LOCUS       sequence-381835-       17205 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To insert T2A-LexAGAD into Gal4 coding sequence in Drosophila, 
            converting Gal4 into a T2A-LexAGAD transgene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17205)
  AUTHORS   Karuparti S, Yeung AT, Wang B, Guicardi PF, Han C
  TITLE     A toolkit for converting Gal4 into LexA and Flippase transgenes in 
            Drosophila.
  JOURNAL   G3 (Bethesda). 2023 Mar 9;13(3):jkad003. doi: 
            10.1093/g3journal/jkad003.
  PUBMED    36617215
REFERENCE   2  (bases 1 to 17205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-03-9"; volume: "13(3):jkad003. doi"; 
            pages: " 10.1093/g3journal/jkad003"
COMMENT     Sequence Label: pHACK(Gal4)-DONR(T2A-LexAGAD)
FEATURES             Location/Qualifiers
     source          1..17205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(1..31,32..233)
                     /label=P element 3' end
                     /note="P element 3' end"
     CDS             246..686
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             1368..1421
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1446..1466
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1476..1727,1728..2081)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     promoter        5460..5880
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     CDS             7995..8015
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(9453..9471)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(9923..9994,10396..10669,10744..11398,11460..11775,
                     11979..12110,12181..12795)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(13574..14128,14129..14159)
                     /label=P element 5' end
     rep_origin      complement(14394..14982)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14474..14493)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15153..15944,15945..16013))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15776..15795
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16014..16118)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     16186..16204
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(16242..16264)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     16364..16383
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    16593..16662
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
       61 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
      121 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
      181 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
      241 gctgcatgaa gctactgtct tctatcgaac aagcatgcga tatttgccga cttaaaaagc
      301 tcaagtgctc caaagaaaaa ccgaagtgcg ccaagtgtct gaagaacaac tgggagtgtc
      361 gctactctcc caaaaccaaa aggtctccgc tgactagggc acatctgaca gaagtggaat
      421 caaggctaga aagactggaa cagctatttc tactgatttt tcctcgagaa gaccttgaca
      481 tgattttgaa aatggattct ttacaggata taaaagcatt gttaacagga ttatttgtac
      541 aagataatgt gaataaagat gccgtcacag atagattggc ttcagtggag actgatatgc
      601 ctctaacatt gagacagcat agaataagtg cgacatcatc atcggaagag agtagtaaca
      661 aaggtcaaag acagttgact gtatcgattg actcggcagc tcatcatgat aactccacaa
      721 ttccgttgga ttttatgccc agggatgctc ttcatggatt tgattggtct gaagaggatg
      781 acatgtcgga tggcttgccc ttcctgaaaa cggaccccaa caataatggg ttctttggcg
