LOCUS       sequence-381991-       11178 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pBac-AedesUbiq-RfxCas13d (Codon optimized)-p10-Opie2-dsRed..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11178)
  AUTHORS   Dalla Benetta E, Lopez-Denman AJ, Li HH, Masri RA, Brogan DJ, Bui M,
            Yang T, Li M, Dunn M, Klein MJ, Jackson S, Catalan K, Blasdell KR, 
            Tng P, Antoshechkin I, Alphey LS, Paradkar PN, Akbari OS
  TITLE     Engineered Antiviral Sensor Targets Infected Mosquitoes.
  JOURNAL   CRISPR J. 2023 Dec;6(6):543-556. doi: 10.1089/crispr.2023.0056.
  PUBMED    38108518
REFERENCE   2  (bases 1 to 11178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/crispr.2023"; journalName: "CRISPR J"; date: "2023-12"; 
            volume: "6"; issue: "6"; pages: "543-556"
COMMENT     Sequence Label: OA-1163A
FEATURES             Location/Qualifiers
     source          1..11178
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(95..683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(175..194)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(854..1645,1646..1714))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1477..1496
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1715..1819)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1887..1905
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1943..1965)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2065..2084
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2278..2300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2292..2309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2293..2309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   3013..3075
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    4109..4178
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             4809..7709
                     /codon_start=1
                     /product="RNA-targeting Type VI CRISPR protein from 
                     Ruminococcus flavefaciens strain XPD3002 (Konermann et al.,
                     2018)"
                     /label=RfxCas13d
                     /translation="MIEKKKSFAKGMGVKSTLVSGSKVYMTTFAEGSDARLEKIVEGDS
                     IRSVNEGEAFSAEMADKNAGYKIGNAKFSHPKGYAVVANNPLYTGPVQQDMLGLKETLE
                     KRYFGESADGNDNICIQVIHNILDIEKILAEYITNAAYAVNNISGLDKDIIGFGKFSTV
                     YTYDEFKDPEHHRAAFNNNDKLINAIKAQYDEFDNFLDNPRLGYFGQAFFSKEGRNYII
                     NYGNECYDILALLSGLRHWVVHNNEEESRISRTWLYNLDKNLDNEYISTLNYLYDRITN
                     ELTNSFSKNSAANVNYIAETLGINPAEFAEQYFRFSIMKEQKNLGFNITKLREVMLDRK
                     DMSEIRKNHKVFDSIRTKVYTMMDFVIYRYYIEEDAKVAAANKSLPDNEKSLSEKDIFV
                     INLRGSFNDDQKDALYYDEANRIWRKLENIMHNIKEFRGNKTREYKKKDAPRLPRILPA
                     GRDVSAFSKLMYALTMFLDGKEINDLLTTLINKFDNIQSFLKVMPLIGVNAKFVEEYAF
                     FKDSAKIADELRLIKSFARMGEPIADARRAMYIDAIRILGTNLSYDELKALADTFSLDE
                     NGNKLKKGKHGMRNFIINNVISNKRFHYLIRYGDPAHLHEIAKNEAVVKFVLGRIADIQ
                     KKQGQNGKNQIDRYYETCIGKDKGKSVSEKVDALTKIITGMNYDQFDKKRSVIEDTGRE
                     NAEREKFKKIISLYLTVIYHILKNIVNINARYVIGFHCVERDAQLYKEKGYDINLKKLE
                     EKGFSSVTKLCAGIDETAPDKRKDVEKEMAERAKESIDSLESANPKLYANYIKYSDEKK
                     AEEFTRQINREKAKTALNAYLRNTKWNVIIREDLLRIDNKTCTLFRNKAVHLEVARYVH
                     AYINDIAEVNSYFQLYHYIMQRIIMNERYEKSSGKVSEYFDAVNDEKKYNDRLLKLLCV
                     PFGYCIPRFKNLSIEALFDRNEAAKFDKEKKKVSGNS"
     CDS             7722..7748
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             7758..7838
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             join(9073..9075,9076..9078,9079..9753)
