LOCUS       sequence-381956-        8531 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMV overexpression of ChemoL-NAD in the mitochondria of mammalian 
            cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8531)
  AUTHORS   Hellweg L, Edenhofer A, Barck L, Huppertz MC, Frei MS, Tarnawski M, 
            Bergner A, Koch B, Johnsson K, Hiblot J
  TITLE     A general method for the development of multicolor biosensors with 
            large dynamic ranges.
  JOURNAL   Nat Chem Biol. 2023 Jun 8. doi: 10.1038/s41589-023-01350-1.
  PUBMED    37291200
REFERENCE   2  (bases 1 to 8531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2023 Jun 8. doi: 10.1038/s41589-023-01350-1."
COMMENT     Sequence Label: pCDNA5/FRT/TO-[4xCox8]-ChemoL-NAD
FEATURES             Location/Qualifiers
     source          1..8531
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             984..1058
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             1071..1142
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="SVLTPLLLRGLTGSARRLPVPRAK"
     CDS             1161..1232
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="SVLTPLLLRGLTGSARRLPVPRAK"
     CDS             1245..1316
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="SVLTPLLLRGLTGSARRLPVPRAK"
     CDS             join(1896..1898,1899..2411)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIE"
     primer_bind     complement(1938..1959)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2199..2218)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2546..2567
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4483..4500)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4489..4713
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4846..4865)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4997..5044
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5052..6074
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5052..6074
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6204..6325
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6241..6260)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6295..6314
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6374..6390)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6374..6390)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6387..6409)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6398..6414
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6422..6428,6429..6446,6447..6452))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6467..6488
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6605..6622)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6776..7364)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6856..6875)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7535..8326,8327..8395))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8158..8177
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8396..8500)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttacc accatgtccg tcctgacgcc gctgctgctg cggggcttga
     1021 caggctcggc ccggcggctc ccagtgccgc gcgccaagat ccattcgttg tccgtcctga
     1081 cgccgctgct gctgcggggc ttgacaggct cggcccggcg gctcccagtg ccgcgcgcca
     1141 agatccattc gttgggaggc agcgtgctca ccccactcct gctgcggggc ttgacaggct
     1201 cggcccggcg gctcccagtg ccgcgcgcta aaattcacag cctctccgtc ctgacgccgc
     1261 tgctgctgcg gggcttgaca ggctcggccc ggcggctccc agtgccgcgc gccaagatcc
     1321 attcgttggg aggcagcgga ggcagcggtc tgagcggcga ccaaatgggc cagatcgaaa
     1381 aaatttttaa ggtggtgtac cctgtggatg atcatcactt taaggtgatc ctgcactatg
     1441 gcacactggt aatcgacggg gttacgccga acatgatcga ctatttcgga cggccgtatg
     1501 aaggcatcgc cgtgttcgac ggcaaaaaga tcactgtaac agggaccctg tggaacggca
     1561 acaaaattat cgacgagcgc ctgatcaacc ccgacggctc cctgctgttc cgagtaacca
     1621 tcaacggagt gaccggctgg cggctgtgcg aacgcattct ggcgggtggc accggtggct
     1681 ctggcggtac cggtggctct atggtcttca cactcgaaga tttcgttggg gactggcgac
     1741 agacagccgg ctacaacctg gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc
     1801 agaatctcgg ggtgtccgta actccgatcc aaaggattgt cctgagcggt gaaaatgggc
     1861 tgaagatcga catccatgtc atcatcccgt atgaagtgag caagggcgag gagctgttca
     1921 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     1981 tatccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     2041 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     2101 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     2161 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     2221 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     2281 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     2341 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     2401 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     2461 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc aaactgagca
     2521 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgcgc gccgccggga
     2581 tcactggtgg caccatgaca ctggaagaag ctcgtaaacg tgtaaacgaa cttcgtgatc
     2641 tgatccgtta tcacaattac cggtactacg tgttggccga ccccgagatt agcgatgcag
     2701 agtacgatcg tctgctgcgc gaactgaagg agttggagga acgttttcct gagctcaaat
     2761 cgccggattc accgacatta caagtgggcg cacggccgct ggaagccacg ttccgtcccg
     2821 tgcgccatcc gacacgcatg tacagtctgg ataacgcatt caacttggat gaactgaaag
     2881 cctttgagga acgtatcgaa cgcgcgctcg ggcgcaaagg tccgttcgcc tatacagttg
     2941 aacatctagt ggatggcctg agcgtcaact tatattacga agaaggagtg ttggtttacg
     3001 gcgcaacgcg tggcgacggt gaagtgggtg aggaagttac tcaaaattta ctcaccatcc
     3061 cgactattcc acgccgtctg aaaggtgtcc cggaacgcct cgaggtgcgt ggcgaagtgt
     3121 acatgccgat tgaggcgttt ctgcgcctga atgaagaact ggaggaacgt ggcgagcgca
     3181 tttttaaaaa tccgcgtaac gctgcagcag gcagcttgcg ccagaaagat ccgcgcatta
     3241 cggctaagcg tggtttgcgc gcaaccttct gggcgttagg tctgggtctc gaagaggtgg
