LOCUS       sequence-381754-       16810 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the fluorescently labeled dynein motor domain fused to the
            dimerization domain FKBP to recruit it to the plasma membrane by 
            using the chemical dimerization system FKBP-FRB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16810)
  AUTHORS   Schelski M, Bradke F
  TITLE     Microtubule retrograde flow retains neuronal polarization in a 
            fluctuating state.
  JOURNAL   Sci Adv. 2022 Nov 4;8(44):eabo2336. doi: 10.1126/sciadv.abo2336. 
            Epub 2022 Nov 4.
  PUBMED    36332023
REFERENCE   2  (bases 1 to 16810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2022-11-4"; volume: "8(44):eabo2336. doi"; pages: " 
            10.1126/sciadv.abo2336. Epub 2022 Nov 4"
COMMENT     Sequence Label: pBetaActin-FKBP-Halo-Dync1h1motor
FEATURES             Location/Qualifiers
     source          1..16810
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    101..235
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(138..157)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     192..211
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(249..265)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(249..265)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(262..284)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    273..289
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(297..303,304..321,322..327))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    342..363
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(480..497)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(651..1239)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(731..750)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1410..2201,2202..2270))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2033..2052
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2271..2375)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2443..2461
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2499..2521)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2621..2640
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2834..2856
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2848..2865
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2849..2865
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5831..5853
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             join(5905..5907,5908..6228)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             6250..7140
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     regulatory      11110..11119
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aatcagccat atcacatctg tagaggtttt acttgcttta aaaaacctcc cacacctccc
       61 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
      121 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
      181 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaattc
      241 gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
      301 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
      361 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
      421 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
      481 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
      541 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
