LOCUS       sequence-381753-       15933 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the dynein motor domain fused to the dimerization domain 
            FKBP to recruit it to the plasma membrane by using the chemical 
            dimerization system FKBP-FRB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15933)
  AUTHORS   Schelski M, Bradke F
  TITLE     Microtubule retrograde flow retains neuronal polarization in a 
            fluctuating state.
  JOURNAL   Sci Adv. 2022 Nov 4;8(44):eabo2336. doi: 10.1126/sciadv.abo2336. 
            Epub 2022 Nov 4.
  PUBMED    36332023
REFERENCE   2  (bases 1 to 15933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2022-11-4"; volume: "8(44):eabo2336. doi"; pages: " 
            10.1126/sciadv.abo2336. Epub 2022 Nov 4"
COMMENT     Sequence Label: pBetaActin-FKBP-Dync1h1motor
FEATURES             Location/Qualifiers
     source          1..15933
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    150..284
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(187..206)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     241..260
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(298..314)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(298..314)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(311..333)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    322..338
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(346..352,353..370,371..376))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    391..412
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(529..546)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(700..1288)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(780..799)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1459..2250,2251..2319))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2082..2101
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2320..2424)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2492..2510
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2548..2570)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2670..2689
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2883..2905
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2897..2914
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2898..2914
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4715..4990
                     /label=chicken beta-actin promoter
     primer_bind     5915..5937
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             join(5989..5991,5992..6312)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     regulatory      10282..10291
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 acggtaccgc gggcccggga tccaccggat ctagataagt aatgatcata atcagccata
       61 tcacatctgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
      121 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
      181 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
      241 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaattcg taatcatgtc
      301 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
      361 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
      421 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
      481 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
      541 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
      601 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
      661 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
      721 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
      781 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
      841 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
      901 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
