LOCUS       sequence-381830-       20154 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To insert T2A-Cas9 into Gal4 coding sequence in Drosophila, 
            converting Gal4 into a T2A-Cas9 transgene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20154)
  AUTHORS   Koreman GT, Xu Y, Hu Q, Zhang Z, Allen SE, Wolfner MF, Wang B, Han C
  TITLE     Upgraded CRISPR/Cas9 tools for tissue-specific mutagenesis in 
            Drosophila.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Apr 6;118(14). pii: 2014255118. doi: 
            10.1073/pnas.2014255118.
  PUBMED    33782117
REFERENCE   2  (bases 1 to 20154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-04-6"; volume: "118(14). pii: 
            2014255118. doi"; pages: " 10.1073/pnas.2014255118"
COMMENT     Sequence Label: pHACK(Gal4)-DONR(T2A-Cas9)
FEATURES             Location/Qualifiers
     source          1..20154
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(1..31,32..233)
                     /label=P element 3' end
                     /note="P element 3' end"
     CDS             246..686
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             1368..1421
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1440..5543
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1440..5543
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1440..5543
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5559..5579
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5580..5606
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        8410..8830
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     CDS             10945..10965
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(12402..12420)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(12872..12943,13345..13618,13693..14347,14409..14724,
                     14928..15059,15130..15744)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(16523..17077,17078..17108)
                     /label=P element 5' end
     rep_origin      complement(17343..17931)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(17423..17442)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(18102..18893,18894..18962))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     18725..18744
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(18963..19067)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     19135..19153
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(19191..19213)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     19313..19332
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    19542..19611
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
       61 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
      121 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
      181 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
      241 gctgcatgaa gctactgtct tctatcgaac aagcatgcga tatttgccga cttaaaaagc
      301 tcaagtgctc caaagaaaaa ccgaagtgcg ccaagtgtct gaagaacaac tgggagtgtc
      361 gctactctcc caaaaccaaa aggtctccgc tgactagggc acatctgaca gaagtggaat
      421 caaggctaga aagactggaa cagctatttc tactgatttt tcctcgagaa gaccttgaca
      481 tgattttgaa aatggattct ttacaggata taaaagcatt gttaacagga ttatttgtac
      541 aagataatgt gaataaagat gccgtcacag atagattggc ttcagtggag actgatatgc
      601 ctctaacatt gagacagcat agaataagtg cgacatcatc atcggaagag agtagtaaca
      661 aaggtcaaag acagttgact gtatcgattg actcggcagc tcatcatgat aactccacaa