      841 acggttctct cttatgtatt cttcgatcta ttggctttaa accggaaaat tacacgaact
      901 ctaacgttaa caggctcccg accatgatta cggatagata cacgttggct tctagatcca
      961 caacatcccg tttacttcaa agttatctca ataattttca cccctactgc cctatcgtgc
     1021 actcaccgac gctaatgatg ttgtataata accagattga aatcgcgtcg aaggatcaat
     1081 ggcaaatcct ttttaactgc atattagcca ttggagcctg gtgtatagag ggggaatcta
     1141 ctgatataga tgttttttac tatcaaaatg ctaaatctca tttgacgagc aaggtcttcg
     1201 agtcaggttc cataattttg gtgacagccc tacatcttct gtcgcgatat acacagtgga
     1261 ggcagaaaac aaatactagc tataattttc acagcttttc cataagaatg gccatatcat
     1321 tgggcttgaa tagggacctc ccctcgtcct tcagtgatag cagcggagag ggcagaggaa
     1381 gtctgctaac atgcggtgac gtcgaggaga atcctggccc aggaagcgga ggcgctagca
     1441 tgccacccaa gaagaagcga aaagtagaag atccaatgaa ggctctcacg gcccgacaac
     1501 aggaagtttt tgatttgata cgggatcata tatcccaaac gggtatgcct ccgacccgcg
     1561 cagagatagc acagcgactg ggctttcgat cgcctaacgc cgcggaggag cacttgaagg
     1621 cactggcccg caagggtgtc attgaaatcg tgtccggtgc gagccgcgga atccggctgt
     1681 tgcaggagga ggaagagggc ctgccactgg tgggacgcgt ggccgctggc gagccgctgc
     1741 tggcccagca acacatagag ggacactatc aggtggaccc ctccttgttt aagccaaatg
     1801 ctgatttcct gttgcgggtg tcgggaatgt ccatgaagga catcggtatt atggatggtg
     1861 acctcctcgc cgtccataag acacaggatg tcaggaacgg ccaggtagtc gttgccagga
     1921 tagacgatga ggtcactgtg aaacgtctca agaagcaagg caataaggtc gagctgctgc
     1981 cggagaatag cgagttcaag ccgatcgtgg tggatctgcg acagcagtcc tttactatcg
     2041 agggcttggc cgtgggtgtg atccgcaacg gagattggct gggatccatc gacagtgcgg
     2101 cccaccacga caacagcacc attccactgg acttcatgcc tcgtgacgca ctgcatggat
     2161 ttgactggag cgaggaggat gatatgagcg acggactccc attcctgaag accgacccca
     2221 ataacaatgg tttctttggc gacggcagcc tgctgtgtat cctgcgaagt attggtttta
     2281 agccggaaaa ttacacgaac agtaatgtca atcgtcttcc cacaatgatc accgaccgtt
     2341 acacgctggc cagtcgcagt actacaagcc gcctgctgca aagctatctt aacaacttcc
     2401 acccctactg ccccatagtg catagtccta ctctcatgat gctttacaac aatcagattg
     2461 agatagcatc gaaggaccag tggcagatcc tcttcaactg cattctcgcc atcggcgctt
     2521 ggtgtattga aggcgagagc acagatatcg acgtgtttta ctatcaaaac gccaagtcgc
     2581 acctcacctc gaaggtcttc gaaagtggaa gcattatcct cgtgacagcc ctccatctgc
     2641 tgagtcgtta tacccagtgg cgtcagaaaa ctaatacatc ctacaacttt cattcgttca
     2701 gcattcggat ggcgattagc ctgggactta atcgtgacct cccaagtagc ttcagcgatt
     2761 cgtcgatcct ggaacagcgc cggaggattt ggtggagtgt gtacagctgg gagatccagc
     2821 tgtccctctt gtacggacgc agcattcagc tgagtcagaa cacgatttcc ttcccgtcca
     2881 gcgtggacga cgttcaacgg acaactacgg gcccgaccat ctaccacggt atcattgaga
     2941 cggcccgctt gcttcaagtg tttacgaaga tttatgagct ggataagaca gtgactgcag
     3001 aaaaaagtcc tatatgtgca aaaaaatgct tgatgatttg taatgagatt gaggaggttt
     3061 cgagacaggc accaaagttt ttacaaatgg atatttccac caccgctcta accaatttgt
     3121 tgaaggaaca cccttggcta tcctttacaa gattcgaact gaagtggaaa cagttgtctc
     3181 ttatcattta tgtattaaga gattttttca ctaattttac ccagaaaaag tcacaactag
     3241 aacaggatca aaatgatcat caaagttatg aagttaaacg atgctccatc atgttaagcg
     3301 atgcagcaca aagaactgtt atgtctgtaa gtagctatat ggacaatcat aatgtcaccc
     3361 catattttgc ctggaattgt tcttattact tgttcaatgc agtcctagta cccataaaga
     3421 ctctactctc aaactcaaaa tcgaatgctg agaataacga gaccgcacaa ttattacaac
     3481 aaattaacac tgttctgatg ctattaaaaa aactggccac ttttaaaatc cagacttgtg
