LOCUS       sequence-381833-       17256 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To insert T2A-Flp1 into Gal4 coding sequence in Drosophila, 
            converting Gal4 into a T2A-Flp1 transgene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17256)
  AUTHORS   Karuparti S, Yeung AT, Wang B, Guicardi PF, Han C
  TITLE     A toolkit for converting Gal4 into LexA and Flippase transgenes in 
            Drosophila.
  JOURNAL   G3 (Bethesda). 2023 Mar 9;13(3):jkad003. doi: 
            10.1093/g3journal/jkad003.
  PUBMED    36617215
REFERENCE   2  (bases 1 to 17256)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-03-9"; volume: "13(3):jkad003. doi"; 
            pages: " 10.1093/g3journal/jkad003"
COMMENT     Sequence Label: pHACK(Gal4)-DONR(T2A-Flp1)
FEATURES             Location/Qualifiers
     source          1..17256
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(1..31,32..233)
                     /label=P element 3' end
                     /note="P element 3' end"
     CDS             246..686
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             1368..1421
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     promoter        5512..5932
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     CDS             8047..8067
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(9504..9522)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(9974..10045,10447..10720,10795..11449,11511..11826,
                     12030..12161,12232..12846)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(13625..14179,14180..14210)
                     /label=P element 5' end
     rep_origin      complement(14445..15033)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14525..14544)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15204..15995,15996..16064))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15827..15846
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16065..16169)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     16237..16255
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(16293..16315)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     16415..16434
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    16644..16713
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
       61 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
      121 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
      181 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
      241 gctgcatgaa gctactgtct tctatcgaac aagcatgcga tatttgccga cttaaaaagc
      301 tcaagtgctc caaagaaaaa ccgaagtgcg ccaagtgtct gaagaacaac tgggagtgtc
      361 gctactctcc caaaaccaaa aggtctccgc tgactagggc acatctgaca gaagtggaat
      421 caaggctaga aagactggaa cagctatttc tactgatttt tcctcgagaa gaccttgaca
      481 tgattttgaa aatggattct ttacaggata taaaagcatt gttaacagga ttatttgtac
      541 aagataatgt gaataaagat gccgtcacag atagattggc ttcagtggag actgatatgc
      601 ctctaacatt gagacagcat agaataagtg cgacatcatc atcggaagag agtagtaaca
      661 aaggtcaaag acagttgact gtatcgattg actcggcagc tcatcatgat aactccacaa
      721 ttccgttgga ttttatgccc agggatgctc ttcatggatt tgattggtct gaagaggatg
      781 acatgtcgga tggcttgccc ttcctgaaaa cggaccccaa caataatggg ttctttggcg
      841 acggttctct cttatgtatt cttcgatcta ttggctttaa accggaaaat tacacgaact
      901 ctaacgttaa caggctcccg accatgatta cggatagata cacgttggct tctagatcca