      721 ttccgttgga ttttatgccc agggatgctc ttcatggatt tgattggtct gaagaggatg
      781 acatgtcgga tggcttgccc ttcctgaaaa cggaccccaa caataatggg ttctttggcg
      841 acggttctct cttatgtatt cttcgatcta ttggctttaa accggaaaat tacacgaact
      901 ctaacgttaa caggctcccg accatgatta cggatagata cacgttggct tctagatcca
      961 caacatcccg tttacttcaa agttatctca ataattttca cccctactgc cctatcgtgc
     1021 actcaccgac gctaatgatg ttgtataata accagattga aatcgcgtcg aaggatcaat
     1081 ggcaaatcct ttttaactgc atattagcca ttggagcctg gtgtatagag ggggaatcta
     1141 ctgatataga tgttttttac tatcaaaatg ctaaatctca tttgacgagc aaggtcttcg
     1201 agtcaggttc cataattttg gtgacagccc tacatcttct gtcgcgatat acacagtgga
     1261 ggcagaaaac aaatactagc tataattttc acagcttttc cataagaatg gccatatcat
     1321 tgggcttgaa tagggacctc ccctcgtcct tcagtgatag cagcggagag ggcagaggaa
     1381 gtctgctaac atgcggtgac gtcgaggaga atcctggccc aggaagcgga ggcgctagca
     1441 tggacaagaa gtactccatt gggctcgata tcggcacaaa cagcgtcggc tgggccgtca
     1501 ttacggacga gtacaaggtg ccgagcaaaa aattcaaagt tctgggcaat accgatcgcc
     1561 acagcataaa gaagaacctc attggcgccc tcctgttcga ctccggggag acggccgaag
     1621 ccacgcggct caaaagaaca gcacggcgca gatatacccg cagaaagaat cggatctgct
     1681 acctgcagga gatctttagt aatgagatgg ctaaggtgga tgactctttc ttccataggc
     1741 tggaggagtc ctttttggtg gaggaggata aaaagcacga gcgccaccca atctttggca
     1801 atatcgtgga cgaggtggcg taccatgaaa agtacccaac catatatcat ctgaggaaga
     1861 agcttgtaga cagtactgat aaggctgact tgcggttgat ctatctcgcg ctggcgcata
     1921 tgatcaaatt tcggggacac ttcctcatcg agggggacct gaacccagac aacagcgatg
     1981 tcgacaaact ctttatccaa ctggttcaga cttacaatca gcttttcgaa gagaacccga
     2041 tcaacgcatc cggagttgac gccaaagcaa tcctgagcgc taggctgtcc aaatcccggc
     2101 ggctcgaaaa cctcatcgca cagctccctg gggagaagaa gaacggcctg tttggtaatc
     2161 ttatcgccct gtcactcggg ctgaccccca actttaaatc taacttcgac ctggccgaag
     2221 atgccaagct tcaactgagc aaagacacct acgatgatga tctcgacaat ctgctggccc
     2281 agatcggcga ccagtacgca gacctttttt tggcggcaaa gaacctgtca gacgccattc
     2341 tgctgagtga tattctgcga gtgaacacgg agatcaccaa agctccgctg agcgctagta
     2401 tgatcaagcg ctatgatgag caccaccaag acttgacttt gctgaaggcc cttgtcagac
     2461 agcaactgcc tgagaagtac aaggaaattt tcttcgatca gtctaaaaat ggctacgccg
     2521 gatacattga cggcggagca agccaggagg aattttacaa atttattaag cccatcttgg
     2581 aaaaaatgga cggcaccgag gagctgctgg taaagcttaa cagagaagat ctgttgcgca
     2641 aacagcgcac tttcgacaat ggaagcatcc cccaccagat tcacctgggc gaactgcacg
     2701 ctatactcag gcggcaagag gatttctacc cctttttgaa agataacagg gaaaagattg
     2761 agaaaatcct cacatttcgg ataccctact atgtaggccc cctcgcccgg ggaaattcca
     2821 gattcgcgtg gatgactcgc aaatcagaag agaccatcac tccctggaac ttcgaggaag
     2881 tcgtggataa gggggcctct gcccagtcct tcatcgaaag gatgactaac tttgataaaa
     2941 atctgcctaa cgaaaaggtg cttcctaaac actctctgct gtacgagtac ttcacagttt
     3001 ataacgagct caccaaggtc aaatacgtca cagaagggat gagaaagcca gcattcctgt
     3061 ctggagagca gaagaaagct atcgtggacc tcctcttcaa gacgaaccgg aaagttaccg
     3121 tgaaacagct caaagaagac tatttcaaaa agattgaatg tttcgactct gttgaaatca
     3181 gcggagtgga ggatcgcttc aacgcatccc tgggaacgta tcacgatctc ctgaaaatca
     3241 ttaaagacaa ggacttcctg gacaatgagg agaacgagga cattcttgag gacattgtcc
     3301 tcacccttac gttgtttgaa gatagggaga tgattgaaga acgcttgaaa acttacgctc
     3361 atctcttcga cgacaaagtc atgaaacagc tcaagaggcg ccgatataca ggatgggggc
     3421 ggctgtcaag aaaactgatc aatgggatcc gagacaagca gagtggaaag acaatcctgg