     3541 aaaaatacat tcaagtactg gaagaggtat gtgcgccgtt tctgttatca cagtgtgcaa
     3601 tcccattacc gcatatcagt tataacaata gtaatggtag cgccattaaa aatattgtcg
     3661 gttctgcaac tatcgcccaa taccctactc ttccggagga aaatgtcaac aatatcagtg
     3721 ttaaatatgt ttctcctggc tcagtagggc cttcacctgt gccattgaaa tcaggagcaa
     3781 gtttcagtga tctagtcaag ctgttatcta accgtccacc ctctcgtaac tctccagtga
     3841 caataccaag aagcacacct tcgcatcgct cagtcacgcc ttttctaggg caacagcaac
     3901 agctgcaatc attagtgcca ctgaccccgt ctgctttgtt tggtggcgcc aattttaatc
     3961 aaagtgggaa tattgctgat agctcattgt ccttcacttt cactaacagt agcaacggtc
     4021 cgaacctcat aacaactcaa acaaattctc aagcgctttc acaaccaatt gcctcctcta
     4081 acgttcatga taacttcatg aataatgaaa tcacggctag taaaattgat gatggtaata
     4141 attcaaaacc actgtcacct ggttggacgg accaaactgc gtataacaaa gtgaacacgt
     4201 cgctaagcga aagcccgttt tgtcatcgct ttttgtcggg tctcagttgt ggatcgaaaa
     4261 cccggagagt aaccccttga ccccttgtca gtttccgcat gctccagccc ttgtcctcat
     4321 attttccaat cgtttttggc gtttttagct gcggccattg aatgttcaaa aattattgta
     4381 aacttttcag accccctccg tgtgtgtgtg tgtgtgtgtg cttctgtgta tgtggaaaag
     4441 ttcgaattct agttgggaaa tcaattaaaa gcgcttgttg gactcgcacc acaactttcc
     4501 ctttcgatcg cccctgcggc ttggttcaac tggtcaaggc ctctagctcc cgaaaaccgt
     4561 cgaaaattgg tttggcactt tttatagcca cccaatataa taccaagttc agacagaaat
     4621 tgatatgggt attggactga tcaataactt aagttccctc actgaattat ccttaactct
     4681 tgtgcatgcc tatttatgtg aaaacaatca aaattactca attatgtatg cacaggtatt
     4741 gaaagctccc actatttagt ttttgtttta attatctgtc tttagaactt taaatggaaa
     4801 aacatgactt atatgtatga acaaaaattc caatcgatca ctttaggcgg ccgccaatgc
     4861 caacagaagc attatttttt tcctttcccg atctctcaag cacactcagt tgatctcagt
     4921 cgcaaaactc gttactcact cttaatattt cctcttcttt gcactttcct ggtggaatgc
     4981 attctctatt ttcctctccc tactgcacat actctttgag ctctctagca gataccctaa
     5041 caatttctaa tcatccacga ataataaaca aagatttcta atatcttaaa cctgttttat
     5101 atacttatta ttcaaaaaaa aaagtgtggc aaaaaaaaag agcacttcaa cgtttgttga
     5161 acaaaggata taattttgta agggtatcta gaagttttca tgcctctctt catccactct
     5221 tctcaacctc atgcgatagc tgctgcgctc tctcgtttat cgttcggttg aggttttata
     5281 attctcaaat actttttccc gaagtggtgc ctttaaatag cgtatatgag tggaagactt
     5341 tcgatgtgca gcgtaccaca acgtttaaga gctatgctgg aaacagcata gcaagtttaa
     5401 ataaggctag tccgttatca actttgctgg aaacagcaaa gtggcaccga gtcggtgctt
     5461 tttttgctca cctgtgattg ctcctactca aatacaaaaa catcaaattt tctgtcaata
     5521 aagcatattt atttatattt attttacagg aaagaattcc ttttaaagtg tattttaacc
     5581 tataatgaaa aacgattaaa aaaaatacat aaaataattc gaaaattttt gaatagccca
     5641 ggttgataaa aattcatttc atacgtttta taacttatgc ccctaagtat tttttgacca
     5701 tagtgtttca attctacatt aattttacag agtagaatga aacgccacct actcagccaa
     5761 gaggcgaaaa ggttagctcg ccaagcagag agggcgccag tgctcactac tttttataat
     5821 tctcaacttc tttttccaga ctcagttcgt atatatagac ctattttcaa tttaacgtcg
     5881 tgtattctga gaaagctgga gtttaagagc tatgctggaa acagcatagc aagtttaaat
     5941 aaggctagtc cgttatcaac tttgctggaa acagcaaagt ggcaccgagt cggtgctttt
     6001 tttgcctacc tggagcctga gagttgttca ataaaataaa aatgtttcgt ttttttgctt
     6061 tcgccagtat ttattatttt tcatcaatat gtattcaatt tggtatgtat ttacgcgtgg
     6121 cttgctgttc ttcgcgttca aaatctccag ctccattttg ctttcggtgc gcttgcaatc
