LOCUS       sequence-381831-        5486 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synDHBR Cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5486)
  AUTHORS   Sanford A, Kiriakov S, Khalil AS
  TITLE     A Toolkit for Precise, Multigene Control in Saccharomyces 
            cerevisiae.
  JOURNAL   ACS Synth Biol. 2022 Nov 11. doi: 10.1021/acssynbio.2c00423.
  PUBMED    36367334
REFERENCE   2  (bases 1 to 5486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2022 Nov 11. doi: 10.1021/acssynbio.2c00423."
COMMENT     Sequence Label: pAS160
FEATURES             Location/Qualifiers
     source          1..5486
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        468..881
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             2623..3192
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     terminator      3215..3402
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3270..3294)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(join(3719..4510,4511..4579))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4342..4361
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4580..4684)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4787..5375
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5276..5295
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaattcgcat ctagaacggg gtcatcacgg ctcatcatgc gccaaacaaa tgtgtgcaat
       61 acacgctcgg atgactgcat gatgaccgca ctgactgggg acagcagatc cacctaagcc
      121 tgtgagagaa gcagacaccc gacagatcaa ggcagttacg tctcaccaaa ccagatgtca
      181 acacagctac aacgccttgc caacagggag ttcttcagag acatggaggc tcaaaacgaa
      241 attattgaca gcctagacat caatagtcat acaacagaaa gcgaccaccc aactttggct
      301 gataatagcg tataaacaat gcatactttg tacgttcaaa atacaatgca gtagatatat
      361 ttatgcatat tacatataat acatatcaca taggaagcaa caggcgcgtt ggacttttaa
      421 ttttcgagga ccgcgaatcc ttacatcaca cccaatcccc cacaagtgat cccccacaca
      481 ccatagcttc aaaatgtttc tactcctttt ttactcttcc agattttctc ggactccgcg
      541 catcgccgta ccacttcaaa acacccaagc acagcatact aaatttcccc tctttcttcc
      601 tctagggtgt cgttaattac ccgtactaaa ggtttggaaa agaaaaaaga caccgcctcg
      661 tttctttttc ttcgtcgaaa aaggcaataa aaatttttat cacgtttctt tttcttgaaa
      721 attttttttt ttgatttttt tctctttcga tgacctccca ttgatattta agttaataaa
      781 cggtcatcaa tttctcaagt ttcagtttca tttttcttgt tctattacaa ctttttttac
      841 ttcttgctca ttagaaagaa agcatagcaa tctaatctaa gttttaatta caaaagatct
      901 atgctcgaga aaatggagcg acccttccag tgcaggattt gcatgcggaa cttttcgaga
      961 caatctaatt tgtcaagaca tacaaggact cataccggtg aaaaaccgtt tcagtgtagg
     1021 atctgtatgc gaaatttcag tagaaatgaa catttggttc tacatctacg tacgcacaca
     1081 ggcgaaaagc cattccaatg ccgaatatgc atgcgcaact tcagtcaaaa aactggtctt
     1141 agagttcatc taaaaaccca ccttagagga gatggtgctg gattaattga tatcgatatg
     1201 ggtacaagac catatgcttg tccagttgag tcttgtgaca gaagattctc aagaagcgac
     1261 gaattaacgc gtcacattag aattcataca ggtcaaaaac cttttcagtg tagaatctgc
     1321 atgagaaact tttcacgttc ggatcacctt actactcata tcagaacgca cactggtgaa
     1381 aaaccttttg catgtgatat ttgtggaaga aaattcgcaa gatctgatga aagaaagcgt
     1441 cacacaaaaa tccacactgg tgggggcgga actccagctg ctgcttccac cttggaagat
     1501 cctagtgctg gggatatgcg tgctgctaac ttgtggcctt ctccattaat gataaaaaga
     1561 tctaaaaaga attctcttgc tttgtcgttg actgcagatc aaatggtttc tgctttatta
     1621 gacgccgaac ctcctatttt atactctgaa tatgatccta ctaggccatt ttcggaagcc
     1681 tctatgatgg gtttaattac caatttgatg gatagagaat tagtgcatat gattaattgg
     1741 gccaagaggg tccctggttt tgttgattta accttacacg atcaagtaca tttattagaa
     1801 tgtgcttggc ttgaaatctt gcaaattggt ctagtttgga gatccatgga acatccagtt
     1861 aaattactat tcgctcctaa tttgcttttg gacagaaacc aaggtaagtg tgtagaagga
     1921 atggttgaaa tttttgatat gctacttgct acaagctcaa gattcagaat gatgaacttg
     1981 caaggtgagg aattcgtttg tttgaaatcg attatactac tcaattctgg agtttacact
     2041 ttcttgtcat ccacgttaaa gtctttagaa gaaaaggacc acatacatag ggtccttgac
