LOCUS       sequence-381828-        5279 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synAR Cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5279)
  AUTHORS   Sanford A, Kiriakov S, Khalil AS
  TITLE     A Toolkit for Precise, Multigene Control in Saccharomyces 
            cerevisiae.
  JOURNAL   ACS Synth Biol. 2022 Nov 11. doi: 10.1021/acssynbio.2c00423.
  PUBMED    36367334
REFERENCE   2  (bases 1 to 5279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2022 Nov 11. doi: 10.1021/acssynbio.2c00423."
COMMENT     Sequence Label: pAS159
FEATURES             Location/Qualifiers
     source          1..5279
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        471..884
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     terminator      3011..3198
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3066..3090)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(join(3515..4306,4307..4375))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4138..4157
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4376..4480)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4583..5171
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5072..5091
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgaattcg catctagaac ggggtcatca cggctcatca tgcgccaaac aaatgtgtgc
       61 aatacacgct cggatgactg catgatgacc gcactgactg gggacagcag atccacctaa
      121 gcctgtgaga gaagcagaca cccgacagat caaggcagtt acgtctcacc aaaccagatg
      181 tcaacacagc tacaacgcct tgccaacagg gagttcttca gagacatgga ggctcaaaac
      241 gaaattattg acagcctaga catcaatagt catacaacag aaagcgacca cccaactttg
      301 gctgataata gcgtataaac aatgcatact ttgtacgttc aaaatacaat gcagtagata
      361 tatttatgca tattacatat aatacatatc acataggaag caacaggcgc gttggacttt
      421 taattttcga ggaccgcgaa tccttacatc acacccaatc ccccacaagt gatcccccac
      481 acaccatagc ttcaaaatgt ttctactcct tttttactct tccagatttt ctcggactcc
      541 gcgcatcgcc gtaccacttc aaaacaccca agcacagcat actaaatttc ccctctttct
      601 tcctctaggg tgtcgttaat tacccgtact aaaggtttgg aaaagaaaaa agacaccgcc
      661 tcgtttcttt ttcttcgtcg aaaaaggcaa taaaaatttt tatcacgttt ctttttcttg
      721 aaaatttttt tttttgattt ttttctcttt cgatgacctc ccattgatat ttaagttaat
      781 aaacggtcat caatttctca agtttcagtt tcatttttct tgttctatta caactttttt
      841 tacttcttgc tcattagaaa gaaagcatag caatctaatc taagttttaa ttacaaaaga
      901 tctatgctcg agaaaaaaat ggagcgcccc ttccagtgtc gcatttgcat gcggaacttt
      961 tcggattctc caactttgag aagacatacc cgtactcata ccggtgaaaa accgtttcag
     1021 tgtcggatct gtatgcgaaa tttctcccaa agatcatctt tggttagaca tctacgtacg
     1081 cacaccggcg agaagccatt ccaatgccga atatgcatgc gcaacttcag tgaaagaggt
     1141 aatttgacta gacacctaaa aacccacctg agaggagatg gtgctggatt gattgatatc
     1201 gatctgaaga aacttggtaa tctgaaacta caggaggaag gagaggcttc cagcaccacc
     1261 agccccactg aggagacaac ccagaagctg acagtgtcac acattgaagg ctatgaatgt
     1321 cagcccatct ttctgaatgt cctggaagcc attgagccag gtgtagtgtg tgctggacac
     1381 gacaacaacc agcccgactc ctttgcagcc ttgctctcta gcctcaatga actgggagag
     1441 agacagcttg tacacgtggt caagtgggcc aaggccttgc ctggcttccg caacttacac
     1501 gtggacgacc agatggctgt cattcagtac tcctggatgg ggctcatggt gtttgccatg
     1561 ggctggcgat ccttcaccaa tgtcaactcc aggatgctct acttcgcccc tgatctggtt
     1621 ttcaatgagt accgcatgca caagtcccgg atgtacagcc agtgtgtccg aatgaggcac
     1681 ctccctcaag agtttggatg gctccaaatc accccccagg aattcctgtg catgaaagca
     1741 ctgctactct tcagcattat tccagtggat gggctgaaaa atcaaaaatt ctttgatgaa
     1801 cttcgaatga actacatcaa ggaactcgat cgtatcattg catgcaaaag aaaaaatccc
     1861 acatcctgct caagacgctt ctaccagctc accaagctcc tggactccgt gcagcctatt
     1921 gcgagagagc tgcatcagtt cacttttgac ctgctaatca agtcacacat ggtgagcgtg
     1981 gactttccgg aaatgatggc agagatcatc tctgtgcaag tgcccaagat cctttctggg
     2041 aaagtcaagc ccatctattt ccacacccag ggcgacggag caggcttaat aaatgctagc
     2101 atgacggtcg accatgattt caatagcgaa gatattttat tccccataga aagcatgagt
     2161 agtatacaat acgtggagaa taataaccca aataatatta acaacgatgt tatcccgtat
     2221 tctctagata tcaaaaacac tgtcttagat agtgcggatc tcaatgacat tcaaaatcaa
     2281 gaaacttcac tgaatttggg gcttcctcca ctatctttcg actctccact gcccgtaacg
     2341 gaaacgatac catccactac cgataacagc ttgcatttga aagctgatag caacaaaaat
     2401 cgcgatgcaa gaactattga aaatgatagt gaaattaaga gtactaataa tgctagtggc
     2461 tctggggcaa atcaatacac aactcttact tcaccttatc ctatgaacga cattttgtac
     2521 aacatgaaca atccgttaca atcaccgtca ccttcatcgg tacctcaaaa tccgactata
     2581 aatcctccca taaatacagc aagtaacgaa actaatttat cgcctcaaac ttcaaatggt
     2641 aatgaaactc ttatatctcc acgagcccaa caacatacgt ccattaaaga taatcgtctg
     2701 tccttaccta atggtgctaa ttcgaatctt ttcattgaca ctaacccaaa caatttgaac
     2761 gaaaaactaa gaaatcaatt gaactcagat acaaattcat attctaactc catttctaat
     2821 tcaaactcca attctacggg taatttaaat tccagttatt ttaattcact gaacatagac
     2881 tccatgctag atgattacgt ttctagtgat ctcttattga atgatgatga tgatgacact
     2941 aatttatcac gccgaagatt tagcgacgtt ataacaaacc aagagctcta agggcccatc
     3001 ctaactcgag gcgaatttct tatgatttat gatttttatt attaaataag ttataaaaaa
     3061 aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat tcttattctt
     3121 gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagcatgagg tcgctcttat
     3181 tgaccacacc tctaccggca tgccgagcaa atgcctgcaa atcgctcccc atttcgctga
     3241 gcatgagacg gaaatctgct cgtcagtggt gctcacactg acgaatcatg tacagatcat
     3301 accgatgact gcctggcgac tcacaactaa gcaagacagc cggaaccagc gccggcgaac
     3361 accactgcat atatggcata tcacaacagt ccaactagtg cactgcagta cagcggccgc
     3421 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     3481 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     3541 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     3601 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgggacc
     3661 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     3721 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     3781 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     3841 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     3901 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     3961 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     4021 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     4081 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     4141 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     4201 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     4261 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     4321 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     4381 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     4441 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     4501 cacctgtcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     4561 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     4621 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     4681 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     4741 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     4801 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     4861 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     4921 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     4981 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     5041 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     5101 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     5161 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     5221 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt gcggccgcc
//
