LOCUS       sequence-381912-       14920 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for CRISPRi-GFP expression ready for gateway cloning of 
            sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14920)
  AUTHORS   Rieskamp JD, Rosado-Burgos I, Christofi JE, Ansar E, Einstein D, 
            Walters AE, Valentini V, Bruno JP, Kirby ED
  TITLE     Excitatory amino acid transporter 1 supports adult hippocampal 
            neural stem cell self-renewal.
  JOURNAL   iScience. 2023 Jun 8;26(7):107068. doi: 10.1016/j.isci.2023.107068. 
            eCollection 2023 Jul 21.
  PUBMED    37534178
REFERENCE   2  (bases 1 to 14920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2023-06-8"; volume: "26(7):107068. doi"; pages: " 
            10.1016/j.isci.2023.107068. eCollection 2023 Jul 21"
COMMENT     Sequence Label: EF1a-dCas9-KRAB-GFP backbone
FEATURES             Location/Qualifiers
     source          1..14920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2364..2604
                     /label=U6 promoter
                     /note="Truncated RNA polymerase III promoter for human U6 
                     snRNA used in pSIREN vectors such as 
                     pSIREN-RetroQ-ZsGreen1. Expected transcription start site 
                     is 8bp downstream of this feature."
     primer_bind     2364..2384
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2535..2554
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2619..2694
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2738..3916
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2969..3907
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3864..3884
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     3933..3952
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3933..3951
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3993..4002
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4002..4067
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4074..4094
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(4104..4130,4131..4133,4134..6617,6618..6623,6624..6689,
                     6690..6692,6693..8207)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(4104..4130,4131..4133,4134..6620,6621..6623,
                     6624..8207)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4104..8207
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8220..8240
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8274..8468
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQILYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     CDS             8535..8555
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8562..8615
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(8622..8624,8625..9335)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(8622..8624,8625..9335)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(8664..8685)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8925..8944)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9272..9293
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    9375..9963
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9428..9448)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9846..9857)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9966..9982)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9967..9983)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10488..10668
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10694..10711)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10700..10924
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10970..11398
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11057..11076)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11267..11288
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11407..11427)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11412..11741
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11592..11727
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11654..11673
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        11789..11836
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             11855..12229
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    12359..12480
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12396..12415)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12450..12469
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12529..12545)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12529..12545)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12542..12564)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12553..12569
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12577..12583,12584..12601,12602..12607))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12622..12643
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12760..12777)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12931..13519)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13011..13030)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13690..14481,14482..14550))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14313..14332
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14551..14655)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(14730..14749)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttagagggcc tatttcccat gattccttca tatttgcata
     2401 tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat
     2461 tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat
     2521 tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg
     2581 ctttatatat cttgtggaaa ggactgagac gcgtctcagt tttagagcta gaaatagcaa
     2641 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt
     2701 gttttttaat taattaaccc gtgtcggctc cagatctggc tccggtgccc gtcagtgggc
     2761 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
     2821 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     2881 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     2941 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
     3001 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
     3061 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
     3121 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
     3181 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
     3241 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
     3301 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
     3361 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
     3421 ggggtagtct caagctggcc ggcctgctct ggtgcctggt ctcgcgccgc cgtgtatcgc
