LOCUS       sequence-381910-       14927 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EF1a-dCas9-KRAB-GFP with nontarget sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14927)
  AUTHORS   Rieskamp JD, Rosado-Burgos I, Christofi JE, Ansar E, Einstein D, 
            Walters AE, Valentini V, Bruno JP, Kirby ED
  TITLE     Excitatory amino acid transporter 1 supports adult hippocampal 
            neural stem cell self-renewal.
  JOURNAL   iScience. 2023 Jun 8;26(7):107068. doi: 10.1016/j.isci.2023.107068. 
            eCollection 2023 Jul 21.
  PUBMED    37534178
REFERENCE   2  (bases 1 to 14927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2023-06-8"; volume: "26(7):107068. doi"; pages: " 
            10.1016/j.isci.2023.107068. eCollection 2023 Jul 21"
COMMENT     Sequence Label: U6-NT sgRNA; EF1a-dCas9-KRAB-GFP
FEATURES             Location/Qualifiers
     source          1..14927
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2364..2604
                     /label=U6 promoter
                     /note="Truncated RNA polymerase III promoter for human U6 
                     snRNA used in pSIREN vectors such as 
                     pSIREN-RetroQ-ZsGreen1. Expected transcription start site 
                     is 8bp downstream of this feature."
     primer_bind     2364..2384
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2535..2554
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2626..2701
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2745..3923
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2976..3914
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3871..3891
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     3940..3959
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3940..3958
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4000..4009
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4009..4074
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4081..4101
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(4111..4137,4138..4140,4141..6624,6625..6630,6631..6696,
                     6697..6699,6700..8214)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(4111..4137,4138..4140,4141..6627,6628..6630,
                     6631..8214)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4111..8214
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8227..8247
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8281..8475
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQILYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     CDS             8542..8562
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8569..8622
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(8629..8631,8632..9342)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(8629..8631,8632..9342)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(8671..8692)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8932..8951)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9279..9300
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    9382..9970
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9435..9455)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9853..9864)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9973..9989)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9974..9990)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10495..10675
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10701..10718)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10707..10931
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10977..11405
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11064..11083)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11274..11295
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11414..11434)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11419..11748
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11599..11734
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11661..11680
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        11796..11843
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             11862..12236
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    12366..12487
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12403..12422)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12457..12476
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12536..12552)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12536..12552)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12549..12571)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12560..12576
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12584..12590,12591..12608,12609..12614))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12629..12650
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12767..12784)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12938..13526)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13018..13037)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13697..14488,14489..14557))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14320..14339
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14558..14662)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(14737..14756)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttagagggcc tatttcccat gattccttca tatttgcata
     2401 tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat
     2461 tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat
     2521 tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg
     2581 ctttatatat cttgtggaaa ggacggcgag gtattcggct ccgcggtttt agagctagaa
     2641 atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg
     2701 cttttttgtt ttttaattaa ttaacccgtg tcggctccag atctggctcc ggtgcccgtc
     2761 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
     2821 gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
     2881 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
     2941 ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg
     3001 ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc
     3061 agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt
     3121 gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc
     3181 gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag
     3241 ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa
     3301 atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg
     3361 cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa
     3421 tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggtctc gcgccgccgt
     3481 gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa
     3541 gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag
     3601 agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt
     3661 catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt
     3721 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg
     3781 agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg
     3841 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt
     3901 ttcttccatt tcaggtgtcg tgaggaatta gcttggtact aatacgactc actataggga
     3961 gacccaagct gcgtacgtgg gctgcaggtc gactctagag ccaccatgga ctacaaagac
     4021 catgacggtg attataaaga tcatgacatc gattacaagg atgacgatga caagatggcc
     4081 cccaagaaga agaggaaggt gggccgcgga atggacaaga agtactccat tgggctcgcc
     4141 atcggcacaa acagcgtcgg ctgggccgtc attacggacg agtacaaggt gccgagcaaa
     4201 aaattcaaag ttctgggcaa taccgatcgc cacagcataa agaagaacct cattggcgcc
     4261 ctcctgttcg actccgggga aaccgccgaa gccacgcggc tcaaaagaac agcacggcgc
     4321 agatataccc gcagaaagaa tcggatctgc tacctgcagg agatctttag taatgagatg
     4381 gctaaggtgg atgactcttt cttccatagg ctggaggagt cctttttggt ggaggaggat
     4441 aaaaagcacg agcgccaccc aatctttggc aatatcgtgg acgaggtggc gtaccatgaa
     4501 aagtacccaa ccatatatca tctgaggaag aagcttgtag acagtactga taaggctgac
     4561 ttgcggttga tctatctcgc gctggcgcat atgatcaaat ttcggggaca cttcctcatc
     4621 gagggggacc tgaacccaga caacagcgat gtcgacaaac tctttatcca actggttcag
     4681 acttacaatc agcttttcga agagaacccg atcaacgcat ccggagttga cgccaaagca
     4741 atcctgagcg ctaggctgtc caaatcccgg cggctcgaaa acctcatcgc acagctccct
     4801 ggggagaaga agaacggcct gtttggtaat cttatcgccc tgtcactcgg gctgaccccc
     4861 aactttaaat ctaacttcga cctggccgaa gatgccaagc ttcaactgag caaagacacc
     4921 tacgatgatg atctcgacaa tctgctggcc cagatcggcg accagtacgc agaccttttt
     4981 ttggcggcaa agaacctgtc agacgccatt ctgctgagtg atattctgcg agtgaacacg
     5041 gagatcacca aagctccgct gagcgctagt atgatcaagc gctatgatga gcaccaccaa
     5101 gacttgactt tgctgaaggc ccttgtcaga cagcaactgc ctgagaagta caaggaaatt
     5161 ttcttcgatc agtctaaaaa tggctacgcc ggatacattg acggcggagc aagccaggag
     5221 gaattttaca aatttattaa gcccatcttg gaaaaaatgg acggcaccga ggagctgctg
     5281 gtaaagctta acagagaaga tctgttgcgc aaacagcgca ctttcgacaa tggaagcatc
     5341 ccccaccaga ttcacctggg cgaactgcac gctatcctca ggcggcaaga ggatttctac
     5401 ccctttttga aagataacag ggaaaagatt gagaaaatcc tcacatttcg gataccctac
     5461 tatgtaggcc ccctcgcccg gggaaattcc agattcgcgt ggatgactcg caaatcagaa
     5521 gagaccatca ctccctggaa cttcgaggaa gtcgtggata agggggcctc tgcccagtcc
     5581 ttcatcgaaa ggatgactaa ctttgataaa aatctgccta acgaaaaggt gcttcctaaa
     5641 cactctctgc tgtacgagta cttcacagtt tataacgagc tcaccaaggt caaatacgtc
     5701 acagaaggga tgagaaagcc agcattcctg tctggagagc agaagaaagc tatcgtggac
     5761 ctcctcttca agacgaaccg gaaagttacc gtgaaacagc tcaaagaaga ctatttcaaa
     5821 aagattgaat gtttcgactc tgttgaaatc agcggagtgg aggatcgctt caacgcatcc
     5881 ctgggaacgt atcacgatct cctgaaaatc attaaagaca aggacttcct ggacaatgag
     5941 gagaacgagg acattcttga ggacattgtc ctcaccctta cgttgtttga agatagggag
     6001 atgattgaag aacgcttgaa aacttacgct catctcttcg acgacaaagt catgaaacag
     6061 ctcaagaggc gccgatatac aggatggggg cggctgtcaa gaaaactgat caatgggatc
     6121 cgagacaagc agagtggaaa gacaatcctg gattttctta agtccgatgg atttgccaac
     6181 cggaacttca tgcagttgat ccatgatgac tctctcacct ttaaggagga catccagaaa
     6241 gcacaagttt ctggccaggg ggacagtctt cacgagcaca tcgctaatct tgcaggtagc
     6301 ccagctatca aaaagggaat actgcagacc gttaaggtcg tggatgaact cgtcaaagta
     6361 atgggaaggc ataagcccga gaatatcgtt atcgagatgg cccgagagaa ccaaactacc
     6421 cagaagggac agaagaacag tagggaaagg atgaagagga ttgaagaggg tataaaagaa
     6481 ctggggtccc aaatccttaa ggaacaccca gttgaaaaca cccagcttca gaatgagaag
     6541 ctctacctgt actacctgca gaacggcagg gacatgtacg tggatcagga actggacatc
     6601 aatcggctct ccgactacga cgtggatgcc atcgtgcccc agtcttttct caaagatgat
     6661 tctattgata ataaagtgtt gacaagatcc gataaaaata gagggaagag tgataacgtc
     6721 ccctcagaag aagttgtcaa gaaaatgaaa aattattggc ggcagctgct gaacgccaaa
     6781 ctgatcacac aacggaagtt cgataatctg actaaggctg aacgaggtgg cctgtctgag
     6841 ttggataaag ccggcttcat caaaaggcag cttgttgaga cacgccagat caccaagcac
     6901 gtggcccaaa ttctcgattc acgcatgaac accaagtacg atgaaaatga caaactgatt
     6961 cgagaggtga aagttattac tctgaagtct aagctggtct cagatttcag aaaggacttt
     7021 cagttttata aggtgagaga gatcaacaat taccaccatg cgcatgatgc ctacctgaat
     7081 gcagtggtag gcactgcact tatcaaaaaa tatcccaagc ttgaatctga atttgtttac
     7141 ggagactata aagtgtacga tgttaggaaa atgatcgcaa agtctgagca ggaaataggc
     7201 aaggccaccg ctaagtactt cttttacagc aatattatga attttttcaa gaccgagatt
     7261 acactggcca atggagagat tcggaagcga ccacttatcg aaacaaacgg agaaacagga
     7321 gaaatcgtgt gggacaaggg tagggatttc gcgacagtcc ggaaggtcct gtccatgccg
     7381 caggtgaaca tcgttaaaaa gaccgaagta cagaccggag gcttctccaa ggaaagtatc
     7441 ctcccgaaaa ggaacagcga caagctgatc gcacgcaaaa aagattggga ccccaagaaa
     7501 tacggcggat tcgattctcc tacagtcgct tacagtgtac tggttgtggc caaagtggag
     7561 aaagggaagt ctaaaaaact caaaagcgtc aaggaactgc tgggcatcac aatcatggag
     7621 cgatcaagct tcgaaaaaaa ccccatcgac tttctcgagg cgaaaggata taaagaggtc
     7681 aaaaaagacc tcatcattaa gcttcccaag tactctctct ttgagcttga aaacggccgg
     7741 aaacgaatgc tcgctagtgc gggcgagctg cagaaaggta acgagctggc actgccctct
     7801 aaatacgtta atttcttgta tctggccagc cactatgaaa agctcaaagg gtctcccgaa
     7861 gataatgagc agaagcagct gttcgtggaa caacacaaac actaccttga tgagatcatc
     7921 gagcaaataa gcgaattctc caaaagagtg atcctcgccg acgctaacct cgataaggtg
     7981 ctttctgctt acaataagca cagggataag cccatcaggg agcaggcaga aaacattatc
     8041 cacttgttta ctctgaccaa cttgggcgcg cctgcagcct tcaagtactt cgacaccacc
     8101 atagacagaa agcggtacac ctctacaaag gaggtcctgg acgccacact gattcatcag
     8161 tcaattacgg ggctctatga aacaagaatc gacctctctc agctcggtgg agacagcagg
     8221 gctgacccca agaagaagag gaaggtggct agcgatgcta agtcactgac tgcctggtcc
     8281 cggacactgg tgaccttcaa ggatgtgttt gtggacttca ccagggagga gtggaagctg
     8341 ctggacactg ctcagcagat cctgtacaga aatgtgatgc tggagaacta taagaacctg
     8401 gtttccttgg gttatcagct tactaagcca gatgtgatcc tccggttgga gaagggagaa
     8461 gagccctggc tggtggagag agaaattcac caagagaccc atcctgattc agagactgca
     8521 tttgaaatca aatcatcagt tccgaaaaag aaacgcaaag ttgctagcga gggcagagga
     8581 agtcttctaa catgcggtga cgtggaggag aatcccggcc ctggtaccgt gagcaagggc
