LOCUS       sequence-381990-       11148 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pBac-AedesUbiq-RfxCas13d-p10-Opie2-dsRed..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11148)
  AUTHORS   Dalla Benetta E, Lopez-Denman AJ, Li HH, Masri RA, Brogan DJ, Bui M,
            Yang T, Li M, Dunn M, Klein MJ, Jackson S, Catalan K, Blasdell KR, 
            Tng P, Antoshechkin I, Alphey LS, Paradkar PN, Akbari OS
  TITLE     Engineered Antiviral Sensor Targets Infected Mosquitoes.
  JOURNAL   CRISPR J. 2023 Dec;6(6):543-556. doi: 10.1089/crispr.2023.0056.
  PUBMED    38108518
REFERENCE   2  (bases 1 to 11148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/crispr.2023"; journalName: "CRISPR J"; date: "2023-12"; 
            volume: "6"; issue: "6"; pages: "543-556"
COMMENT     Sequence Label: OA-1050T
FEATURES             Location/Qualifiers
     source          1..11148
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(95..683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(175..194)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(854..1645,1646..1714))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1477..1496
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1715..1819)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1887..1905
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1943..1965)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2065..2084
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2278..2300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2292..2309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2293..2309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   3013..3075
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    4109..4178
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             4815..4835
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4845..7742
                     /codon_start=1
                     /product="RNA-targeting Type VI CRISPR protein from 
                     Ruminococcus flavefaciens strain XPD3002 (Konermann et al.,
                     2018)"
                     /label=RfxCas13d
                     /translation="IEKKKSFAKGMGVKSTLVSGSKVYMTTFAEGSDARLEKIVEGDSI
                     RSVNEGEAFSAEMADKNAGYKIGNAKFSHPKGYAVVANNPLYTGPVQQDMLGLKETLEK
                     RYFGESADGNDNICIQVIHNILDIEKILAEYITNAAYAVNNISGLDKDIIGFGKFSTVY
                     TYDEFKDPEHHRAAFNNNDKLINAIKAQYDEFDNFLDNPRLGYFGQAFFSKEGRNYIIN
                     YGNECYDILALLSGLRHWVVHNNEEESRISRTWLYNLDKNLDNEYISTLNYLYDRITNE
                     LTNSFSKNSAANVNYIAETLGINPAEFAEQYFRFSIMKEQKNLGFNITKLREVMLDRKD
                     MSEIRKNHKVFDSIRTKVYTMMDFVIYRYYIEEDAKVAAANKSLPDNEKSLSEKDIFVI
                     NLRGSFNDDQKDALYYDEANRIWRKLENIMHNIKEFRGNKTREYKKKDAPRLPRILPAG
                     RDVSAFSKLMYALTMFLDGKEINDLLTTLINKFDNIQSFLKVMPLIGVNAKFVEEYAFF
                     KDSAKIADELRLIKSFARMGEPIADARRAMYIDAIRILGTNLSYDELKALADTFSLDEN
                     GNKLKKGKHGMRNFIINNVISNKRFHYLIRYGDPAHLHEIAKNEAVVKFVLGRIADIQK
                     KQGQNGKNQIDRYYETCIGKDKGKSVSEKVDALTKIITGMNYDQFDKKRSVIEDTGREN
                     AEREKFKKIISLYLTVIYHILKNIVNINARYVIGFHCVERDAQLYKEKGYDINLKKLEE
                     KGFSSVTKLCAGIDETAPDKRKDVEKEMAERAKESIDSLESANPKLYANYIKYSDEKKA
                     EEFTRQINREKAKTALNAYLRNTKWNVIIREDLLRIDNKTCTLFRNKAVHLEVARYVHA
                     YINDIAEVNSYFQLYHYIMQRIIMNERYEKSSGKVSEYFDAVNDEKKYNDRLLKLLCVP
                     FGYCIPRFKNLSIEALFDRNEAAKFDKEKKKVSGNS"
     CDS             7752..7772
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7782..7808
