LOCUS       sequence-381960-        6474 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmAdh-5BoxB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6474)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 6474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-DmAdhV5-5BoxB_X
FEATURES             Location/Qualifiers
     source          1..6474
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        49..2558
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             3433..3474
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    3559..3577
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    3601..3619
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    3625..3643
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    3683..3701
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    3707..3725
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     CDS             3773..3814
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3824..3841
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3864..3881)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3914..4048
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3938..3957)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3992..4011
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(4649..5237)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4729..4748)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5408..6199,6200..6268))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6031..6050
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6269..6373)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6441..6459
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aattaattct atattctaaa aacacaaatg atacttctaa aaaaaaatca tgaatggcat
       61 caactctgaa tcaaatcttt gcagatgcac ctacttctca tttccactgt cacatcattt
      121 ttccagatct cgctgcctgt tatgtggccc acaaaccaag acacgtttta tggccattaa
      181 agctggctga tcgtcgccaa acaccaaata cataatgaat atgtacacat tcgagaaaga
      241 agcgatcaaa gaagcgtctt cgggcggagt aggagaatgc ggaggagaag gagaacgagc
      301 tgatctagta tctctccaca atccaatgcc aactgaccaa ctggccatat tcggagcaat
      361 ttgaagccaa tttccatcgc ctggcgatcg ctccattctt ggctatatgt ttttcaccgt
      421 tacccggggc cattttcaaa gactcgtcgg caagataaga ttgtgtcact cgctgtctct
      481 cttcatttgt cgaagaatgc tgaggaattt cgcgatgacg tcggcgagta ttttgaagaa
      541 tgagaataat ttgtatttat acgaaaatca gttagtggaa ttttctacaa aaacatgtta
      601 tctatagata attttgttgc aaaatatgtt gactatgaca aagattgtat gtatatacct
      661 ttaatgtatt ctcattttct tatgtattta taatggcaat gatgatactg atgatatttt
      721 aagatgatgc cagaccaaaa ggcttgaatt tctgcgtctt ttgccgaacg cagtgcatgt
      781 gcaattgttg ttttttggaa tattcaattt tcggactgtc cgctttgatt tcagtttctt
      841 ggcttattca aaaagcaaag taaagccaaa aaagcgagat ggcaatacca aatgcggcaa
      901 aacggtagtg gaaggaaagg ggtgcggggc agcggaagga agggtggggc ggggcgtggc
      961 ggggtctgtg gctgggcgcg acgtcaccga cgttggagcc actcctttga ccatgtgtgc
     1021 gtgtgtgtat tattcgtgtc tcgccactcg ccggttgttt ttttcttttt atgctgcgct
     1081 ctctctagcg ccatctcgct tacgcatgct caacgcaccg catgttgccg tttcctttta
     1141 tgcgtcattt tggctcgaaa taggcaatta tttaaacaaa gattagtcaa cgaaaacgct
     1201 aaaataaata agtctacaat atggttactt attgccatgt gtgtgcagcc aacgatagca
     1261 acaaaagcaa caacacaggt ggctttccct ctttcacttt ttgtttgcaa gccgcgtgcg
     1321 agcaagacgg cacgaccggc aaacgcaatt acgctgacaa agagcagacg aagttttggc
     1381 gaaaaacatc aaggcgcctg atacgaatgc atttgcaata acaattgcga tatttaatat
     1441 tgtttatgaa gctgtttgac ttcaaaacac acaaaaaaaa aaataaaaca aattatttga
     1501 aagagaatta ggaatcggac gcttatcgtt agggtaacaa caagaaatgc ttactgagtc
     1561 acagcctctg gaaaactgcc gcaagccaga gagagagaga aaaagaggga gagcagctta
     1621 gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tctgttgcgc aaacgcatag
     1681 actgcactga aaaaatcgat tacctatttt ttatgaatga atatttgcac tattactatt
     1741 caaaactatt aagatagcaa tcacattcaa tagccaaata ctataccacc tgagcgatgc
     1801 aacgaaatga tcaatttgag caaaaatgct gcatatttag gacggcatca ttatagaaat
     1861 gcttcttgct gtgtactttt ctctcgtctg gcagctgttt cgccgttatt gttaaaaccg
     1921 gcttaagtta ggtgtgtttt ctacgactag tgaatgccct actagaagat gtgtgttgca
     1981 caaaatgtcc ctggaataac caatttgaag tgcagatagc agtaaacgta agctaatatg
     2041 aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac ccactataaa
     2101 cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa tgtgtaaaag
     2161 caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga gtaaccccca
     2221 aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca tggcgatata
     2281 caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa atgaaaaccc
     2341 aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact tgagagcgga