     3481 attttcttaa gtccgatgga tttgccaacc ggaacttcat gcagttgatc catgatgact
     3541 ctctcacctt taaggaggac atccagaaag cacaagtttc tggccagggg gacagtcttc
     3601 acgagcacat cgctaatctt gcaggtagcc cagctatcaa aaagggaata ctgcagaccg
     3661 ttaaggtcgt ggatgaactc gtcaaagtaa tgggaaggca taagcccgag aatatcgtta
     3721 tcgagatggc ccgagagaac caaactaccc agaagggaca gaagaacagt agggaaagga
     3781 tgaagaggat tgaagagggt ataaaagaac tggggtccca aatccttaag gaacacccag
     3841 ttgaaaacac ccagcttcag aatgagaagc tctacctgta ctacctgcag aacggcaggg
     3901 acatgtacgt ggatcaggaa ctggacatca atcggctctc cgactacgac gtggatcata
     3961 tcgtgcccca gtcttttctc aaagatgatt ctattgataa taaagtgttg acaagatccg
     4021 ataaaaatag agggaagagt gataacgtcc cctcagaaga agttgtcaag aaaatgaaaa
     4081 attattggcg gcagctgctg aacgccaaac tgatcacaca acggaagttc gataatctga
     4141 ctaaggctga acgaggtggc ctgtctgagt tggataaagc aggcttcatc aaaaggcagc
     4201 ttgttgagac acgccagatc accaagcacg tggcccaaat tctcgattca cgcatgaaca
     4261 ccaagtacga tgaaaatgac aaactgattc gagaggtgaa agttattact ctgaagtcta
     4321 agctggtctc agatttcaga aaggactttc agttttataa ggtgagagag atcaacaatt
     4381 accaccatgc gcatgatgcc tacctgaatg cagtggtagg cactgcactt atcaaaaaat
     4441 atcccaagct tgaatctgaa tttgtttacg gagactataa agtgtacgat gttaggaaaa
     4501 tgatcgcaaa gtctgagcag gaaataggca aggccaccgc taagtacttc ttttacagca
     4561 atattatgaa ttttttcaag accgagatta cactggccaa tggagagatt cggaagcgac
     4621 cacttatcga aacaaacgga gaaacaggag aaatcgtgtg ggacaagggt agggatttcg
     4681 cgacagtccg gaaggtcctg tccatgccgc aggtgaacat cgttaaaaag accgaagtac
     4741 agaccggagg cttctccaag gaaagtatcc tcccgaaaag gaacagcgac aagctgatcg
     4801 cacgcaaaaa agattgggac cccaagaaat acggcggatt cgattctcct acagtcgctt
     4861 acagtgtact ggttgtggcc aaagtggaga aagggaagtc taaaaaactc aaaagcgtca
     4921 aggaactgct gggcatcaca atcatggagc gatcaagctt cgaaaaaaac cccatcgact
     4981 ttctcgaggc gaaaggatat aaagaggtca aaaaagacct catcattaag cttcccaagt
     5041 actctctctt tgagcttgaa aacggccgga aacgaatgct cgctagtgcg ggcgagctgc
     5101 agaaaggtaa cgagctggca ctgccctcta aatacgttaa tttcttgtat ctggccagcc
     5161 actatgaaaa gctcaaaggg tctcccgaag ataatgagca gaagcagctg ttcgtggaac
     5221 aacacaaaca ctaccttgat gagatcatcg agcaaataag cgaattctcc aaaagagtga
     5281 tcctcgccga cgctaacctc gataaggtgc tttctgctta caataagcac agggataagc
     5341 ccatcaggga gcaggcagaa aacattatcc acttgtttac tctgaccaac ttgggcgcgc
     5401 ctgcagcctt caagtacttc gacaccacca tagacagaaa gcggtacacc tctacaaagg
     5461 aggtcctgga cgccacactg attcatcagt caattacggg gctctatgaa acaagaatcg
     5521 acctctctca gctcggtgga gacggaggat ccggaccacc aaagaagaag cgtaaggtct
     5581 acccatacga cgtcccagac tacgcctaac cggtatcgat accgtcgact aaagccaaat
     5641 agaaaattat tcagttcctg gcttaagttt ttaaaagtga tattatttat ttggttgtaa
     5701 ccaaccaaaa gaatgtaaat aactaataca taattatgtt agttttaagt tagcaacaaa
     5761 ttgattttag ctatattagc tacttggtta ataaatagaa tatatttatt taaagataat
     5821 tgcgttttta ttgtcaggga gtgagtttgc ttaaaaactc gtttagatcc accgcatggc
     5881 atcattgaaa cagcaaggct cttacaagtt ttcacaaaaa tctatgaact agacaaaaca
     5941 gtaactgcag aaaaaagtcc tatatgtgca aaaaaatgct tgatgatttg taatgagatt
     6001 gaggaggttt cgagacaggc accaaagttt ttacaaatgg atatttccac caccgctcta
     6061 accaatttgt tgaaggaaca cccttggcta tcctttacaa gattcgaact gaagtggaaa
     6121 cagttgtctc ttatcattta tgtattaaga gattttttca ctaattttac ccagaaaaag