     3481 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
     3541 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
     3601 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     3661 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     3721 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     3781 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     3841 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     3901 atttcaggtg tcgtgaggaa ttagcttggt actaatacga ctcactatag ggagacccaa
     3961 gctgcgtacg tgggctgcag gtcgactcta gagccaccat ggactacaaa gaccatgacg
     4021 gtgattataa agatcatgac atcgattaca aggatgacga tgacaagatg gcccccaaga
     4081 agaagaggaa ggtgggccgc ggaatggaca agaagtactc cattgggctc gccatcggca
     4141 caaacagcgt cggctgggcc gtcattacgg acgagtacaa ggtgccgagc aaaaaattca
     4201 aagttctggg caataccgat cgccacagca taaagaagaa cctcattggc gccctcctgt
     4261 tcgactccgg ggaaaccgcc gaagccacgc ggctcaaaag aacagcacgg cgcagatata
     4321 cccgcagaaa gaatcggatc tgctacctgc aggagatctt tagtaatgag atggctaagg
     4381 tggatgactc tttcttccat aggctggagg agtccttttt ggtggaggag gataaaaagc
     4441 acgagcgcca cccaatcttt ggcaatatcg tggacgaggt ggcgtaccat gaaaagtacc
     4501 caaccatata tcatctgagg aagaagcttg tagacagtac tgataaggct gacttgcggt
     4561 tgatctatct cgcgctggcg catatgatca aatttcgggg acacttcctc atcgaggggg
     4621 acctgaaccc agacaacagc gatgtcgaca aactctttat ccaactggtt cagacttaca
     4681 atcagctttt cgaagagaac ccgatcaacg catccggagt tgacgccaaa gcaatcctga
     4741 gcgctaggct gtccaaatcc cggcggctcg aaaacctcat cgcacagctc cctggggaga
     4801 agaagaacgg cctgtttggt aatcttatcg ccctgtcact cgggctgacc cccaacttta
     4861 aatctaactt cgacctggcc gaagatgcca agcttcaact gagcaaagac acctacgatg
     4921 atgatctcga caatctgctg gcccagatcg gcgaccagta cgcagacctt tttttggcgg
     4981 caaagaacct gtcagacgcc attctgctga gtgatattct gcgagtgaac acggagatca
     5041 ccaaagctcc gctgagcgct agtatgatca agcgctatga tgagcaccac caagacttga
     5101 ctttgctgaa ggcccttgtc agacagcaac tgcctgagaa gtacaaggaa attttcttcg
     5161 atcagtctaa aaatggctac gccggataca ttgacggcgg agcaagccag gaggaatttt
     5221 acaaatttat taagcccatc ttggaaaaaa tggacggcac cgaggagctg ctggtaaagc
     5281 ttaacagaga agatctgttg cgcaaacagc gcactttcga caatggaagc atcccccacc
     5341 agattcacct gggcgaactg cacgctatcc tcaggcggca agaggatttc tacccctttt
     5401 tgaaagataa cagggaaaag attgagaaaa tcctcacatt tcggataccc tactatgtag
     5461 gccccctcgc ccggggaaat tccagattcg cgtggatgac tcgcaaatca gaagagacca
     5521 tcactccctg gaacttcgag gaagtcgtgg ataagggggc ctctgcccag tccttcatcg
     5581 aaaggatgac taactttgat aaaaatctgc ctaacgaaaa ggtgcttcct aaacactctc
     5641 tgctgtacga gtacttcaca gtttataacg agctcaccaa ggtcaaatac gtcacagaag
     5701 ggatgagaaa gccagcattc ctgtctggag agcagaagaa agctatcgtg gacctcctct
     5761 tcaagacgaa ccggaaagtt accgtgaaac agctcaaaga agactatttc aaaaagattg
     5821 aatgtttcga ctctgttgaa atcagcggag tggaggatcg cttcaacgca tccctgggaa
     5881 cgtatcacga tctcctgaaa atcattaaag acaaggactt cctggacaat gaggagaacg
     5941 aggacattct tgaggacatt gtcctcaccc ttacgttgtt tgaagatagg gagatgattg
     6001 aagaacgctt gaaaacttac gctcatctct tcgacgacaa agtcatgaaa cagctcaaga
     6061 ggcgccgata tacaggatgg gggcggctgt caagaaaact gatcaatggg atccgagaca
     6121 agcagagtgg aaagacaatc ctggattttc ttaagtccga tggatttgcc aaccggaact
     6181 tcatgcagtt gatccatgat gactctctca cctttaagga ggacatccag aaagcacaag
     6241 tttctggcca gggggacagt cttcacgagc acatcgctaa tcttgcaggt agcccagcta
     6301 tcaaaaaggg aatactgcag accgttaagg tcgtggatga actcgtcaaa gtaatgggaa
     6361 ggcataagcc cgagaatatc gttatcgaga tggcccgaga gaaccaaact acccagaagg
     6421 gacagaagaa cagtagggaa aggatgaaga ggattgaaga gggtataaaa gaactggggt
     6481 cccaaatcct taaggaacac ccagttgaaa acacccagct tcagaatgag aagctctacc
     6541 tgtactacct gcagaacggc agggacatgt acgtggatca ggaactggac atcaatcggc
     6601 tctccgacta cgacgtggat gccatcgtgc cccagtcttt tctcaaagat gattctattg
     6661 ataataaagt gttgacaaga tccgataaaa atagagggaa gagtgataac gtcccctcag
     6721 aagaagttgt caagaaaatg aaaaattatt ggcggcagct gctgaacgcc aaactgatca
     6781 cacaacggaa gttcgataat ctgactaagg ctgaacgagg tggcctgtct gagttggata
     6841 aagccggctt catcaaaagg cagcttgttg agacacgcca gatcaccaag cacgtggccc
     6901 aaattctcga ttcacgcatg aacaccaagt acgatgaaaa tgacaaactg attcgagagg
     6961 tgaaagttat tactctgaag tctaagctgg tctcagattt cagaaaggac tttcagtttt
     7021 ataaggtgag agagatcaac aattaccacc atgcgcatga tgcctacctg aatgcagtgg
     7081 taggcactgc acttatcaaa aaatatccca agcttgaatc tgaatttgtt tacggagact
     7141 ataaagtgta cgatgttagg aaaatgatcg caaagtctga gcaggaaata ggcaaggcca
     7201 ccgctaagta cttcttttac agcaatatta tgaatttttt caagaccgag attacactgg
     7261 ccaatggaga gattcggaag cgaccactta tcgaaacaaa cggagaaaca ggagaaatcg
     7321 tgtgggacaa gggtagggat ttcgcgacag tccggaaggt cctgtccatg ccgcaggtga
     7381 acatcgttaa aaagaccgaa gtacagaccg gaggcttctc caaggaaagt atcctcccga
     7441 aaaggaacag cgacaagctg atcgcacgca aaaaagattg ggaccccaag aaatacggcg
     7501 gattcgattc tcctacagtc gcttacagtg tactggttgt ggccaaagtg gagaaaggga
     7561 agtctaaaaa actcaaaagc gtcaaggaac tgctgggcat cacaatcatg gagcgatcaa
     7621 gcttcgaaaa aaaccccatc gactttctcg aggcgaaagg atataaagag gtcaaaaaag
     7681 acctcatcat taagcttccc aagtactctc tctttgagct tgaaaacggc cggaaacgaa
     7741 tgctcgctag tgcgggcgag ctgcagaaag gtaacgagct ggcactgccc tctaaatacg
     7801 ttaatttctt gtatctggcc agccactatg aaaagctcaa agggtctccc gaagataatg
     7861 agcagaagca gctgttcgtg gaacaacaca aacactacct tgatgagatc atcgagcaaa
     7921 taagcgaatt ctccaaaaga gtgatcctcg ccgacgctaa cctcgataag gtgctttctg
     7981 cttacaataa gcacagggat aagcccatca gggagcaggc agaaaacatt atccacttgt
     8041 ttactctgac caacttgggc gcgcctgcag ccttcaagta cttcgacacc accatagaca
     8101 gaaagcggta cacctctaca aaggaggtcc tggacgccac actgattcat cagtcaatta
     8161 cggggctcta tgaaacaaga atcgacctct ctcagctcgg tggagacagc agggctgacc
     8221 ccaagaagaa gaggaaggtg gctagcgatg ctaagtcact gactgcctgg tcccggacac
     8281 tggtgacctt caaggatgtg tttgtggact tcaccaggga ggagtggaag ctgctggaca
     8341 ctgctcagca gatcctgtac agaaatgtga tgctggagaa ctataagaac ctggtttcct
     8401 tgggttatca gcttactaag ccagatgtga tcctccggtt ggagaaggga gaagagccct
     8461 ggctggtgga gagagaaatt caccaagaga cccatcctga ttcagagact gcatttgaaa
     8521 tcaaatcatc agttccgaaa aagaaacgca aagttgctag cgagggcaga ggaagtcttc
     8581 taacatgcgg tgacgtggag gagaatcccg gccctggtac cgtgagcaag ggcgaggagc
