LOCUS       sequence-381988-       10625 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pBac-U6-crGFP-array-UTR-opie2-eGFP-3xp3-tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10625)
  AUTHORS   Dalla Benetta E, Lopez-Denman AJ, Li HH, Masri RA, Brogan DJ, Bui M,
            Yang T, Li M, Dunn M, Klein MJ, Jackson S, Catalan K, Blasdell KR, 
            Tng P, Antoshechkin I, Alphey LS, Paradkar PN, Akbari OS
  TITLE     Engineered Antiviral Sensor Targets Infected Mosquitoes.
  JOURNAL   CRISPR J. 2023 Dec;6(6):543-556. doi: 10.1089/crispr.2023.0056.
  PUBMED    38108518
REFERENCE   2  (bases 1 to 10625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/crispr.2023"; journalName: "CRISPR J"; date: "2023-12"; 
            volume: "6"; issue: "6"; pages: "543-556"
COMMENT     Sequence Label: OA-1085L
FEATURES             Location/Qualifiers
     source          1..10625
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(95..683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(175..194)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(854..1645,1646..1714))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1477..1496
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1715..1819)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1887..1905
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1943..1965)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2065..2084
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2278..2300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2292..2309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2293..2309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   3013..3075
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     misc_RNA        5083..5118
                     /label=RfxCas13d DR36
                     /note="36-base direct repeat for an RfxCas13d pre-gRNA 
                     (Konermann et al., 2018)"
     misc_RNA        5149..5184
                     /label=RfxCas13d DR36
                     /note="36-base direct repeat for an RfxCas13d pre-gRNA 
                     (Konermann et al., 2018)"
     misc_RNA        5215..5250
                     /label=RfxCas13d DR36
                     /note="36-base direct repeat for an RfxCas13d pre-gRNA 
                     (Konermann et al., 2018)"
     misc_RNA        5281..5316
                     /label=RfxCas13d DR36
                     /note="36-base direct repeat for an RfxCas13d pre-gRNA 
                     (Konermann et al., 2018)"
     misc_RNA        5347..5382
                     /label=RfxCas13d DR36
                     /note="36-base direct repeat for an RfxCas13d pre-gRNA 
                     (Konermann et al., 2018)"
     CDS             join(6540..6542,6543..6545,6546..7259)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTSKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6585..6606)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6846..6865)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7193..7214
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7294..7375
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      7756..7765
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(7895..7913)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8163..8182
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     8357..8380
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    9314..9435
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   10103..10137
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(10318..10334)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10318..10334)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10331..10353)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10342..10358
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10366..10372,10373..10390,10391..10396))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10411..10432
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10549..10566)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
