LOCUS       sequence-381827-        3847 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synMR Part (Part 3).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Sanford A, Kiriakov S, Khalil AS
  TITLE     A Toolkit for Precise, Multigene Control in Saccharomyces 
            cerevisiae.
  JOURNAL   ACS Synth Biol. 2022 Nov 11. doi: 10.1021/acssynbio.2c00423.
  PUBMED    36367334
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2022 Nov 11. doi: 10.1021/acssynbio.2c00423."
COMMENT     Sequence Label: pAS158
FEATURES             Location/Qualifiers
     source          1..3847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(2217..2251)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(2322..2981)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2896..2915
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      3163..3751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3652..3671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgtagtcggt ctcatatgct cgagaaaaaa atgggtaccc gcccatatgc ttgccctgtc
       61 gagtcctgcg atcgccgctt ttctcgctcg gatgagctta cccgccatat ccgcatccat
      121 accggtcaga agcccttcca gtgtcgaatc tgcatgcgta acttcagtcg tagtgaccac
      181 cttaccaccc acatccgcac ccacacaggc gagaagcctt ttgcctgtga catttgtggg
      241 aggaagtttg ccaggagtga tgaacgcaag aggcatacca aaatccatac aggtggagat
      301 ggtgctggat tgattgatat cgataattta ggagcacgaa agtcaaagaa gttgggaaag
      361 ttaaaaggga ttcacgagga gcagccacag cagcagcagc ccccaccccc acccccaccc
      421 ccgcaaagcc cagaggaagg gacaacgtac atcgctcctg caaaagaacc ctcggtcaac
      481 acagcactgg ttcctcagct ctccacaatc tcacgagcgc tcacaccttc ccccgttatg
      541 gtccttgaaa acattgaacc tgaaattgta tatgcaggct atgacagctc aaaaccagat
      601 acagccgaaa atctgctctc cacgctcaac cgcttagcag gcaaacagat gatccaagtc
      661 gtgaagtggg caaaggtact tccaggattt aaaaacttgc ctcttgagga ccaaattacc
      721 ctaatccggt attcttggat gtgtctatca tcatttgcct tgagctggag atcgtacaaa
      781 catacgaaca gccaatttct ctattttgca ccagacctag tctttaatga agagaagatg
      841 catcagtctg ccatgtatga actatgccag gggatgcacc aaatcagcct tcagttcgtt
      901 cgactgcagc tcacctttga agaatacacc atcatgaaag ttttgctgct actaagcaca
      961 attccaaagg atggcctcaa aagccaggct gcatttgaag aaatgaggac aaattacatc
     1021 aaagaactga ggaagatggt aactaagtgt cccaacaatt ctgggcagag ctggcagagg
     1081 ttctaccaac tgaccaagct gctggactcc atgcatgacc tggtgagcga cctgctggaa
     1141 ttctgcttct acaccttccg agagtcccat gcgctgaagg tagagttccc cgcaatgctg
     1201 gtggagatca tcagcgacca gctgcccaag gtggagtcgg ggaacgccaa gccgctctac
     1261 ttccaccgga agggcgacgg agcaggctta ataaatgcta gcatgacggt cgaccatgat
     1321 ttcaatagcg aagatatttt attccccata gaaagcatga gtagtataca atacgtggag
     1381 aataataacc caaataatat taacaacgat gttatcccgt attctctaga tatcaaaaac
     1441 actgtcttag atagtgcgga tctcaatgac attcaaaatc aagaaacttc actgaatttg
     1501 gggcttcctc cactatcttt cgactctcca ctgcccgtaa cggaaacgat accatccact
     1561 accgataaca gcttgcattt gaaagctgat agcaacaaaa atcgcgatgc aagaactatt
     1621 gaaaatgata gtgaaattaa gagtactaat aatgctagtg gctctggggc aaatcaatac
     1681 acaactctta cttcacctta tcctatgaac gacattttgt acaacatgaa caatccgtta
     1741 caatcaccgt caccttcatc ggtacctcaa aatccgacta taaatcctcc cataaataca
     1801 gcaagtaacg aaactaattt atcgcctcaa acttcaaatg gtaatgaaac tcttatatct
     1861 ccacgagccc aacaacatac gtccattaaa gataatcgtc tgtccttacc taatggtgct
     1921 aattcgaatc ttttcattga cactaaccca aacaatttga acgaaaaact aagaaatcaa
     1981 ttgaactcag atacaaattc atattctaac tccatttcta attcaaactc caattctacg
     2041 ggtaatttaa attccagtta ttttaattca ctgaacatag actccatgct agatgattac
     2101 gtttctagtg atctcttatt gaatgatgat gatgatgaca ctaatttatc acgccgaaga
     2161 tttagcgacg ttataacaaa ccaagagctc taagggccca tcctgagacc agaccaataa
     2221 aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta
     2281 ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac
     2341 tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg
     2401 gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg
     2461 cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg
     2521 gtgaaactca cccagggatt ggctgaaacg aaaaacatat tctcaataaa ccctttaggg
     2581 aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc
     2641 cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa
     2701 acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata
     2761 cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac
     2821 ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg
     2881 ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg
     2941 gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct
     3001 gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag
     3061 ttggaacctc ttacgtgccc gatcaatcat gaccaaaatc ccttaacgtg agttttcgtt
     3121 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     3181 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     3241 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     3301 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     3361 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     3421 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     3481 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     3541 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     3601 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     3661 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     3721 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     3781 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     3841 ggataac
//