     2401 gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg gctgcgggac
     2461 cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct cccgacacaa
     2521 agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg tacctactag
     2581 tccagtgtgg tggaattcca acatgtcgtt tactttgacc aacaagaacg tgattttcgt
     2641 tgccggtctg ggaggcattg gtctggacac cagcaaggag ctgctcaagc gcgatctgaa
     2701 ggtaactatg cgatgcccac aggctccatg cagcgatgga ggttaatctc gtgtattcaa
     2761 tcctagaacc tggtgatcct cgaccgcatt gagaacccgg ctgccattgc cgagctgaag
     2821 gcaatcaatc caaaggtgac cgtcaccttc tacccctatg atgtgaccgt gcccattgcc
     2881 gagaccacca agctgctgaa gaccatcttc gcccagctga agaccgtcga tgtcctgatc
     2941 aacggagctg gtatcctgga cgatcaccag atcgagcgca ccattgccgt caactacact
     3001 ggcctggtca acaccacgac ggccattctg gacttctggg acaagcgcaa gggcggtccc
     3061 ggtggtatca tctgcaacat tggatccgtc actggattca atgccatcta ccaggtgccc
     3121 gtctactccg gcaccaaggc cgccgtggtc aacttcacca gctccctggc gaaactggcc
     3181 cccattaccg gcgtgaccgc ttacaccgtg aaccccggca tcacccgcac caccctggtg
     3241 cacaagttca actcctggtt ggatgttgag ccccaggttg ctgagaagct cctggctcat
     3301 cccacccagc catcgttggc ctgcgccgag aacttcgtca aggctatcga actgaaccag
     3361 aacggagcca tctggaaact ggacttgggc accctggagg ccatccagtg gaccaagcac
     3421 tgggactccg gcggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtaaatc
     3481 tcgagggaat tcgctagctt tcccgctcga gggaattcgc tagcttccct aagtccaact
     3541 accaaactgg ggattcctgg gccctgaaga agggcccctc gactagtcca aatacaaact
     3601 gggccctgaa gaagggccca tatagggccc tgaagaaggg ccctatcgag gatattatct
     3661 cgactaagtc caactacaaa ctgggccctg aagaagggcc catatagggc cctgaagaag
     3721 ggccctatcg aggatattat ctcgaagtca cccattcgaa ccgcggttcg aaggtaagcc
     3781 tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc accatcacca
     3841 ttgagtttaa acccgctgat cagcctcgac tgtgccttct aaggcctgag ctcgctgatc
     3901 agcctcgatc gaggatccag acatgataag atacattgat gagtttggac aaaccacaac
     3961 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     4021 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     4081 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat
     4141 ggctgattat gatcagtcga ccgatgccct tgagagcctt caacccagtc agctccttcc
     4201 ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac
     4261 tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc tttcgctgga
     4321 gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag
     4381 ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt atcgccggca
     4441 tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct
     4501 tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc
     4561 tgtccaggca ggtagatgac gaccatcagg gacagcttca aggccagcaa aaggccagga
     4621 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     4681 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     4741 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     4801 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     4861 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     4921 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     4981 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     5041 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     5101 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     5161 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     5221 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     5281 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     5341 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     5401 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     5461 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     5521 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     5581 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     5641 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     5701 tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg
     5761 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     5821 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     5881 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     5941 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     6001 cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa
     6061 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     6121 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     6181 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     6241 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     6301 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     6361 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     6421 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt caag
//