     6181 agtactgtcc aaaatcgaaa atcgccgaac cgtagtgtga ccgtgcgggg ctctgcgaaa
     6241 ataaactttt ttaggtatat ggccacacac ggggaaagca cagtggatta tatgtattaa
     6301 tatattatgc aggttttcat tacttatcca gatgtaagcc cacttaaagc gatttaacaa
     6361 ttatttgccg aaagagtata aacaaatttc acataaaaat ggattaagaa aagcttgtgt
     6421 aagattatgc gcagcgttgc cagatagctc catttaaaac acttcaaaaa caataagttt
     6481 agaaaatata tacataaata gcagtcgttg ccgcaacgct caacacatca cacttttaaa
     6541 acacccttta cctacacaga attacttttt aaatttccag tcaagctgcg agtttcaaaa
     6601 ttatagccgg tagagaagac agtgctattt caaaagcaaa ctaacaaggg tcttaaattt
     6661 caaaacacca atcctaacaa gccttggact tttgtaagtt tagatcaaag gtggcattgc
     6721 attcaatgtc atggtaagaa gtaggtcgtc taggtagaaa tcctcattca gccggtcaag
     6781 tcagtacgag aaaggtctca atttgaaatt gtcttaaaaa tattttattg ttttgtactg
     6841 tggtgagttt aaacgaaaaa cacaaaaaaa aagtgataca cagaaatcat aaaaaatttt
     6901 aatacaaggt attcgtacgt atcaaaaaca tttcggcaca attttttttc tctgtactaa
     6961 agtgttacga acactacggt attttttagt gattttcaac ggacaccgaa ggtatataaa
     7021 cagcgttcgc gaacggtcgc cttcaaaacc aattgacatt tgcagcagca agtacaagta
     7081 gaaagtaaag cgcaatcagc gaaaaattta tacttaattg ttggtgatta aagtacaatt
     7141 aaaagaacat tctcgaaagt cacaagaaac gtaagttttt aactcgctgt taccaattag
     7201 taataagagc aacaagacgt tgagtaattt caagaaaaac tgcatttcaa ggtctttgtt
     7261 cggccatttt ttttttattc aacgctctac gtaattacaa aataagaaat tggcagccac
     7321 gcatcttgtt ttcccaatga attggcatca aaacgcaaac aaatctataa ataaaacttg
     7381 cgtgttgatt ttcgccaaga tttattggca aattgtgaaa ttcgcagtga cgcatttgaa
     7441 aattcgagaa atcacgaacg cactcgagca tttgtgtgca tgttattagt tagttgttta
     7501 gttaattgaa gtattttacc aacgaaatcc acttattttt agctgaaata gagtaggttg
     7561 cttaaacaaa gccacgtctg aaaatttctt attgcttgta gttgtgacgt caccatatac
     7621 acacaaaata atgtgtatgc atgcgtttca gctgtgtata tatacatgca cacactcgca
     7681 ttatgaaaac gatgacgagc aacggaacag gtttctcaac tacctttgtt cctgtttctt
     7741 cgctttcctt tgttccaata ttcgtagagg gttaataggg gtttctcaac aaagttggcg
     7801 tcgataaata agtttcccat ttttattccc cagccaggaa gttagtttca atagttttgt
     7861 aatttcaacg aaactcattt gatttcgtac taattttcca catctctatt ttctgcccgc
     7921 agaataatcc aactgaatag ggaattggga acttaaaaaa aaaaatcaaa atgagcggta
     7981 ccgatcgcag cccccccaag aagaagcgca aggtggagtt cggatccggt ggcggcggaa
     8041 gtggaggtgg aggctcgatg gtgtccaagg gcgaggagct gatcaaggag aacatgcaca
     8101 tgaagctgta catggagggc accgtggaca accaccactt caagtgcacc tccgagggcg
     8161 agggcaagcc ctacgagggc acccagacca tgcgcatcaa ggtggtggag ggcggccccc
     8221 tgcccttcgc cttcgacatc ctggccacct ccttcctgta cggctccaag accttcatca
     8281 accacaccca gggcatcccc gacttcttca agcagtcctt ccccgagggc ttcacctggg
     8341 agcgcgtgac cacctacgag gacggcggcg tgctgaccgc cacccaggac acctccctgc
     8401 aagacggctg cctgatctac aacgtgaaga tccgcggcgt gaacttcacc tccaacggcc
     8461 ccgtgatgca gaagaagacc ctgggctggg aggccttcac cgagaccctg taccccgccg
     8521 acggcggcct ggagggccgc aacgacatgg ccctgaagct ggtgggcggc tcccacctga
     8581 tcgccaacgc caagaccacc taccgctcca agaagcccgc caagaacctg aagatgcccg
     8641 gcgtgtacta cgtggactac cgcctggagc gcatcaagga ggccaacaac gagacctacg
     8701 tggagcagca cgaggtggcc gtggcccgct actgcgacct gccctccaag ctgggccaca
     8761 agctgaacta atctagaatt aattaactaa gccagtcggc aatcggacgc cttcgaaaca
     8821 tgcaacacta atgtttaatt cagatttcag cagagacaag ctaaaacacc gacgagttgt
