LOCUS       sequence-382073-        1845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for GST-based purification of 
            Drosophila melanogaster kinesin heavy chain [1-421] with N-terminal 
            SNAP tag and cleavable N-terminal GST.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1845)
  TITLE     Bacterial expression plasmids for the purification of labelled DmKHC
            for motor-PAINT
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGEX-6p-1_GST-SNAP-DmKHC[1-421]
FEATURES             Location/Qualifiers
     source          1..1845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..543
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="DKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVEV
                     PAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKV
                     VKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLAV
                     KEWLLAHEGHRLGKPGLG"
     CDS             556..576
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
ORIGIN
        1 gacaaagact gcgaaatgaa gcgcaccacc ctggatagcc ctctgggcaa gctggaactg
       61 tctgggtgcg aacagggcct gcaccgtatc atcttcctgg gcaaaggaac atctgccgcc
      121 gacgccgtgg aagtgcctgc cccagccgcc gtgctgggcg gaccagagcc actgatgcag
      181 gccaccgcct ggctcaacgc ctactttcac cagcctgagg ccatcgagga gttccctgtg
      241 ccagccctgc accacccagt gttccagcag gagagcttta cccgccaggt gctgtggaaa
      301 ctgctgaaag tggtgaagtt cggagaggtc atcagctaca gccacctggc cgccctggcc
      361 ggcaatcccg ccgccaccgc cgccgtgaaa accgccctga gcggaaatcc cgtgcccatt
      421 ctgatcccct gccaccgggt ggtgcagggc gacctggacg tggggggcta cgagggcggg
      481 ctcgccgtga aagagtggct gctggcccac gagggccaca gactgggcaa gcctgggctg
      541 ggtgagccaa ccactgagga tctgtacttt cagagcgata acagcgcaga acgagaaatt
      601 ccagccgagg acagcatcaa agtggtctgc cgattccgac cgctgaacga cagcgaagag
      661 aaggccggct ccaagttcgt ggtcaagttc cccaacaatg tggaggagaa ctgcatatcc
      721 atagcgggca aggtgtattt gttcgacaag gtcttcaaac cgaatgcatc ccaggaaaag
      781 gtctacaatg aggcggccaa gtccattgtt acggatgtcc tggccgggta caatggaacg
      841 atattcgcat acggtcagac gtcctccgga aaaacgcata cgatggaggg cgtgatcggg
      901 gactccgtaa aacagggtat catacctagg atcgtcaacg acattttcaa tcacatctac
      961 gcgatggagg tgaacctgga gtttcacatc aaggtctcct actacgagat ctacatggac
     1021 aagattcgag atctgttgga cgtctccaag gtgaacctta gtgtgcacga ggataagaac
     1081 cgggtgccgt acgtcaaggg cgctacggaa cggttcgtct cgtcgccgga ggatgttttc
     1141 gaggtgatcg aggagggcaa atccaatcgt cacatcgctg tgacaaacat gaacgagcat
     1201 tcttcgcgat cccactcagt attccttatc aatgtgaagc aggagaacct ggagaaccag
     1261 aagaaactat ccggcaaact ctacctggtg gatttggccg gatccgagaa ggtttccaag
     1321 actggagcgg agggaaccgt tcttgatgaa gccaagaaca tcaacaagtc gctgtcggcc
     1381 ttgggcaacg taatttctgc cctggcggac ggaaacaaaa cgcacatccc ctaccgtgat
     1441 tccaagctaa cgcgcatcct gcaggagtcg ctgggaggca acgcacgcac aaccatcgtc
     1501 atctgctgct ctccagccag tttcaacgaa tctgaaacga agtcaacgct ggacttcggt
     1561 cgtagagcca agacagtgaa gaacgtggtc tgcgttaacg aggagcttac tgccgaggaa
     1621 tggaagcgac gctatgaaaa ggagaaggaa aagaacgccc gactaaaggg taaggtggag
     1681 aagcttgaga tcgagcttgc gcgctggaga gcgggtgaaa ctgttaaggc ggaggagcaa
     1741 atcaacatgg aggatctcat ggaggcaagc acgcccaacc tggaagtgga ggcagcacag
     1801 acggcggcgg ccgaggccgc tttggccgcc cagcgaacgg cttag
//