      961 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     1021 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     1081 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     1141 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     1201 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     1261 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     1321 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     1381 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     1441 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     1501 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     1561 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     1621 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     1681 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     1741 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     1801 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     1861 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     1921 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     1981 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     2041 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     2101 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     2161 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     2221 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     2281 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     2341 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     2401 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     2461 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     2521 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     2581 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     2641 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     2701 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
     2761 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     2821 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     2881 ttcccagtca cgacgttgta aaacgacggc cagtgccaag ttgggatctt tgcattggcc
     2941 cacggctctc aggatgggga tgctcccctt cagcacccgg ttccccttgg aaactgatgg
     3001 tcctggctct gtggcatggc agtggcactg tgaggagccc cctaccagca gcacacagtg
     3061 gggttggcac tgccacgctc cggatgccgc gctctgatcc aaccccataa tcaagggaac
     3121 ccgaattgcc ccatcattgc ccccaccacc cccatcctgc cgggccctca caccccacgc
     3181 tgccttctgg tgacattccc cagcccaaac ccacggcttc atggctaccg cggggcattt
     3241 cccattgccg ccccattatc agctctgcac acctcccgct gtacccatgc ctcgtggctg
     3301 cccttctttg acgtataatc ttctaattaa tacccggcct tgtcaaagtg gagcacaaac
     3361 gttaattaat tccccagcag gcaggtaatt aacagtgtga ctcccttttt gctgcgagtg
     3421 gggctgatac agagagatgt ggcactatgg agcccacggg gtcctggcac tgggtgccca
     3481 cggaggtccc catgtgctgc agtgtcaccg cctccgagga gacagtattg tccctgcggt
     3541 gtccctgcag ctcagctctg tccacagggc cacctccagt ttggagggga cacaatgcag
     3601 ccccgatgca acccatcctc gcagcatccc agggacaaag accccactgc aagaccgcac
     3661 acagggctgg gtcccgctcc cctaatatct acagtgcttc ttgcatggcc ccttaatcaa
     3721 tgcagttaat cagcatgcgc tcatgcaccg ctctggagct gcaaagcccc tcgcagcgct
     3781 gctcaccaac accgcgcacc gccccggccc agcctgcagc acgcgctgca aacaggaaag
     3841 aaacaaaata ttgcccaaat gtaggcaaag gcattcggct gccttgacct ccgccgggcc
     3901 gggccctgcc tgactcagct ccttactcag cgctcgcttc ctccctccgg ctgccaccgc
     3961 cgcagcgcac accctgacaa agagtggccc ttaacgggct ctgaggtgca cccagcagtg
     4021 cactcagcag tccaagggcc ggcctggagg tttgcaccgc tacgtgctga cattagcatt
     4081 gaacttggcc ctgggtagtg ctgcaggccg ggcggggtgg gtgtagagag tgcagcgcgc
     4141 gttgcacccg gtgccccttc ccctcccttg catcccagca ggctgcaccc cagcaccagg