     2101 aagatcactg acacattaat tcatttaatg gccaaggctg gtttaacctt gcaacaacaa
     2161 caccaacgtt tggcccaatt gttgttgata ttgagtcaca tacgtcatat gtctaataaa
     2221 tctatggaac acctagactc catgaagtgt aagaacgtcg tacccctata cgacttattg
     2281 ttagaaatgt tggatgcaca tagattacat gcccctacta gtagaggtgg agcatcagta
     2341 gaggaaactg atcaatcgca tttggctact gcaggttcca ctagttccga attacattta
     2401 gatggtgagg atgtagctat ggcccacgca gatgcgctag atgattttga tctagacatg
     2461 ctcggtgacg gggactcccc tggtccgggg tttaccccac atgattccgc cccatatggc
     2521 gctttggata tggctgactt tgaattcgaa caaatgttta ctgatgcatt gggtatcgat
     2581 gaatatggtg gtggcgacgg agcaggctta ataaatgcta gcagggattc cagggaaggg
     2641 atgtttttgc cgaaacctga agcgggctca gcgattagtg acgtgtttga gggccgcgag
     2701 gtgtgccagc caaaacgaat aaggccattt catcctccag gaagtccatg ggccaatcgc
     2761 ccactccccg caagcctcgc accaacacca accggtccag tacatgagcc agtcgggtca
     2821 ctgacaccgg caccagtccc tcagccactg gatccagcgc ccgcagtgac tcccgaggcg
     2881 agtcacctat tggaggatcc cgatgaagaa acaagccagg ctgtcaaagc ccttcgggag
     2941 atggccgata cggtgattcc tcagaaagaa gaagcggcaa tttgtggcca aatggacctt
     3001 tcacatccgc cccctagggg ccatctggat gaactgacaa ccacacttga gtcaatgacc
     3061 gaggatctga acctggactc acccctgacg ccggaactta atgagattct ggatacattc
     3121 ctgaacgacg agtgcctctt acatgcaatg catataagca caggactatc aatctttgac
     3181 acatctctgt tttaagggcc catcctaact cgaggcgaat ttcttatgat ttatgatttt
     3241 tattattaaa taagttataa aaaaaataag tgtatacaaa ttttaaagtg actcttaggt
     3301 tttaaaacga aaattcttat tcttgagtaa ctctttcctg taggtcaggt tgctttctca
     3361 ggtatagcat gaggtcgctc ttattgacca cacctctacc ggcatgccga gcaaatgcct
     3421 gcaaatcgct ccccatttcg ctgagcatga gacggaaatc tgctcgtcag tggtgctcac
     3481 actgacgaat catgtacaga tcataccgat gactgcctgg cgactcacaa ctaagcaaga
     3541 cagccggaac cagcgccggc gaacaccact gcatatatgg catatcacaa cagtccaact
     3601 agtgcactgc agtacagcgg ccgcgattat caaaaaggat cttcacctag atccttttaa
     3661 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     3721 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     3781 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     3841 gtgctgcaat gataccgcgg gacccacgct caccggctcc agatttatca gcaataaacc
     3901 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     3961 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     4021 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     4081 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     4141 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     4201 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     4261 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     4321 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     4381 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     4441 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     4501 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     4561 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     4621 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     4681 cgcgcacatt tccccgaaaa gtgccacctg tcatgaccaa aatcccttaa cgtgagtttt
     4741 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     4801 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     4861 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     4921 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     4981 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     5041 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     5101 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     5161 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     5221 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     5281 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     5341 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     5401 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     5461 ctgtggataa ccgtgcggcc gcccct
//