     8881 aatcatttct gtgcgccagc atatatttct tatatacaac gtaatacata attatgtaat
     8941 tctagcatct ccccaacact cacatacaaa caaacaaaaa atacaaacac acaaaacgta
     9001 tttacccgca cgcatccttg gcgaggttga gtatgaaaca aaaacaaaac ttaatttaga
     9061 gcaaagtaat tacacgaata aatttaataa aaaaaaacta taataaaaac gccaatcatg
     9121 ttatttccna aaaaaaaaaa aaaaaaaact agacgagatg tttgcgcttg tgtcgatgaa
     9181 tgtaaagaat tgcaactaca tagatggtaa ttaatttcag ctgtaagaag tacaagtttt
     9241 tcataattgt gagaaaagtg cagtagtatc gcttaaagtt cagttttaat cagggcattt
     9301 agcagctcga acccaatgta tttgtaagcc caataaacgt gtaaatgcct agagcaaaat
     9361 atcacaactg tagcttttca tccgaaagat aataatgaat agactgacct gacgtttttc
     9421 aaaatgcgac tagtggccta attctagtat gtatgtaagt taataaaacc catttttgcg
     9481 gaaagtagat aaaaaaaaca tttttttttt ttactgcact ggatatcatt gaacttatct
     9541 gatcagtttt aaatttactt cgatccaagg gtatttgatg taccaggttc tttcgattac
     9601 ctctcactca aaatgacatt ccactcaaag tcagcgctgt ttgcctcctt ctctgtccac
     9661 agaaatatcg ccgtctcttt cgccgctgcg tccgctatct ctttcgccac cgtttgtagc
     9721 gttacgtagc gtcaatgtcc gccttcagtt gcattttgtc agcggtttcg tgacgaagct
     9781 ccaagcggtt tacgccatca attaaacaca aagtgctgtg ccaaaactcc tctcgcttct
     9841 tatttttgtt tgttttttga gtgattgggg tggtgattgg ttttgggtgg gtaagcaggg
     9901 gaaagtgtga aaaatcccgg caatgggcca agaggatcag gagctattaa ttcgcggagg
     9961 cagcaaacac ccatctgccg agcatctgaa caatgtgagt agtacatgtg catacatctt
    10021 aagttcactt gatctatagg aactgcgatt gcaacatcaa attgtctgcg gcgtgagaac
    10081 tgcgacccac aaaaatccca aaccgcaatt gcacaaacaa atagtgacac gaaacagatt
    10141 attctggtag ctgttctcgc tatataagac aatttttgag atcatatcat gatcaagaca
    10201 tctaaaggca ttcattttcg actatattct tttttacaaa aaatataaca accagatatt
    10261 ttaagctgat cctagatgca caaaaaataa ataaaagtat aaacctactt cgtaggatac
    10321 ttcggggtac tttttgttcg gggttagatg agcataacgc ttgtagttga tatttgagat
    10381 cccctatcat tgcagggtga cagcggagcg gcttcgcaga gctgcattaa ccagggcttc
    10441 gggcaggcca aaaactacgg cacgctccgg ccacccagtc cgccggagga ctccggttca
    10501 gggagcggcc aactagccga gaacctcacc tatgcctggc acaatatgga catctttggg
    10561 gcggtcaatc agccgggctc cggatggcgg cagctggtca accggacacg cggactattc
    10621 tgcaacgagc gacacatacc ggcgcccagg aaacatttgc tcaagaacgg tgagtttcta
    10681 ttcgcagtcg gctgatctgt gtgaaatctt aataaagggt ccaattacca atttgaaact
    10741 cagtttgcgg cgtggcctat ccgggcgaac ttttggccgt gatgggcagt tccggtgccg
    10801 gaaagacgac cctgctgaat gcccttgcct ttcgatcgcc gcagggcatc caagtatcgc
    10861 catccgggat gcgactgctc aatggccaac ctgtggacgc caaggagatg caggccaggt
    10921 gcgcctatgt ccagcaggat gacctcttta tcggctccct aacggccagg gaacacctga
    10981 ttttccaagc catggtgcgg atgccacgac atctgaccta tcggcagcga gtggcccgcg
    11041 tggatcaggt gatccaggag ctttcgctca gcaaatgtca gcacacgatc atcggtgtgc
    11101 ccggcagggt gaaaggtctg tccggcggag aaaggaagcg tctggcattc gcctccgagg
    11161 ctctaaccga tccgccgctt ctgatctgcg atgagcccac ctccggactg gactccttta
    11221 ccgcccacag cgtcgtccag gtgctgaaga agctgtcgca gaagggcaag accgtcatcc
    11281 tgaccattca tcagccgtct tccgagctgt ttgagctctt tgacaagatc cttctgatgg
    11341 ccgagggcag ggtagctttc ttgggcactc ccagcgaagc cgtcgacttc ttttcctagt
    11401 gagttcgatg tgtttattaa gggtatctag tattacataa catctcaact cctatccagc
    11461 gtgggtgccc agtgtcctac caactacaat ccggcggact tttacgtaca ggtgttggcc
