LOCUS       sequence-381965-        9527 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmGe1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9527)
  AUTHORS   Behm-Ansmant I, Rehwinkel J, Doerks T, Stark A, Bork P, Izaurralde E
  TITLE     mRNA degradation by miRNAs and GW182 requires both CCR4:NOT 
            deadenylase and DCP1:DCP2 decapping complexes.
  JOURNAL   Genes Dev. 2006 Jul 15. 20(14):1885-98.
  PUBMED    16815998
REFERENCE   2  (bases 1 to 9527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2006 Jul 15. 20(14):1885-98."
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmGe1_J
FEATURES             Location/Qualifiers
     source          1..9527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     15..33
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        97..2606
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2626..2691
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2716..2742
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      4601..4610
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6874..6915
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6925..6942
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6965..6982)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7015..7149
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7039..7058)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7093..7112
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7750..8338)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7830..7849)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8509..9300,9301..9369))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9132..9151
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9370..9474)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa ttaattctat
       61 attctaaaaa cacaaatgat acttctaaaa aaaaatcatg aatggcatca actctgaatc
      121 aaatctttgc agatgcacct acttctcatt tccactgtca catcattttt ccagatctcg
      181 ctgcctgtta tgtggcccac aaaccaagac acgttttatg gccattaaag ctggctgatc
      241 gtcgccaaac accaaataca taatgaatat gtacacattc gagaaagaag cgatcaaaga
      301 agcgtcttcg ggcggagtag gagaatgcgg aggagaagga gaacgagctg atctagtatc
      361 tctccacaat ccaatgccaa ctgaccaact ggccatattc ggagcaattt gaagccaatt
      421 tccatcgcct ggcgatcgct ccattcttgg ctatatgttt ttcaccgtta cccggggcca
      481 ttttcaaaga ctcgtcggca agataagatt gtgtcactcg ctgtctctct tcatttgtcg
      541 aagaatgctg aggaatttcg cgatgacgtc ggcgagtatt ttgaagaatg agaataattt
      601 gtatttatac gaaaatcagt tagtggaatt ttctacaaaa acatgttatc tatagataat
      661 tttgttgcaa aatatgttga ctatgacaaa gattgtatgt atataccttt aatgtattct
      721 cattttctta tgtatttata atggcaatga tgatactgat gatattttaa gatgatgcca
      781 gaccaaaagg cttgaatttc tgcgtctttt gccgaacgca gtgcatgtgc aattgttgtt
      841 ttttggaata ttcaattttc ggactgtccg ctttgatttc agtttcttgg cttattcaaa
      901 aagcaaagta aagccaaaaa agcgagatgg caataccaaa tgcggcaaaa cggtagtgga
      961 aggaaagggg tgcggggcag cggaaggaag ggtggggcgg ggcgtggcgg ggtctgtggc
     1021 tgggcgcgac gtcaccgacg ttggagccac tcctttgacc atgtgtgcgt gtgtgtatta
     1081 ttcgtgtctc gccactcgcc ggttgttttt ttctttttat gctgcgctct ctctagcgcc
     1141 atctcgctta cgcatgctca acgcaccgca tgttgccgtt tccttttatg cgtcattttg
     1201 gctcgaaata ggcaattatt taaacaaaga ttagtcaacg aaaacgctaa aataaataag
     1261 tctacaatat ggttacttat tgccatgtgt gtgcagccaa cgatagcaac aaaagcaaca
     1321 acacaggtgg ctttccctct ttcacttttt gtttgcaagc cgcgtgcgag caagacggca
     1381 cgaccggcaa acgcaattac gctgacaaag agcagacgaa gttttggcga aaaacatcaa
     1441 ggcgcctgat acgaatgcat ttgcaataac aattgcgata tttaatattg tttatgaagc
     1501 tgtttgactt caaaacacac aaaaaaaaaa ataaaacaaa ttatttgaaa gagaattagg
     1561 aatcggacgc ttatcgttag ggtaacaaca agaaatgctt actgagtcac agcctctgga
     1621 aaactgccgc aagccagaga gagagagaaa aagagggaga gcagcttaga ccgcatgtgc
     1681 ttgtgtgtga ggcgtctctc tcttcgtctc tgttgcgcaa acgcatagac tgcactgaaa
     1741 aaatcgatta cctatttttt atgaatgaat atttgcacta ttactattca aaactattaa
     1801 gatagcaatc acattcaata gccaaatact ataccacctg agcgatgcaa cgaaatgatc
     1861 aatttgagca aaaatgctgc atatttagga cggcatcatt atagaaatgc ttcttgctgt
     1921 gtacttttct ctcgtctggc agctgtttcg ccgttattgt taaaaccggc ttaagttagg
     1981 tgtgttttct acgactagtg aatgccctac tagaagatgt gtgttgcaca aaatgtccct
     2041 ggaataacca atttgaagtg cagatagcag taaacgtaag ctaatatgaa tattatttaa
     2101 ctgtaatgtt ttaatatcgc tggacattac taataaaccc actataaaca catgtacata