      601 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
      661 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
      721 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
      781 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
      841 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
      901 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
      961 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1021 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1081 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     1141 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1201 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     1261 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     1321 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     1381 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     1441 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     1501 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     1561 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     1621 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     1681 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     1741 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     1801 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     1861 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     1921 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     1981 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     2041 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     2101 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     2161 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     2221 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     2281 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     2341 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     2401 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     2461 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     2521 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     2581 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     2641 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     2701 atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     2761 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     2821 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gttgggatct
     2881 ttgcattggc ccacggctct caggatgggg atgctcccct tcagcacccg gttccccttg
     2941 gaaactgatg gtcctggctc tgtggcatgg cagtggcact gtgaggagcc ccctaccagc
     3001 agcacacagt ggggttggca ctgccacgct ccggatgccg cgctctgatc caaccccata
     3061 atcaagggaa cccgaattgc cccatcattg cccccaccac ccccatcctg ccgggccctc
     3121 acaccccacg ctgccttctg gtgacattcc ccagcccaaa cccacggctt catggctacc
     3181 gcggggcatt tcccattgcc gccccattat cagctctgca cacctcccgc tgtacccatg
     3241 cctcgtggct gcccttcttt gacgtataat cttctaatta atacccggcc ttgtcaaagt
     3301 ggagcacaaa cgttaattaa ttccccagca ggcaggtaat taacagtgtg actccctttt
     3361 tgctgcgagt ggggctgata cagagagatg tggcactatg gagcccacgg ggtcctggca
     3421 ctgggtgccc acggaggtcc ccatgtgctg cagtgtcacc gcctccgagg agacagtatt
     3481 gtccctgcgg tgtccctgca gctcagctct gtccacaggg ccacctccag tttggagggg
     3541 acacaatgca gccccgatgc aacccatcct cgcagcatcc cagggacaaa gaccccactg