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     complement(9256..9276)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     9474..9493
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     9668..9691
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    9867..9988
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9904..9923)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9958..9977
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   10656..10690
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(10871..10887)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10871..10887)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10884..10906)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10895..10911
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10919..10925,10926..10943,10944..10949))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10964..10985
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11102..11119)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
       61 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      121 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      181 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      241 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
      301 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      361 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      421 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      481 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
      541 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      601 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      661 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
      721 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
      781 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
      841 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      901 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      961 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1021 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1081 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1141 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1201 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1261 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1321 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1381 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1441 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1501 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1561 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1621 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1681 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1741 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1801 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     1861 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     1921 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     1981 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     2041 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     2101 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     2161 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     2221 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     2281 agtcacgacg ttgtaaaacg acggccagtg ccaagctttg tttaaaaata taacaaaatt
     2341 gtgatcccac aaaatgaagt ggggcaaaat caaataatta atagtgtccg taaacttgtt
     2401 ggtcttcaac tttttgagga acacgttgga cggcaaatcc gtgactataa cacaagttga
     2461 tttaataatt ttagccaaca cgtcgggctg cgtgtttttt gccgacgcgt ctgtgtacac
     2521 gttgattaac tggtcgatta aactgttgaa ataatttaat ttttggttct tctttaaatc
     2581 tgtgatgaaa ttttttaaaa taactttaaa ttcttcattg gtaaaaaatg ccacgttttg
     2641 caacttgtga gggtctaata tgaggtcaaa ctcagtagga gttttatcca aaaaagaaaa
     2701 catgattacg tctgtacacg aacgcgtatt aacgcagagt gcaaagtata agagggttaa
     2761 aaaatatatt ttacgcacca tatacgcatc gggttgatat cgttaatatg gatcaatttg
     2821 aacagttgat taacgtgtct ctgctcaagt ctttgatcaa aacgcaaatc gacgaaaatg
     2881 tgtcggacaa tatcaagtcg atgagcgaaa aactaaaaag gctagaatac gacaatctca
     2941 cagacagcgt tgagatatac ggtattcacg acagcaggct gaataataaa aaaattagaa
     3001 actattattt aaccctagaa agataatcat attgtgacgt acgttaaaga taatcatgcg
     3061 taaaattgac gcatgtgttt tatcggtctg tatatcgagg tttatttatt aatttgaata
     3121 gatattaagt tttattatat ttacacttac atactaataa taaattcaac aaacaattta
     3181 tttatgttta tttatttatt aaaaaaaaac aaaaactcaa aatttcttct ataaagtaac
     3241 aaaactttta aacattctct cttttacaaa aataaactta ttttgtactt taaaaacagt
     3301 catgttgtat tataaaataa gtaattagct taacttatac ataatagaaa caaattatac
     3361 ttattagtca gtcagaaaca actttggcac atatcaatat tatgctctcg acaaataact
     3421 tttttgcatt ttttgcacga tgcatttgcc tttcgcctta ttttagaggg gcagtaagta
     3481 cagtaagtac gttttttcat tactggctct tcagtactgt catctgatgt accaggcact
     3541 tcatttggca aaatattaga gatattatcg cgcaaatatc tcttcaaagt aggagcttct
     3601 aaacgcttac gcataaacga tgacgtcagg ctcatgtaaa ggtttctcat aaattttttg
     3661 cgactttgaa ccttttctcc cttgctactg acattatggc tgtatataat aaaagaattt
     3721 atgcaggcaa tgtttatcat tccgtacaat aatgccatag gccacctatt cgtcttccta
     3781 ctgcaggtca tcacagaaca catttggtct agcgtgtcca ctccgccttt agtttgatta
     3841 taatacataa ccatttgcgg tttaccggta ctttcgttga tagaagcatc ctcatcacaa
     3901 gatgataata agtataccat cttagctggc ttcggtttat atgagacgag agtaaggggt
     3961 ccgtcaaaac aaaacatcga tgttcccact ggcctggagc gactgttttt cagtacttcc
     4021 ggtatctcgc gtttgtttga tcgcacggtt cccacaatgg ttaattcgag ctcgcccggg
     4081 gtcctaggtc gacgatgtag gtcacggtct cgaagccgcg gtgcgggtgc cagggcgtgc
     4141 ccttgggctc cccgggcgcg tactccacct cacccatctg gtccatcatg atgaacgggt
     4201 cgaggtggcg gtagttgatc ccggcgaacg cgcggcgcac cgggaagccc tcgccctcga
     4261 aaccgctggg cgcggtggtc acggtgagca cgggacgtgc gacggcgtcg gcgggtgcgg
     4321 atacgcgggg cagcgtcagc gggttctcga cggtcacggc gggcatgtcg acgcggccgc
     4381 agcgtgaacc ttgcatgcgt gcacagaatt tgattgcatt tcatgaccgg gtatccattc
     4441 ggcgccggcc actgaactga aattcagttt tgacagctcc actgacttga agctcttttt
     4501 caccgaaaca catctcggaa gagacacgtt gggaaagagt aagtttaatt tttgtcgttt
     4561 ttcgtggatt tttagtgata attcgtggtt tttcgttgtt agcaatctaa atcattgata
     4621 gatcaagtta gaaattgtga aatattgcta aaatacctac attattcgta gtttattcga
     4681 tttgttgtag ttgatgcatt ctaacctcaa tgtggtttcg tctccgtcca attccgatgt
     4741 tattgtccag ttcgttaatt tttggtgttt tctccccgac ttttccacag actgatccaa
     4801 gttaattaat gatcgaaaag aagaagtcgt tcgccaaggg aatgggagtg aagtcgaccc
     4861 tggtgtcggg atcgaaggtg tacatgacca ccttcgccga aggatcggat gcccgtctgg
     4921 aaaagatcgt ggaaggagat tcgatccgtt cggtgaacga aggagaagcc ttctcggccg
     4981 aaatggccga taagaacgcc ggatacaaga tcggaaacgc caagttctcg cacccgaagg
     5041 gatacgccgt ggtggccaac aacccgctgt acaccggacc ggtgcagcag gatatgctgg
     5101 gactgaagga aaccctggaa aagcgttact tcggagaatc ggccgatgga aacgataaca
     5161 tctgcatcca ggtgatccac aacatcctgg atatcgaaaa gatcctggcc gaatacatca
     5221 ccaacgccgc ctacgccgtg aacaacatct cgggactgga taaggatatc atcggattcg
     5281 gaaagttctc gaccgtgtac acctacgatg aattcaagga tccggaacac caccgtgccg
     5341 ccttcaacaa caacgataag ctgatcaacg ccatcaaggc ccagtacgat gaattcgata