     6181 tcacaactag aacaggatca aaatgatcat caaagttatg aagttaaacg atgctccatc
     6241 atgttaagcg atgcagcaca aagaactgtt atgtctgtaa gtagctatat ggacaatcat
     6301 aatgtcaccc catattttgc ctggaattgt tcttattact tgttcaatgc agtcctagta
     6361 cccataaaga ctctactctc aaactcaaaa tcgaatgctg agaataacga gaccgcacaa
     6421 ttattacaac aaattaacac tgttctgatg ctattaaaaa aactggccac ttttaaaatc
     6481 cagacttgtg aaaaatacat tcaagtactg gaagaggtat gtgcgccgtt tctgttatca
     6541 cagtgtgcaa tcccattacc gcatatcagt tataacaata gtaatggtag cgccattaaa
     6601 aatattgtcg gttctgcaac tatcgcccaa taccctactc ttccggagga aaatgtcaac
     6661 aatatcagtg ttaaatatgt ttctcctggc tcagtagggc cttcacctgt gccattgaaa
     6721 tcaggagcaa gtttcagtga tctagtcaag ctgttatcta accgtccacc ctctcgtaac
     6781 tctccagtga caataccaag aagcacacct tcgcatcgct cagtcacgcc ttttctaggg
     6841 caacagcaac agctgcaatc attagtgcca ctgaccccgt ctgctttgtt tggtggcgcc
     6901 aattttaatc aaagtgggaa tattgctgat agctcattgt ccttcacttt cactaacagt
     6961 agcaacggtc cgaacctcat aacaactcaa acaaattctc aagcgctttc acaaccaatt
     7021 gcctcctcta acgttcatga taacttcatg aataatgaaa tcacggctag taaaattgat
     7081 gatggtaata attcaaaacc actgtcacct ggttggacgg accaaactgc gtataacaaa
     7141 gtgaacacgt cgctaagcga aagcccgttt tgtcatcgct ttttgtcggg tctcagttgt
     7201 ggatcgaaaa cccggagagt aaccccttga ccccttgtca gtttccgcat gctccagccc
     7261 ttgtcctcat attttccaat cgtttttggc gtttttagct gcggccattg aatgttcaaa
     7321 aattattgta aacttttcag accccctccg tgtgtgtgtg tgtgtgtgtg cttctgtgta
     7381 tgtggaaaag ttcgaattct agttgggaaa tcaattaaaa gcgcttgttg gactcgcacc
     7441 acaactttcc ctttcgatcg cccctgcggc ttggttcaac tggtcaaggc ctctagctcc
     7501 cgaaaaccgt cgaaaattgg tttggcactt tttatagcca cccaatataa taccaagttc
     7561 agacagaaat tgatatgggt attggactga tcaataactt aagttccctc actgaattat
     7621 ccttaactct tgtgcatgcc tatttatgtg aaaacaatca aaattactca attatgtatg
     7681 cacaggtatt gaaagctccc actatttagt ttttgtttta attatctgtc tttagaactt
     7741 taaatggaaa aacatgactt atatgtatga acaaaaattc caatcgatca ctttaggcgg
     7801 ccgccaatgc caacagaagc attatttttt tcctttcccg atctctcaag cacactcagt
     7861 tgatctcagt cgcaaaactc gttactcact cttaatattt cctcttcttt gcactttcct
     7921 ggtggaatgc attctctatt ttcctctccc tactgcacat actctttgag ctctctagca
     7981 gataccctaa caatttctaa tcatccacga ataataaaca aagatttcta atatcttaaa
     8041 cctgttttat atacttatta ttcaaaaaaa aaagtgtggc aaaaaaaaag agcacttcaa
     8101 cgtttgttga acaaaggata taattttgta agggtatcta gaagttttca tgcctctctt
     8161 catccactct tctcaacctc atgcgatagc tgctgcgctc tctcgtttat cgttcggttg
     8221 aggttttata attctcaaat actttttccc gaagtggtgc ctttaaatag cgtatatgag
     8281 tggaagactt tcgatgtgca gcgtaccaca acgtttaaga gctatgctgg aaacagcata
     8341 gcaagtttaa ataaggctag tccgttatca actttgctgg aaacagcaaa gtggcaccga
     8401 gtcggtgctt tttttgctca cctgtgattg ctcctactca aatacaaaaa catcaaattt
     8461 tctgtcaata aagcatattt atttatattt attttacagg aaagaattcc ttttaaagtg
     8521 tattttaacc tataatgaaa aacgattaaa aaaaatacat aaaataattc gaaaattttt
     8581 gaatagccca ggttgataaa aattcatttc atacgtttta taacttatgc ccctaagtat
     8641 tttttgacca tagtgtttca attctacatt aattttacag agtagaatga aacgccacct
     8701 actcagccaa gaggcgaaaa ggttagctcg ccaagcagag agggcgccag tgctcactac
     8761 tttttataat tctcaacttc tttttccaga ctcagttcgt atatatagac ctattttcaa
     8821 tttaacgtcg tgtattctga gaaagctgga gtttaagagc tatgctggaa acagcatagc
     8881 aagtttaaat aaggctagtc cgttatcaac tttgctggaa acagcaaagt ggcaccgagt