    11521 gttgtgcccg gacgggagat cgagtcccgt gatcggatcg ccaagatatg cgacaatttt
    11581 gccattagca aagtagcccg ggatatggag cagttgttgg ccaccaaaaa tctggagaag
    11641 ccactggagc agccggagaa tgggtacacc tacaaggcca cctggttcat gcagttccgg
    11701 gcggtcctgt ggcgatcctg gctgtcggtg ctcaaggaac cactcctcgt aaaagtgcga
    11761 cttattcaga caacggtgag tggttccagt ggaaacaaat gatataacgc ttacaattct
    11821 tggaaacaaa ttcgctagat tttagataga attgcctgat tccacaccct tcttagtttt
    11881 tttcaatgag atgtatagtt tatagttttg cagaagataa ataaatttca tttaactcgc
    11941 gaatattaat gagatgcgag taacatttta atttgcagat ggttgccatc ttgattggcc
    12001 tcatcttttt gggccaacaa ctcacgcaag tgggtgtgat gaatatcaac ggagccatct
    12061 tcctcttcct gaccaacatg acctttcaaa acgtctttgc cacgataaat gtaagtcatg
    12121 tttagaatac atttgcattt caataattta ctaactttct aatgaatcga ttcgatttag
    12181 gtgttcacct cagagctgcc agtttttatg agggaggccc gaagtcgact ttatcgctgt
    12241 gacacatact ttctgggcaa aacgattgcc gaattgccgc tttttctcac agtgccactg
    12301 gtcttcacgg cgattgccta tccgatgatc ggactgcggg ccggagtgct gcacttcttc
    12361 aactgcctgg cgctggtcac tctggtggcc aatgtgtcaa cgtccttcgg atatctaata
    12421 tcctgcgcca gctcctcgac ctcgatggcg ctgtctgtgg gtccgccggt tatcatacca
    12481 ttcctgctct ttggcggctt cttcttgaac tcgggctcgg tgccagtata cctcaaatgg
    12541 ttgtcgtacc tctcatggtt ccgttacgcc aacgagggtc tgctgattaa ccaatgggcg
    12601 gacgtggagc cgggcgaaat tagctgcaca tcgtcgaaca ccacgtgccc cagttcgggc
    12661 aaggtcatcc tggagacgct taacttctcc gccgccgatc tgccgctgga ctacgtgggt
    12721 ctggccattc tcatcgtgag cttccgggtg ctcgcatatc tggctctaag acttcgggcc
    12781 cgacgcaagg agtagccgac atatatccga aataactgct tgtttttttt ttttaccatt
    12841 attaccatcg tgtttactgt ttattgcccc ctcaaaaagc taatgtaatt atatttgtgc
    12901 caataaaaac aagatatgac ctatagaata caagtatttc cccttcgaac atccccacaa
    12961 gtagactttg gatttgtctt ctaaccaaaa gacttacaca cctgcatacc ttacatcaaa
    13021 aactcgttta tcgctacata aaacaccggg atatattttt tatatacata cttttcaaat
    13081 cgcgcgccct cttcataatt cacctccacc acaccacgtt tcgtagttgc tctttcgctg
    13141 tctcccaccc gctctccgca acacattcac cttttgttcg acgaccttgg agcgactgtc
    13201 gttagttccg cgcgattcgg ttcgctcaaa tggttccgag tggttcattt cgtctcaata
    13261 gaaattagta ataaatattt gtatgtacaa tttatttgct ccaatatatt tgtatatatt
    13321 tccctcacag ctatatttat tctaatttaa tattatgact ttttaaggta attttttgtg
    13381 acctgttcgg agtgattagc gttacaattt gaactgaaag tgacatccag tgtttgttcc
    13441 ttgtgtagat gcatctcaaa aaaatggtgg gcataatagt gttgtttata tatatcaaaa
    13501 ataacaacta taataataag aatacattta atttagaaaa tgcttggatt tcactggaac
    13561 tagaattaat tcggctgctg ctctaaacga cgcatttcgt actccaaagt acgaattttt
    13621 tccctcaagc tcttattttc attaaacaat gaacaggacc taacgcacag tcacgttatt
    13681 gtttacataa atgatttttt ttactattca aacttactct gtttgtgtac tcccactggt
    13741 atagccttct tttatctttt ctggttcagg ctctatcact ttactaggta cggcatctgc
    13801 gttgagtcgc ctccttttaa atgtctgacc ttttgcaggt gcagccttcc actgcgaatc
    13861 attaaagtgg gtatcacaaa tttgggagtt ttcaccaagg ctgcacccaa ggctctgctc
    13921 ccacaatttt ctcttaatag cacacttcgg cacgtgaatt aattttactc cagtcacagc
    13981 tttgcagcaa aatttgcaat atttcatttt tttttattcc acgtaagggt taatgttttc
    14041 aaaaaaaaat tcgtccgcac acaacctttc ctctcaacaa gcaaacgtgc actgaattta
    14101 agtgtatact tcggtaagct tcggctatcg acgggaccac cttatgttat ttcatcatgg
    14161 gccagaccca cgtagtccag cggcagatcg gcggcggaga agttaagcgt ctccaggatg
