LOCUS       sequence-381913-        2074 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  37-12 Op (Part 2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Sanford A, Kiriakov S, Khalil AS
  TITLE     A Toolkit for Precise, Multigene Control in Saccharomyces 
            cerevisiae.
  JOURNAL   ACS Synth Biol. 2022 Nov 11. doi: 10.1021/acssynbio.2c00423.
  PUBMED    36367334
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2022 Nov 11. doi: 10.1021/acssynbio.2c00423."
COMMENT     Sequence Label: pAS167
FEATURES             Location/Qualifiers
     source          1..2074
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(389..977)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(469..488)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1159..1818
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1225..1244)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1889..1923
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gccatatgat catgtgtcgt cgcacacata tatatatgcc tgtatgtgtc agcactaaag
       61 ttgcctggcc atccacgcta tatatacacg cctggcggat ctgcctggcg gatctgcctg
      121 gcggatctgc ctggcggatc tgcctggcgg atctgcctgg cggatctgct taagacacgt
      181 cctcatacac gtcctcatac acgtcctcat acacgtcctc atacacgtcc tcatacacgt
      241 cctcatacac gtcctcaaca cgtcctcacc taggcgtttg agaccgacta cggttatcca
      301 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
      361 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
      421 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
      481 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
      541 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
      601 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
      661 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
      721 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
      781 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
      841 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
      901 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
      961 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     1021 acgaaaactc acgttaaggg attttggtca tgattgatcg ggcacgtaag aggttccaac
     1081 tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca
     1141 ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc
     1201 caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac
     1261 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
     1321 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc ggaatttcgt
     1381 atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt
     1441 ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg
     1501 cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc
     1561 cctaaagggt ttattgagaa tatgtttttc gtttcagcca atccctgggt gagtttcacc
     1621 agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc
     1681 aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc
     1741 gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag
     1801 tggcagggcg gggcgtaatt tgatatcgag ctcgcttgga ctcctgttga tagatccagt
     1861 aatgacctca gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt
     1921 tattggtctg gtctcacata ttagtgtgtg tatttgtgtt tgtgtgtcta tagaagtata
     1981 gtaatttatg ctgcaaaggt cctaatgtat aaggaaagaa tatttagaga aaagaagaaa
     2041 acaagagttt tatatacata cagagcacat gcat
//
