LOCUS       sequence-381839-        5285 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synGR Cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5285)
  AUTHORS   Sanford A, Kiriakov S, Khalil AS
  TITLE     A Toolkit for Precise, Multigene Control in Saccharomyces 
            cerevisiae.
  JOURNAL   ACS Synth Biol. 2022 Nov 11. doi: 10.1021/acssynbio.2c00423.
  PUBMED    36367334
REFERENCE   2  (bases 1 to 5285)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2022 Nov 11. doi: 10.1021/acssynbio.2c00423."
COMMENT     Sequence Label: pAS162
FEATURES             Location/Qualifiers
     source          1..5285
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        492..905
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     terminator      3038..3225
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3093..3117)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(join(3542..4333,4334..4402))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4165..4184
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4403..4507)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4610..5198
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5099..5118
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtggataacc gtgcggccgc ccctgaattc gcatctagaa cggggtcatc acggctcatc
       61 atgcgccaaa caaatgtgtg caatacacgc tcggatgact gcatgatgac cgcactgact
      121 ggggacagca gatccaccta agcctgtgag agaagcagac acccgacaga tcaaggcagt
      181 tacgtctcac caaaccagat gtcaacacag ctacaacgcc ttgccaacag ggagttcttc
      241 agagacatgg aggctcaaaa cgaaattatt gacagcctag acatcaatag tcatacaaca
      301 gaaagcgacc acccaacttt ggctgataat agcgtataaa caatgcatac tttgtacgtt
      361 caaaatacaa tgcagtagat atatttatgc atattacata taatacatat cacataggaa
      421 gcaacaggcg cgttggactt ttaattttcg aggaccgcga atccttacat cacacccaat
      481 cccccacaag tgatccccca cacaccatag cttcaaaatg tttctactcc ttttttactc
      541 ttccagattt tctcggactc cgcgcatcgc cgtaccactt caaaacaccc aagcacagca
      601 tactaaattt cccctctttc ttcctctagg gtgtcgttaa ttacccgtac taaaggtttg
      661 gaaaagaaaa aagacaccgc ctcgtttctt tttcttcgtc gaaaaaggca ataaaaattt
      721 ttatcacgtt tctttttctt gaaaattttt ttttttgatt tttttctctt tcgatgacct
      781 cccattgata tttaagttaa taaacggtca tcaatttctc aagtttcagt ttcatttttc
      841 ttgttctatt acaacttttt ttacttcttg ctcattagaa agaaagcata gcaatctaat
      901 ctaagtttta attacaaaag atctatgctc gagaaaaaaa tggagcgccc cttccagtgt
      961 cgcatttgca tgcggaactt ttcgagaaat ttcattttgc aaagacatac ccgtactcat
     1021 accggtgaaa aaccgtttca gtgtcggatc tgtatgcgaa atttctccga tagagctaat
     1081 ttgagaagac atctacgtac gcacaccggc gagaagccat tccaatgccg aatatgcatg
     1141 cgcaacttca gtagacatga tcaattgact agacacctaa aaacccacct gagaggagat
     1201 ggtgctggat tgattgatat cgataacctg gaagctcgaa aaacaaagaa aaaaataaaa
     1261 ggaattcagc aggccactac aggagtctca caagaaacct ctgaaaatcc tggtaacaaa
     1321 acaatagttc ctgcaacgtt accacaactc acccctaccc tggtgtcact gttggaggtt
     1381 attgaacctg aagtgttata tgcaggatat gatagctctg ttccagactc aacttggagg
     1441 atcatgacta cgctcaacat gttaggaggg cggcaagtga ttgcagcagt gaaatgggca
     1501 aaggcaatac caggtttcag gaacttacac ctggatgacc aaatgaccct actgcagtac
     1561 tcctggatgt ttcttatggc atttgctctg gggtggagat catatagaca accaagtgca
     1621 aacctgctgt gttttgctcc tgatctgatt attaatgagc agagaatgac tctaccctgc
     1681 atgtacgacc aatgtaaaca catgctgtat gtttcctctg agttacacag gcttcaggta
     1741 tcttatgaag agtatctctg tatgaaaacc ttactgcttc tctcttcagt tcctaaggac
     1801 ggtctgaaga gccaagagct atttgatgaa attagaatga cctacatcaa agagctagga
     1861 aaagccattg tcaagaggga aggaaactcc agccagaact ggcagcggtt ttatcaactg
     1921 acaaaactct tggattctat gcatgaagtg gttgaaaatc tccttaacta ttgcttccaa
     1981 acatttttgg ataagaccat gagtattgaa ttccccgaga tgttagctga aatcatcacc