     8641 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     8701 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     8761 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     8821 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     8881 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     8941 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     9001 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     9061 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     9121 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     9181 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     9241 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     9301 ccgggatcac tctcggcatg gacgagctgt acaagaccgg ttgataatag gaattcgata
     9361 tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     9421 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     9481 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     9541 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     9601 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     9661 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     9721 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     9781 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     9841 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     9901 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     9961 cgcatcgata ccgtcgacct cgagacctag aaaaacatgg agcaatcaca agtagcaata
    10021 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt
    10081 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta
    10141 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
    10201 atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca
    10261 caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag
    10321 ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg
    10381 tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc
    10441 gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt
    10501 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
    10561 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
    10621 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa
    10681 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
    10741 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
    10801 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
    10861 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
    10921 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
    10981 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
    11041 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
    11101 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
    11161 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
    11221 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
    11281 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
    11341 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
    11401 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
    11461 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
    11521 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
    11581 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
    11641 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
    11701 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
    11761 tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg
    11821 catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc
    11881 ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt
    11941 ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt
    12001 catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg
    12061 cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc
    12121 ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg
    12181 cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg
    12241 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
    12301 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
    12361 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
    12421 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
    12481 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
    12541 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
    12601 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
    12661 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
    12721 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
    12781 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
    12841 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
    12901 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
    12961 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
    13021 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    13081 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
    13141 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    13201 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    13261 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    13321 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
    13381 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    13441 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    13501 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    13561 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    13621 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    13681 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    13741 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    13801 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    13861 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    13921 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    13981 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    14041 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    14101 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    14161 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    14221 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    14281 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    14341 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    14401 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    14461 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    14521 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    14581 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    14641 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
    14701 tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
    14761 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa
    14821 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt
    14881 tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
//