      961 caacatcccg tttacttcaa agttatctca ataattttca cccctactgc cctatcgtgc
     1021 actcaccgac gctaatgatg ttgtataata accagattga aatcgcgtcg aaggatcaat
     1081 ggcaaatcct ttttaactgc atattagcca ttggagcctg gtgtatagag ggggaatcta
     1141 ctgatataga tgttttttac tatcaaaatg ctaaatctca tttgacgagc aaggtcttcg
     1201 agtcaggttc cataattttg gtgacagccc tacatcttct gtcgcgatat acacagtgga
     1261 ggcagaaaac aaatactagc tataattttc acagcttttc cataagaatg gccatatcat
     1321 tgggcttgaa tagggacctc ccctcgtcct tcagtgatag cagcggagag ggcagaggaa
     1381 gtctgctaac atgcggtgac gtcgaggaga atcctggccc aggaagcgga ggcgctagca
     1441 tgccgcagtt tgatatcctc tgcaagaccc caccaaaggt gttggtgcgt caattcgtgg
     1501 agcgatttga gaggccgtcg ggtgagaaga tcgccctgtg cgctgccgag ttgacctatt
     1561 tgtgttggat gatcactcat aatggcaccg cgattaagcg cgctaccttt atgagctata
     1621 acactatcat tagcaattcc ctgtcctttg acatagtaaa caagtccctg cagtttaaat
     1681 acaagactca gaaggccact atattggagg cttcgctgaa aaagttgatc ccggcatggg
     1741 agttcacgat catcccatac tacggtcaga aacaccagag cgatattacc gatattgtaa
     1801 gcagcctcca gctgcagttt gagtccagcg aagaggctga taagggtaat agtcacagca
     1861 aaaagatgct gaaggcactg ctgtccgagg gcgaaagcat ctgggagatt actgagaaaa
     1921 tcctgaactc gtttgagtac accagccgat tcaccaagac gaagaccctg taccagttcc
     1981 tctttttggc aaccttcatc aattgtggtc gcttcagtga catcaaaaac gtggacccta
     2041 aatcgttcaa gctggtgcag aataagtacc tgggtgttat catccagtgc ttggttaccg
     2101 agactaagac ctccgtgtcc aggcacattt atttcttctc cgcaaggggt cgcatcgatc
     2161 cgctggtata cttggatgag ttccttcgga atagtgaacc agtcttgaag cgcgtgaaca
     2221 ggacgggcaa ttccagtagc aacaagcaag agtaccagct gctgaaggat aatcttgttc
     2281 ggtcgtacaa caaagccttg aagaaaaatg cgccatactc gatctttgca atcaagaatg
     2341 gtcccaagag tcatattggc cggcacctga tgaccagttt cttgtccatg aagggtctga
     2401 ctgagctcac aaacgtcgtt ggcaactggt cggacaagcg agcgagtgcc gtcgcccgca
     2461 cgacatacac ccaccagatc acggccatac cagatcacta tttcgcgctg gtgtcgcgtt
     2521 attatgccta tgatcccatc agcaaggaga tgatcgcgct gaaggacgaa accaatccaa
     2581 tcgaggagtg gcagcatatc gagcaactta agggaagcgc tgagggtagc atccgttacc
     2641 ccgcctggaa cggcatcatc agccaggagg ttctggatta cctgagctcc tacatcaatc
     2701 gccgtattta accggtatcg ataccgtcga ctaaagccaa atagaaaatt attcagttcc
     2761 tggcttaagt ttttaaaagt gatattattt atttggttgt aaccaaccaa aagaatgtaa
     2821 ataactaata cataattatg ttagttttaa gttagcaaca aattgatttt agctatatta
     2881 gctacttggt taataaatag aatatattta tttaaagata attgcgtttt tattgtcagg
     2941 gagtgagttt gcttaaaaac tcgtttagat ccaccgcatg gcatcattga aacagcaagg
     3001 ctcttacaag ttttcacaaa aatctatgaa ctagacaaaa cagtaactgc agaaaaaagt
     3061 cctatatgtg caaaaaaatg cttgatgatt tgtaatgaga ttgaggaggt ttcgagacag
     3121 gcaccaaagt ttttacaaat ggatatttcc accaccgctc taaccaattt gttgaaggaa
     3181 cacccttggc tatcctttac aagattcgaa ctgaagtgga aacagttgtc tcttatcatt
     3241 tatgtattaa gagatttttt cactaatttt acccagaaaa agtcacaact agaacaggat
     3301 caaaatgatc atcaaagtta tgaagttaaa cgatgctcca tcatgttaag cgatgcagca
     3361 caaagaactg ttatgtctgt aagtagctat atggacaatc ataatgtcac cccatatttt
     3421 gcctggaatt gttcttatta cttgttcaat gcagtcctag tacccataaa gactctactc
     3481 tcaaactcaa aatcgaatgc tgagaataac gagaccgcac aattattaca acaaattaac
     3541 actgttctga tgctattaaa aaaactggcc acttttaaaa tccagacttg tgaaaaatac