     3601 caagaccgca cacagggctg ggtcccgctc ccctaatatc tacagtgctt cttgcatggc
     3661 cccttaatca atgcagttaa tcagcatgcg ctcatgcacc gctctggagc tgcaaagccc
     3721 ctcgcagcgc tgctcaccaa caccgcgcac cgccccggcc cagcctgcag cacgcgctgc
     3781 aaacaggaaa gaaacaaaat attgcccaaa tgtaggcaaa ggcattcggc tgccttgacc
     3841 tccgccgggc cgggccctgc ctgactcagc tccttactca gcgctcgctt cctccctccg
     3901 gctgccaccg ccgcagcgca caccctgaca aagagtggcc cttaacgggc tctgaggtgc
     3961 acccagcagt gcactcagca gtccaagggc cggcctggag gtttgcaccg ctacgtgctg
     4021 acattagcat tgaacttggc cctgggtagt gctgcaggcc gggcggggtg ggtgtagaga
     4081 gtgcagcgcg cgttgcaccc ggtgcccctt cccctccctt gcatcccagc aggctgcacc
     4141 ccagcaccag gcccgtgcat gcatgctcct ggtgttattg cagcctggtg catgcatgcg
     4201 tcttgatggt gcagcgctgt gcatgcatcc tccttggtgt gtagcagtct agtgcatgca
     4261 tacccctcgg tgttattgct gctctgtgca cgcacgctca ttgtatcact tcatcccagt
     4321 gcatgcactc acactggagc gattgctgct cggtgcacgc acactcattg tatcactgca
     4381 gctcagtggc tgcacgcaca ccggtgttat tgctgctcgg tgcgtgcatg cacatcagtg
     4441 tcgctgcagc tcagtgcatg cacgctcatt gcccatcgct atccctgcct ctcctgctgg
     4501 cgctccccgg gaggtgactt caaggggacc gcaggaccac ctcgggggtg gggggagggc
     4561 tgcacacgcg gaccccgctc cccctcccca acaaagcact gtggaatcaa aaagggggga
     4621 ggggggatgg aggggcgcgt cacacccccg ccccacaccc tcacctcgag gtgagcccca
     4681 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
     4741 ttttttaatt attttgtgca gcgatggggg cggggsgagg cggagaggtg cggcggcagc
     4801 caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc
     4861 ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc
     4921 ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca
     4981 ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg
     5041 gctcgtttct tttctgtggc tgcgtgaaag ccttaaaggg ctccgggagg gccctttgtg
     5101 cgggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg
     5161 gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc
     5221 gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg
     5281 aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg
     5341 cggcggtcgg gctgtaaccc ccccctgcac ccccctcccc gagttgctga gcacggcccg
     5401 gcttcgggtg cggggctccg tgcggggcgt ggcgcggggc tcgccgtgcc gggcgggggg
     5461 tggcggcagg tgggggtgcc gggcggggcg gggccgcctc gggccgggga gggctcgggg
     5521 gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat
     5581 tgccttttat ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg
     5641 gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc
     5701 ggcgccggca ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc
     5761 cttctccatc tccagcctcg gggctgtccg cagggggacg gctgccttcg ggggggacgg
     5821 ggcagggcgg ggttcggctt ctggcgtgtg accggcgggg tttatatctt cccttctctg
     5881 ttcctccgca gcccccaagc tagcatggga gtgcaggtgg aaaccatctc cccaggagac
     5941 gggcgcacct tccccaagcg cggccagacc tgcgtggtgc actacaccgg gatgcttgaa
     6001 gatggaaaga aatttgattc ctcccgggac agaaacaagc cctttaagtt tatgctaggc
     6061 aagcaggagg tgatccgagg ctgggaagaa ggggttgccc agatgagtgt gggtcagaga
     6121 gccaaactga ctatatctcc agattatgcc tatggtgcca ctgggcaccc aggcatcatc
     6181 ccaccacatg ccactctcgt cttcgatgtg gagcttctaa aactggaagg aagttcagga
     6241 ggttctagta tggcagaaat cggtactggc tttccattcg acccccatta tgtggaagtc
