LOCUS       sequence-381967-       11599 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human CRLF2 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11599)
  AUTHORS   Hurtz C, Wertheim GB, Loftus JP, Blumenthal D, Lehman A, Li Y, 
            Bagashev A, Manning B, Cummins KD, Burkhardt JK, Perl AE, Carroll M,
            Tasian SK
  TITLE     Oncogene-independent BCR-like signaling adaptation confers drug 
            resistance in Ph-like ALL.
  JOURNAL   J Clin Invest. 2020 Jul 1;130(7):3637-3653. doi: 10.1172/JCI134424.
  PUBMED    32191635
REFERENCE   2  (bases 1 to 11599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "J Clin Invest"; date: "2020-07-1- 1"; volume: "130"; issue: "7"; 
            pages: "3637-3653"
COMMENT     Sequence Label: LentiCRISPRv2 Blast CRLF2
FEATURES             Location/Qualifiers
     source          1..11599
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(13..33)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        18..347
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      198..333
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     260..279
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        395..442
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             461..835
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    965..1086
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1002..1021)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1056..1075
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1135..1151)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1135..1151)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1148..1170)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1159..1175
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1183..1189,1190..1207,1208..1213))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1228..1249
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1366..1383)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1537..2125)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1617..1636)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2296..3087,3088..3156))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2919..2938
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3157..3261)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3336..3355)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3531..3910
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3912..4110
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4065..4085
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4128..4308
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4355..4480
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4973..5206
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5391..5435
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5733..5850
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5901..6149
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     5901..5921
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6072..6091
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        8031..8106
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        8168..8379
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             9512..9568
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             9569..9964
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9980..10568
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10033..10053)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10451..10462)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10640..10873
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10899..10916)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10905..11129
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(11175..11599,1..4)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11262..11281)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11472..11493
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
       61 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
      181 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
      241 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
      301 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
      361 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta
      421 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
      481 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
      541 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
      601 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
      661 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
      721 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
      781 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt
      841 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
      901 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
      961 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     1021 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     1081 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     1141 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     1201 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     1261 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     1321 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     1381 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     1441 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     1501 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     1561 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     1621 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     1681 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     1741 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     1801 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     1861 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     1921 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     1981 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     2041 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     2101 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     2161 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     2221 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     2281 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     2341 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     2401 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     2461 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     2521 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     2581 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     2641 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     2701 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     2761 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     2821 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     2881 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     2941 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     3001 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     3061 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     3121 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     3181 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     3241 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
     3301 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
     3361 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
     3421 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
     3481 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgcgcgtt gacattgatt
     3541 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     3601 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     3661 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     3721 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     3781 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     3841 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     3901 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     3961 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     4021 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     4081 gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt
     4141 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     4201 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     4261 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     4321 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     4381 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     4441 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     4501 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     4561 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     4621 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     4681 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     4741 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     4801 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     4861 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     4921 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     4981 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     5041 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     5101 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     5161 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     5221 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     5281 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     5341 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     5401 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     5461 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     5521 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     5581 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     5641 gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa
     5701 ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     5761 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     5821 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     5881 gtttggttaa ttaaggtacc gagggcctat ttcccatgat tccttcatat ttgcatatac
     5941 gatacaaggc tgttagagag ataattagaa ttaatttgac tgtaaacaca aagatattag
     6001 tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat
     6061 gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt
     6121 tatatatctt gtggaaagga cgaaacaccg gagacggttg taaatgagca cacaaaatac
     6181 acatgctaaa atattatatt ctatgacctt tataaaatca accaaaatct tctttttaat
     6241 aactttagta tcaataatta gaatttttat gttccttttt gcaaactttt aataaaaatg
     6301 agcaaaataa aaaaacgcta gttttagtaa ctcgcgttgt tttcttcacc tttaataata
     6361 gctactccac cacttgttcc taagcggtca gctcctgctt caatcatttt ttgagcatct
     6421 tcaaatgttc taactccacc agctgcttta actaaagcat tgtctttaac aactgacttc
     6481 attagtttaa catcttcaaa tgttgcacct gattttgaaa atcctgttga tgttttaaca
     6541 aattctaatc cagcttcaac agctatttca caagctttca tgatttcttc ttttgttaat
     6601 aaacaatttt ccataataca tttaacaaca tgtgatccag ctgctttttt tacagctttc
     6661 atgtcttcta aaactaattc ataatttttg tcttttaatg caccaatatt taataccata
     6721 tcaatttctg ttgcaccatc tttaattgct tcagaaactt cgaatgcttt tgtagctgtt
     6781 gtgcatgcac ctagaggaaa acctacaaca tttgttattc ctacatttgt gccttttaat
     6841 aattctttac aatagcttgt tcaatatgaa ttaacacaaa ctgttgcaaa atcaaattca
     6901 attgcttcat cacataattg tttaatttca gctttcgtag catcttgttt taataatgtg
     6961 tgatctatat atttgtttag tttcattttt tctcctatat attcattttt aattttaatt
     7021 ctttaataat ttcgtctact ttaactttag cgttttgaac agattcacca acacctataa
     7081 aataaatttt tagtttaggt tcagttccac ttgggcgaac agcaaatcat gacttatctt
     7141 ctaaataaaa ttttagtaag tcttgtcctg gcatattata cattccatcg atgtagtctt
     7201 caacattaac aactttaagt ccagcaattt gagttaaggg tgttgctctc aatgatttca
     7261 ttaatggttc aatttttaat ttcttttctt ctggtttaaa attcaagttt aaagtgaaag
     7321 tgtaatatgc acccatttct ttaaataaat cttctaaata gtctactaat gttttatttt
     7381 gttttttata aaatcaagca gcctctgcta ttaatataga agcttgtatt ccatctttat
     7441 ctctagctga gtcatcaatt acatatccat aactttcttc ataagcaaaa acaaaattta
     7501 atccgttatc ttcttcttta gcaatttctc tacccattca tttaaatcca gttaaagttt
     7561 ttacaatatt aactccatat ttttcatgag cgattctatc acccaaatca cttgttacaa
     7621 aacttgaata tagagccgga ttttttggaa tgctatttaa gcgttttaga tttgataatt
     7681 ttcaatcaat taaaattggt cctgtttgat ttccatctaa tcttacaaaa tgaccatcat
     7741 gttttattgc cattccaaat ctgtcagcat ctgggtcatt cataataata atatctgcat
     7801 catgtttaat accatattca agcggtattt ttcatgcagg atcaaattct ggatttggat
     7861 ttacaacatt tttaaatgtt tcatcttcaa atgcatgctc ttcaacctca ataacgttat
     7921 atcctgattc acgtaatatt tttggggtaa atttagttcc tgttccatta actgcgctaa
     7981 aaataatttt taaatctttt ttagcttctt gctctttttt gtacgtctct gttttagagc
     8041 tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
     8101 cggtgctttt ttgaattcgc tagctaggtc ttgaaaggag tgggaattgg ctccggtgcc
     8161 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
     8221 aattgatccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
     8281 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
     8341 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg accggtgcca tggggcggct
     8401 ggttctgctg tggggagctg ccgtctttct gctgggaggc tggatggctt tggggcaagg
     8461 aggagcagca gaaggagtac agattcagat catctacttc aatttagaaa ccgtgcaggt
     8521 gacatggaat gccagcaaat actccaggac caacctgact ttccactaca gattcaacgg
     8581 tgatgaggcc tatgaccagt gcaccaacta ccttctccag gaaggtcaca cttcggggtg
     8641 cctcctagac gcagagcagc gagacgacat tctctatttc tccatcagga atgggacgca
     8701 ccccgttttc accgcaagtc gctggatggt ttattacctg aaacccagtt ccccgaagca
     8761 cgtgagattt tcgtggcatc aggatgcagt gacggtgacg tgttctgacc tgtcctacgg
     8821 ggatctcctc tatgaggttc agtaccggag ccccttcgac accgagtggc agtccaaaca
     8881 ggaaaatacc tgcaacgtca ccatagaagg cttggatgcc gagaagtgtt actctttctg
     8941 ggtcagggtg aaggctatgg aggatgtata tgggccagac acatacccaa gcgactggtc
     9001 agaggtgaca tgctggcaga gaggcgagat tcgggatgcc tgtgcagaga caccaacgcc
     9061 tcccaaacca aagctgtcca aatttatttt aatttccagc ctggccatcc ttctgatggt
     9121 gtctctcctc cttctgtctt tatggaaatt atggagagtg aagaagtttc tcattcccag
     9181 cgtgccagac ccgaaatcca tcttccccgg gctctttgag atacaccaag ggaacttcca
     9241 ggagtggatc acagacaccc agaacgtggc ccacctccac aagatggcag gtgcagagca
     9301 agaaagtggc cccgaggagc ccctggtagt ccagttggcc aagactgaag ccgagtctcc
     9361 caggatgctg gacccacaga ccgaggagaa agaggcctct gggggatccc tccagcttcc
     9421 ccaccagccc ctccaaggcg gtgatgtggt cacaatcggg ggcttcacct ttgtgatgaa
     9481 tgaccgctcc tacgtggcgt tgggatccgg cgcaacaaac ttctctctgc tgaaacaagc
     9541 cggagatgtc gaagagaatc ctggaccggc caagcctttg tctcaagaag aatccaccct
     9601 cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact acagcgtcgc
     9661 cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat atcattttac
     9721 tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg cagctggcaa
     9781 cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc cctgcggacg
     9841 gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga aggacagtga
     9901 tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg tgtgggaggg
     9961 ctaaacgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
    10021 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
    10081 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
    10141 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
    10201 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
    10261 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
    10321 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
    10381 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
    10441 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
    10501 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
    10561 ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt
    10621 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
    10681 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
    10741 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
    10801 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
    10861 aaaatctcta gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
    10921 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
    10981 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
    11041 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
    11101 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
    11161 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
    11221 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
    11281 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
    11341 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
    11401 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
    11461 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
    11521 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
    11581 ttaacaaaaa tttaacgcg
//