     3601 attcaagtac tggaagaggt atgtgcgccg tttctgttat cacagtgtgc aatcccatta
     3661 ccgcatatca gttataacaa tagtaatggt agcgccatta aaaatattgt cggttctgca
     3721 actatcgccc aataccctac tcttccggag gaaaatgtca acaatatcag tgttaaatat
     3781 gtttctcctg gctcagtagg gccttcacct gtgccattga aatcaggagc aagtttcagt
     3841 gatctagtca agctgttatc taaccgtcca ccctctcgta actctccagt gacaatacca
     3901 agaagcacac cttcgcatcg ctcagtcacg ccttttctag ggcaacagca acagctgcaa
     3961 tcattagtgc cactgacccc gtctgctttg tttggtggcg ccaattttaa tcaaagtggg
     4021 aatattgctg atagctcatt gtccttcact ttcactaaca gtagcaacgg tccgaacctc
     4081 ataacaactc aaacaaattc tcaagcgctt tcacaaccaa ttgcctcctc taacgttcat
     4141 gataacttca tgaataatga aatcacggct agtaaaattg atgatggtaa taattcaaaa
     4201 ccactgtcac ctggttggac ggaccaaact gcgtataaca aagtgaacac gtcgctaagc
     4261 gaaagcccgt tttgtcatcg ctttttgtcg ggtctcagtt gtggatcgaa aacccggaga
     4321 gtaacccctt gaccccttgt cagtttccgc atgctccagc ccttgtcctc atattttcca
     4381 atcgtttttg gcgtttttag ctgcggccat tgaatgttca aaaattattg taaacttttc
     4441 agaccccctc cgtgtgtgtg tgtgtgtgtg tgcttctgtg tatgtggaaa agttcgaatt
     4501 ctagttggga aatcaattaa aagcgcttgt tggactcgca ccacaacttt ccctttcgat
     4561 cgcccctgcg gcttggttca actggtcaag gcctctagct cccgaaaacc gtcgaaaatt
     4621 ggtttggcac tttttatagc cacccaatat aataccaagt tcagacagaa attgatatgg
     4681 gtattggact gatcaataac ttaagttccc tcactgaatt atccttaact cttgtgcatg
     4741 cctatttatg tgaaaacaat caaaattact caattatgta tgcacaggta ttgaaagctc
     4801 ccactattta gtttttgttt taattatctg tctttagaac tttaaatgga aaaacatgac
     4861 ttatatgtat gaacaaaaat tccaatcgat cactttaggc ggccgccaat gccaacagaa
     4921 gcattatttt tttcctttcc cgatctctca agcacactca gttgatctca gtcgcaaaac
     4981 tcgttactca ctcttaatat ttcctcttct ttgcactttc ctggtggaat gcattctcta
     5041 ttttcctctc cctactgcac atactctttg agctctctag cagataccct aacaatttct
     5101 aatcatccac gaataataaa caaagatttc taatatctta aacctgtttt atatacttat
     5161 tattcaaaaa aaaaagtgtg gcaaaaaaaa agagcacttc aacgtttgtt gaacaaagga
     5221 tataattttg taagggtatc tagaagtttt catgcctctc ttcatccact cttctcaacc
     5281 tcatgcgata gctgctgcgc tctctcgttt atcgttcggt tgaggtttta taattctcaa
     5341 atactttttc ccgaagtggt gcctttaaat agcgtatatg agtggaagac tttcgatgtg
     5401 cagcgtacca caacgtttaa gagctatgct ggaaacagca tagcaagttt aaataaggct
     5461 agtccgttat caactttgct ggaaacagca aagtggcacc gagtcggtgc tttttttgct
     5521 cacctgtgat tgctcctact caaatacaaa aacatcaaat tttctgtcaa taaagcatat
     5581 ttatttatat ttattttaca ggaaagaatt ccttttaaag tgtattttaa cctataatga
     5641 aaaacgatta aaaaaaatac ataaaataat tcgaaaattt ttgaatagcc caggttgata
     5701 aaaattcatt tcatacgttt tataacttat gcccctaagt attttttgac catagtgttt
     5761 caattctaca ttaattttac agagtagaat gaaacgccac ctactcagcc aagaggcgaa
     5821 aaggttagct cgccaagcag agagggcgcc agtgctcact actttttata attctcaact
     5881 tctttttcca gactcagttc gtatatatag acctattttc aatttaacgt cgtgtattct
     5941 gagaaagctg gagtttaaga gctatgctgg aaacagcata gcaagtttaa ataaggctag
     6001 tccgttatca actttgctgg aaacagcaaa gtggcaccga gtcggtgctt tttttgccta
     6061 cctggagcct gagagttgtt caataaaata aaaatgtttc gtttttttgc tttcgccagt
     6121 atttattatt tttcatcaat atgtattcaa tttggtatgt atttacgcgt ggcttgctgt
     6181 tcttcgcgtt caaaatctcc agctccattt tgctttcggt gcgcttgcaa tcagtactgt
     6241 ccaaaatcga aaatcgccga accgtagtgt gaccgtgcgg ggctctgcga aaataaactt
