LOCUS       sequence-38196-e         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 3186 (=HL 716 + pHL 282)
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    135..151
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        209..313
                     /gene="bla"
                     /label=AmpR promoter
     CDS             498..876
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(532..551)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 atgctagctg ggaccaggag ggacagtact ttaactttca tgttattaac cctctgttat
       61 atgcctttat ttgctttttt atgccactgc atactgatta accctcatta atcagtcggc
      121 aagtcgacag tatcttgtta tccgctcaca atgtcaatgt tatccgctca catttatgac
      181 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
      241 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
      301 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
      361 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
      421 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
      481 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
      541 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
      601 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
      661 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
      721 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
      781 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
      841 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
      901 acttactcta gc
//