     2161 tgtatgtttt ggcatacaat gagtagttgg ggaaaaaatg tgtaaaagca ccgtgaccat
     2221 cacagcataa agataaccag ctgaagtatc gaatatgagt aacccccaaa ttgaatcaca
     2281 tgccgcaact gataggaccc atggaagtac actcttcatg gcgatataca agacacacac
     2341 aagcacgaac acccagttgc ggaggaaatt ctccgtaaat gaaaacccaa tcggcgaaca
     2401 attcataccc atatatggta aaagttttga acgcgacttg agagcggaga gcattgcggc
     2461 tgataaggtt ttagcgctaa gcgggcttta taaaacgggc tgcgggacca gttttcatat
     2521 cactaccgtt tgagttcttg tgctgtgtgg atactcctcc cgacacaaag ccgctccatc
     2581 agccagcagt cgtctaatcc agagaccccg gatcggggta ccaacatgga cgcacaaaca
     2641 cgacgacgtg agcgtcgcgc tgagaaacaa gctcaatgga aagctgcaaa cggtggatct
     2701 cctccacata tggcttaccc atacgatgtt ccagattacg ctcctccaag cttgaattct
     2761 gcagatatcc agcacagtgg cggccgcatg ttaatcgcgc tcttcgcgct tgcacacttg
     2821 ccaatctttc gtaagacgcc tttgtcagcc tcctcctccc cgccgccgtc tgtccaccgg
     2881 tcgccaaggt gtggcaaagc aagtaccgcg taccatccac cgccagcaga cgcctgcatc
     2941 agcattactg cttcagccat tgacgcagca tccgggatga gcaacagcgc cgaacaggac
     3001 aagccgattg ccgccaacgg caatgcggca gagccccagg aaatagaagt catccatttc
     3061 caagcgcaag agaagcagtg ttgccgcgtt attagatcca ataagaccaa ggtcctcaca
     3121 tccggcggcg ttcacacacg cggttcctcc aaggtcaagc taaagaacat cgtcgactac
     3181 aagtgggagc gcaagtacta ctatccgggt cacctggtcg ctgtccaccg cgatggcaag
     3241 cacctggcgt acgccattaa tgtcaacaac aaagccaccg gaatggaggg catggtgcgc
     3301 gtatgcaaca tagccaccag tatgcgagcc ttgatcaagg gaatgagcgg cgaggtctta
     3361 gatctgcggt ttgcccatac ggattgtgag cgtatattag ctgtgatcga tgtcagttcg
     3421 ctgtttgtct acaaagtcga tcaaatcgaa gggaacctgc tgtgcaatct agtgctaaag
     3481 gtggaggacc ccatcgccaa ctatgtgccg gagtacgata tggtctcctg gtgtccctat
     3541 gtctgcagca gcagcgccac cgttccaatc aacgacgatg acgatgagaa tcagctgctc
     3601 atttggtcgc gaagctctca atttcaatgc tttcaagtga agatgattgt ctctgaacat
     3661 gggcgcggca agatccaacc agcagcccta gaatctggat atctgaagat tgaggaggac
     3721 tcgttgatca cgtgcgccgc tttgtccccg gatggcacca ctgtggctgc tgcttgcgcc
     3781 gatggcttgg ttcgtttcta ccaaatttat ttgttcgatg tgcgcaacca tcgctgcctg
     3841 cacgagtgga agccacatga tggcaaaaaa gtctgctctc tcttttttct cgacaatatc
     3901 aacaagcctg tggaagaatc ctactggcag cacgtgatca ctacgagcga tgccaatacc
     3961 gaaatcaagc tctggaactg ctcgctatgg aaatgcctcc aaactatcaa tgtggtggcc
     4021 agtccgtcat ctttgcagcc cagaaacttt atcgcgggca tagatcgcag tgcgaactac
     4081 ttggtgttgt cttgcctgga ctctctggcc gtgtatgtaa tgcagattgg cagtactggc
     4141 ggcgcagact ccgagaatag gagtagcgat agcgaaggag aaggctgcga cacttccaaa
     4201 cgcattcaga atgttgccga attcaagctg agttcgggga ttctctcttt ttctattgta
     4261 aacgctagta tgcgtcgcgt taagaactcc atcgagagct actatcctat tgaggagccc
     4321 gatgacttcg atgacgattc caattccacc agtgcccttg tgctgcacat gtttgtggtg
     4381 caggcaaaga gcctgcagga atgtcaaata atctaccagc catgtgtggc tgaaaagacg
     4441 gagcggagca gcctaaacag caagcgatca cagacgccag aagacaatct gctaatcaaa
     4501 gaggagccgg aatcccccaa cagtggaact gtgggtgctg ttcagctgga tgcattattc
     4561 gcaaaatccg caaagagagc ttcaacgggc agttcatctg ccatggtggc cgtggcggca
     4621 gccgcggctg cggcaccttc ggccatcctg caggacgcca ctaaggaggc ggccaagtca
     4681 gagtccccgc aactaagcag cgcgtatacg cagcaggtta atctcatgac tccagatgcc
     4741 ttcagtgcaa gcggaaccgc agcggcagca gctgtatttg tgtctaccag caccacaact
     4801 tcaataggaa ctgatagctc taccaccacc agcggtcagg atagatccat tgattcagcg
     4861 gttctgcaaa ctatacgaat gctggccacg gtaacgtcca agacttccga aaaccccaat
     4921 gccgaggtac tcctcaacct aatgaacaac accctaatcg aagatcgcga gcaacaaaag
     4981 ctgaaggaaa agcttgacgc tcgcaagaag ttcattgcaa ttgatcgcaa tccagagcgg
     5041 aatgtggctg agaacttggc cagcggcagt tcgagtccga gtcgagaggt gcaggagata
     5101 atggccacac aggacgatgc cgatgcctac gaagcagagc tagagaatct ggacgacgat
     5161 gatgacgatg aggaagaaga gctggcaaat tcatcaccgc tcccggaagc tgttgacgga
     5221 acatggccca ttgtcaagct gtcatcccac tcagctgagc tccagaatgc tgcccagatc
     5281 atgtcccagg cggtgcagaa caccaacaat ggcaatgtgc cgcctacgct aggtggaggg
     5341 cacaataata acacaagtgt aggcagcaat agcaacaaca acacggctac aactcttagc
     5401 accagcaaca ccagcagcag caacaacgcg ggcggaacat gcgttgattc aagtggaact
     5461 ggagagctaa atgccaaaat ggaactactc atagatctag taaaagccca gtccaaacaa
     5521 ataaacaaat tggagaacga agtcaacaag ctacaaaagc agcaagaagc ggcggcggcc
     5581 ctgcatagca aacaagacac ttcattggag cctaaaaatc ttagtcagct agcatataaa