     3301 agcgtgaagg agtagcgact caatttgcgt tgctgcattg gctcaaagag aaagggttcc
     3361 cggtcgaaca cggatatgca cgtgccgttg gggcggaagg cgtggaggcg gtttatcagg
     3421 actggctgaa aaaacgccgt gcgctgccgt ttgaggcgaa tggagttgcg gtcaaactgg
     3481 atgaacttgc cctgtggcgt gaactgggat acaccgcgcg tgcaccgcgt tttgccattg
     3541 cgtacaagtt cccttctggc ggtatcggta ctggctttcc attcgacccc cattatgtgg
     3601 aagtcctggg cgagcgcatg cactacgtcg atgttggtcc gcgcgatggc acccctgtgc
     3661 tgttcctgca cggtaacccg acctcctcct acgtgtggcg caacatcatc ccgcatgttg
     3721 caccgaccca tcgctgcatt gctccagacc tgatcggtat gggcaaatcc gacaaaccag
     3781 acctgggtta tttcttcgac gaccacgtcc gcttcatgga tgccttcatc gaagccctgg
     3841 gtctggaaga ggtcgtcctg gtcattcacg actggggctc cgctctgggt ttccactggg
     3901 ccaagcgcaa tccagagcgc gtcaaaggta ttgcatttat ggagttcatc cgccctatcc
     3961 cgacctggga cgaatggcca gaatttgccc gcgagacctt ccaggccttc cgcaccaccg
     4021 acgtcggccg caagctgatc atcgatcaga acgtttttat cgagggtacg ctgccgatgg
     4081 gtgtcgtccg cccgctgact gaagtcgaga tggaccatta ccgcgagccg ttcctgaatc
     4141 ctgttgaccg cgagccactg tggcgcttcc caaacgagct gccaatcgcc ggtgagccag
     4201 cgaacatcgt cgcgctggtc gaagaataca tggactggct gcaccagtcc cctgtcccga
     4261 agctgctgtt ctggggcacc ccaggcgttc tgatcccacc ggccgaagcc gctcgcctgg
     4321 ccaaaagcct gcctaactgc aaggctgtgg acatcggccc gggtgaaaat ctgctgcaag
     4381 aagacaaccc ggacctgatc ggcagcgaga tcgcgcgctg gctgtctact ctggagattt
     4441 ccggttagta atctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
     4501 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     4561 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     4621 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     4681 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     4741 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     4801 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     4861 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     4921 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     4981 atggttcacg tacctagaag ttcctattcc gaagttccta ttctctagaa agtataggaa
     5041 cttccttggc caaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa
     5101 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca
     5161 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct
     5221 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc
     5281 ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg
     5341 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg
     5401 ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac
     5461 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc
     5521 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc
     5581 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg
     5641 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga
     5701 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt
     5761 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag
     5821 gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct
     5881 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc
     5941 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga
     6001 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga
     6061 gggcaaagga atagcacgta ctacgagatt tcgattccac cgccgccttc tatgaaaggt
     6121 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     6181 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     6241 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     6301 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     6361 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     6421 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     6481 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     6541 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     6601 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     6661 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     6721 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     6781 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     6841 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     6901 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     6961 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     7021 tgggctgtgt acacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     7081 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     7141 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     7201 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     7261 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     7321 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     7381 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     7441 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     7501 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     7561 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     7621 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     7681 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     7741 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     7801 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     7861 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     7921 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     7981 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     8041 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     8101 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     8161 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     8221 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     8281 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     8341 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     8401 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     8461 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     8521 cacctgacgt c
//