       61 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      121 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      181 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      241 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
      301 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      361 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      421 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      481 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
      541 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      601 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      661 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
      721 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
      781 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
      841 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      901 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      961 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1021 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1081 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1141 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1201 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1261 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1321 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1381 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1441 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1501 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1561 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1621 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1681 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1741 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1801 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     1861 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     1921 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     1981 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     2041 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     2101 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     2161 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     2221 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     2281 agtcacgacg ttgtaaaacg acggccagtg ccaagctttg tttaaaaata taacaaaatt
     2341 gtgatcccac aaaatgaagt ggggcaaaat caaataatta atagtgtccg taaacttgtt
     2401 ggtcttcaac tttttgagga acacgttgga cggcaaatcc gtgactataa cacaagttga
     2461 tttaataatt ttagccaaca cgtcgggctg cgtgtttttt gccgacgcgt ctgtgtacac
     2521 gttgattaac tggtcgatta aactgttgaa ataatttaat ttttggttct tctttaaatc
     2581 tgtgatgaaa ttttttaaaa taactttaaa ttcttcattg gtaaaaaatg ccacgttttg
     2641 caacttgtga gggtctaata tgaggtcaaa ctcagtagga gttttatcca aaaaagaaaa
     2701 catgattacg tctgtacacg aacgcgtatt aacgcagagt gcaaagtata agagggttaa
     2761 aaaatatatt ttacgcacca tatacgcatc gggttgatat cgttaatatg gatcaatttg
     2821 aacagttgat taacgtgtct ctgctcaagt ctttgatcaa aacgcaaatc gacgaaaatg
     2881 tgtcggacaa tatcaagtcg atgagcgaaa aactaaaaag gctagaatac gacaatctca
     2941 cagacagcgt tgagatatac ggtattcacg acagcaggct gaataataaa aaaattagaa
     3001 actattattt aaccctagaa agataatcat attgtgacgt acgttaaaga taatcatgcg
     3061 taaaattgac gcatgtgttt tatcggtctg tatatcgagg tttatttatt aatttgaata
     3121 gatattaagt tttattatat ttacacttac atactaataa taaattcaac aaacaattta
     3181 tttatgttta tttatttatt aaaaaaaaac aaaaactcaa aatttcttct ataaagtaac
     3241 aaaactttta aacattctct cttttacaaa aataaactta ttttgtactt taaaaacagt
     3301 catgttgtat tataaaataa gtaattagct taacttatac ataatagaaa caaattatac
     3361 ttattagtca gtcagaaaca actttggcac atatcaatat tatgctctcg acaaataact
     3421 tttttgcatt ttttgcacga tgcatttgcc tttcgcctta ttttagaggg gcagtaagta
     3481 cagtaagtac gttttttcat tactggctct tcagtactgt catctgatgt accaggcact
     3541 tcatttggca aaatattaga gatattatcg cgcaaatatc tcttcaaagt aggagcttct
     3601 aaacgcttac gcataaacga tgacgtcagg ctcatgtaaa ggtttctcat aaattttttg
     3661 cgactttgaa ccttttctcc cttgctactg acattatggc tgtatataat aaaagaattt
     3721 atgcaggcaa tgtttatcat tccgtacaat aatgccatag gccacctatt cgtcttccta
     3781 ctgcaggtca tcacagaaca catttggtct agcgtgtcca ctccgccttt agtttgatta
