LOCUS       sequence-381940-        9316 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation, Inducible lentiviral expression, TRE-gateway; 
            PGK-rtTA-2A-blasti.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9316)
  TITLE     Superti-Furga RESOLUTE modified pLIX plasmids
REFERENCE   2  (bases 1 to 9316)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCW57.1_TStrp_HA_Ct
FEATURES             Location/Qualifiers
     source          1..9316
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    51..168
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        222..536
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    230..248
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    266..284
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    301..319
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    337..355
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    373..391
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    408..426
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    444..462
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     509..533
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    557..681
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(706..711,712..729,730..736)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             790..1449
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(856..875)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1791..2096
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1975..1994
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2137..2261)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2308..2331
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2368..2391
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2422..2448
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2422..2448
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        2469..2979
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2898..2916
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2989..3384
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             3385..3438
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3439..4056,4057..4062,4063..4098,4099..4101,4102..4137,
                     4138..4140,4141..4176,4177..4185)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4225..4245)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4643..4654)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4859..5092
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5164..5285
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5201..5220)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5255..5274
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5325..5460
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5460..5531
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5532..5600,5601..6392)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5750..5769)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6563..7151
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7052..7071
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7305..7322
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7439..7460
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7475..7480,7481..7498,7499..7505)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7513..7529
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7518..7540
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7537..7553
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7537..7553
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7572..7592
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7574..7592
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7620..7846
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7847..8027
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8074..8199
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8692..8925
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9110..9154
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ccgaggggac aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga
       61 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
      121 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
      181 agggacagca gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt
      241 gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac
      301 tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta
      361 tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga
      421 tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc
      481 gtgtacggtg ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga
      541 gaattggcta gcatcaacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat
      601 aaatatcaat atattaaatt agattttgca taaaaaacag actacataat actgtaaaac
      661 acaacatatc cagtcactat ggcggccgca ttaggcaccc caggctttac actttatgct
      721 tccggctcgt ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag
      781 gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat
      841 cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt
      901 cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg
      961 gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg
     1021 aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag
     1081 caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta
     1141 cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg
     1201 tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat
     1261 ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat
     1321 acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat
     1381 ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc
     1441 ggggcgtaaa gatctggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc
     1501 gctgattttt gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga
     1561 ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca
     1621 aggcatatat gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg
     1681 tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg
     1741 gtttattgaa atgaacggct cttttgctga cgagaacagg ggctggtgaa atgcagttta
     1801 aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata
     1861 ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag
     1921 ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga
     1981 tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca
     2041 gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt
     2101 caggctccct tatacacagc cagtctgcag gtcgaccata gtgactggat atgttgtgtt
     2161 ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata
     2221 tcattttacg tttctcgttc agctttcttg tacaaagtgg ttgatatcaa ggccgccgac
     2281 atcaccagcc tgtacaagaa agtggggtgg agccatcccc agttcgagaa gggcggagga
     2341 tctggcggcg gaagcggcgg aggaagctgg tcccaccctc agtttgagaa aggcaccgag
     2401 ctgggcagca ccatggccag ctacccctac gacgtgcccg actacgcctg atgactcgag
     2461 tcggatccgg ggttggggtt gcgccttttc caaggcagcc ctgggtttgc gcagggacgc
     2521 ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg accctgggtc tcgcacattc
     2581 ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg ccccccggcg
     2641 acgcttcctg ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg
     2701 tgacaaacgg aagccgcacg tctcactagt accctcgcag acggacagcg ccagggagca
     2761 atggcagcgc gccgaccgcg atgggctgtg gccaatagcg gctgctcagc agggcgcgcc
     2821 gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc
     2881 ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc
     2941 ggctccctcg ttgaccgaat caccgacctc tctccccagc aattcaccat ggccaagcct
     3001 ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc
     3061 atctctgaag actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact
     3121 ggtgtcaatg tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact
     3181 gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg
     3241 ggcatcttga gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc
     3301 aaagccatag tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg
     3361 ccctctggtt atgtgtggga gggcgaaggt agaggttctc tcctcacttg tggtgatgtt
     3421 gaagaaaacc ctggtccaat gtctagactg gacaagagca aagtcataaa cggagctctg
     3481 gaattactca atggtgtcgg tatcgaaggc ctgacgacaa ggaaactcgc tcaaaagctg
     3541 ggagttgagc agcctaccct gtactggcac gtgaagaaca agcgggccct gctcgatgcc
     3601 ctgccaatcg agatgctgga caggcatcat acccacttct gccccctgga aggcgagtca
     3661 tggcaagact ttctgcggaa caacgccaag tcataccgct gtgctctcct ctcacatcgc
     3721 gacggggcta aagtgcatct cggcacccgc ccaacagaga aacagtacga aaccctggaa
     3781 aatcagctcg cgttcctgtg tcagcaaggc ttctccctgg agaacgcact gtacgctctg
     3841 tccgccgtgg gccactttac actgggctgc gtattggagg aacaggagca tcaagtagca
     3901 aaagaggaaa gagagacacc taccaccgat tctatgcccc cacttctgag acaagcaatt
     3961 gagctgttcg accggcaggg agccgaacct gccttccttt tcggcctgga actaatcata
     4021 tgtggcctgg agaaacagct aaagtgcgaa agcggcgggc cgaccgacgc ccttgacgat
     4081 tttgacttag acatgctccc agccgatgcc cttgacgact ttgaccttga tatgctgcct
     4141 gctgacgctc ttgacgattt tgaccttgac atgctccccg ggtaactaag taaggatcga
     4201 tccaagatat cgtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     4261 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     4321 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     4381 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     4441 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     4501 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     4561 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     4621 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     4681 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     4741 cctttgggcc gcctccccgc ctgtttcgcc tcggcgtccg gactagaggt acctttaaga
     4801 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     4861 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     4921 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     4981 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     5041 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     5101 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     5161 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     5221 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     5281 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
     5341 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     5401 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     5461 tttcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     5521 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     5581 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     5641 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     5701 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     5761 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     5821 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     5881 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     5941 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     6001 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     6061 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     6121 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     6181 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     6241 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     6301 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     6361 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     6421 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     6481 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     6541 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6601 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6661 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     6721 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6781 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6841 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6901 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6961 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     7021 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     7081 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     7141 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     7201 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     7261 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     7321 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     7381 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     7441 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     7501 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     7561 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     7621 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     7681 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     7741 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     7801 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt
     7861 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     7921 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     7981 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     8041 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     8101 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     8161 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     8221 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     8281 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     8341 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     8401 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     8461 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     8521 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     8581 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     8641 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     8701 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     8761 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     8821 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     8881 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     8941 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     9001 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     9061 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     9121 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     9181 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     9241 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     9301 gacccacctc ccaacc
//
