LOCUS       sequence-381822-        3966 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synDHBR Part (Part 3).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Sanford A, Kiriakov S, Khalil AS
  TITLE     A Toolkit for Precise, Multigene Control in Saccharomyces 
            cerevisiae.
  JOURNAL   ACS Synth Biol. 2022 Nov 11. doi: 10.1021/acssynbio.2c00423.
  PUBMED    36367334
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2022 Nov 11. doi: 10.1021/acssynbio.2c00423."
COMMENT     Sequence Label: pAS155
FEATURES             Location/Qualifiers
     source          1..3966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1745..2314
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     terminator      complement(2341..2375)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(2446..3105)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3020..3039
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      3287..3875
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3776..3795
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ataaccgtag tcggtctcat atgctcgaga aaaaaatgga gcgacccttc cagtgcagga
       61 tttgcatgcg gaacttttcg agacaatcta atttgtcaag acatacaagg actcataccg
      121 gtgaaaaacc gtttcagtgt aggatctgta tgcgaaattt cagtagaaat gaacatttgg
      181 ttctacatct acgtacgcac acaggcgaaa agccattcca atgccgaata tgcatgcgca
      241 acttcagtca aaaaactggt cttagagttc atctaaaaac ccaccttaga ggagatggtg
      301 ctggattaat tgatatcgat atgggtacaa gaccatatgc ttgtccagtt gagtcttgtg
      361 acagaagatt ctcaagaagc gacgaattaa cgcgtcacat tagaattcat acaggtcaaa
      421 aaccttttca gtgtagaatc tgcatgagaa acttttcacg ttcggatcac cttactactc
      481 atatcagaac gcacactggt gaaaaacctt ttgcatgtga tatttgtgga agaaaattcg
      541 caagatctga tgaaagaaag cgtcacacaa aaatccacac tggtgggggc ggaactccag
      601 ctgctgcttc caccttggaa gatcctagtg ctggggatat gcgtgctgct aacttgtggc
      661 cttctccatt aatgataaaa agatctaaaa agaattctct tgctttgtcg ttgactgcag
      721 atcaaatggt ttctgcttta ttagacgccg aacctcctat tttatactct gaatatgatc
      781 ctactaggcc attttcggaa gcctctatga tgggtttaat taccaatttg atggatagag
      841 aattagtgca tatgattaat tgggccaaga gggtccctgg ttttgttgat ttaaccttac
      901 acgatcaagt acatttatta gaatgtgctt ggcttgaaat cttgcaaatt ggtctagttt
      961 ggagatccat ggaacatcca gttaaattac tattcgctcc taatttgctt tggacagaaa
     1021 ccaaggtaag tgtgtagaag gaatggttga aatttttgat atgctacttg ctacaagctc
     1081 aagattcaga atgatgaact tgcaaggtga ggaattcgtt tgtttgaaat cgattatact
     1141 actcaattct ggagtttaca ctttcttgtc atccacgtta aagtctttag aagaaaagga
     1201 ccacatacat agggtccttg acaagatcac tgacacatta attcatttaa tggccaaggc
     1261 tggtttaacc ttgcaacaac aacaccaacg tttggcccaa ttgttgttga tattgagtca
     1321 catacgtcat atgtctaata aatctatgga acacctagac tccatgaagt gtaagaacgt
     1381 cgtaccccta tacgacttat tgttagaaat gttggatgca catagattac atgcccctac
     1441 tagtagaggt ggagcatcag tagaggaaac tgatcaatcg catttggcta ctgcaggttc
     1501 cactagttcc gaattacatt tagatggtga ggatgtagct atggcccacg cagatgcgct
     1561 agatgatttt gatctagaca tgctcggtga cggggactcc cctggtccgg ggtttacccc
     1621 acatgattcc gccccatatg gcgctttgga tatggctgac tttgaattcg aacaaatgtt
     1681 tactgatgca ttgggtatcg atgaatatgg tggtggcgac ggagcaggct taataaatgc
     1741 tagcagggat tccagggaag ggatgttttt gccgaaacct gaagcgggct cagcgattag
     1801 tgacgtgttt gagggccgcg aggtgtgcca gccaaaacga ataaggccat ttcatcctcc
     1861 aggaagtcca tgggccaatc gcccactccc cgcaagcctc gcaccaacac caaccggtcc
     1921 agtacatgag ccagtcgggt cactgacacc ggcaccagtc cctcagccac tggatccagc
     1981 gcccgcagtg actcccgagg cgagtcacct attggaggat cccgatgaag aaacaagcca
     2041 ggctgtcaaa gcccttcggg agatggccga tacggtgatt cctcagaaag aagaagcggc
     2101 aatttgtggc caaatggacc tttcacatcc gccccctagg ggccatctgg atgaactgac
     2161 aaccacactt gagtcaatga ccgaggatct gaacctggac tcacccctga cgccggaact
     2221 taatgagatt ctggatacat tcctgaacga cgagtgcctc ttacatgcaa tgcatataag
     2281 cacaggacta tcaatctttg acacatctct gttttaaggg cccatcctga gaccagacca
     2341 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc
     2401 attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg
     2461 ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
     2521 aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
     2581 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
     2641 actggtgaaa ctcacccagg gattggctga aacgaaaaac atattctcaa taaacccttt
     2701 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa
     2761 ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg
     2821 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc
     2881 catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata
     2941 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt
     3001 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca
     3061 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
     3121 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg
     3181 aaagttggaa cctcttacgt gcccgatcaa tcatgaccaa aatcccttaa cgtgagtttt
     3241 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3301 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3361 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3421 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3481 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3541 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3601 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3661 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3721 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3781 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3841 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3901 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     3961 ctgtgg
//