     5641 attgagatgc aactgtccaa gctaatggag cagtatctca agcgatatga aaacgagcat
     5701 aagaagaaac tgacggaatt cttggcggcg cgtgagagcc aaaaccgaga gctacgtgac
     5761 tccgtgctgc aggtactaaa tcagtatgtc atgaaccact ttacggacac tattggcaac
     5821 gttctcaaca tggaactgca gcgccaactg cttcctcgcg tgaatgccaa catggatcag
     5881 ctccaagccc aaatgcaagt ggagattgtg cagaagctga gtgtgtttga caaaacggtg
     5941 aaggagaata ttgcccaggt gtgcaagagc aagcaatttc tggatacttt tggcaagtcc
     6001 gtgttgattg gtgttcaaac cagcctacaa accgccttca ttgaatctat gagcagcacc
     6061 ttgattccag cttatgagaa gtcctcgcag aacatgttta aacaactgca tgacgctttc
     6121 agcgtgggca taaaagactt tatggtccag tttaacacct acctgcagca catgccacag
     6181 ccccaagccg ggagtggcaa tacggaggag ataaacaaca agttgtccat gcttaagcaa
     6241 ttggtagaga gcagcttgca caagcaccgc actgaactca cggacgccat gctcgagaca
     6301 caaagagaag tgaagagctt agagattttg ctggcgcgtc aggtacagga aacgatcaga
     6361 gcagagctac gcaagcacat ggaagcccag aacgtggcaa tgcgttcgca ggcagctacg
     6421 cctgcaccac cttacgacct cagggacagc attaagcagc ttttgatggc tggacaaatc
     6481 aacaaggcct tccatcaggc tctattggcc aacgacttgg gcctagtgga gttcactctc
     6541 cgacacacgg acagcaacca ggcattcgca ccagagggat gcagactgga gcaaaaggtt
     6601 ctactgtcgc tcattcagca aatatccgct gacatgacca atcacaatga gcttaagcag
     6661 agatatctca acgaggcact gttggccatc aatatggcag atcccattac ccgcgagcac
     6721 gctcctaaag tgctcaccga gctgtatcgc aattgtcagc agttcattaa gaacagcccc
     6781 aagaattcgc agttcagcaa cgtccgtctg ctgatgaagg caatcataac gtaccgcgat
     6841 cagctaaaat gactagaggg cccgcggttc gaaggtaagc ctatccctaa ccctctcctc
     6901 ggtctcgatt ctacgcgtac cggtcatcat caccatcacc attgagttta aacccgctga
     6961 tcagcctcga ctgtgccttc taaggcctga gctcgctgat cagcctcgat cgaggatcca
     7021 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     7081 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     7141 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     7201 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcagtcg
     7261 accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
     7321 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
     7381 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct
     7441 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc
     7501 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg
     7561 ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct
     7621 cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga
     7681 cgaccatcag ggacagcttc aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     7741 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     7801 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     7861 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     7921 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     7981 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     8041 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     8101 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     8161 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     8221 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     8281 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     8341 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     8401 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     8461 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     8521 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     8581 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     8641 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     8701 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     8761 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     8821 tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     8881 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     8941 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     9001 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     9061 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     9121 gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     9181 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     9241 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     9301 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     9361 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     9421 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     9481 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatga
//
