LOCUS       sequence-38205-e         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of mouse beta-catenin S33Y mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Takahashi K, Yamanaka S
  TITLE     Induction of pluripotent stem cells from mouse embryonic and adult 
            fibroblast cultures by defined factors.
  JOURNAL   Cell. 2006 Aug 25. 126(4):663-76.
  PUBMED    16904174
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2006 
            Aug 25. 126(4):663-76."
COMMENT     Sequence Label: pMXs-beta-catenin-S33Y
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    63..87
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             153..188
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
ORIGIN
        1 tcctctagac tgccggatct agctagttaa ttaaggatcc cagtgtggtg gtacgggaaa
       61 tcacaagttt gtacaaaaaa gcaggctccg cggccgcccc cttcaccatg gctactcaag
      121 ctgacctgat ggagttggac atggccatgg agccggacag aaaagctgct gtcagccact
      181 ggcagcagca gtcttactta gattatggaa tccattctgg tgccaccacc acagctcctt
      241 ccctgagtgg caagggcaac cctgaggaag aagatgttga cacctcccaa gtcctttatg
      301 aatgggagca aggcttttcc cagtccttca cgcaagagca agtagctgat attgacgggc
      361 agtatgcaat gactagggct cagagggtcc gagctgccat gttccctgag acgctagatg
      421 agggcatgca gatcccatcc acgcagtttg acgctgctca tcccactaat gtccagcgct
      481 tggctgaacc atcacagatg ttgaaacatg cagttgtcaa tttgattaac tatcaggatg
      541 acgcggaact tgccacacgt gcaattcctg agctgacaaa actgctaaac gatgaggacc
      601 aggtggtagt taataaagct gctgttatgg tccatcagct ttccaaaaag gaagcttcca
      661 gacatgccat catgcgctcc cctcagatgg tgtctgccat tgtacgcacc atgcagaata
      721 caaatgatgt agagacagct cgttgtactg ctgggactct gcacaacctt tctcaccacc
      781 gcgagggctt gctggccatc tttaagtctg gtggcatccc agcgctggtg aaaatgcttg
      841 ggtcaccagt ggattctgta ctgttctacg cc
//