     6301 ttttaggtat atggccacac acggggaaag cacagtggat tatatgtatt aatatattat
     6361 gcaggttttc attacttatc cagatgtaag cccacttaaa gcgatttaac aattatttgc
     6421 cgaaagagta taaacaaatt tcacataaaa atggattaag aaaagcttgt gtaagattat
     6481 gcgcagcgtt gccagatagc tccatttaaa acacttcaaa aacaataagt ttagaaaata
     6541 tatacataaa tagcagtcgt tgccgcaacg ctcaacacat cacactttta aaacaccctt
     6601 tacctacaca gaattacttt ttaaatttcc agtcaagctg cgagtttcaa aattatagcc
     6661 ggtagagaag acagtgctat ttcaaaagca aactaacaag ggtcttaaat ttcaaaacac
     6721 caatcctaac aagccttgga cttttgtaag tttagatcaa aggtggcatt gcattcaatg
     6781 tcatggtaag aagtaggtcg tctaggtaga aatcctcatt cagccggtca agtcagtacg
     6841 agaaaggtct caatttgaaa ttgtcttaaa aatattttat tgttttgtac tgtggtgagt
     6901 ttaaacgaaa aacacaaaaa aaaagtgata cacagaaatc ataaaaaatt ttaatacaag
     6961 gtattcgtac gtatcaaaaa catttcggca caattttttt tctctgtact aaagtgttac
     7021 gaacactacg gtatttttta gtgattttca acggacaccg aaggtatata aacagcgttc
     7081 gcgaacggtc gccttcaaaa ccaattgaca tttgcagcag caagtacaag tagaaagtaa
     7141 agcgcaatca gcgaaaaatt tatacttaat tgttggtgat taaagtacaa ttaaaagaac
     7201 attctcgaaa gtcacaagaa acgtaagttt ttaactcgct gttaccaatt agtaataaga
     7261 gcaacaagac gttgagtaat ttcaagaaaa actgcatttc aaggtctttg ttcggccatt
     7321 ttttttttat tcaacgctct acgtaattac aaaataagaa attggcagcc acgcatcttg
     7381 ttttcccaat gaattggcat caaaacgcaa acaaatctat aaataaaact tgcgtgttga
     7441 ttttcgccaa gatttattgg caaattgtga aattcgcagt gacgcatttg aaaattcgag
     7501 aaatcacgaa cgcactcgag catttgtgtg catgttatta gttagttgtt tagttaattg
     7561 aagtatttta ccaacgaaat ccacttattt ttagctgaaa tagagtaggt tgcttaaaca
     7621 aagccacgtc tgaaaatttc ttattgcttg tagttgtgac gtcaccatat acacacaaaa
     7681 taatgtgtat gcatgcgttt cagctgtgta tatatacatg cacacactcg cattatgaaa
     7741 acgatgacga gcaacggaac aggtttctca actacctttg ttcctgtttc ttcgctttcc
     7801 tttgttccaa tattcgtaga gggttaatag gggtttctca acaaagttgg cgtcgataaa
     7861 taagtttccc atttttattc cccagccagg aagttagttt caatagtttt gtaatttcaa
     7921 cgaaactcat ttgatttcgt actaattttc cacatctcta ttttctgccc gcagaataat
     7981 ccaactgaat agggaattgg gaacttaaaa aaaaaaatca aaatgagcgg taccgatcgc
     8041 agccccccca agaagaagcg caaggtggag ttcggatccg gtggcggcgg aagtggaggt
     8101 ggaggctcga tggtgtccaa gggcgaggag ctgatcaagg agaacatgca catgaagctg
     8161 tacatggagg gcaccgtgga caaccaccac ttcaagtgca cctccgaggg cgagggcaag
     8221 ccctacgagg gcacccagac catgcgcatc aaggtggtgg agggcggccc cctgcccttc
     8281 gccttcgaca tcctggccac ctccttcctg tacggctcca agaccttcat caaccacacc
     8341 cagggcatcc ccgacttctt caagcagtcc ttccccgagg gcttcacctg ggagcgcgtg
     8401 accacctacg aggacggcgg cgtgctgacc gccacccagg acacctccct gcaagacggc
     8461 tgcctgatct acaacgtgaa gatccgcggc gtgaacttca cctccaacgg ccccgtgatg
     8521 cagaagaaga ccctgggctg ggaggccttc accgagaccc tgtaccccgc cgacggcggc
     8581 ctggagggcc gcaacgacat ggccctgaag ctggtgggcg gctcccacct gatcgccaac
     8641 gccaagacca cctaccgctc caagaagccc gccaagaacc tgaagatgcc cggcgtgtac
     8701 tacgtggact accgcctgga gcgcatcaag gaggccaaca acgagaccta cgtggagcag
     8761 cacgaggtgg ccgtggcccg ctactgcgac ctgccctcca agctgggcca caagctgaac
     8821 taatctagaa ttaattaact aagccagtcg gcaatcggac gccttcgaaa catgcaacac
     8881 taatgtttaa ttcagatttc agcagagaca agctaaaaca ccgacgagtt gtaatcattt