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(9043..9045,9046..9048,9049..9723)
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     complement(9226..9246)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     9444..9463
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     9638..9661
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    9837..9958
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9874..9893)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9928..9947
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   10626..10660
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(10841..10857)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10841..10857)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10854..10876)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10865..10881
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10889..10895,10896..10913,10914..10919))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10934..10955
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11072..11089)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
       61 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      121 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      181 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      241 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
      301 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      361 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      421 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      481 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
      541 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      601 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      661 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
      721 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
      781 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
      841 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      901 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      961 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1021 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1081 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1141 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1201 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1261 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1321 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1381 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1441 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1501 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1561 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1621 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1681 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1741 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1801 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     1861 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     1921 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     1981 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     2041 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     2101 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     2161 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     2221 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     2281 agtcacgacg ttgtaaaacg acggccagtg ccaagctttg tttaaaaata taacaaaatt
     2341 gtgatcccac aaaatgaagt ggggcaaaat caaataatta atagtgtccg taaacttgtt
     2401 ggtcttcaac tttttgagga acacgttgga cggcaaatcc gtgactataa cacaagttga
     2461 tttaataatt ttagccaaca cgtcgggctg cgtgtttttt gccgacgcgt ctgtgtacac
     2521 gttgattaac tggtcgatta aactgttgaa ataatttaat ttttggttct tctttaaatc
     2581 tgtgatgaaa ttttttaaaa taactttaaa ttcttcattg gtaaaaaatg ccacgttttg
     2641 caacttgtga gggtctaata tgaggtcaaa ctcagtagga gttttatcca aaaaagaaaa
     2701 catgattacg tctgtacacg aacgcgtatt aacgcagagt gcaaagtata agagggttaa
     2761 aaaatatatt ttacgcacca tatacgcatc gggttgatat cgttaatatg gatcaatttg
     2821 aacagttgat taacgtgtct ctgctcaagt ctttgatcaa aacgcaaatc gacgaaaatg
     2881 tgtcggacaa tatcaagtcg atgagcgaaa aactaaaaag gctagaatac gacaatctca
     2941 cagacagcgt tgagatatac ggtattcacg acagcaggct gaataataaa aaaattagaa
     3001 actattattt aaccctagaa agataatcat attgtgacgt acgttaaaga taatcatgcg
     3061 taaaattgac gcatgtgttt tatcggtctg tatatcgagg tttatttatt aatttgaata
     3121 gatattaagt tttattatat ttacacttac atactaataa taaattcaac aaacaattta
     3181 tttatgttta tttatttatt aaaaaaaaac aaaaactcaa aatttcttct ataaagtaac
     3241 aaaactttta aacattctct cttttacaaa aataaactta ttttgtactt taaaaacagt
     3301 catgttgtat tataaaataa gtaattagct taacttatac ataatagaaa caaattatac
     3361 ttattagtca gtcagaaaca actttggcac atatcaatat tatgctctcg acaaataact
     3421 tttttgcatt ttttgcacga tgcatttgcc tttcgcctta ttttagaggg gcagtaagta
     3481 cagtaagtac gttttttcat tactggctct tcagtactgt catctgatgt accaggcact
     3541 tcatttggca aaatattaga gatattatcg cgcaaatatc tcttcaaagt aggagcttct
     3601 aaacgcttac gcataaacga tgacgtcagg ctcatgtaaa ggtttctcat aaattttttg
     3661 cgactttgaa ccttttctcc cttgctactg acattatggc tgtatataat aaaagaattt
     3721 atgcaggcaa tgtttatcat tccgtacaat aatgccatag gccacctatt cgtcttccta
     3781 ctgcaggtca tcacagaaca catttggtct agcgtgtcca ctccgccttt agtttgatta
     3841 taatacataa ccatttgcgg tttaccggta ctttcgttga tagaagcatc ctcatcacaa
     3901 gatgataata agtataccat cttagctggc ttcggtttat atgagacgag agtaaggggt
     3961 ccgtcaaaac aaaacatcga tgttcccact ggcctggagc gactgttttt cagtacttcc
     4021 ggtatctcgc gtttgtttga tcgcacggtt cccacaatgg ttaattcgag ctcgcccggg
     4081 gtcctaggtc gacgatgtag gtcacggtct cgaagccgcg gtgcgggtgc cagggcgtgc
     4141 ccttgggctc cccgggcgcg tactccacct cacccatctg gtccatcatg atgaacgggt
     4201 cgaggtggcg gtagttgatc ccggcgaacg cgcggcgcac cgggaagccc tcgccctcga
     4261 aaccgctggg cgcggtggtc acggtgagca cgggacgtgc gacggcgtcg gcgggtgcgg
     4321 atacgcgggg cagcgtcagc gggttctcga cggtcacggc gggcatgtcg acgcggccgc
     4381 agcgtgaacc ttgcatgcgt gcacagaatt tgattgcatt tcatgaccgg gtatccattc
     4441 ggcgccggcc actgaactga aattcagttt tgacagctcc actgacttga agctcttttt
     4501 caccgaaaca catctcggaa gagacacgtt gggaaagagt aagtttaatt tttgtcgttt
     4561 ttcgtggatt tttagtgata attcgtggtt tttcgttgtt agcaatctaa atcattgata
     4621 gatcaagtta gaaattgtga aatattgcta aaatacctac attattcgta gtttattcga
     4681 tttgttgtag ttgatgcatt ctaacctcaa tgtggtttcg tctccgtcca attccgatgt
     4741 tattgtccag ttcgttaatt tttggtgttt tctccccgac ttttccacag actgatccaa
     4801 gttaattaat gagccccaag aagaagagaa aggtggaggc cagcatcgaa aaaaaaaagt
     4861 ccttcgccaa gggcatgggc gtgaagtcca cactcgtgtc cggctccaaa gtgtacatga
     4921 caaccttcgc cgaaggcagc gacgccaggc tggaaaagat cgtggagggc gacagcatca
     4981 ggagcgtgaa tgagggcgag gccttcagcg ctgaaatggc cgataaaaac gccggctata
     5041 agatcggcaa cgccaaattc agccatccta agggctacgc cgtggtggct aacaaccctc
     5101 tgtatacagg acccgtccag caggatatgc tcggcctgaa ggaaactctg gaaaagaggt
     5161 acttcggcga gagcgctgat ggcaatgaca atatttgtat ccaggtgatc cataacatcc
     5221 tggacattga aaaaatcctc gccgaataca ttaccaacgc cgcctacgcc gtcaacaata
     5281 tctccggcct ggataaggac attattggat tcggcaagtt ctccacagtg tatacctacg