     8941 cggtgctttt tttgcctacc tggagcctga gagttgttca ataaaataaa aatgtttcgt
     9001 ttttttgctt tcgccagtat ttattatttt tcatcaatat gtattcaatt tggtatgtat
     9061 ttacgcgtgg cttgctgttc ttcgcgttca aaatctccag ctccattttg ctttcggtgc
     9121 gcttgcaatc agtactgtcc aaaatcgaaa atcgccgaac cgtagtgtga ccgtgcgggg
     9181 ctctgcgaaa ataaactttt ttaggtatat ggccacacac ggggaaagca cagtggatta
     9241 tatgtattaa tatattatgc aggttttcat tacttatcca gatgtaagcc cacttaaagc
     9301 gatttaacaa ttatttgccg aaagagtata aacaaatttc acataaaaat ggattaagaa
     9361 aagcttgtgt aagattatgc gcagcgttgc cagatagctc catttaaaac acttcaaaaa
     9421 caataagttt agaaaatata tacataaata gcagtcgttg ccgcaacgct caacacatca
     9481 cacttttaaa acacccttta cctacacaga attacttttt aaatttccag tcaagctgcg
     9541 agtttcaaaa ttatagccgg tagagaagac agtgctattt caaaagcaaa ctaacaaggg
     9601 tcttaaattt caaaacacca atcctaacaa gccttggact tttgtaagtt tagatcaaag
     9661 gtggcattgc attcaatgtc atggtaagaa gtaggtcgtc taggtagaaa tcctcattca
     9721 gccggtcaag tcagtacgag aaaggtctca atttgaaatt gtcttaaaaa tattttattg
     9781 ttttgtactg tggtgagttt aaacgaaaaa cacaaaaaaa aagtgataca cagaaatcat
     9841 aaaaaatttt aatacaaggt attcgtacgt atcaaaaaca tttcggcaca attttttttc
     9901 tctgtactaa agtgttacga acactacggt attttttagt gattttcaac ggacaccgaa
     9961 ggtatataaa cagcgttcgc gaacggtcgc cttcaaaacc aattgacatt tgcagcagca
    10021 agtacaagta gaaagtaaag cgcaatcagc gaaaaattta tacttaattg ttggtgatta
    10081 aagtacaatt aaaagaacat tctcgaaagt cacaagaaac gtaagttttt aactcgctgt
    10141 taccaattag taataagagc aacaagacgt tgagtaattt caagaaaaac tgcatttcaa
    10201 ggtctttgtt cggccatttt ttttttattc aacgctctac gtaattacaa aataagaaat
    10261 tggcagccac gcatcttgtt ttcccaatga attggcatca aaacgcaaac aaatctataa
    10321 ataaaacttg cgtgttgatt ttcgccaaga tttattggca aattgtgaaa ttcgcagtga
    10381 cgcatttgaa aattcgagaa atcacgaacg cactcgagca tttgtgtgca tgttattagt
    10441 tagttgttta gttaattgaa gtattttacc aacgaaatcc acttattttt agctgaaata
    10501 gagtaggttg cttaaacaaa gccacgtctg aaaatttctt attgcttgta gttgtgacgt
    10561 caccatatac acacaaaata atgtgtatgc atgcgtttca gctgtgtata tatacatgca
    10621 cacactcgca ttatgaaaac gatgacgagc aacggaacag gtttctcaac tacctttgtt
    10681 cctgtttctt cgctttcctt tgttccaata ttcgtagagg gttaataggg gtttctcaac
    10741 aaagttggcg tcgataaata agtttcccat ttttattccc cagccaggaa gttagtttca
    10801 atagttttgt aatttcaacg aaactcattt gatttcgtac taattttcca catctctatt
    10861 ttctgcccgc agaataatcc aactgaatag ggaattggga acttaaaaaa aaaaatcaaa
    10921 atgagcggta ccgatcgcag cccccccaag aagaagcgca aggtggagtt cggatccggt
    10981 ggcggcggaa gtggaggtgg aggctcgatg gtgtccaagg gcgaggagct gatcaaggag
    11041 aacatgcaca tgaagctgta catggagggc accgtggaca accaccactt caagtgcacc
    11101 tccgagggcg agggcaagcc ctacgagggc acccagacca tgcgcatcaa ggtggtggag
    11161 ggcggccccc tgcccttcgc cttcgacatc ctggccacct ccttcctgta cggctccaag
    11221 accttcatca accacaccca gggcatcccc gacttcttca agcagtcctt ccccgagggc
    11281 ttcacctggg agcgcgtgac cacctacgag gacggcggcg tgctgaccgc cacccaggac
    11341 acctccctgc aagacggctg cctgatctac aacgtgaaga tccgcggcgt gaacttcacc
    11401 tccaacggcc ccgtgatgca gaagaagacc ctgggctggg aggccttcac cgagaccctg
    11461 taccccgccg acggcggcct ggagggccgc aacgacatgg ccctgaagct ggtgggcggc
    11521 tcccacctga tcgccaacgc caagaccacc taccgctcca agaagcccgc caagaacctg
    11581 aagatgcccg gcgtgtacta cgtggactac cgcctggagc gcatcaagga ggccaacaac