     8641 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     8701 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     8761 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     8821 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     8881 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     8941 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     9001 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     9061 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     9121 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     9181 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     9241 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     9301 accgccgccg ggatcactct cggcatggac gagctgtaca agaccggttg ataataggaa
     9361 ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact
     9421 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     9481 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     9541 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     9601 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     9661 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     9721 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     9781 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     9841 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     9901 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     9961 gcctccccgc atcgataccg tcgacctcga gacctagaaa aacatggagc aatcacaagt
    10021 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag
    10081 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta
    10141 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
    10201 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag
    10261 aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta
    10321 gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta
    10381 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt
    10441 gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct
    10501 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
    10561 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
    10621 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc
    10681 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
    10741 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
    10801 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
    10861 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
    10921 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
    10981 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
    11041 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
    11101 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
    11161 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
    11221 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
    11281 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
    11341 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
    11401 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
    11461 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
    11521 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
    11581 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
    11641 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
    11701 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
    11761 gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt
    11821 atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg
    11881 ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc
    11941 tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga
    12001 ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt
    12061 gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc
    12121 gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg
    12181 ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg
    12241 tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
    12301 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
    12361 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
    12421 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
    12481 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
    12541 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
    12601 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
    12661 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
    12721 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
    12781 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
    12841 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
    12901 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
    12961 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
    13021 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
    13081 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
    13141 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
    13201 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    13261 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    13321 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
    13381 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
    13441 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    13501 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    13561 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
    13621 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
    13681 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
    13741 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
    13801 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
    13861 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    13921 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    13981 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    14041 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    14101 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    14161 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    14221 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    14281 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    14341 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    14401 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    14461 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    14521 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    14581 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    14641 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
    14701 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata
    14761 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
    14821 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt
    14881 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgtt
//