     6301 ctgggcgagc gcatgcacta cgtcgatgtt ggtccgcgcg atggcacccc tgtgctgttc
     6361 ctgcacggta acccgacctc ctcctacgtg tggcgcaaca tcatcccgca tgttgcaccg
     6421 acccatcgct gcattgctcc agacctgatc ggtatgggca aatccgacaa accagacctg
     6481 ggttatttct tcgacgacca cgtccgcttc atggatgcct tcatcgaagc cctgggtctg
     6541 gaagaggtcg tcctggtcat tcacgactgg ggctccgctc tgggtttcca ctgggccaag
     6601 cgcaatccag agcgcgtcaa aggtattgca tttatggagt tcatccgccc tatcccgacc
     6661 tgggacgaat ggccagaatt tgcccgcgag accttccagg ccttccgcac caccgacgtc
     6721 ggccgcaagc tgatcatcga tcagaacgtt tttatcgagg gtacgctgcc gatgggtgtc
     6781 gtccgcccgc tgactgaagt cgagatggac cattaccgcg agccgttcct gaatcctgtt
     6841 gaccgcgagc cactgtggcg cttcccaaac gagctgccaa tcgccggtga gccagcgaac
     6901 atcgtcgcgc tggtcgaaga atacatggac tggctgcacc agtcccctgt cccgaagctg
     6961 ctgttctggg gcaccccagg cgttctgatc ccaccggccg aagccgctcg cctggccaaa
     7021 agcctgccta actgcaaggc tgtggacatc ggcccgggtc tgaatctgct gcaagaagac
     7081 aacccggacc tgatcggcag cgagatcgcg cgctggctgt cgacgctcga gatttccggc
     7141 tctggtggat caagtggtgg aggcgagatg gccttggaag aatttttgaa gcagatacga
     7201 gaagtgtgga acacatacga actagacttg gtcaactatc agaacaaatg ccgcctcatc
     7261 cgaggctggg atgacctctt caacaaggtc aaggagcata tcaacagcgt ctctgccatg
     7321 aaactctctc cctattacaa ggtttttgaa gaagatgccc tgagctggga ggacaagctg
     7381 aaccgaatca tggctctctt tgacgtgtgg attgacgtgc agaggcgctg ggtctacttg
     7441 gaaggcatct tcacaggcag tgcggacatc aaacatctgc tgcctgtgga aacccagcgc
     7501 ttccagagta tcagcacgga gtttttggct ctgatgaaaa aagtctccaa gtctccactt
     7561 gtgatggatg ttctgaacat acagggagtc cagaggtctc tggaaaggtt ggcagacctg
     7621 ctggggaaga tccagaaagc attgggggag tacctggaac gggagcgctc gtctttcccc
     7681 aggttctact ttgtgggtga tgaggatttg cttgagatca ttgggaatag caagaacgtg
     7741 gctaagttgc agaagcactt caagaaaatg ttcgccggcg tttccagcat catcttaaat
     7801 gaggataact ctgtggtcct cggcatctca tcccgagaag gggaggaggt tatgtttaaa
     7861 actccagtgt ccatcactga acaccccaaa atcaatgagt ggctcactct ggtggaaaag
     7921 gagatgagag tcaccttggc taaacttctg gcagagtctg ttacagaagt ggagattttt
     7981 ggaaaagcaa cttctattga cccaaatacc tacattactt ggattgataa ataccaggcc
     8041 cagctagtgg tattgtcagc ccagatagcc tggtccgaga acgtggagaa tgcgctgagc
     8101 aacgttgggg gaggtggtga tgtggggccc ttgcagtctg tgctgagcaa tgtggaggtc
     8161 acactcaacg tcttagcgga ctctgtcctc atggagcagc ctcctctccg gagacggaag
     8221 ctggagcact tgatcacaga gctggttcac caaagagacg ttaccaggtc cttgatcaaa
     8281 agcaagatcg acaacgccaa atctttcgaa tggctcagcc agatgcgatt ttactttgac
     8341 cctaagcaaa cggacgtgtt acagcagctg tccattcaga tggccaatgc caagtttaac
     8401 tacggctttg agtacctggg tgttcaagac aagctggttc agacgcccct cactgaccgt
     8461 tgctatttga caatgacaca agctttggag gccaggcttg ggggctcccc atttggacct
     8521 gctggaaccg gaaagacaga gtccgtcaaa gccctcggcc atcagctggg gcggttcgtc
     8581 ttagttttca actgtgacga aacctttgat ttccaggcga tgggacgtat ctttgtgggg
     8641 ctttgccaag tgggcgcgtg gggctgcttt gatgagttta accgcctgga ggagcggatg
     8701 ctctcagctg tatcccagca ggtgcagtgc atacaagagg cactgcggga gcattccaac
     8761 cccaactacg acaagacctc agcccccatt acttgtgagc tgctgaacaa acaagtcaag
     8821 gttagcccag acatggctat cttcatcacc atgaacccag gctatgcagg tcgctcaaac
     8881 ctccccgaca acctcaagaa gctcttccgg agcttggcga tgaccaagcc tgaccggcag