     3841 taatacataa ccatttgcgg tttaccggta ctttcgttga tagaagcatc ctcatcacaa
     3901 gatgataata agtataccat cttagctggc ttcggtttat atgagacgag agtaaggggt
     3961 ccgtcaaaac aaaacatcga tgttcccact ggcctggagc gactgttttt cagtacttcc
     4021 ggtatctcgc gtttgtttga tcgcacggtt cccacaatgg ttaattcgag ctcgcccggg
     4081 ctcctaggag atcttcatca caaacgaaac tattataatc gtcttcaggg gaacggtagc
     4141 atcatatctg tcaccagtca gtcggattcc tttctttcgg cataaattat gctcgggtga
     4201 cgagacgagt tgaaaatccc gtaaattaaa acatatacca cactcgccgt cctagacgac
     4261 gacgacgacg acgattccca aggaattgaa cccttcattt gcatttcact accgctctga
     4321 gagatgcaaa gcgcgccaga cagtcgatcg aatctgcaga ccagagagtc acatcaagaa
     4381 tgttgacatt ccaccagaag actctaatta ttcttcgctc gactgggaaa actgttaaca
     4441 actgctcgag tgtggtcgga gcggctcaca gtgcgttttc ccgtagacaa aaatcaaatt
     4501 tcatggtatt tttcggcgat aaacagtaag ttaaagggaa cccgaattta gtgctatata
     4561 atttaattcc actagagttt gtatcctttg atagatacgc gtatttcgac ctcaactgca
     4621 aggccgtcgt gtactagact tgactaatcc agactggtct tttagttatg acttctgtcc
     4681 acatctccat acattcaacg cactgtgcgg ctgtgctgtg cgactccgtc gagtcgacca
     4741 acatagttga aacaaattga atatttaatt gatcgttata ggaatggtgt tagatgagtc
     4801 atcctttaca gtaagcacat acagtattat aattgaagat cgtcggcaga taggtgtgta
     4861 gggtagagta tcagcaataa gttgggacgt ttgacttttt gtaggtagac aaaaactaaa
     4921 ctttttttcg cttctctatg tgtgcccctg ggtagcgttc cgttccgatt ggggtgcgaa
     4981 cgaatgaaat cgcccatcga gttgatacgt ccatccatcg ctagaaccgc gttcgctgta
     5041 gaagactata taagagcaga ggcaagagta gtgaaatgct agcaagtaaa cccctaccaa
     5101 ctggtcgggg tttgaaacgc agatgaactt cagggtcagc ttgccgtaca agtaaacccc
     5161 taccaactgg tcggggtttg aaactcatgt ggtcggggta gcggctgaag cactcaagta
     5221 aacccctacc aactggtcgg ggtttgaaac actccagctt gtgccccagg atgttgccgt
     5281 caagtaaacc cctaccaact ggtcggggtt tgaaactcac cttgatgccg ttcttctgct
     5341 tgtcggcaag taaaccccta ccaactggtc ggggtttgaa actttttttg gtggttgttc
     5401 atcgattcct aatattgcag tgaagcagtt tgttgtgcat ggcgaagata aggttagggc
     5461 gaggaatgcc ttggaaataa gcggcactgg gccgtatgaa taaaaagcgt tgaactttac
     5521 tacatgtagc atctactcaa aaacaaaatc cacaaagttt actacacttt tggtatgaat
     5581 aaaaaccttt tcgaacatgt agtacctact actttaagaa aattctattc aaagtacaga
     5641 gtaatgctgc atgttaagaa aattccattc agagtgactc ttgaaatcaa taaaatcatg
     5701 catatatgcc aaatttgtca cttattcacg tgacaaatta tgtgaaaata tgaatattac
     5761 tacattcccg ctatgcatcg cgtaatcgat catgaaattt atgttggtta tgtaacttta
     5821 cagtattccc tttatgtgca tgtgaatttt ataatgattt gacaacaagg attttcctta
     5881 gaattttcga attttagcta gaaatctgta tcctatgaat gaatcttgag ataacagcaa
     5941 ttgtaacatt ataaagttcc taacattttc aagattccag tattgaggcc ttttgggcca
     6001 tactcggtgg cctccccacc accaactttt ttgcactgca aaaaaacacg cttttgcacg
     6061 cgggcccata catagtacaa actctacgtt tcgtagacta ttttacataa atagtctaca
     6121 ccgttgtata cgctccaaat acactaccac acattgaacc tttttgcagt gcaaaaaagt
     6181 acgtgtcggc agtcacgtag gccggcctta tcgggtcgcg tcctgtcacg tacgaatcac
     6241 attatcggac cggacgagtg ttgtcttatc gtgacaggac gccagcttcc tgtgttgcta
     6301 accgcagccg gacgcaactc cttatcggaa caggacgcgc ctccatatca gccgcgcgtt
     6361 atctcatgcg cgtgaccgga cacgaggcgc ccgtcccgct tatcgcgcct ataaatacag
     6421 cccgcaacga tctggtaaac acagttgaac agcatctgtt acagcgacac aacatgagcc
     6481 ggtccaacaa cgccaacgcg cccacgccat ccaaccgccg ccgcaacctg tctctggtga
     6541 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     6601 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     6661 gcaagctgac cctgaagttc atctgcacca ccagcaagct gcccgtgccc tggcccaccc
     6721 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     6781 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     6841 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     6901 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     6961 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     7021 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     7081 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     7141 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     7201 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaaa
     7261 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     7321 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     7381 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     7441 caagtaaaac ctctacaaat gtggtatggc tgattatgat cagtcgaccg gcgcgccaat
     7501 tcgagctcgc ccggggatct aattcaatta gagactaatt caattagagc taattcaatt
     7561 aggatccaag cttatcgatt tcgaaccctc gaccgccgga gtataaatag aggcgcttcg
     7621 tctacggagc gacaattcaa ttcaaacaag caaagtgaac acgtcgctaa gcgaaagcta
     7681 agcaaataaa caagcgcagc tgaacaagct aaacaatcgg ggtaccgcta gagtcgacgg
     7741 tacgatccac cggtcgccac catggtgagc aagggcgagg aggtcatcaa agagttcatg
     7801 cgcttcaagg tgcgcatgga gggctccatg aacggccacg agttcgagat cgagggcgag
     7861 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc
     7921 cccctgccct tcgcctggga catcctgtcc ccccagttca tgtacggctc caaggcgtac
     7981 gtgaagcacc ccgccgacat ccccgattac aagaagctgt ccttccccga gggcttcaag
     8041 tgggagcgcg tgatgaactt cgaggacggc ggtctggtga ccgtgaccca ggactcctcc
     8101 ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt cccccccgac
     8161 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg cctgtacccc
     8221 cgcgacggcg tgctgaaggg cgagatccac caggccctga agctgaagga cggcggccac
     8281 tacctggtgg agttcaagac catctacatg gccaagaagc ccgtgcaact gcccggctac
     8341 tactacgtgg acaccaagct ggacatcacc tcccacaacg aggactacac catcgtggaa
     8401 cagtacgagc gctccgaggg ccgccaccac ctgttcctgg ggcatggcac cggcagcacc
     8461 ggcagcggca gctccggcac cgcctcctcc gaggacaaca acatggccgt tatcaaggaa
     8521 tttatgcgct tcaaagttag gatggaggga tccatgaacg gacatgagtt cgagatcgag
     8581 ggagagggcg agggacgccc gtatgaaggc acacaaacag ccaaactcaa ggtcaccaag
     8641 ggcggaccac tgcccttcgc ctgggatatc ctgagtcccc agtttatgta cggcagcaag
     8701 gcctacgtta agcaccccgc tgacataccg gactacaaaa agctgtcctt tccggaaggc
     8761 ttcaagtggg agcgcgtgat gaatttcgaa gacggaggac tggtcactgt gacccaagat
     8821 agcagtttgc aggacggtac actgatctat aaggttaaaa tgcgcggcac taactttccg
     8881 ccagatggcc cagtgatgca gaagaagacc atgggttggg aggcatccac cgaacgtctg
     8941 taccctcgag acggagtgct caagggcgag atccatcagg ccctcaaact gaaagatggt
     9001 ggtcactacc tggtcgaatt taagaccatt tacatggcca agaagccggt tcagctgccc
     9061 ggatattatt atgtggatac gaaactggat ataacttcgc ataacgaaga ctacaccatt
     9121 gtcgagcagt atgagcgcag cgaaggccga catcacctgt tcctctacgg catggacgag
     9181 ctgtacaagt aggcggccgc gactctagat cataatcagc cataccacat ttgtagaggt
     9241 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     9301 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     9361 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     9421 catcaatgta tcttaaagct tctaggccgg ccgatctcgg atctgacaat gttcagtgca
     9481 gagactcggc tacgcctcgt ggactttgaa gttgaccaac aatgtttatt cttacctcta
     9541 atagtcctct gtggcaaggt caagattctg ttagaagcca atgaagaacc tggttgttca
     9601 ataacatttt gttcgtctaa tatttcacta ccgcttgacg ttggctgcac ttcatgtacc
     9661 tcatctataa acgcttcttc tgtatcgctc tggacgtcat cttcacttac gtgatctgat
     9721 atttcactgt cagaatcctc accaacaagc tcgtcatcgc tttgcagaag agcagagagg
     9781 atatgctcat cgtctaaaga actacccatt ttattatata ttagtcacga tatctataac
     9841 aagaaaatat atatataata agttatcacg taagtagaac atgaaataac aatataatta
     9901 tcgtatgagt taaatcttaa aagtcacgta aaagataatc atgcgtcatt ttgactcacg
     9961 cggtcgttat agttcaaaat cagtgacact taccgcattg acaagcacgc ctcacgggag
    10021 ctccaagcgg cgactgagat gtcctaaatg cacagcgacg gattcgcgct atttagaaag
    10081 agagagcaat atttcaagaa tgcatgcgtc aattttacgc agactatctt tctagggtta
    10141 aaaaagattt gcgctttact cgacctaaac tttaaacacg tcatagaatc ttcgtttgac
    10201 aaaaaccaca ttgtggccaa gctgtgtgac gcgacgcgcg ctaaagaatg gcaaaccaag
    10261 tcgcgcgagc gtcgactcta gaggatcccc gggtaccgag ctcgaattcg taatcatgtc
    10321 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
    10381 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
    10441 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
    10501 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
    10561 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
    10621 acggt
//
