LOCUS       sequence-381908-        2164 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  43-8 Op (Part 2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Sanford A, Kiriakov S, Khalil AS
  TITLE     A Toolkit for Precise, Multigene Control in Saccharomyces 
            cerevisiae.
  JOURNAL   ACS Synth Biol. 2022 Nov 11. doi: 10.1021/acssynbio.2c00423.
  PUBMED    36367334
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2022 Nov 11. doi: 10.1021/acssynbio.2c00423."
COMMENT     Sequence Label: pAS166
FEATURES             Location/Qualifiers
     source          1..2164
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(529..563)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(634..1293)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1208..1227
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1475..2063
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1964..1983
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acgcctagga ttttctttaa ttaaagagtg aggactcgaa aatattaata
       61 gagtgaggac tcgaaaatat taatagagtg aggactcgaa aatattaata gagtgaggac
      121 tcgaaaatat taatatcgat agagtgagga ctcgaaaata ttaatagagt gaggactcga
      181 aaatattaat gaattcagag tgaggactcg aaaatattaa tagagtgagg actcgaaaat
      241 attaatggcc ggccccttaa gcagatccgc caggcagatc cgccaggcgt gtatatatag
      301 cgtggatggc caggcaactt tagtgctgac acatacaggc atatatatat gtgtgcgacg
      361 acacatgatc atatggcatg catgtgctct gtatgtatat aaaactcttg ttttcttctt
      421 ttctctaaat attctttcct tatacattag gacctttgca gcataaatta ctatacttct
      481 atagacacac aaacacaaat acacacacta atatgtgaga ccagaccaat aaaaaacgcc
      541 cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct
      601 atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcatcgca
      661 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg
      721 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt
      781 gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact
      841 cacccaggga ttggctgaaa cgaaaaacat attctcaata aaccctttag ggaaataggc
      901 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc
      961 gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta
     1021 acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc
     1081 cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt
     1141 atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt
     1201 acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc
     1261 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct
     1321 cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc
     1381 tcttacgtgc ccgatcaatc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     1441 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     1501 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     1561 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     1621 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     1681 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     1741 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     1801 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     1861 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     1921 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     1981 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     2041 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     2101 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     2161 gtag
//