    11641 gagacctacg tggagcagca cgaggtggcc gtggcccgct actgcgacct gccctccaag
    11701 ctgggccaca agctgaacta atctagaatt aattaactaa gccagtcggc aatcggacgc
    11761 cttcgaaaca tgcaacacta atgtttaatt cagatttcag cagagacaag ctaaaacacc
    11821 gacgagttgt aatcatttct gtgcgccagc atatatttct tatatacaac gtaatacata
    11881 attatgtaat tctagcatct ccccaacact cacatacaaa caaacaaaaa atacaaacac
    11941 acaaaacgta tttacccgca cgcatccttg gcgaggttga gtatgaaaca aaaacaaaac
    12001 ttaatttaga gcaaagtaat tacacgaata aatttaataa aaaaaaacta taataaaaac
    12061 gccaatcatg ttatttccaa aaaaaaaaaa aaaaaaacta gacgagatgt ttgcgcttgt
    12121 gtcgatgaat gtaaagaatt gcaactacat agatggtaat taatttcagc tgtaagaagt
    12181 acaagttttt cataattgtg agaaaagtgc agtagtatcg cttaaagttc agttttaatc
    12241 agggcattta gcagctcgaa cccaatgtat ttgtaagccc aataaacgtg taaatgccta
    12301 gagcaaaata tcacaactgt agcttttcat ccgaaagata ataatgaata gactgacctg
    12361 acgtttttca aaatgcgact agtggcctaa ttctagtatg tatgtaagtt aataaaaccc
    12421 atttttgcgg aaagtagata aaaaaaacat tttttttttt tactgcactg gatatcattg
    12481 aacttatctg atcagtttta aatttacttc gatccaaggg tatttgatgt accaggttct
    12541 ttcgattacc tctcactcaa aatgacattc cactcaaagt cagcgctgtt tgcctccttc
    12601 tctgtccaca gaaatatcgc cgtctctttc gccgctgcgt ccgctatctc tttcgccacc
    12661 gtttgtagcg ttacgtagcg tcaatgtccg ccttcagttg cattttgtca gcggtttcgt
    12721 gacgaagctc caagcggttt acgccatcaa ttaaacacaa agtgctgtgc caaaactcct
    12781 ctcgcttctt atttttgttt gttttttgag tgattggggt ggtgattggt tttgggtggg
    12841 taagcagggg aaagtgtgaa aaatcccggc aatgggccaa gaggatcagg agctattaat
    12901 tcgcggaggc agcaaacacc catctgccga gcatctgaac aatgtgagta gtacatgtgc
    12961 atacatctta agttcacttg atctatagga actgcgattg caacatcaaa ttgtctgcgg
    13021 cgtgagaact gcgacccaca aaaatcccaa accgcaattg cacaaacaaa tagtgacacg
    13081 aaacagatta ttctggtagc tgttctcgct atataagaca atttttgaga tcatatcatg
    13141 atcaagacat ctaaaggcat tcattttcga ctatattctt ttttacaaaa aatataacaa
    13201 ccagatattt taagctgatc ctagatgcac aaaaaataaa taaaagtata aacctacttc
    13261 gtaggatact tcggggtact ttttgttcgg ggttagatga gcataacgct tgtagttgat
    13321 atttgagatc ccctatcatt gcagggtgac agcggagcgg cttcgcagag ctgcattaac
    13381 cagggcttcg ggcaggccaa aaactacggc acgctccggc cacccagtcc gccggaggac
    13441 tccggttcag ggagcggcca actagccgag aacctcacct atgcctggca caatatggac
    13501 atctttgggg cggtcaatca gccgggctcc ggatggcggc agctggtcaa ccggacacgc
    13561 ggactattct gcaacgagcg acacataccg gcgcccagga aacatttgct caagaacggt
    13621 gagtttctat tcgcagtcgg ctgatctgtg tgaaatctta ataaagggtc caattaccaa
    13681 tttgaaactc agtttgcggc gtggcctatc cgggcgaact tttggccgtg atgggcagtt
    13741 ccggtgccgg aaagacgacc ctgctgaatg cccttgcctt tcgatcgccg cagggcatcc
    13801 aagtatcgcc atccgggatg cgactgctca atggccaacc tgtggacgcc aaggagatgc
    13861 aggccaggtg cgcctatgtc cagcaggatg acctctttat cggctcccta acggccaggg
    13921 aacacctgat tttccaagcc atggtgcgga tgccacgaca tctgacctat cggcagcgag
    13981 tggcccgcgt ggatcaggtg atccaggagc tttcgctcag caaatgtcag cacacgatca
    14041 tcggtgtgcc cggcagggtg aaaggtctgt ccggcggaga aaggaagcgt ctggcattcg
    14101 cctccgaggc tctaaccgat ccgccgcttc tgatctgcga tgagcccacc tccggactgg
    14161 actcctttac cgcccacagc gtcgtccagg tgctgaagaa gctgtcgcag aagggcaaga
    14221 ccgtcatcct gaccattcat cagccgtctt ccgagctgtt tgagctcttt gacaagatcc
    14281 ttctgatggc cgagggcagg gtagctttct tgggcactcc cagcgaagcc gtcgacttct
    14341 tttcctagtg agttcgatgt gtttattaag ggtatctagt attacataac atctcaactc
