LOCUS       sequence-381903-        2089 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  97-4 Op (Part 2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Sanford A, Kiriakov S, Khalil AS
  TITLE     A Toolkit for Precise, Multigene Control in Saccharomyces 
            cerevisiae.
  JOURNAL   ACS Synth Biol. 2022 Nov 11. doi: 10.1021/acssynbio.2c00423.
  PUBMED    36367334
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2022 Nov 11. doi: 10.1021/acssynbio.2c00423."
COMMENT     Sequence Label: pAS165
FEATURES             Location/Qualifiers
     source          1..2089
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(455..489)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(560..1219)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1134..1153
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1401..1989
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1890..1909
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtcggtctca aacgcctagg ttgaggccaa cgattatggg agatcgaata ttaatattat
       61 gggagatcga atattaatat tatgggagat cgaatattaa tattatggga gatcgaatat
      121 taatattatg ggagatcgaa tattaatatt atgggagatc gaatattaat attatgggag
      181 atggcatgaa tggccttaag cagatccgcc aggcgtgtat atatagcgtg gatggccagg
      241 caactttagt gctgacacat acaggcatat atatatgtgt gcgacgacac atgatcatat
      301 ggcatgcatg tgctctgtat gtatataaaa ctcttgtttt cttcttttct ctaaatattc
      361 tttccttata cattaggacc tttgcagcat aaattactat acttctatag acacacaaac
      421 acaaatacac acactaatat gtgagaccag accaataaaa aacgcccggc ggcaaccgag
      481 cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc
      541 aagcgagctc gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt
      601 cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca
      661 gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga
      721 agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg
      781 ctgaaacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt
      841 aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac
      901 tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac
      961 tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca
     1021 tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg
     1081 tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg
     1141 actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc
     1201 cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa
     1261 atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgcccga
     1321 tcaatcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     1381 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     1441 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     1501 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     1561 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     1621 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     1681 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     1741 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     1801 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     1861 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     1921 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     1981 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     2041 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgta
//