     4201 cccgtgcatg catgctcctg gtgttattgc agcctggtgc atgcatgcgt cttgatggtg
     4261 cagcgctgtg catgcatcct ccttggtgtg tagcagtcta gtgcatgcat acccctcggt
     4321 gttattgctg ctctgtgcac gcacgctcat tgtatcactt catcccagtg catgcactca
     4381 cactggagcg attgctgctc ggtgcacgca cactcattgt atcactgcag ctcagtggct
     4441 gcacgcacac cggtgttatt gctgctcggt gcgtgcatgc acatcagtgt cgctgcagct
     4501 cagtgcatgc acgctcattg cccatcgcta tccctgcctc tcctgctggc gctccccggg
     4561 aggtgacttc aaggggaccg caggaccacc tcgggggtgg ggggagggct gcacacgcgg
     4621 accccgctcc ccctccccaa caaagcactg tggaatcaaa aaggggggag gggggatgga
     4681 ggggcgcgtc acacccccgc cccacaccct cacctcgagg tgagccccac gttctgcttc
     4741 actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta
     4801 ttttgtgcag cgatgggggc gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg
     4861 cgrggggcgg ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct
     4921 ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc
     4981 gcggcgggcg ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc
     5041 gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg
     5101 cccttctcct ccgggctgta attagcgctt ggtttaatga cggctcgttt cttttctgtg
     5161 gctgcgtgaa agccttaaag ggctccggga gggccctttg tgcggggggg agcggctcgg
     5221 ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg
     5281 gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga
     5341 gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg
     5401 tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg cgcggcggtc gggctgtaac
     5461 ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc
     5521 cgtgcggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggkgggggtg
     5581 ccgggcgggg cggggccgcc tcgggccggg grgggctcgg gggrggggcg cggcggctgt
     5641 cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga
     5701 cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct
     5761 agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     5821 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg tccgcagggg
     5881 gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc
     5941 ggggtttata tcttcccttc tctgttcctc cgcagccccc aagctagcat gggagtgcag
     6001 gtggaaacca tctccccagg agacgggcgc accttcccca agcgcggcca gacctgcgtg
     6061 gtgcactaca ccgggatgct tgaagatgga aagaaatttg attcctcccg ggacagaaac
     6121 aagcccttta agtttatgct aggcaagcag gaggtgatcc gaggctggga agaaggggtt
     6181 gcccagatga gtgtgggtca gagagccaaa ctgactatat ctccagatta tgcctatggt
     6241 gccactgggc acccaggcat catcccacca catgccactc tcgtcttcga tgtggagctt
     6301 ctaaaactgg aatctggtgg atcaagtggt ggaggcgaga tggccttgga agaatttttg
     6361 aagcagatac gagaagtgtg gaacacatac gaactagact tggtcaacta tcagaacaaa
     6421 tgccgcctca tccgaggctg ggatgacctc ttcaacaagg tcaaggagca tatcaacagc
     6481 gtctctgcca tgaaactctc tccctattac aaggtttttg aagaagatgc cctgagctgg
     6541 gaggacaagc tgaaccgaat catggctctc tttgacgtgt ggattgacgt gcagaggcgc
     6601 tgggtctact tggaaggcat cttcacaggc agtgcggaca tcaaacatct gctgcctgtg
     6661 gaaacccagc gcttccagag tatcagcacg gagtttttgg ctctgatgaa aaaagtctcc
     6721 aagtctccac ttgtgatgga tgttctgaac atacagggag tccagaggtc tctggaaagg
     6781 ttggcagacc tgctggggaa gatccagaaa gcattggggg agtacctgga acgggagcgc
     6841 tcgtctttcc ccaggttcta ctttgtgggt gatgaggatt tgcttgagat cattgggaat
     6901 agcaagaacg tggctaagtt gcagaagcac ttcaagaaaa tgttcgccgg cgtttccagc
     6961 atcatcttaa atgaggataa ctctgtggtc ctcggcatct catcccgaga aggggaggag
     7021 gttatgttta aaactccagt gtccatcact gaacacccca aaatcaatga gtggctcact
     7081 ctggtggaaa aggagatgag agtcaccttg gctaaacttc tggcagagtc tgttacagaa
     7141 gtggagattt ttggaaaagc aacttctatt gacccaaata cctacattac ttggattgat