     5341 acgaattcaa agaccccgag caccataggg ccgctttcaa caataacgat aagctcatca
     5401 acgccatcaa ggcccagtat gacgagttcg acaacttcct cgataacccc agactcggct
     5461 atttcggcca ggcctttttc agcaaggagg gcagaaatta catcatcaat tacggcaacg
     5521 aatgctatga cattctggcc ctcctgagcg gactgaggca ctgggtggtc cataacaacg
     5581 aagaagagtc caggatctcc aggacctggc tctacaacct cgataagaac ctcgacaacg
     5641 aatacatctc caccctcaac tacctctacg acaggatcac caatgagctg accaactcct
     5701 tctccaagaa ctccgccgcc aacgtgaact atattgccga aactctggga atcaaccctg
     5761 ccgaattcgc cgaacaatat ttcagattca gcattatgaa agagcagaaa aacctcggat
     5821 tcaatatcac caagctcagg gaagtgatgc tggacaggaa ggatatgtcc gagatcagga
     5881 aaaatcataa ggtgttcgac tccatcagga ccaaggtcta caccatgatg gactttgtga
     5941 tttataggta ttacatcgaa gaggatgcca aggtggctgc cgccaataag tccctccccg
     6001 ataatgagaa gtccctgagc gagaaggata tctttgtgat taacctgagg ggctccttca
     6061 acgacgacca gaaggatgcc ctctactacg atgaagctaa tagaatttgg agaaagctcg
     6121 aaaatatcat gcacaacatc aaggaattta ggggaaacaa gacaagagag tataagaaga
     6181 aggacgcccc tagactgccc agaatcctgc ccgctggccg tgatgtttcc gccttcagca
     6241 aactcatgta tgccctgacc atgttcctgg atggcaagga gatcaacgac ctcctgacca
     6301 ccctgattaa taaattcgat aacatccaga gcttcctgaa ggtgatgcct ctcatcggag
     6361 tcaacgctaa gttcgtggag gaatacgcct ttttcaaaga ctccgccaag atcgccgatg
     6421 agctgaggct gatcaagtcc ttcgctagaa tgggagaacc tattgccgat gccaggaggg
     6481 ccatgtatat cgacgccatc cgtattttag gaaccaacct gtcctatgat gagctcaagg
     6541 ccctcgccga caccttttcc ctggacgaga acggaaacaa gctcaagaaa ggcaagcacg
     6601 gcatgagaaa tttcattatt aataacgtga tcagcaataa aaggttccac tacctgatca
     6661 gatacggtga tcctgcccac ctccatgaga tcgccaaaaa cgaggccgtg gtgaagttcg
     6721 tgctcggcag gatcgctgac atccagaaaa aacagggcca gaacggcaag aaccagatcg
     6781 acaggtacta cgaaacttgt atcggaaagg ataagggcaa gagcgtgagc gaaaaggtgg
     6841 acgctctcac aaagatcatc accggaatga actacgacca attcgacaag aaaaggagcg
     6901 tcattgagga caccggcagg gaaaacgccg agagggagaa gtttaaaaag atcatcagcc
     6961 tgtacctcac cgtgatctac cacatcctca agaatattgt caatatcaac gccaggtacg
     7021 tcatcggatt ccattgcgtc gagcgtgatg ctcaactgta caaggagaaa ggctacgaca
     7081 tcaatctcaa gaaactggaa gagaagggat tcagctccgt caccaagctc tgcgctggca
     7141 ttgatgaaac tgcccccgat aagagaaagg acgtggaaaa ggagatggct gaaagagcca
     7201 aggagagcat tgacagcctc gagagcgcca accccaagct gtatgccaat tacatcaaat
     7261 acagcgacga gaagaaagcc gaggagttca ccaggcagat taacagggag aaggccaaaa
     7321 ccgccctgaa cgcctacctg aggaacacca agtggaatgt gatcatcagg gaggacctcc
     7381 tgagaattga caacaagaca tgtaccctgt tcagaaacaa ggccgtccac ctggaagtgg
     7441 ccaggtatgt ccacgcctat atcaacgaca ttgccgaggt caattcctac ttccaactgt
     7501 accattacat catgcagaga attatcatga atgagaggta cgagaaaagc agcggaaagg
     7561 tgtccgagta cttcgacgct gtgaatgacg agaagaagta caacgatagg ctcctgaaac
     7621 tgctgtgtgt gcctttcggc tactgtatcc ccaggtttaa gaacctgagc atcgaggccc
     7681 tgttcgatag gaacgaggcc gccaagttcg acaaggagaa aaagaaggtg tccggcaatt
     7741 ccggatccgg acctaagaaa aagaggaagg tggcggccgc ttacccatac gatgttccag
     7801 attacgctta agctagctag aatgaatcgt ttttaaaata acaaatcaat tgttttataa
     7861 tattcgtacg attctttgat tatgtaataa aatgtgatca ttaggaagat tacgaaaaat
     7921 ataaaaaata tgagttctgt gtgtataaca aatgctgtaa acgccacaat tgtgtttgtt
     7981 gcaaataaac ccatgattat ttgattaaaa ttgttgtttt ctttgttcat agacaatagt
     8041 gtgttttgcc taaacgtgta ctgcataaac tccatgcgag tgtatagcga gctagtggct
     8101 aacgcttgcc ccaccaaagt agattcgtca aaatcctcaa tttcatcacc ctcctccaag
     8161 tttaacattt ggccgtcgga attaacttct aaagatgcca cataatctaa taaatgaaat
