LOCUS       sequence-38190-e         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 3347 (=HL 3338 + pHL 720)
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    229..245
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        303..407
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(626..645)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cttctccttt acgcatgctc gcggtacctt taatcaggtt gttcaggtta gtgacataag
       61 tctgatggtg cttgccgtag tgatactcga tggtttccgc agaaatgtgc ggtgccagag
      121 catctttagc atatggtagt gcaggtaatt cgaatgacat tgctactctc ctttattatt
      181 aatttgcata cgtacaatag ccttattgtg cgtatggtcg acagtatctt gttatccgct
      241 cacaatgtca atgttatccg ctcacattta tgacgtcagg tggcactttt cggggaaatg
      301 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      361 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      421 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      481 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      541 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      601 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
      661 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
      721 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
      781 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
      841 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgtt
//
