LOCUS       sequence-381819-        3757 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synAR Part (Part 3).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Sanford A, Kiriakov S, Khalil AS
  TITLE     A Toolkit for Precise, Multigene Control in Saccharomyces 
            cerevisiae.
  JOURNAL   ACS Synth Biol. 2022 Nov 11. doi: 10.1021/acssynbio.2c00423.
  PUBMED    36367334
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2022 Nov 11. doi: 10.1021/acssynbio.2c00423."
COMMENT     Sequence Label: pAS154
FEATURES             Location/Qualifiers
     source          1..3757
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(2127..2161)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(2232..2891)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2806..2825
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      3073..3661
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3562..3581
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgtagtcggt ctcatatgct cgagaaaaaa atggagcgcc ccttccagtg tcgcatttgc
       61 atgcggaact tttcggattc tccaactttg agaagacata cccgtactca taccggtgaa
      121 aaaccgtttc agtgtcggat ctgtatgcga aatttctccc aaagatcatc tttggttaga
      181 catctacgta cgcacaccgg cgagaagcca ttccaatgcc gaatatgcat gcgcaacttc
      241 agtgaaagag gtaatttgac tagacaccta aaaacccacc tgagaggaga tggtgctgga
      301 ttgattgata tcgatctgaa gaaacttggt aatctgaaac tacaggagga aggagaggct
      361 tccagcacca ccagccccac tgaggagaca acccagaagc tgacagtgtc acacattgaa
      421 ggctatgaat gtcagcccat ctttctgaat gtcctggaag ccattgagcc aggtgtagtg
      481 tgtgctggac acgacaacaa ccagcccgac tcctttgcag ccttgctctc tagcctcaat
      541 gaactgggag agagacagct tgtacacgtg gtcaagtggg ccaaggcctt gcctggcttc
      601 cgcaacttac acgtggacga ccagatggct gtcattcagt actcctggat ggggctcatg
      661 gtgtttgcca tgggctggcg atccttcacc aatgtcaact ccaggatgct ctacttcgcc
      721 cctgatctgg ttttcaatga gtaccgcatg cacaagtccc ggatgtacag ccagtgtgtc
      781 cgaatgaggc acctccctca agagtttgga tggctccaaa tcacccccca ggaattcctg
      841 tgcatgaaag cactgctact cttcagcatt attccagtgg atgggctgaa aaatcaaaaa
      901 ttctttgatg aacttcgaat gaactacatc aaggaactcg atcgtatcat tgcatgcaaa
      961 agaaaaaatc ccacatcctg ctcaagacgc ttctaccagc tcaccaagct cctggactcc
     1021 gtgcagccta ttgcgagaga gctgcatcag ttcacttttg acctgctaat caagtcacac
     1081 atggtgagcg tggactttcc ggaaatgatg gcagagatca tctctgtgca agtgcccaag
     1141 atcctttctg ggaaagtcaa gcccatctat ttccacaccc agggcgacgg agcaggctta
     1201 ataaatgcta gcatgacggt cgaccatgat ttcaatagcg aagatatttt attccccata
     1261 gaaagcatga gtagtataca atacgtggag aataataacc caaataatat taacaacgat
     1321 gttatcccgt attctctaga tatcaaaaac actgtcttag atagtgcgga tctcaatgac
     1381 attcaaaatc aagaaacttc actgaatttg gggcttcctc cactatcttt cgactctcca
     1441 ctgcccgtaa cggaaacgat accatccact accgataaca gcttgcattt gaaagctgat
     1501 agcaacaaaa atcgcgatgc aagaactatt gaaaatgata gtgaaattaa gagtactaat
     1561 aatgctagtg gctctggggc aaatcaatac acaactctta cttcacctta tcctatgaac
     1621 gacattttgt acaacatgaa caatccgtta caatcaccgt caccttcatc ggtacctcaa
     1681 aatccgacta taaatcctcc cataaataca gcaagtaacg aaactaattt atcgcctcaa
     1741 acttcaaatg gtaatgaaac tcttatatct ccacgagccc aacaacatac gtccattaaa
     1801 gataatcgtc tgtccttacc taatggtgct aattcgaatc ttttcattga cactaaccca
     1861 aacaatttga acgaaaaact aagaaatcaa ttgaactcag atacaaattc atattctaac
     1921 tccatttcta attcaaactc caattctacg ggtaatttaa attccagtta ttttaattca
     1981 ctgaacatag actccatgct agatgattac gtttctagtg atctcttatt gaatgatgat
     2041 gatgatgaca ctaatttatc acgccgaaga tttagcgacg ttataacaaa ccaagagctc
     2101 taagggccca tcctgagacc agaccaataa aaaacgcccg gcggcaaccg agcgttctga
     2161 acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc
     2221 tcgatatcaa attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc
     2281 attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc
     2341 agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg
     2401 tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgaaacg
     2461 aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc
     2521 acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc
     2581 gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat
     2641 atcaccagct caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg
     2701 gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa
     2761 aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat
     2821 gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt
     2881 tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc
     2941 ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccc gatcaatcat
     3001 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     3061 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3121 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3181 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     3241 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     3301 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     3361 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     3421 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     3481 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     3541 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     3601 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     3661 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     3721 gttctttcct gcgttatccc ctgattctgt ggataac
//