     8221 agagattcaa acgtggcgtc atcgtccgtt tcgaccattt ccgaaaagaa ctcgggcata
     8281 aactctatga tttctctgga cgtggtgttg tcgaaactct caaagtacgc agtcaggaac
     8341 gtgcgcgaca tgtcgtcggg aaactcgcgc ggaaacatgt tgttgtaacc gaacgggtcc
     8401 catagcgcca aaaccaaatc tgccagcgtc aatagaatga gcacgatgcc gacaatggag
     8461 ctggcttgga tagcgattcg agttaacggc cggggcgcgc ccatactcgg tggcctcccc
     8521 accaccaact tttttgcact gcaaaaaaac acgcttttgc acgcgggccc atacatagta
     8581 caaactctac gtttcgtaga ctattttaca taaatagtct acaccgttgt atacgctcca
     8641 aatacactac cacacattga acctttttgc agtgcaaaaa agtacgtgtc ggcagtcacg
     8701 taggccggcc ttatcgggtc gcgtcctgtc acgtacgaat cacattatcg gaccggacga
     8761 gtgttgtctt atcgtgacag gacgccagct tcctgtgttg ctaaccgcag ccggacgcaa
     8821 ctccttatcg gaacaggacg cgcctccata tcagccgcgc gttatctcat gcgcgtgacc
     8881 ggacacgagg cgcccgtccc gcttatcgcg cctataaata cagcccgcaa cgatctggta
     8941 aacacagttg aacagcatct gttacagcga cacaacatga gccggtccaa caacgccaac
     9001 gcgcccacgc catccaaccg ccgccgcaac ctgtctctgg tgatggtgcg ctcctccaag
     9061 aacgtcatca aggagttcat gcgcttcaag gtgcgcatgg agggcaccgt gaacggccac
     9121 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggccacaa caccgtgaag
     9181 ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc
     9241 cagtacggct ccaaggtgta cgtgaagcac cccgccgaca tccccgacta caagaagctg
     9301 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
     9361 accgtgaccc aggactcctc cctgcaggac ggctgcttca tctacaaggt gaagttcatc
     9421 ggcgtgaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
     9481 tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca caaggccctg
     9541 aagctgaagg acggcggcca ctacctggtg gagttcaagt ccatctacat ggccaagaag
     9601 cccgtgcagc tgcccggcta ctactacgtg gactccaagc tggacatcac ctcccacaac
     9661 gaggactaca ccatcgtgga gcagtacgag cgcaccgagg gccgccacca cctgttcctg
     9721 taggactcta gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     9781 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     9841 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     9901 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaaa
     9961 gcttctaggc cggccgatct cggatctgac aatgttcagt gcagagactc ggctacgcct
    10021 cgtggacttt gaagttgacc aacaatgttt attcttacct ctaatagtcc tctgtggcaa
    10081 ggtcaagatt ctgttagaag ccaatgaaga acctggttgt tcaataacat tttgttcgtc
    10141 taatatttca ctaccgcttg acgttggctg cacttcatgt acctcatcta taaacgcttc
    10201 ttctgtatcg ctctggacgt catcttcact tacgtgatct gatatttcac tgtcagaatc
    10261 ctcaccaaca agctcgtcat cgctttgcag aagagcagag aggatatgct catcgtctaa
    10321 agaactaccc attttattat atattagtca cgatatctat aacaagaaaa tatatatata
    10381 ataagttatc acgtaagtag aacatgaaat aacaatataa ttatcgtatg agttaaatct
    10441 taaaagtcac gtaaaagata atcatgcgtc attttgactc acgcggtcgt tatagttcaa
    10501 aatcagtgac acttaccgca ttgacaagca cgcctcacgg gagctccaag cggcgactga
    10561 gatgtcctaa atgcacagcg acggattcgc gctatttaga aagagagagc aatatttcaa
    10621 gaatgcatgc gtcaatttta cgcagactat ctttctaggg ttaaaaaaga tttgcgcttt
    10681 actcgaccta aactttaaac acgtcataga atcttcgttt gacaaaaacc acattgtggc
    10741 caagctgtgt gacgcgacgc gcgctaaaga atggcaaacc aagtcgcgcg agcgtcgact
    10801 ctagaggatc cccgggtacc gagctcgaat tcgtaatcat gtcatagctg tttcctgtgt
    10861 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
    10921 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
    10981 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
    11041 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
    11101 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggt
//