    14221 accttgcccg aactggggca cgtggtgttc gacgatgtgc agctaatttc gcccggctcc
    14281 acgtccgccc attggttaat cagcagaccc tcgttggcgt aacggaacca tgagaggtac
    14341 gacaaccatt tgaggtatac tggcaccgag cccgagttca agaagaaggc gtttttccat
    14401 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
    14461 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
    14521 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
    14581 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
    14641 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    14701 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    14761 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    14821 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    14881 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    14941 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    15001 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
    15061 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
    15121 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    15181 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    15241 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    15301 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    15361 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    15421 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    15481 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    15541 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    15601 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    15661 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    15721 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    15781 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    15841 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    15901 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    15961 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    16021 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    16081 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    16141 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
    16201 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
    16261 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
    16321 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
    16381 gtactgagag tgcaccatat cgacatgccc gccgtgaccg tcgagaaccc gctgacgctg
    16441 ccccgcgtat ccgcacccgc cgacgccgtc gcacgtcccg tgctcaccgt gaccaccgcg
    16501 cccagcggtt tcgagggcga gggcttcccg gtgcgccgcg cgttcgccgg gatcaactac
    16561 cgccacctcg acccgttcat catgatggac cagatgggtg aggtggagta cgcgcccggg
    16621 gagcccaagg gcacgccctg gcacccgcac cgcggcttcg agaccgtgac ctacatcgtc
    16681 gatatgcggt gtgaaatacc gcaccgaatc gcgcggaact aacgacagtc gctccaaggt
    16741 cgtcgaacaa aaggtgaatg tgttgcggag agcgggtggg agacagcgaa agagcaacta
    16801 cgaaacgtgg tgtggtggag gtgaattatg aagagggcgc gcgatttgaa aagtatgtat
    16861 ataaaaaata tatcccggtg ttttatgtag cgataaacga gtttttgatg taaggtatgc
    16921 aggtgtgtaa gtcttttggt tagaagacaa atccaaagtc tacttgtggg gatgttcgaa
    16981 ggggaaatac ttgtattcta taggtcatat cttgttttta ttggcacaaa tataattaca
    17041 ttagcttttt gagggggcaa taaacagtaa acacgatggt aataatggta aaaaaaaaaa
    17101 acaagcagtt atttcggata tatgtcggct actccttgcg tcgggcccga agtcttagag
    17161 ccagatatgc gagcacccgg aagctcacga tgagaatggc cagac
//