    14401 ctatccagcg tgggtgccca gtgtcctacc aactacaatc cggcggactt ttacgtacag
    14461 gtgttggccg ttgtgcccgg acgggagatc gagtcccgtg atcggatcgc caagatatgc
    14521 gacaattttg ccattagcaa agtagcccgg gatatggagc agttgttggc caccaaaaat
    14581 ctggagaagc cactggagca gccggagaat gggtacacct acaaggccac ctggttcatg
    14641 cagttccggg cggtcctgtg gcgatcctgg ctgtcggtgc tcaaggaacc actcctcgta
    14701 aaagtgcgac ttattcagac aacggtgagt ggttccagtg gaaacaaatg atataacgct
    14761 tacaattctt ggaaacaaat tcgctagatt ttagatagaa ttgcctgatt ccacaccctt
    14821 cttagttttt ttcaatgaga tgtatagttt atagttttgc agaagataaa taaatttcat
    14881 ttaactcgcg aatattaatg agatgcgagt aacattttaa tttgcagatg gttgccatct
    14941 tgattggcct catctttttg ggccaacaac tcacgcaagt gggtgtgatg aatatcaacg
    15001 gagccatctt cctcttcctg accaacatga cctttcaaaa cgtctttgcc acgataaatg
    15061 taagtcatgt ttagaataca tttgcatttc aataatttac taactttcta atgaatcgat
    15121 tcgatttagg tgttcacctc agagctgcca gtttttatga gggaggcccg aagtcgactt
    15181 tatcgctgtg acacatactt tctgggcaaa acgattgccg aattgccgct ttttctcaca
    15241 gtgccactgg tcttcacggc gattgcctat ccgatgatcg gactgcgggc cggagtgctg
    15301 cacttcttca actgcctggc gctggtcact ctggtggcca atgtgtcaac gtccttcgga
    15361 tatctaatat cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt
    15421 atcataccat tcctgctctt tggcggcttc ttcttgaact cgggctcggt gccagtatac
    15481 ctcaaatggt tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac
    15541 caatgggcgg acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc
    15601 agttcgggca aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac
    15661 tacgtgggtc tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga
    15721 cttcgggccc gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt
    15781 tttaccatta ttaccatcgt gtttactgtt tattgccccc tcaaaaagct aatgtaatta
    15841 tatttgtgcc aataaaaaca agatatgacc tatagaatac aagtatttcc ccttcgaaca
    15901 tccccacaag tagactttgg atttgtcttc taaccaaaag acttacacac ctgcatacct
    15961 tacatcaaaa actcgtttat cgctacataa aacaccggga tatatttttt atatacatac
    16021 ttttcaaatc gcgcgccctc ttcataattc acctccacca caccacgttt cgtagttgct
    16081 ctttcgctgt ctcccacccg ctctccgcaa cacattcacc ttttgttcga cgaccttgga
    16141 gcgactgtcg ttagttccgc gcgattcggt tcgctcaaat ggttccgagt ggttcatttc
    16201 gtctcaatag aaattagtaa taaatatttg tatgtacaat ttatttgctc caatatattt
    16261 gtatatattt ccctcacagc tatatttatt ctaatttaat attatgactt tttaaggtaa
    16321 ttttttgtga cctgttcgga gtgattagcg ttacaatttg aactgaaagt gacatccagt
    16381 gtttgttcct tgtgtagatg catctcaaaa aaatggtggg cataatagtg ttgtttatat
    16441 atatcaaaaa taacaactat aataataaga atacatttaa tttagaaaat gcttggattt
    16501 cactggaact agaattaatt cggctgctgc tctaaacgac gcatttcgta ctccaaagta
    16561 cgaatttttt ccctcaagct cttattttca ttaaacaatg aacaggacct aacgcacagt
    16621 cacgttattg tttacataaa tgattttttt tactattcaa acttactctg tttgtgtact
    16681 cccactggta tagccttctt ttatcttttc tggttcaggc tctatcactt tactaggtac
    16741 ggcatctgcg ttgagtcgcc tccttttaaa tgtctgacct tttgcaggtg cagccttcca
    16801 ctgcgaatca ttaaagtggg tatcacaaat ttgggagttt tcaccaaggc tgcacccaag
    16861 gctctgctcc cacaattttc tcttaatagc acacttcggc acgtgaatta attttactcc
    16921 agtcacagct ttgcagcaaa atttgcaata tttcattttt ttttattcca cgtaagggtt
    16981 aatgttttca aaaaaaaatt cgtccgcaca caacctttcc tctcaacaag caaacgtgca
    17041 ctgaatttaa gtgtatactt cggtaagctt cggctatcga cgggaccacc ttatgttatt