     8941 ctgatcgccc aggtcatgct gtactcgcag ggcttccgga ctgctgaggt ccttgccaac
     9001 aagatcgtcc cgttcttcaa actgtgtgat gagcaactgt cttctcaaag ccattatgac
     9061 tttgggcttc gggctttgaa gagtgtgctt gtaagtgcag gcaatgtgaa gagggagagg
     9121 atccagaaga taaagaggga gaaagaagaa cggggggaag cagttgatga aggagagatt
     9181 gctgagaacc tacctgagca ggagattctg atccagagtg tctgcgagac aatggtgccc
     9241 aagctggtgg cagaagatat cccgctgctc ttcagcctcc tgtctgacgt gtttcctgga
     9301 gtccagtacc accgtgggga gatgacagcc cttcgagaag agctgaagaa agtgtgtcag
     9361 gagatgtact tgacatatgg cgacggagaa gaagttggcg ggatgtgggt tgaaaaggtt
     9421 ctccagctct atcagatcac gcagattaac cacggcctga tgatggttgg gccttcagga
     9481 agtgggaaga gcatggcctg gcgagtgctg ctgaaggctc tagagaggct tgagggtgtg
     9541 gagggcgtgg cccacataat tgaccccaag gccatcagca aagaccacct ctacggaacc
     9601 ttggatccca acaccaggga gtggacagac ggactcttca cacacgtgct gagaaagatc
     9661 atagacaatg tgagaggtga gctgcagaaa cgccagtgga ttgtctttga tggcgatgtg
     9721 gaccccgagt gggtggagaa cctgaactcc gtgctggatg acaacaagct cctgacgctg
     9781 cctaatggag agcgccttag cctgcctccc aatgtgcgaa taatgtttga agtgcaggac
     9841 ttgaaatacg cgacgctggc cacggtgtct cgctgtggca tggtgtggtt cagtgaggac
     9901 gtcctgagca cagacatgat cttcaataac ttcctggcca ggctgcgcag cattcccctg
     9961 gatgaagggg aggacgaggc acagcgtagg cgaaaaggca aagaagacga gggcgaagag
    10021 gccgcttctc caatgctgca gattcaaaga gatgcagcaa cgatcatgca gccgtacttc
    10081 acgtccaacg gcctggtcac caaggcgctg gagcacgcct tcaagctgga gcacatcatg
    10141 gacctgacgc gcctgcgctg cctgggctcg ctcttctcca tgctgcacca ggcctgccgc
    10201 aacgtggcgc agtacaatgc caaccatcct gatttcccca tgcagattga gcagctggag
    10261 cgctacattc agcgctatct ggtttacgcc atcctctggt ccttatctgg agacagccgg
    10321 ctgaagatga gagcagagct gggcgagtac atccggagga tcaccactgt gccgctgccc
    10381 actgcgccca acgtccccat cattgactat gaggtgtcca tcagtggaga gtggtcgccg
    10441 tggcaggcca aggtgcctca gattgaagtg gagacacaca aggtggcagc cccagatgtc
    10501 gtcgtgccaa ccttagatac agtccgccat gaagctctcc tgtacacttg gctggctgaa
    10561 cacaaacccc tggtcttatg tggccctcct gggtctggca agaccatgac actcttcagt
    10621 gctctccggg ccttgcctga catggaggtt gtgggcctaa acttctcaag tgccactaca
    10681 ccagagctgt tgctgaagac gtttgaccac tactgcgaat acaggcgtac acctaacggt
    10741 gttgtcttgg ctcctgttca gcttggaaag tggctggtct tgttctgtga tgaaatcaac
    10801 ttgcccgata tggataaata cggcacacag agagtcatct ccttcatcag acagatggtg
    10861 gagcatggag gcttctatcg tacctcagac caaacgtggg taaagctaga gagaatccag
    10921 tttgttgggg cttgcaatcc acccactgac cctgggagaa agcccctctc acacaggttc
    10981 ttgcgccacg tgcctgttgt atatgtggat tacccaggac cagcctccct gacgcaaatc
    11041 tatggcactt ttaaccgcgc catgctgagg ctcattccct cgctccgtac ctatgcagaa
    11101 cccctcactg ccgccatggt ggagttctat accatgtcac aggagcggtt tactcaggac
    11161 acacagccac actatatcta ctcgccccgt gagatgactc ggtgggtaag aggaatcttt
    11221 gaagcactga gacccctgga gacattgcct gttgaaggtc tcattcgtat ttgggcacat
    11281 gaagccctcc gtctcttcca agacaggctt gtggaggatg aggagcggcg ctggactgac
    11341 gaaaacattg acatggttgc cttgaagcac ttccctaaca tagacaaaga gaaagccatg
    11401 agccggccta tcctgtacag caactggctg tcaaaggatt atatcccagt tgaccaagaa
    11461 gagctaaggg attatgtcaa ggctaggctg aaggtcttct atgaagaaga acttgatgtt
    11521 cccttggtct tattcaatga agttctggac catgtgctga ggatcgacag aatattccga
