cttcatgtcc
    14761 atcatggagc agcctctcga cctcacccac attgtgggca cagaggtgaa gccaaacaca
    14821 cctgtcctga tgtgctctgt gcctgggtat gatgccagcg gacatgttga ggacctcgca
    14881 gcagagcaga acacgcagat cacgtctatt gccatcggct cagcagaagg ctttaaccaa
    14941 gcagataagg ccatcaacac cgccgtgaag tcaggcaggt gggtgatgtt gaagaacgtg
    15001 cacctggccc cgggctggct gatgcagctg gagaagaagc tgcactccct gcagccccac
    15061 gcctgcttcc gacttttcct caccatggag atcaacccca aggtgcccgt gaacctgctc
    15121 cgtgctggtc ggatctttgt gtttgagcca ccacccggag tgaaagccaa catgctgagg
    15181 acatttagca gcatccctgt ctctaggata tgcaagtctc caaatgagcg cgcccgcttg
    15241 tatttcctgc tggcttggtt ccatgccatc atccaagagc gcctgcgcta tgctccactg
    15301 gggtggtcaa agaagtacga atttggagaa tctgaccttc gatcagcttg tgacacggtg
    15361 gacacgtggc tggacgacac agccaagggc cggcagaaca tctcaccgga taagatccca
    15421 tggtctgccc tgaagaccct gatggcgcag tccatctatg gtgggcgggt ggacaatgag
    15481 ttcgaccagc gtctgctcaa caccttcctg gagcggctgt tcaccacccg cagcttcgac
    15541 agtgaattca agctggcgtg caaggttgac gggcacaagg acatccagat gcctgatggt
    15601 atcaggcgag aggagtttgt gcagtgggtg gagctgctgc ctgacgccca gacaccctca
    15661 tggctgggcc tccccaacaa tgctgaaagg gtcctgctta ccacccaagg cgtggacatg
    15721 atcagcaaga tgctgaagat gcagatgctg gaggacgagg atgacctggc ctacgcagag
    15781 acggagaaga aggcaaggac ggactccacc tccgatggcc gtccagcctg gatgaggaca
    15841 ctgcacacca cggcgtccaa ctggctgcac ctcatcccac agacactgag ccccctgaag
    15901 cgcaccgtgg agaacatcaa ggatcctttg tttaggttct ttgagagaga agtaaagatg
    15961 ggggccaagt tactccaaga tgtgcgccaa gatcttgctg acgtcgttca agtgtgcgag
    16021 gggaaaaaga agcagacaaa ctacctgcgc acgctcatca atgagctggt gaaagggatc
    16081 ttgcctcgga gctggtccca ttacacagtg cctgcaggca tgactgtgat ccagtgggtg
    16141 tcagatttca gcgagaggat caaacagctg cagaacatct cacaagcagc tgcatctggt
    16201 ggtgccaagg aactgaagaa catccacgtg tgcctgggtg gcctgtttgt acctgaggcc
    16261 tacatcactg ccaccagaca gtatgtggca caagctaaca gctggtccct ggaggaactc
    16321 tgcctggaag tcaatgtcac cgcctcacag agtgccaccc ttgatgcctg cagctttgga
    16381 gtcacaggtc tgaagctcca aggggccacg tgcagcaaca acaagctatc actctccaat
    16441 gccatctcca cagtcctccc cctcacacag ctgcgctggg tgaaacaaac aagcgccgag
    16501 aagaaggcca gcgtggtgac cttacctgtc tacctgaact tcacccgggc agacctcatc
    16561 ttcaccgtgg actttgaaat tgctacaaag gaagatcctc gcagcttcta tgagcgtgga
    16621 gttgccgttc tgtgcactga gtaaaagctt cgaattctgc agtcg
//
