LOCUS       sequence-382002-        5877 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for Camk2a expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Li H, Ou J, Li Y, Xu N, Li Q, Wu P, Peng C, Tang YC, Chang HC
  TITLE     ISX-9 potentiates CaMKIIdelta-mediated BMAL1 activation to enhance 
            circadian amplitude.
  JOURNAL   Commun Biol. 2022 Jul 28;5(1):750. doi: 10.1038/s42003-022-03725-x.
  PUBMED    35902736
REFERENCE   2  (bases 1 to 5877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-07-28"; volume: "5(1):750. doi"; pages: " 
            10.1038/s42003-022-03725-x"
COMMENT     Sequence Label: pGL3-Camk2a
FEATURES             Location/Qualifiers
     source          1..5877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1141..1150
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1147..2799
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1213..1231)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    2840..2961
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2851..2870)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2905..2924
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3209..3226)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3380..3968)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3460..3479)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4139..4930,4931..4999))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4762..4781
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5000..5104)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5131..5586
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5218..5237)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5428..5449
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    5717..5765
                     /note="synthetic polyadenylation signal"
     misc_feature    5779..5870
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5819..5838
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggtaccgagc tcttacgcgt tacatctaca ccacccgccc agccctgggt gagtccagcc
       61 acgttcacct cattatagtt gcctctctcc agtcctacct tgacgggaag cacaagcaga
      121 aactgggaca ggagccccag gagaccaaat cttcatggtc cctctgggag gatgggtggg
      181 gagagctgtg gcagaggcct caggaggggc cctgctgctc agtggtgaca gataggggtg
      241 agaaagcaga cagagtcatt ccgtcagcat tctgggtctg tttggtactt cttctcacga
      301 taaggtggcg gtgtgatatg cacaatggct aaaaagcagg gagagctgga aagaaacaag
      361 gacagagaca gaggccaagt caaccagacc aattcccaga ggaagcaaag aaaccattac
      421 agagactaca agggggaagg gaaggagaga tgaattagct tcccctgtaa accttagaac
      481 ccagctgttg ccagggcaac ggggcaatac ctgtctcttc agaggagatg aagttgccag
      541 ggtaactaca tcctgtcttt ctcaaggacc atcccagaat gtggcaccca ctagccgtta
      601 ccatagcaac tgcctctttg ccccacttaa tcccatcccg tctgttaaaa gggccctata
      661 gttggaggtg ggggaggtag gaagagcgat gatcacttgt ggactaagtt tgttcgcatc
      721 cccttctcca accccctcag tacatcaccc tgggggaaca gggtccactt gctcctgggc
      781 ccacacagtc ctgcagtatt gtgtatataa ggccagggca aagaggagca ggttttaaag
      841 tgaaaggcag gcaggtgttg gggaggcagt taccggggca acgggaacag ggcgtttcgg
      901 aggtggttgc catggggacc tggatgctga cgaaggctcg cgaggctgtg agcagccaca
      961 gtgccctgct cagaagcccc aagctcgtca gtcaagccgg ttctccgttt gcactcagga
     1021 gcacgggcag gcgagtggcc cctagttctg ggggcagcgc ttcagcatcc cagccctagt
     1081 tcccagccta aagcagatct gcgatctaag taagcttggc attccggtac tgttggtaaa
     1141 gccaccatgg aagacgccaa aaacataaag aaaggcccgg cgccattcta tccgctggaa
     1201 gatggaaccg ctggagagca actgcataag gctatgaaga gatacgccct ggttcctgga
     1261 acaattgctt ttacagatgc acatatcgag gtggacatca cttacgctga gtacttcgaa
     1321 atgtccgttc ggttggcaga agctatgaaa cgatatgggc tgaatacaaa tcacagaatc
     1381 gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc gttatttatc
     1441 ggagttgcag ttgcgcccgc gaacgacatt tataatgaac gtgaattgct caacagtatg
     1501 ggcatttcgc agcctaccgt ggtgttcgtt tccaaaaagg ggttgcaaaa aattttgaac
     1561 gtgcaaaaaa agctcccaat catccaaaaa attattatca tggattctaa aacggattac
     1621 cagggatttc agtcgatgta cacgttcgtc acatctcatc tacctcccgg ttttaatgaa
     1681 tacgattttg tgccagagtc cttcgatagg gacaagacaa ttgcactgat catgaactcc
     1741 tctggatcta ctggtctgcc taaaggtgtc gctctgcctc atagaactgc ctgcgtgaga
     1801 ttctcgcatg ccagagatcc tatttttggc aatcaaatca ttccggatac tgcgatttta
     1861 agtgttgttc cattccatca cggttttgga atgtttacta cactcggata tttgatatgt
     1921 ggatttcgag tcgtcttaat gtatagattt gaagaagagc tgtttctgag gagccttcag
     1981 gattacaaga ttcaaagtgc gctgctggtg ccaaccctat tctccttctt cgccaaaagc
     2041 actctgattg acaaatacga tttatctaat ttacacgaaa ttgcttctgg tggcgctccc
     2101 ctctctaagg aagtcgggga agcggttgcc aagaggttcc atctgccagg tatcaggcaa
     2161 ggatatgggc tcactgagac tacatcagct attctgatta cacccgaggg ggatgataaa
     2221 ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga aggttgtgga tctggatacc
     2281 gggaaaacgc tgggcgttaa tcaaagaggc gaactgtgtg tgagaggtcc tatgattatg
     2341 tccggttatg taaacaatcc ggaagcgacc aacgccttga ttgacaagga tggatggcta
     2401 cattctggag acatagctta ctgggacgaa gacgaacact tcttcatcgt tgaccgcctg
     2461 aagtctctga ttaagtacaa aggctatcag gtggctcccg ctgaattgga atccatcttg
     2521 ctccaacacc ccaacatctt cgacgcaggt gtcgcaggtc ttcccgacga tgacgccggt
     2581 gaacttcccg ccgccgttgt tgttttggag cacggaaaga cgatgacgga aaaagagatc
     2641 gtggattacg tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg agttgtgttt
     2701 gtggacgaag taccgaaagg tcttaccgga aaactcgacg caagaaaaat cagagagatc
     2761 ctcataaagg ccaagaaggg cggaaagatc gccgtgtaat tctagagtcg gggcggccgg
     2821 ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     2881 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     2941 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     3001 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat
     3061 aaggatccgt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc
     3121 gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg
     3181 acaggtgccg gcagcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     3241 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     3301 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     3361 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     3421 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     3481 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     3541 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     3601 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     3661 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     3721 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     3781 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     3841 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     3901 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     3961 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     4021 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     4081 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     4141 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     4201 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     4261 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     4321 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     4381 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     4441 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     4501 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     4561 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     4621 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     4681 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     4741 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     4801 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     4861 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     4921 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     4981 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     5041 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     5101 cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg
     5161 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     5221 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     5281 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     5341 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     5401 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     5461 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     5521 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     5581 caatttgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     5641 cttcgctatt acgccagccc aagctaccat gataagtaag taatattaag gtacgggagg
     5701 tacttggagc ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt
     5761 gtgtgaatcg atagtactaa catacgctct ccatcaaaac aaaacgaaac aaaacaaact
     5821 agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga acatttctct atcgata
//
