LOCUS       sequence-381915-        2368 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Zif268 Op (Part 2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Sanford A, Kiriakov S, Khalil AS
  TITLE     A Toolkit for Precise, Multigene Control in Saccharomyces 
            cerevisiae.
  JOURNAL   ACS Synth Biol. 2022 Nov 11. doi: 10.1021/acssynbio.2c00423.
  PUBMED    36367334
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2022 Nov 11. doi: 10.1021/acssynbio.2c00423."
COMMENT     Sequence Label: pAS168
FEATURES             Location/Qualifiers
     source          1..2368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(94..682)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(174..193)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             864..1523
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(930..949)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1594..1628
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     primer_bind     complement(1663..1686)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
       61 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
      121 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
      181 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
      241 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
      301 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
      361 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
      421 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
      481 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
      541 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
      601 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
      661 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
      721 gtggaacgaa aactcacgtt aagggatttt ggtcatgatt gatcgggcac gtaagaggtt
      781 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
      841 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
      901 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
      961 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
     1021 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
     1081 ttcgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
     1141 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
     1201 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
     1261 atttccctaa agggtttatt gagaatatgt ttttcgtttc agccaatccc tgggtgagtt
     1321 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
     1381 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
     1441 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
     1501 atgagtggca gggcggggcg taatttgata tcgagctcgc ttggactcct gttgatagat
     1561 ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg ccgccgggcg
     1621 ttttttattg gtctggtctc acatatatag ttttttctcc ttgacgttaa agtatagagg
     1681 tatattaaca attttttgtt gatactttta tgacatttga ataagaagta atacaaactg
     1741 aaaatgttga aagtattagt taaagtggtt atgcagcttt tccatttata tatctgttaa
     1801 tagatcaaaa atcatcgctt cgctgattaa ttaccccaga aataaggcta aaaaactaat
     1861 cgcattatca tcctatggtt gttaatttga ttcgttaatt tgaaggtttg tggggccagg
     1921 ttactgccaa tttttcctct tcataaccat aaaagctagt attgtagaat ctttattgtt
     1981 cggagcagtg cggcattagg cacatctgcg tttcaggaac gcgaccggtg aagacgagga
     2041 cgcacggtct agacgcccac gcacgcccac gcccgcccac gcacgcccac gcccgcccac
     2101 gcacgcccac cttaagactt caatatagca atgagcagtt aagcgtatta ctgaaagttc
     2161 caaagagaag gtttttttag gctaagataa tggggctctt tacatttcca caacatataa
     2221 gtaagattag atatggatat gtatatggat atgtatatgg tggtaatgcc atgtaatatg
     2281 attattaaac ttctttgcgt ccatccaaaa aaaaataaga atttttgaaa attcaatata
     2341 acctaggcgt ttgagaccga ctacggtt
//