    11581 caacctcaag gccatctgct tctgattggt gttagtggag ctggaaaaac caccctgtct
    11641 cgctttgtcg cctggatgaa tggcttgagt gtataccaga ttaaggtcca caggaagtac
    11701 actggggaag acttcgatga agatcttagg acagtgctga gacggtctgg ctgtaaaaat
    11761 gaaaagatag cattcataat ggatgagtcc aacgtcttag attccggatt cctggaacga
    11821 atgaataccc ttctggctaa tggcgaggtt cctgggctct ttgaagggga tgagtatgcc
    11881 acgctgatga cacagtgcaa agagggggcc cagaaggagg ggctcatgct ggactcccac
    11941 gaggagccct acaagtggtt caccagccag gtgatccgca acctgcacgt ggtcttcacc
    12001 atgaacccgt cctcagaggg gctcaaggac agggcggcca cctcaccagc ccttttcaac
    12061 aggtgtgtgc tgaactggtt tggtgactgg tccactgaag cactgtatca agtcggcaaa
    12121 gaattcacca gtaagatgga cctggagaag ccgaactata tagtgcctga ctacatgcca
    12181 gttgtgtatg ataaactgcc gcagccccca acacaccggg aagccattgt gaacagctgt
    12241 gtgtttgtcc atcagactct tcaccaggct aatgcgcgcc tggcgaagcg aggtggcaga
    12301 acgatggcca tcacacctcg gcactacctg gacttcatca accactacgc caacctcttc
    12361 catgagaagc gcagcgagtt ggaggagcag cagatgcacc tgaatgttgg gctccgaaaa
    12421 atcaaagaga ccgtggacca ggtggaagaa ctgcgccgtg acctgaggat caagagccaa
    12481 gagctggagg tcaagaacgc agcggccaat gacaagctga agaaaatggt gaaagaccag
    12541 caagaggctg agaagaagaa ggtcatgagc caagaaatcc aggagcagct acacaaacag
    12601 caggaagtga tcgcagacaa gcagatgagt gtcaaggagg acctggataa agtggagcct
    12661 gctgtcatcg aggcccagaa cgctgtgaag tccatcaaga agcagcacct ggtggaggtg
    12721 aggtccatgg ccaaccctcc tgcagctgtg aagctggctc tggagtccat ctgcctgctg
    12781 cttggtgaga gcaccacgga ctggaagcag atccgctcca tcatcatgag agagaacttc
    12841 atccccacca tcgtcaactt ctcggctgag gagatcagtg atgccatacg agagaagatg
    12901 aagaaaaact acatgtccaa ccccagttac aactatgaga tcgtcaaccg ggcttccctg
    12961 gcttgtggtc ctatggtgaa gtgggcgatt gcacagctca attatgcaga catgttaaag
    13021 cgagtggagc ccctgaggaa tgagctgcag aagctggaag atgacgccaa ggacaaccag
    13081 cagaaagcca atgaggtgga gcagatgatc agggacctgg aagccagcat tgcccgctac
    13141 aaggaggagt acgctgtcct catctctgag gcccaggcca tcaaggcaga cctggcagct
    13201 gtggaagcaa aggtaaaccg gagcactgca cttctaaaga gtttatctgc cgaaagggaa
    13261 cgttgggaaa agacgagtga aactttcaaa aaccagatgt ctaccattgc tggggactgc
    13321 ctcctgtcgg cggccttcat tgcttacgct ggctactttg accagcaaat gcgccagaac
    13381 ttgttcacca cctggtctca tcacttacag caagccaaca tccagttccg cacagacatt
    13441 gcaaggacgg agtacctctc caatgctgac gaacgcctcc gctggcaggc cagctctctg
    13501 cctgccgacg acctgtgcac agagaatgcc atcatgctaa aacgattcaa taggtacccg
    13561 ctgatcatcg acccctccgg acaggccaca gaattcatca tgaatgaata taaggaccgg
    13621 aagatcacaa ggaccagctt cttagatgat gccttccgga agaatttgga gagtgctctg
    13681 agatttggca atccccttct tgtccaggat gtggaaagct acgatccagt tttgaatcca
    13741 gtactgaacc gtgaagttcg gcgaacagga gggagggtgc tgattaccct cggtgaccaa
    13801 gacatagacc tgtcaccatc ctttgtcatc tttctgtcta cccgagaccc aactgtggag
    13861 ttcccaccag acctctgctc ccgggtgacc tttgtgaact tcaccgtcac ccgcagcagt
    13921 ttacagagcc agtgtctcaa tgaagtgctt aaagcagaaa ggccagacgt ggacgagaag
    13981 cgctccgacc tcctgaaact gcaaggggag ttccaactcc gtttacggca gcttgaaaag
    14041 tctctgctcc aagctctgaa tgaggtaaaa ggccgcattc tggatgacga caccatcatt
    14101 acaaccctgg agaatctgaa gcgggaagct gccgaggtta ccagaaaggt ggaggagaca
    14161 gatattgtca tgcaggaggt ggagaccgtg tcccagcagt acctgccgct gtccactgcc