     8941 ctgtgcgcca gcatatattt cttatataca acgtaataca taattatgta attctagcat
     9001 ctccccaaca ctcacataca aacaaacaaa aaatacaaac acacaaaacg tatttacccg
     9061 cacgcatcct tggcgaggtt gagtatgaaa caaaaacaaa acttaattta gagcaaagta
     9121 attacacgaa taaatttaat aaaaaaaaac tataataaaa acgccaatca tgttatttcc
     9181 aaaaaaaaaa aaaaaaaaac tagacgagat gtttgcgctt gtgtcgatga atgtaaagaa
     9241 ttgcaactac atagatggta attaatttca gctgtaagaa gtacaagttt ttcataattg
     9301 tgagaaaagt gcagtagtat cgcttaaagt tcagttttaa tcagggcatt tagcagctcg
     9361 aacccaatgt atttgtaagc ccaataaacg tgtaaatgcc tagagcaaaa tatcacaact
     9421 gtagcttttc atccgaaaga taataatgaa tagactgacc tgacgttttt caaaatgcga
     9481 ctagtggcct aattctagta tgtatgtaag ttaataaaac ccatttttgc ggaaagtaga
     9541 taaaaaaaac attttttttt tttactgcac tggatatcat tgaacttatc tgatcagttt
     9601 taaatttact tcgatccaag ggtatttgat gtaccaggtt ctttcgatta cctctcactc
     9661 aaaatgacat tccactcaaa gtcagcgctg tttgcctcct tctctgtcca cagaaatatc
     9721 gccgtctctt tcgccgctgc gtccgctatc tctttcgcca ccgtttgtag cgttacgtag
     9781 cgtcaatgtc cgccttcagt tgcattttgt cagcggtttc gtgacgaagc tccaagcggt
     9841 ttacgccatc aattaaacac aaagtgctgt gccaaaactc ctctcgcttc ttatttttgt
     9901 ttgttttttg agtgattggg gtggtgattg gttttgggtg ggtaagcagg ggaaagtgtg
     9961 aaaaatcccg gcaatgggcc aagaggatca ggagctatta attcgcggag gcagcaaaca
    10021 cccatctgcc gagcatctga acaatgtgag tagtacatgt gcatacatct taagttcact
    10081 tgatctatag gaactgcgat tgcaacatca aattgtctgc ggcgtgagaa ctgcgaccca
    10141 caaaaatccc aaaccgcaat tgcacaaaca aatagtgaca cgaaacagat tattctggta
    10201 gctgttctcg ctatataaga caatttttga gatcatatca tgatcaagac atctaaaggc
    10261 attcattttc gactatattc ttttttacaa aaaatataac aaccagatat tttaagctga
    10321 tcctagatgc acaaaaaata aataaaagta taaacctact tcgtaggata cttcggggta
    10381 ctttttgttc ggggttagat gagcataacg cttgtagttg atatttgaga tcccctatca
    10441 ttgcagggtg acagcggagc ggcttcgcag agctgcatta accagggctt cgggcaggcc
    10501 aaaaactacg gcacgctccg gccacccagt ccgccggagg actccggttc agggagcggc
    10561 caactagccg agaacctcac ctatgcctgg cacaatatgg acatctttgg ggcggtcaat
    10621 cagccgggct ccggatggcg gcagctggtc aaccggacac gcggactatt ctgcaacgag
    10681 cgacacatac cggcgcccag gaaacatttg ctcaagaacg gtgagtttct attcgcagtc
    10741 ggctgatctg tgtgaaatct taataaaggg tccaattacc aatttgaaac tcagtttgcg
    10801 gcgtggccta tccgggcgaa cttttggccg tgatgggcag ttccggtgcc ggaaagacga
    10861 ccctgctgaa tgcccttgcc tttcgatcgc cgcagggcat ccaagtatcg ccatccggga
    10921 tgcgactgct caatggccaa cctgtggacg ccaaggagat gcaggccagg tgcgcctatg
    10981 tccagcagga tgacctcttt atcggctccc taacggccag ggaacacctg attttccaag
    11041 ccatggtgcg gatgccacga catctgacct atcggcagcg agtggcccgc gtggatcagg
    11101 tgatccagga gctttcgctc agcaaatgtc agcacacgat catcggtgtg cccggcaggg
    11161 tgaaaggtct gtccggcgga gaaaggaagc gtctggcatt cgcctccgag gctctaaccg
    11221 atccgccgct tctgatctgc gatgagccca cctccggact ggactccttt accgcccaca
    11281 gcgtcgtcca ggtgctgaag aagctgtcgc agaagggcaa gaccgtcatc ctgaccattc
    11341 atcagccgtc ttccgagctg tttgagctct ttgacaagat ccttctgatg gccgagggca
    11401 gggtagcttt cttgggcact cccagcgaag ccgtcgactt cttttcctag tgagttcgat
    11461 gtgtttatta agggtatcta gtattacata acatctcaac tcctatccag cgtgggtgcc
    11521 cagtgtccta ccaactacaa tccggcggac ttttacgtac aggtgttggc cgttgtgccc