    17101 tcatcatggg ccagacccac gtagtccagc ggcagatcgg cggcggagaa gttaagcgtc
    17161 tccaggatga ccttgcccga actggggcac gtggtgttcg acgatgtgca gctaatttcg
    17221 cccggctcca cgtccgccca ttggttaatc agcagaccct cgttggcgta acggaaccat
    17281 gagaggtacg acaaccattt gaggtatact ggcaccgagc ccgagttcaa gaagaaggcg
    17341 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
    17401 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
    17461 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
    17521 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
    17581 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    17641 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    17701 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    17761 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
    17821 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    17881 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    17941 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    18001 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
    18061 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
    18121 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
    18181 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
    18241 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    18301 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    18361 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
    18421 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    18481 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    18541 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    18601 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    18661 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    18721 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    18781 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    18841 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    18901 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    18961 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    19021 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    19081 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
    19141 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
    19201 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
    19261 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
    19321 gagcagattg tactgagagt gcaccatatc gacatgcccg ccgtgaccgt cgagaacccg
    19381 ctgacgctgc cccgcgtatc cgcacccgcc gacgccgtcg cacgtcccgt gctcaccgtg
    19441 accaccgcgc ccagcggttt cgagggcgag ggcttcccgg tgcgccgcgc gttcgccggg
    19501 atcaactacc gccacctcga cccgttcatc atgatggacc agatgggtga ggtggagtac
    19561 gcgcccgggg agcccaaggg cacgccctgg cacccgcacc gcggcttcga gaccgtgacc
    19621 tacatcgtcg atatgcggtg tgaaataccg caccgaatcg cgcggaacta acgacagtcg
    19681 ctccaaggtc gtcgaacaaa aggtgaatgt gttgcggaga gcgggtggga gacagcgaaa
    19741 gagcaactac gaaacgtggt gtggtggagg tgaattatga agagggcgcg cgatttgaaa
    19801 agtatgtata taaaaaatat atcccggtgt tttatgtagc gataaacgag tttttgatgt
    19861 aaggtatgca ggtgtgtaag tcttttggtt agaagacaaa tccaaagtct acttgtgggg
    19921 atgttcgaag gggaaatact tgtattctat aggtcatatc ttgtttttat tggcacaaat
    19981 ataattacat tagctttttg agggggcaat aaacagtaaa cacgatggta ataatggtaa
    20041 aaaaaaaaaa caagcagtta tttcggatat atgtcggcta ctccttgcgt cgggcccgaa
    20101 gtcttagagc cagatatgcg agcacccgga agctcacgat gagaatggcc agac
//
