LOCUS       sequence-381824-        3142 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synER Part (Part 3).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Sanford A, Kiriakov S, Khalil AS
  TITLE     A Toolkit for Precise, Multigene Control in Saccharomyces 
            cerevisiae.
  JOURNAL   ACS Synth Biol. 2022 Nov 11. doi: 10.1021/acssynbio.2c00423.
  PUBMED    36367334
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2022 Nov 11. doi: 10.1021/acssynbio.2c00423."
COMMENT     Sequence Label: pAS156
FEATURES             Location/Qualifiers
     source          1..3142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1511..1545)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1616..2275)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2190..2209
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2457..3045
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2946..2965
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtagtcggtc tcatatgctc gagaaaaaaa tggagcgtcc cttccaatgt agaatttgca
       61 tgagaaactt ttcgagacaa tctaatttgt caagacatac ccgtactcat accggtgaaa
      121 aaccgtttca gtgtcggata tgtatgcgaa atttctcaag aaatgaacat ttggttttgc
      181 atctacgtac gcacaccggc gagaagccat tccaatgcag aatatgtatg agaaacttta
      241 gtcaaaaaac tggtttgaga gttcacctaa aaacacatct gagaggtgat ggtgctggat
      301 tgattgatat cgattctgct ggtgacatga gagctgcaaa cctttggcca agcccgttaa
      361 tgattaaacg atctaagaaa aacagtttag ctttgtccct gacggctgat caaatggtaa
      421 gtgccttgtt ggatgctgaa cctccaatac tctattcaga atatgatcct acaagacctt
      481 tcagtgaagc ttcgatgatg ggcttactta ctaacttagc agacagggag ttagttcaca
      541 tgatcaactg ggcaaaaagg gtaccaggtt ttgttgattt gaccttacat gatcaagtcc
      601 accttctaga atgtgcctgg ctagaaatcc taatgattgg acttgtctgg cgctcaatgg
      661 aacacccagt gaagctactt tttgctccta atttgttatt ggacaggaat caaggaaaat
      721 gtgtagaggg catggttgag attttcgaca tgttgttagc tacatcatct agattccgca
      781 tgatgaatct gcaaggtgaa gagtttgtgt gcctcaaatc tattattttg cttaattctg
      841 gtgtgtacac atttttgtcc agcactctga agtctctgga agaaaaagat catattcata
      901 gagtcctgga caagatcaca gatactttga tccacttaat ggcaaaagca ggcttaaccc
      961 ttcagcagca gcaccagagg ttagctcaat tattactcat cttatcccac attaggcata
     1021 tgagtaacaa agggatggaa catctttata gtatgaagtg caagaatgtc gtgccactct
     1081 atgatctgtt gttagaaatg ttagacgccc ataggctaca tgcgccaact tctcgtggag
     1141 gggcatccgt tgaagaaaca gaccaaagcc atttggccac tgcaggttct acttcatcgc
     1201 attccttgca aaagtattac ataacgggtg aagcagaagg tttccctgcc acagttggtg
     1261 atggagcagg cttaataaat gctagccatt tagatggcga ggacgtagcg atggcgcatg
     1321 ctgatgcgct agatgatttt gatttagaca tgttggggga tggtgattca cctggtccag
     1381 gatttactcc ccacgactcc gccccctacg gtgctctaga tatggccgac ttcgaatttg
     1441 aacaaatgtt taccgatgca cttggaattg acgagtacgg tgggtaaggg cccatcctga
     1501 gaccagacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
     1561 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg
     1621 ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga
     1681 agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
     1741 gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt
     1801 ttaaatcaaa actggtgaaa ctcacccagg gattggctga aacgaaaaac atattctcaa
     1861 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata
     1921 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag
     1981 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
     2041 ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa
     2101 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
     2161 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
     2221 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
     2281 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
     2341 cattatggtg aaagttggaa cctcttacgt gcccgatcaa tcatgaccaa aatcccttaa
     2401 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     2461 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     2521 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     2581 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     2641 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     2701 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     2761 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     2821 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     2881 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     2941 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     3001 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     3061 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     3121 tcccctgatt ctgtggataa cc
//