    14221 tgcagcagca tctacttcac tatggagtcc ttgaagcagg ttcacttcct gtaccagtac
    14281 tctctgcagt tcttcctgga catctaccac aacgtgctgt atgagaaccc caacctgaag
    14341 ggtgccaccg atcacacaca gcgcctctcc atcataacca aggacctctt ccaggtggcc
    14401 tttaaccgag tggctcgagg catgctgcac caggaccaca tcaccttcgc catgctgcta
    14461 gcgaggatca agctgaaagg caccgtgggg gagcccacct atgatgcaga gttccagcac
    14521 ttcctcagag ggaaggagat tgtgctgagt gctggctcca cccccaaaat ccagggcctg
    14581 accgtagagc aggcagaagc tgtcgtgagg ttgagctgtc tgcctgcatt taaagatctg
    14641 attgccaagg tgcaggcaga tgagcagttt ggcatttggc tggatagcag ttcaccagag
    14701 cagaccgtgc catacctctg gagcgaggaa acgcctacaa ctcccattgg gcaggccatc
    14761 caccgcctgc tcctgattca agccttccga cccgaccgcc tgttggccat ggcccacatg
    14821 tttgtttcca caaacctcgg agagtccttc atgtccatca tggagcagcc tctcgacctc
    14881 acccacattg tgggcacaga ggtgaagcca aacacacctg tcctgatgtg ctctgtgcct
    14941 gggtatgatg ccagcggaca tgttgaggac ctcgcagcag agcagaacac gcagatcacg
    15001 tctattgcca tcggctcagc agaaggcttt aaccaagcag ataaggccat caacaccgcc
    15061 gtgaagtcag gcaggtgggt gatgttgaag aacgtgcacc tggccccggg ctggctgatg
    15121 cagctggaga agaagctgca ctccctgcag ccccacgcct gcttccgact tttcctcacc
    15181 atggagatca accccaaggt gcccgtgaac ctgctccgtg ctggtcggat ctttgtgttt
    15241 gagccaccac ccggagtgaa agccaacatg ctgaggacat ttagcagcat ccctgtctct
    15301 aggatatgca agtctccaaa tgagcgcgcc cgcttgtatt tcctgctggc ttggttccat
    15361 gccatcatcc aagagcgcct gcgctatgct ccactggggt ggtcaaagaa gtacgaattt
    15421 ggagaatctg accttcgatc agcttgtgac acggtggaca cgtggctgga cgacacagcc
    15481 aagggccggc agaacatctc accggataag atcccatggt ctgccctgaa gaccctgatg
    15541 gcgcagtcca tctatggtgg gcgggtggac aatgagttcg accagcgtct gctcaacacc
    15601 ttcctggagc ggctgttcac cacccgcagc ttcgacagtg aattcaagct ggcgtgcaag
    15661 gttgacgggc acaaggacat ccagatgcct gatggtatca ggcgagagga gtttgtgcag
    15721 tgggtggagc tgctgcctga cgcccagaca ccctcatggc tgggcctccc caacaatgct
    15781 gaaagggtcc tgcttaccac ccaaggcgtg gacatgatca gcaagatgct gaagatgcag
    15841 atgctggagg acgaggatga cctggcctac gcagagacgg agaagaaggc aaggacggac
    15901 tccacctccg atggccgtcc agcctggatg aggacactgc acaccacggc gtccaactgg
    15961 ctgcacctca tcccacagac actgagcccc ctgaagcgca ccgtggagaa catcaaggat
    16021 cctttgttta ggttctttga gagagaagta aagatggggg ccaagttact ccaagatgtg
    16081 cgccaagatc ttgctgacgt cgttcaagtg tgcgagggga aaaagaagca gacaaactac
    16141 ctgcgcacgc tcatcaatga gctggtgaaa gggatcttgc ctcggagctg gtcccattac
    16201 acagtgcctg caggcatgac tgtgatccag tgggtgtcag atttcagcga gaggatcaaa
    16261 cagctgcaga acatctcaca agcagctgca tctggtggtg ccaaggaact gaagaacatc
    16321 cacgtgtgcc tgggtggcct gtttgtacct gaggcctaca tcactgccac cagacagtat
    16381 gtggcacaag ctaacagctg gtccctggag gaactctgcc tggaagtcaa tgtcaccgcc
    16441 tcacagagtg ccacccttga tgcctgcagc tttggagtca caggtctgaa gctccaaggg
    16501 gccacgtgca gcaacaacaa gctatcactc tccaatgcca tctccacagt cctccccctc
    16561 acacagctgc gctgggtgaa acaaacaagc gccgagaaga aggccagcgt ggtgacctta
    16621 cctgtctacc tgaacttcac ccgggcagac ctcatcttca ccgtggactt tgaaattgct
    16681 acaaaggaag atcctcgcag cttctatgag cgtggagttg ccgttctgtg cactgagtaa
    16741 aagcttcgaa ttctgcagtc gacggtaccg cgggcccggg atccaccgga tctagataag
    16801 taatgatcat
//