    11581 ggacgggaga tcgagtcccg tgatcggatc gccaagatat gcgacaattt tgccattagc
    11641 aaagtagccc gggatatgga gcagttgttg gccaccaaaa atctggagaa gccactggag
    11701 cagccggaga atgggtacac ctacaaggcc acctggttca tgcagttccg ggcggtcctg
    11761 tggcgatcct ggctgtcggt gctcaaggaa ccactcctcg taaaagtgcg acttattcag
    11821 acaacggtga gtggttccag tggaaacaaa tgatataacg cttacaattc ttggaaacaa
    11881 attcgctaga ttttagatag aattgcctga ttccacaccc ttcttagttt ttttcaatga
    11941 gatgtatagt ttatagtttt gcagaagata aataaatttc atttaactcg cgaatattaa
    12001 tgagatgcga gtaacatttt aatttgcaga tggttgccat cttgattggc ctcatctttt
    12061 tgggccaaca actcacgcaa gtgggtgtga tgaatatcaa cggagccatc ttcctcttcc
    12121 tgaccaacat gacctttcaa aacgtctttg ccacgataaa tgtaagtcat gtttagaata
    12181 catttgcatt tcaataattt actaactttc taatgaatcg attcgattta ggtgttcacc
    12241 tcagagctgc cagtttttat gagggaggcc cgaagtcgac tttatcgctg tgacacatac
    12301 tttctgggca aaacgattgc cgaattgccg ctttttctca cagtgccact ggtcttcacg
    12361 gcgattgcct atccgatgat cggactgcgg gccggagtgc tgcacttctt caactgcctg
    12421 gcgctggtca ctctggtggc caatgtgtca acgtccttcg gatatctaat atcctgcgcc
    12481 agctcctcga cctcgatggc gctgtctgtg ggtccgccgg ttatcatacc attcctgctc
    12541 tttggcggct tcttcttgaa ctcgggctcg gtgccagtat acctcaaatg gttgtcgtac
    12601 ctctcatggt tccgttacgc caacgagggt ctgctgatta accaatgggc ggacgtggag
    12661 ccgggcgaaa ttagctgcac atcgtcgaac accacgtgcc ccagttcggg caaggtcatc
    12721 ctggagacgc ttaacttctc cgccgccgat ctgccgctgg actacgtggg tctggccatt
    12781 ctcatcgtga gcttccgggt gctcgcatat ctggctctaa gacttcgggc ccgacgcaag
    12841 gagtagccga catatatccg aaataactgc ttgttttttt tttttaccat tattaccatc
    12901 gtgtttactg tttattgccc cctcaaaaag ctaatgtaat tatatttgtg ccaataaaaa
    12961 caagatatga cctatagaat acaagtattt ccccttcgaa catccccaca agtagacttt
    13021 ggatttgtct tctaaccaaa agacttacac acctgcatac cttacatcaa aaactcgttt
    13081 atcgctacat aaaacaccgg gatatatttt ttatatacat acttttcaaa tcgcgcgccc
    13141 tcttcataat tcacctccac cacaccacgt ttcgtagttg ctctttcgct gtctcccacc
    13201 cgctctccgc aacacattca ccttttgttc gacgaccttg gagcgactgt cgttagttcc
    13261 gcgcgattcg gttcgctcaa atggttccga gtggttcatt tcgtctcaat agaaattagt
    13321 aataaatatt tgtatgtaca atttatttgc tccaatatat ttgtatatat ttccctcaca
    13381 gctatattta ttctaattta atattatgac tttttaaggt aattttttgt gacctgttcg
    13441 gagtgattag cgttacaatt tgaactgaaa gtgacatcca gtgtttgttc cttgtgtaga
    13501 tgcatctcaa aaaaatggtg ggcataatag tgttgtttat atatatcaaa aataacaact
    13561 ataataataa gaatacattt aatttagaaa atgcttggat ttcactggaa ctagaattaa
    13621 ttcggctgct gctctaaacg acgcatttcg tactccaaag tacgaatttt ttccctcaag
    13681 ctcttatttt cattaaacaa tgaacaggac ctaacgcaca gtcacgttat tgtttacata
    13741 aatgattttt tttactattc aaacttactc tgtttgtgta ctcccactgg tatagccttc
    13801 ttttatcttt tctggttcag gctctatcac tttactaggt acggcatctg cgttgagtcg
    13861 cctcctttta aatgtctgac cttttgcagg tgcagccttc cactgcgaat cattaaagtg
    13921 ggtatcacaa atttgggagt tttcaccaag gctgcaccca aggctctgct cccacaattt
    13981 tctcttaata gcacacttcg gcacgtgaat taattttact ccagtcacag ctttgcagca
    14041 aaatttgcaa tatttcattt ttttttattc cacgtaaggg ttaatgtttt caaaaaaaaa
    14101 ttcgtccgca cacaaccttt cctctcaaca agcaaacgtg cactgaattt aagtgtatac
    14161 ttcggtaagc ttcggctatc gacgggacca ccttatgtta tttcatcatg ggccagaccc
    14221 acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat gaccttgccc