     5401 acttcctgga taacccgcgt ctgggatact tcggacaggc cttcttctcg aaggaaggac
     5461 gtaactacat catcaactac ggaaacgaat gctacgatat cctggccctg ctgtcgggac
     5521 tgcgtcactg ggtggtgcac aacaacgaag aagaatcgcg tatctcgcgt acctggctgt
     5581 acaacctgga taagaacctg gataacgaat acatctcgac cctgaactac ctgtacgatc
     5641 gtatcaccaa cgaactgacc aactcgttct cgaagaactc ggccgccaac gtgaactaca
     5701 tcgccgaaac cctgggaatc aacccggccg aattcgccga acagtacttc cgtttctcga
     5761 tcatgaagga acagaagaac ctgggattca acatcaccaa gctgcgtgaa gtgatgctgg
     5821 atcgtaagga tatgtcggaa atccgtaaga accacaaggt gttcgattcg atccgtacca
     5881 aggtgtacac catgatggat ttcgtgatct accgttacta catcgaagaa gatgccaagg
     5941 tggccgccgc caacaagtcg ctgccggata acgaaaagtc gctgtcggaa aaggatatct
     6001 tcgtgatcaa cctgcgtgga tcgttcaacg atgatcagaa ggatgccctg tactacgatg
     6061 aagccaaccg tatctggcgt aagctggaaa acatcatgca caacatcaag gaattccgtg
     6121 gaaacaagac ccgtgaatac aagaagaagg atgccccgcg tctgccgcgt atcctgccgg
     6181 ccggacgtga tgtgtcggcc ttctcgaagc tgatgtacgc cctgaccatg ttcctggatg
     6241 gaaaggaaat caacgatctg ctgaccaccc tgatcaacaa gttcgataac atccagtcgt
     6301 tcctgaaggt gatgccgctg atcggagtga acgccaagtt cgtggaagaa tacgccttct
     6361 tcaaggattc ggccaagatc gccgatgaac tgcgtctgat caagtcgttc gcccgtatgg
     6421 gagaaccgat cgccgatgcc cgtcgtgcca tgtacatcga tgccatccgt atcctgggaa
     6481 ccaacctgtc gtacgatgaa ctgaaggccc tggccgatac cttctcgctg gatgaaaacg
     6541 gaaacaagct gaagaaggga aagcacggaa tgcgtaactt catcatcaac aacgtgatct
     6601 cgaacaagcg tttccactac ctgatccgtt acggagatcc ggcccacctg cacgaaatcg
     6661 ccaagaacga agccgtggtg aagttcgtgc tgggacgtat cgccgatatc cagaagaagc
     6721 agggacagaa cggaaagaac cagatcgatc gttactacga aacctgcatc ggaaaggata
     6781 agggaaagtc ggtgtcggaa aaggtggatg ccctgaccaa gatcatcacc ggaatgaact
     6841 acgatcagtt cgataagaag cgttcggtga tcgaagatac cggacgtgaa aacgccgaac
     6901 gtgaaaagtt caagaagatc atctcgctgt acctgaccgt gatctaccac atcctgaaga
     6961 acatcgtgaa catcaacgcc cgttacgtga tcggattcca ctgcgtggaa cgtgatgccc
     7021 agctgtacaa ggaaaaggga tacgatatca acctgaagaa gctggaagaa aagggattct
     7081 cgtcggtgac caagctgtgc gccggaatcg atgaaaccgc cccggataag cgtaaggatg
     7141 tggaaaagga aatggccgaa cgtgccaagg aatcgatcga ttcgctggaa tcggccaacc
     7201 cgaagctgta cgccaactac atcaagtact cggatgaaaa gaaggccgaa gaattcaccc
     7261 gtcagatcaa ccgtgaaaag gccaagaccg ccctgaacgc ctacctgcgt aacaccaagt
     7321 ggaacgtgat catccgtgaa gatctgctgc gtatcgataa caagacctgc accctgttcc
     7381 gtaacaaggc cgtgcacctg gaagtggccc gttacgtgca cgcctacatc aacgatatcg
     7441 ccgaagtgaa ctcgtacttc cagctgtacc actacatcat gcagcgtatc atcatgaacg
     7501 aacgttacga aaagtcgtcg ggaaaggtgt cggaatactt cgatgccgtg aacgatgaaa
     7561 agaagtacaa cgatcgtctg ctgaagctgc tgtgcgtgcc gttcggatac tgcatcccgc
     7621 gtttcaagaa cctgtcgatc gaagccctgt tcgatcgtaa cgaagccgcc aagttcgata
     7681 aggaaaagaa gaaggtgtcg ggaaactcgg gatcccttca actgcctcca cttgaaagac
     7741 tgacactggg tagttcctac ccatacgatg ttccagatta cgcttatccc tacgacgtgc
     7801 ctgattatgc atacccatat gatgtccccg actatgccta agctagctag aatgaatcgt
     7861 ttttaaaata acaaatcaat tgttttataa tattcgtacg attctttgat tatgtaataa
     7921 aatgtgatca ttaggaagat tacgaaaaat ataaaaaata tgagttctgt gtgtataaca
     7981 aatgctgtaa acgccacaat tgtgtttgtt gcaaataaac ccatgattat ttgattaaaa
     8041 ttgttgtttt ctttgttcat agacaatagt gtgttttgcc taaacgtgta ctgcataaac
     8101 tccatgcgag tgtatagcga gctagtggct aacgcttgcc ccaccaaagt agattcgtca
     8161 aaatcctcaa tttcatcacc ctcctccaag tttaacattt ggccgtcgga attaacttct