     7201 aaataccagg cccagctagt ggtattgtca gcccagatag cctggtccga gaacgtggag
     7261 aatgcgctga gcaacgttgg gggaggtggt gatgtggggc ccttgcagtc tgtgctgagc
     7321 aatgtggagg tcacactcaa cgtcttagcg gactctgtcc tcatggagca gcctcctctc
     7381 cggagacgga agctggagca cttgatcaca gagctggttc accaaagaga cgttaccagg
     7441 tccttgatca aaagcaagat cgacaacgcc aaatctttcg aatggctcag ccagatgcga
     7501 ttttactttg accctaagca aacggacgtg ttacagcagc tgtccattca gatggccaat
     7561 gccaagttta actacggctt tgagtacctg ggtgttcaag acaagctggt tcagacgccc
     7621 ctcactgacc gttgctattt gacaatgaca caagctttgg aggccaggct tgggggctcc
     7681 ccatttggac ctgctggaac cggaaagaca gagtccgtca aagccctcgg ccatcagctg
     7741 gggcggttcg tcttagtttt caactgtgac gaaacctttg atttccaggc gatgggacgt
     7801 atctttgtgg ggctttgcca agtgggcgcg tggggctgct ttgatgagtt taaccgcctg
     7861 gaggagcgga tgctctcagc tgtatcccag caggtgcagt gcatacaaga ggcactgcgg
     7921 gagcattcca accccaacta cgacaagacc tcagccccca ttacttgtga gctgctgaac
     7981 aaacaagtca aggttagccc agacatggct atcttcatca ccatgaaccc aggctatgca
     8041 ggtcgctcaa acctccccga caacctcaag aagctcttcc ggagcttggc gatgaccaag
     8101 cctgaccggc agctgatcgc ccaggtcatg ctgtactcgc agggcttccg gactgctgag
     8161 gtccttgcca acaagatcgt cccgttcttc aaactgtgtg atgagcaact gtcttctcaa
     8221 agccattatg actttgggct tcgggctttg aagagtgtgc ttgtaagtgc aggcaatgtg
     8281 aagagggaga ggatccagaa gataaagagg gagaaagaag aacgggggga agcagttgat
     8341 gaaggagaga ttgctgagaa cctacctgag caggagattc tgatccagag tgtctgcgag
     8401 acaatggtgc ccaagctggt ggcagaagat atcccgctgc tcttcagcct cctgtctgac
     8461 gtgtttcctg gagtccagta ccaccgtggg gagatgacag cccttcgaga agagctgaag
     8521 aaagtgtgtc aggagatgta cttgacatat ggcgacggag aagaagttgg cgggatgtgg
     8581 gttgaaaagg ttctccagct ctatcagatc acgcagatta accacggcct gatgatggtt
     8641 gggccttcag gaagtgggaa gagcatggcc tggcgagtgc tgctgaaggc tctagagagg
     8701 cttgagggtg tggagggcgt ggcccacata attgacccca aggccatcag caaagaccac
     8761 ctctacggaa ccttggatcc caacaccagg gagtggacag acggactctt cacacacgtg
     8821 ctgagaaaga tcatagacaa tgtgagaggt gagctgcaga aacgccagtg gattgtcttt
     8881 gatggcgatg tggaccccga gtgggtggag aacctgaact ccgtgctgga tgacaacaag
     8941 ctcctgacgc tgcctaatgg agagcgcctt agcctgcctc ccaatgtgcg aataatgttt
     9001 gaagtgcagg acttgaaata cgcgacgctg gccacggtgt ctcgctgtgg catggtgtgg
     9061 ttcagtgagg acgtcctgag cacagacatg atcttcaata acttcctggc caggctgcgc
     9121 agcattcccc tggatgaagg ggaggacgag gcacagcgta ggcgaaaagg caaagaagac
     9181 gagggcgaag aggccgcttc tccaatgctg cagattcaaa gagatgcagc aacgatcatg
     9241 cagccgtact tcacgtccaa cggcctggtc accaaggcgc tggagcacgc cttcaagctg
     9301 gagcacatca tggacctgac gcgcctgcgc tgcctgggct cgctcttctc catgctgcac
     9361 caggcctgcc gcaacgtggc gcagtacaat gccaaccatc ctgatttccc catgcagatt
     9421 gagcagctgg agcgctacat tcagcgctat ctggtttacg ccatcctctg gtccttatct
     9481 ggagacagcc ggctgaagat gagagcagag ctgggcgagt acatccggag gatcaccact
     9541 gtgccgctgc ccactgcgcc caacgtcccc atcattgact atgaggtgtc catcagtgga
     9601 gagtggtcgc cgtggcaggc caaggtgcct cagattgaag tggagacaca caaggtggca
     9661 gccccagatg tcgtcgtgcc aaccttagat acagtccgcc atgaagctct cctgtacact
     9721 tggctggctg aacacaaacc cctggtctta tgtggccctc ctgggtctgg caagaccatg
     9781 acactcttca gtgctctccg ggccttgcct gacatggagg ttgtgggcct aaacttctca
     9841 agtgccacta caccagagct gttgctgaag acgtttgacc actactgcga atacaggcgt
     9901 acacctaacg gtgttgtctt ggctcctgtt cagcttggaa agtggctggt cttgttctgt
     9961 gatgaaatca acttgcccga tatggataaa tacggcacac agagagtcat ctccttcatc
    10021 agacagatgg tggagcatgg aggcttctat cgtacctcag accaaacgtg ggtaaagcta