     2041 aatcagatac caaaatactc aaacggaaat atcaaaaaac ttctgtttca tcaaaagggc
     2101 gacggagcag gcttaataaa tgctagcatg acggtcgacc atgatttcaa tagcgaagat
     2161 attttattcc ccatagaaag catgagtagt atacaatacg tggagaataa taacccaaat
     2221 aatattaaca acgatgttat cccgtattct ctagatatca aaaacactgt cttagatagt
     2281 gcggatctca atgacattca aaatcaagaa acttcactga atttggggct tcctccacta
     2341 tctttcgact ctccactgcc cgtaacggaa acgataccat ccactaccga taacagcttg
     2401 catttgaaag ctgatagcaa caaaaatcgc gatgcaagaa ctattgaaaa tgatagtgaa
     2461 attaagagta ctaataatgc tagtggctct ggggcaaatc aatacacaac tcttacttca
     2521 ccttatccta tgaacgacat tttgtacaac atgaacaatc cgttacaatc accgtcacct
     2581 tcatcggtac ctcaaaatcc gactataaat cctcccataa atacagcaag taacgaaact
     2641 aatttatcgc ctcaaacttc aaatggtaat gaaactctta tatctccacg agcccaacaa
     2701 catacgtcca ttaaagataa tcgtctgtcc ttacctaatg gtgctaattc gaatcttttc
     2761 attgacacta acccaaacaa tttgaacgaa aaactaagaa atcaattgaa ctcagataca
     2821 aattcatatt ctaactccat ttctaattca aactccaatt ctacgggtaa tttaaattcc
     2881 agttatttta attcactgaa catagactcc atgctagatg attacgtttc tagtgatctc
     2941 ttattgaatg atgatgatga tgacactaat ttatcacgcc gaagatttag cgacgttata
     3001 acaaaccaag agctctaagg gcccatccta actcgaggcg aatttcttat gatttatgat
     3061 ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta
     3121 ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca ggttgctttc
     3181 tcaggtatag catgaggtcg ctcttattga ccacacctct accggcatgc cgagcaaatg
     3241 cctgcaaatc gctccccatt tcgctgagca tgagacggaa atctgctcgt cagtggtgct
     3301 cacactgacg aatcatgtac agatcatacc gatgactgcc tggcgactca caactaagca
     3361 agacagccgg aaccagcgcc ggcgaacacc actgcatata tggcatatca caacagtcca
     3421 actagtgcac tgcagtacag cggccgcgat tatcaaaaag gatcttcacc tagatccttt
     3481 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     3541 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     3601 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     3661 ccagtgctgc aatgataccg cgggacccac gctcaccggc tccagattta tcagcaataa
     3721 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     3781 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     3841 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     3901 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     3961 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     4021 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     4081 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     4141 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     4201 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     4261 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     4321 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     4381 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     4441 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     4501 ttccgcgcac atttccccga aaagtgccac ctgtcatgac caaaatccct taacgtgagt
     4561 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     4621 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     4681 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     4741 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     4801 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     4861 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     4921 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     4981 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     5041 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     5101 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     5161 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     5221 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     5281 attct
//