    14281 gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc cacgtccgcc
    14341 cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta cgacaaccat
    14401 ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca taggctccgc
    14461 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
    14521 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
    14581 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
    14641 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
    14701 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
    14761 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
    14821 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    14881 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    14941 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    15001 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    15061 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    15121 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    15181 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    15241 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    15301 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    15361 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    15421 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    15481 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    15541 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    15601 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    15661 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    15721 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    15781 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    15841 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    15901 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    15961 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    16021 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    16081 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    16141 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    16201 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
    16261 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
    16321 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
    16381 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
    16441 gtgcaccata tcgacatgcc cgccgtgacc gtcgagaacc cgctgacgct gccccgcgta
    16501 tccgcacccg ccgacgccgt cgcacgtccc gtgctcaccg tgaccaccgc gcccagcggt
    16561 ttcgagggcg agggcttccc ggtgcgccgc gcgttcgccg ggatcaacta ccgccacctc
    16621 gacccgttca tcatgatgga ccagatgggt gaggtggagt acgcgcccgg ggagcccaag
    16681 ggcacgccct ggcacccgca ccgcggcttc gagaccgtga cctacatcgt cgatatgcgg
    16741 tgtgaaatac cgcaccgaat cgcgcggaac taacgacagt cgctccaagg tcgtcgaaca
    16801 aaaggtgaat gtgttgcgga gagcgggtgg gagacagcga aagagcaact acgaaacgtg
    16861 gtgtggtgga ggtgaattat gaagagggcg cgcgatttga aaagtatgta tataaaaaat
    16921 atatcccggt gttttatgta gcgataaacg agtttttgat gtaaggtatg caggtgtgta
    16981 agtcttttgg ttagaagaca aatccaaagt ctacttgtgg ggatgttcga aggggaaata
    17041 cttgtattct ataggtcata tcttgttttt attggcacaa atataattac attagctttt
    17101 tgagggggca ataaacagta aacacgatgg taataatggt aaaaaaaaaa aacaagcagt
    17161 tatttcggat atatgtcggc tactccttgc gtcgggcccg aagtcttaga gccagatatg
    17221 cgagcacccg gaagctcacg atgagaatgg ccagac
//
