LOCUS       sequence-381784-        5809 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Includes the promoter (1kb) of SMTS with mutated STAT1/2 binding 
            site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Leisegang MS, Bibli SI, Gunther S, Pfluger-Muller B, Oo JA, Hoper C,
            Seredinski S, Yekelchyk M, Schmitz-Rixen T, Schurmann C, Hu J, Looso
            M, Sigala F, Boon RA, Fleming I, Brandes RP
  TITLE     Pleiotropic effects of laminar flow and statins depend on the 
            Kruppel-like factor-induced lncRNA MANTIS.
  JOURNAL   Eur Heart J. 2019 Aug 7;40(30):2523-2533. doi: 
            10.1093/eurheartj/ehz393.
  PUBMED    31222221
REFERENCE   2  (bases 1 to 5809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Eur Heart J"; date: "2019-08-7- 7"; volume: "40"; issue: "30"; 
            pages: "2523-2533"
COMMENT     Sequence Label: pGL3-pSMANTIS_STAT1-2_Mutant
FEATURES             Location/Qualifiers
     source          1..5809
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1073..1082
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1079..2731
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1145..1163)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    2772..2893
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2783..2802)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2837..2856
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3141..3158)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3312..3900)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3392..3411)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4071..4862,4863..4931))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4694..4713
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4932..5036)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5063..5518
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5150..5169)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5360..5381
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    5649..5697
                     /note="synthetic polyadenylation signal"
     misc_feature    5711..5802
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5751..5770
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggtaccctat gtctgacaca tagtagagct taataaacat tttctattat tgctacggtt
       61 gttgttactg ttagcaacat tgaggctgag taaaatgaat tctttttgga tagaaagatg
      121 gtctcatggt caagtgtttt tacccaaatc tcaaggagat tctaaatagc agctaagaac
      181 caggggatac ttctctggcc aaaagtctac tgagacaagg tcttgggggc agaggctagg
      241 ccaggtagtt tgtggtgcac atagaatcac tgtacctacc cagcatgaag gtgttggccc
      301 ccaaggagct aactggaggg tagcatgctg gaagacacta ccagtgttat aggaggcaaa
      361 aagaaattat ttaggtagac atttagggta aagtgagtcc ccagcagaaa actttccttt
      421 taacaaaaag cagctcaaaa atagctccct ttctaaccac acacagttaa aaaaaatcac
      481 ttctcttcta acaacaagta acctgaaagt tcaggctgta aaatacagat aagacagctc
      541 aagcacagaa gaaggggtgg aagtctcctg ggtaattgcc aaacttcaca cttatacaat
      601 gggccccagt aaaacagtgg gtcttaataa gcacattcct ttccctttag gtgcactaag
      661 atagcacgcg gcaagcagac tcatggggcg gatgcctgca gctgcaagaa gatgtatggg
      721 aacagacaca gaaactctcc ctcccagata agcaagacaa agaaacacag actaagagtc
      781 agcctatgtg gtcagggaat gggataaaag ctgataaaaa aaattctgct ctatatagat
      841 agcacacttg gtcgcagcta aaccatcagg ccctaagagg ataagacatc ccctcctcat
      901 gaggcccctc ctccctagac catttataaa aaccctgaca tttttactac tgcttagcaa
      961 cctgctcggg acccctctct gtgactgtga gctgttctgt tcttttgcct agctagcccg
     1021 ggctcgagat ctgcgatcta agtaagcttg gcattccggt actgttggta aagccaccat
     1081 ggaagacgcc aaaaacataa agaaaggccc ggcgccattc tatccgctgg aagatggaac
     1141 cgctggagag caactgcata aggctatgaa gagatacgcc ctggttcctg gaacaattgc
     1201 ttttacagat gcacatatcg aggtggacat cacttacgct gagtacttcg aaatgtccgt
     1261 tcggttggca gaagctatga aacgatatgg gctgaataca aatcacagaa tcgtcgtatg
     1321 cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta tcggagttgc
     1381 agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta tgggcatttc
     1441 gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa
     1501 aaagctccca atcatccaaa aaattattat catggattct aaaacggatt accagggatt
     1561 tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg aatacgattt
     1621 tgtgccagag tccttcgata gggacaagac aattgcactg atcatgaact cctctggatc
     1681 tactggtctg cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga gattctcgca
     1741 tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt taagtgttgt
     1801 tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat gtggatttcg
     1861 agtcgtctta atgtatagat ttgaagaaga gctgtttctg aggagccttc aggattacaa
     1921 gattcaaagt gcgctgctgg tgccaaccct attctccttc ttcgccaaaa gcactctgat
     1981 tgacaaatac gatttatcta atttacacga aattgcttct ggtggcgctc ccctctctaa
     2041 ggaagtcggg gaagcggttg ccaagaggtt ccatctgcca ggtatcaggc aaggatatgg
     2101 gctcactgag actacatcag ctattctgat tacacccgag ggggatgata aaccgggcgc
     2161 ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg gatctggata ccgggaaaac
     2221 gctgggcgtt aatcaaagag gcgaactgtg tgtgagaggt cctatgatta tgtccggtta
     2281 tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggc tacattctgg
     2341 agacatagct tactgggacg aagacgaaca cttcttcatc gttgaccgcc tgaagtctct
     2401 gattaagtac aaaggctatc aggtggctcc cgctgaattg gaatccatct tgctccaaca
     2461 ccccaacatc ttcgacgcag gtgtcgcagg tcttcccgac gatgacgccg gtgaacttcc
     2521 cgccgccgtt gttgttttgg agcacggaaa gacgatgacg gaaaaagaga tcgtggatta
     2581 cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga
     2641 agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga tcctcataaa
     2701 ggccaagaag ggcggaaaga tcgccgtgta attctagagt cggggcggcc ggccgcttcg
     2761 agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     2821 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     2881 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     2941 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc
     3001 gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca
     3061 tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc
     3121 cggcagcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     3181 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     3241 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     3301 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     3361 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     3421 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     3481 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     3541 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     3601 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     3661 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     3721 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     3781 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     3841 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     3901 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     3961 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     4021 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     4081 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     4141 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     4201 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     4261 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     4321 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     4381 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     4441 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     4501 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     4561 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     4621 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     4681 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     4741 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     4801 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     4861 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     4921 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     4981 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     5041 tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     5101 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     5161 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     5221 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     5281 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     5341 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     5401 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     5461 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttgc
     5521 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     5581 ttacgccagc ccaagctacc atgataagta agtaatatta aggtacggga ggtacttgga
     5641 gcggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat
     5701 cgatagtact aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat
     5761 aggctgtccc cagtgcaagt gcaggtgcca gaacatttct ctatcgata
//