    10081 gagagaatcc agtttgttgg ggcttgcaat ccacccactg accctgggag aaagcccctc
    10141 tcacacaggt tcttgcgcca cgtgcctgtt gtatatgtgg attacccagg accagcctcc
    10201 ctgacgcaaa tctatggcac ttttaaccgc gccatgctga ggctcattcc ctcgctccgt
    10261 acctatgcag aacccctcac tgccgccatg gtggagttct ataccatgtc acaggagcgg
    10321 tttactcagg acacacagcc acactatatc tactcgcccc gtgagatgac tcggtgggta
    10381 agaggaatct ttgaagcact gagacccctg gagacattgc ctgttgaagg tctcattcgt
    10441 atttgggcac atgaagccct ccgtctcttc caagacaggc ttgtggagga tgaggagcgg
    10501 cgctggactg acgaaaacat tgacatggtt gccttgaagc acttccctaa catagacaaa
    10561 gagaaagcca tgagccggcc tatcctgtac agcaactggc tgtcaaagga ttatatccca
    10621 gttgaccaag aagagctaag ggattatgtc aaggctaggc tgaaggtctt ctatgaagaa
    10681 gaacttgatg ttcccttggt cttattcaat gaagttctgg accatgtgct gaggatcgac
    10741 agaatattcc gacaacctca aggccatctg cttctgattg gtgttagtgg agctggaaaa
    10801 accaccctgt ctcgctttgt cgcctggatg aatggcttga gtgtatacca gattaaggtc
    10861 cacaggaagt acactgggga agacttcgat gaagatctta ggacagtgct gagacggtct
    10921 ggctgtaaaa atgaaaagat agcattcata atggatgagt ccaacgtctt agattccgga
    10981 ttcctggaac gaatgaatac ccttctggct aatggcgagg ttcctgggct ctttgaaggg
    11041 gatgagtatg ccacgctgat gacacagtgc aaagaggggg cccagaagga ggggctcatg
    11101 ctggactccc acgaggagcc ctacaagtgg ttcaccagcc aggtgatccg caacctgcac
    11161 gtggtcttca ccatgaaccc gtcctcagag gggctcaagg acagggcggc cacctcacca
    11221 gcccttttca acaggtgtgt gctgaactgg tttggtgact ggtccactga agcactgtat
    11281 caagtcggca aagaattcac cagtaagatg gacctggaga agccgaacta tatagtgcct
    11341 gactacatgc cagttgtgta tgataaactg ccgcagcccc caacacaccg ggaagccatt
    11401 gtgaacagct gtgtgtttgt ccatcagact cttcaccagg ctaatgcgcg cctggcgaag
    11461 cgaggtggca gaacgatggc catcacacct cggcactacc tggacttcat caaccactac
    11521 gccaacctct tccatgagaa gcgcagcgag ttggaggagc agcagatgca cctgaatgtt
    11581 gggctccgaa aaatcaaaga gaccgtggac caggtggaag aactgcgccg tgacctgagg
    11641 atcaagagcc aagagctgga ggtcaagaac gcagcggcca atgacaagct gaagaaaatg
    11701 gtgaaagacc agcaagaggc tgagaagaag aaggtcatga gccaagaaat ccaggagcag
    11761 ctacacaaac agcaggaagt gatcgcagac aagcagatga gtgtcaagga ggacctggat
    11821 aaagtggagc ctgctgtcat cgaggcccag aacgctgtga agtccatcaa gaagcagcac
    11881 ctggtggagg tgaggtccat ggccaaccct cctgcagctg tgaagctggc tctggagtcc
    11941 atctgcctgc tgcttggtga gagcaccacg gactggaagc agatccgctc catcatcatg
    12001 agagagaact tcatccccac catcgtcaac ttctcggctg aggagatcag tgatgccata
    12061 cgagagaaga tgaagaaaaa ctacatgtcc aaccccagtt acaactatga gatcgtcaac
    12121 cgggcttccc tggcttgtgg tcctatggtg aagtgggcga ttgcacagct caattatgca
    12181 gacatgttaa agcgagtgga gcccctgagg aatgagctgc agaagctgga agatgacgcc
    12241 aaggacaacc agcagaaagc caatgaggtg gagcagatga tcagggacct ggaagccagc
    12301 attgcccgct acaaggagga gtacgctgtc ctcatctctg aggcccaggc catcaaggca
    12361 gacctggcag ctgtggaagc aaaggtaaac cggagcactg cacttctaaa gagtttatct
    12421 gccgaaaggg aacgttggga aaagacgagt gaaactttca aaaaccagat gtctaccatt
    12481 gctggggact gcctcctgtc ggcggccttc attgcttacg ctggctactt tgaccagcaa
    12541 atgcgccaga acttgttcac cacctggtct catcacttac agcaagccaa catccagttc
    12601 cgcacagaca ttgcaaggac ggagtacctc tccaatgctg acgaacgcct ccgctggcag
    12661 gccagctctc tgcctgccga cgacctgtgc acagagaatg ccatcatgct aaaacgattc
    12721 aataggtacc cgctgatcat cgacccctcc ggacaggcca cagaattcat catgaatgaa
    12781 tataaggacc ggaagatcac aaggaccagc ttcttagatg atgccttccg gaagaatttg
    12841 gagagtgctc tgagatttgg caatcccctt cttgtccagg atgtggaaag ctacgatcca
    12901 gttttgaatc cagtactgaa ccgtgaagtt cggcgaacag gagggagggt gctgattacc
    12961 ctcggtgacc aagacataga cctgtcacca tcctttgtca tctttctgtc tacccgagac