     8221 aaagatgcca cataatctaa taaatgaaat agagattcaa acgtggcgtc atcgtccgtt
     8281 tcgaccattt ccgaaaagaa ctcgggcata aactctatga tttctctgga cgtggtgttg
     8341 tcgaaactct caaagtacgc agtcaggaac gtgcgcgaca tgtcgtcggg aaactcgcgc
     8401 ggaaacatgt tgttgtaacc gaacgggtcc catagcgcca aaaccaaatc tgccagcgtc
     8461 aatagaatga gcacgatgcc gacaatggag ctggcttgga tagcgattcg agttaacggc
     8521 cggggcgcgc ccatactcgg tggcctcccc accaccaact tttttgcact gcaaaaaaac
     8581 acgcttttgc acgcgggccc atacatagta caaactctac gtttcgtaga ctattttaca
     8641 taaatagtct acaccgttgt atacgctcca aatacactac cacacattga acctttttgc
     8701 agtgcaaaaa agtacgtgtc ggcagtcacg taggccggcc ttatcgggtc gcgtcctgtc
     8761 acgtacgaat cacattatcg gaccggacga gtgttgtctt atcgtgacag gacgccagct
     8821 tcctgtgttg ctaaccgcag ccggacgcaa ctccttatcg gaacaggacg cgcctccata
     8881 tcagccgcgc gttatctcat gcgcgtgacc ggacacgagg cgcccgtccc gcttatcgcg
     8941 cctataaata cagcccgcaa cgatctggta aacacagttg aacagcatct gttacagcga
     9001 cacaacatga gccggtccaa caacgccaac gcgcccacgc catccaaccg ccgccgcaac
     9061 ctgtctctgg tgatggtgcg ctcctccaag aacgtcatca aggagttcat gcgcttcaag
     9121 gtgcgcatgg agggcaccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
     9181 cgcccctacg agggccacaa caccgtgaag ctgaaggtga ccaagggcgg ccccctgccc
     9241 ttcgcctggg acatcctgtc cccccagttc cagtacggct ccaaggtgta cgtgaagcac
     9301 cccgccgaca tccccgacta caagaagctg tccttccccg agggcttcaa gtgggagcgc
     9361 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
     9421 ggctgcttca tctacaaggt gaagttcatc ggcgtgaact tcccctccga cggccccgta
     9481 atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc
     9541 gtgctgaagg gcgagatcca caaggccctg aagctgaagg acggcggcca ctacctggtg
     9601 gagttcaagt ccatctacat ggccaagaag cccgtgcagc tgcccggcta ctactacgtg
     9661 gactccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga gcagtacgag
     9721 cgcaccgagg gccgccacca cctgttcctg taggactcta gatcataatc agccatacca
     9781 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     9841 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     9901 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     9961 gtttgtccaa actcatcaat gtatcttaaa gcttctaggc cggccgatct cggatctgac
    10021 aatgttcagt gcagagactc ggctacgcct cgtggacttt gaagttgacc aacaatgttt
    10081 attcttacct ctaatagtcc tctgtggcaa ggtcaagatt ctgttagaag ccaatgaaga
    10141 acctggttgt tcaataacat tttgttcgtc taatatttca ctaccgcttg acgttggctg
    10201 cacttcatgt acctcatcta taaacgcttc ttctgtatcg ctctggacgt catcttcact
    10261 tacgtgatct gatatttcac tgtcagaatc ctcaccaaca agctcgtcat cgctttgcag
    10321 aagagcagag aggatatgct catcgtctaa agaactaccc attttattat atattagtca
    10381 cgatatctat aacaagaaaa tatatatata ataagttatc acgtaagtag aacatgaaat
    10441 aacaatataa ttatcgtatg agttaaatct taaaagtcac gtaaaagata atcatgcgtc
    10501 attttgactc acgcggtcgt tatagttcaa aatcagtgac acttaccgca ttgacaagca
    10561 cgcctcacgg gagctccaag cggcgactga gatgtcctaa atgcacagcg acggattcgc
    10621 gctatttaga aagagagagc aatatttcaa gaatgcatgc gtcaatttta cgcagactat
    10681 ctttctaggg ttaaaaaaga tttgcgcttt actcgaccta aactttaaac acgtcataga
    10741 atcttcgttt gacaaaaacc acattgtggc caagctgtgt gacgcgacgc gcgctaaaga
    10801 atggcaaacc aagtcgcgcg agcgtcgact ctagaggatc cccgggtacc gagctcgaat
    10861 tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
    10921 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
    10981 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
    11041 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
    11101 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
    11161 caaaggcggt aatacggt
//
