LOCUS       sequence-381834-        4664 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synER Cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Sanford A, Kiriakov S, Khalil AS
  TITLE     A Toolkit for Precise, Multigene Control in Saccharomyces 
            cerevisiae.
  JOURNAL   ACS Synth Biol. 2022 Nov 11. doi: 10.1021/acssynbio.2c00423.
  PUBMED    36367334
REFERENCE   2  (bases 1 to 4664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2022 Nov 11. doi: 10.1021/acssynbio.2c00423."
COMMENT     Sequence Label: pAS161
FEATURES             Location/Qualifiers
     source          1..4664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        473..886
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     terminator      2398..2585
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2453..2477)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(join(2902..3693,3694..3762))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3525..3544
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3763..3867)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3970..4558
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4459..4478
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cccctgaatt cgcatctaga acggggtcat cacggctcat catgcgccaa acaaatgtgt
       61 gcaatacacg ctcggatgac tgcatgatga ccgcactgac tggggacagc agatccacct
      121 aagcctgtga gagaagcaga cacccgacag atcaaggcag ttacgtctca ccaaaccaga
      181 tgtcaacaca gctacaacgc cttgccaaca gggagttctt cagagacatg gaggctcaaa
      241 acgaaattat tgacagccta gacatcaata gtcatacaac agaaagcgac cacccaactt
      301 tggctgataa tagcgtataa acaatgcata ctttgtacgt tcaaaataca atgcagtaga
      361 tatatttatg catattacat ataatacata tcacatagga agcaacaggc gcgttggact
      421 tttaattttc gaggaccgcg aatccttaca tcacacccaa tcccccacaa gtgatccccc
      481 acacaccata gcttcaaaat gtttctactc cttttttact cttccagatt ttctcggact
      541 ccgcgcatcg ccgtaccact tcaaaacacc caagcacagc atactaaatt tcccctcttt
      601 cttcctctag ggtgtcgtta attacccgta ctaaaggttt ggaaaagaaa aaagacaccg
      661 cctcgtttct ttttcttcgt cgaaaaaggc aataaaaatt tttatcacgt ttctttttct
      721 tgaaaatttt tttttttgat ttttttctct ttcgatgacc tcccattgat atttaagtta
      781 ataaacggtc atcaatttct caagtttcag tttcattttt cttgttctat tacaactttt
      841 tttacttctt gctcattaga aagaaagcat agcaatctaa tctaagtttt aattacaaaa
      901 gatctatgct cgagaaaaaa atggagcgtc ccttccaatg tagaatttgc atgagaaact
      961 tttcgagaca atctaatttg tcaagacata cccgtactca taccggtgaa aaaccgtttc
     1021 agtgtcggat atgtatgcga aatttctcaa gaaatgaaca tttggttttg catctacgta
     1081 cgcacaccgg cgagaagcca ttccaatgca gaatatgtat gagaaacttt agtcaaaaaa
     1141 ctggtttgag agttcaccta aaaacacatc tgagaggtga tggtgctgga ttgattgata
     1201 tcgattctgc tggtgacatg agagctgcaa acctttggcc aagcccgtta atgattaaac
     1261 gatctaagaa aaacagttta gctttgtccc tgacggctga tcaaatggta agtgccttgt
     1321 tggatgctga acctccaata ctctattcag aatatgatcc tacaagacct ttcagtgaag
     1381 cttcgatgat gggcttactt actaacttag cagacaggga gttagttcac atgatcaact
     1441 gggcaaaaag ggtaccaggt tttgttgatt tgaccttaca tgatcaagtc caccttctag
     1501 aatgtgcctg gctagaaatc ctaatgattg gacttgtctg gcgctcaatg gaacacccag
     1561 tgaagctact ttttgctcct aatttgttat tggacaggaa tcaaggaaaa tgtgtagagg
     1621 gcatggttga gattttcgac atgttgttag ctacatcatc tagattccgc atgatgaatc
     1681 tgcaaggtga agagtttgtg tgcctcaaat ctattatttt gcttaattct ggtgtgtaca
     1741 catttttgtc cagcactctg aagtctctgg aagaaaaaga tcatattcat agagtcctgg
     1801 acaagatcac agatactttg atccacttaa tggcaaaagc aggcttaacc cttcagcagc
     1861 agcaccagag gttagctcaa ttattactca tcttatccca cattaggcat atgagtaaca
     1921 aagggatgga acatctttat agtatgaagt gcaagaatgt cgtgccactc tatgatctgt
     1981 tgttagaaat gttagacgcc cataggctac atgcgccaac ttctcgtgga ggggcatccg
     2041 ttgaagaaac agaccaaagc catttggcca ctgcaggttc tacttcatcg cattccttgc
     2101 aaaagtatta cataacgggt gaagcagaag gtttccctgc cacagttggt gatggagcag
     2161 gcttaataaa tgctagccat ttagatggcg aggacgtagc gatggcgcat gctgatgcgc
     2221 tagatgattt tgatttagac atgttggggg atggtgattc acctggtcca ggatttactc
     2281 cccacgactc cgccccctac ggtgctctag atatggccga cttcgaattt gaacaaatgt
     2341 ttaccgatgc acttggaatt gacgagtacg gtgggtaagg gcccatccta actcgaggcg
     2401 aatttcttat gatttatgat ttttattatt aaataagtta taaaaaaaat aagtgtatac
     2461 aaattttaaa gtgactctta ggttttaaaa cgaaaattct tattcttgag taactctttc
     2521 ctgtaggtca ggttgctttc tcaggtatag catgaggtcg ctcttattga ccacacctct
     2581 accggcatgc cgagcaaatg cctgcaaatc gctccccatt tcgctgagca tgagacggaa
     2641 atctgctcgt cagtggtgct cacactgacg aatcatgtac agatcatacc gatgactgcc
     2701 tggcgactca caactaagca agacagccgg aaccagcgcc ggcgaacacc actgcatata
     2761 tggcatatca caacagtcca actagtgcac tgcagtacag cggccgcgat tatcaaaaag
     2821 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     2881 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     2941 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     3001 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgggacccac gctcaccggc
     3061 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     3121 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     3181 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     3241 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     3301 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     3361 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     3421 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     3481 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     3541 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     3601 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     3661 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     3721 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     3781 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     3841 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgtcatgac
     3901 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3961 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     4021 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     4081 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     4141 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     4201 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     4261 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     4321 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     4381 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4441 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4501 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4561 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     4621 ctttcctgcg ttatcccctg attctgtgga taaccgtgcg gccg
//