    13021 ccaactgtgg agttcccacc agacctctgc tcccgggtga cctttgtgaa cttcaccgtc
    13081 acccgcagca gtttacagag ccagtgtctc aatgaagtgc ttaaagcaga aaggccagac
    13141 gtggacgaga agcgctccga cctcctgaaa ctgcaagggg agttccaact ccgtttacgg
    13201 cagcttgaaa agtctctgct ccaagctctg aatgaggtaa aaggccgcat tctggatgac
    13261 gacaccatca ttacaaccct ggagaatctg aagcgggaag ctgccgaggt taccagaaag
    13321 gtggaggaga cagatattgt catgcaggag gtggagaccg tgtcccagca gtacctgccg
    13381 ctgtccactg cctgcagcag catctacttc actatggagt ccttgaagca ggttcacttc
    13441 ctgtaccagt actctctgca gttcttcctg gacatctacc acaacgtgct gtatgagaac
    13501 cccaacctga agggtgccac cgatcacaca cagcgcctct ccatcataac caaggacctc
    13561 ttccaggtgg cctttaaccg agtggctcga ggcatgctgc accaggacca catcaccttc
    13621 gccatgctgc tagcgaggat caagctgaaa ggcaccgtgg gggagcccac ctatgatgca
    13681 gagttccagc acttcctcag agggaaggag attgtgctga gtgctggctc cacccccaaa
    13741 atccagggcc tgaccgtaga gcaggcagaa gctgtcgtga ggttgagctg tctgcctgca
    13801 tttaaagatc tgattgccaa ggtgcaggca gatgagcagt ttggcatttg gctggatagc
    13861 agttcaccag agcagaccgt gccatacctc tggagcgagg aaacgcctac aactcccatt
    13921 gggcaggcca tccaccgcct gctcctgatt caagccttcc gacccgaccg cctgttggcc
    13981 atggcccaca tgtttgtttc cacaaacctc ggagagtcct tcatgtccat catggagcag
    14041 cctctcgacc tcacccacat tgtgggcaca gaggtgaagc caaacacacc tgtcctgatg
    14101 tgctctgtgc ctgggtatga tgccagcgga catgttgagg acctcgcagc agagcagaac
    14161 acgcagatca cgtctattgc catcggctca gcagaaggct ttaaccaagc agataaggcc
    14221 atcaacaccg ccgtgaagtc aggcaggtgg gtgatgttga agaacgtgca cctggccccg
    14281 ggctggctga tgcagctgga gaagaagctg cactccctgc agccccacgc ctgcttccga
    14341 cttttcctca ccatggagat caaccccaag gtgcccgtga acctgctccg tgctggtcgg
    14401 atctttgtgt ttgagccacc acccggagtg aaagccaaca tgctgaggac atttagcagc
    14461 atccctgtct ctaggatatg caagtctcca aatgagcgcg cccgcttgta tttcctgctg
    14521 gcttggttcc atgccatcat ccaagagcgc ctgcgctatg ctccactggg gtggtcaaag
    14581 aagtacgaat ttggagaatc tgaccttcga tcagcttgtg acacggtgga cacgtggctg
    14641 gacgacacag ccaagggccg gcagaacatc tcaccggata agatcccatg gtctgccctg
    14701 aagaccctga tggcgcagtc catctatggt gggcgggtgg acaatgagtt cgaccagcgt
    14761 ctgctcaaca ccttcctgga gcggctgttc accacccgca gcttcgacag tgaattcaag
    14821 ctggcgtgca aggttgacgg gcacaaggac atccagatgc ctgatggtat caggcgagag
    14881 gagtttgtgc agtgggtgga gctgctgcct gacgcccaga caccctcatg gctgggcctc
    14941 cccaacaatg ctgaaagggt cctgcttacc acccaaggcg tggacatgat cagcaagatg
    15001 ctgaagatgc agatgctgga ggacgaggat gacctggcct acgcagagac ggagaagaag
    15061 gcaaggacgg actccacctc cgatggccgt ccagcctgga tgaggacact gcacaccacg
    15121 gcgtccaact ggctgcacct catcccacag acactgagcc ccctgaagcg caccgtggag
    15181 aacatcaagg atcctttgtt taggttcttt gagagagaag taaagatggg ggccaagtta
    15241 ctccaagatg tgcgccaaga tcttgctgac gtcgttcaag tgtgcgaggg gaaaaagaag
    15301 cagacaaact acctgcgcac gctcatcaat gagctggtga aagggatctt gcctcggagc
    15361 tggtcccatt acacagtgcc tgcaggcatg actgtgatcc agtgggtgtc agatttcagc
    15421 gagaggatca aacagctgca gaacatctca caagcagctg catctggtgg tgccaaggaa
    15481 ctgaagaaca tccacgtgtg cctgggtggc ctgtttgtac ctgaggccta catcactgcc
    15541 accagacagt atgtggcaca agctaacagc tggtccctgg aggaactctg cctggaagtc
    15601 aatgtcaccg cctcacagag tgccaccctt gatgcctgca gctttggagt cacaggtctg
    15661 aagctccaag gggccacgtg cagcaacaac aagctatcac tctccaatgc catctccaca
    15721 gtcctccccc tcacacagct gcgctgggtg aaacaaacaa gcgccgagaa gaaggccagc
    15781 gtggtgacct tacctgtcta cctgaacttc acccgggcag acctcatctt caccgtggac
    15841 tttgaaattg ctacaaagga agatcctcgc agcttctatg agcgtggagt tgccgttctg
    15901 tgcactgagt aaaagcttcg aattctgcag tcg
//
