LOCUS       sequence-382074-        1839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for His tag-based purification of 
            Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal 
            SNAP tag and C-terminal 6xHis tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Deguchi T, Iwanski MK, Schentarra EM, Heidebrecht C, Schmidt L, Heck
            J, Weihs T, Schnorrenberg S, Hoess P, Liu S, Chevyreva V, Noh KM, 
            Kapitein LC, Ries J
  TITLE     Direct observation of motor protein stepping in living cells using 
            MINFLUX.
  JOURNAL   Science. 2023 Mar 10;379(6636):1010-1015. doi: 
            10.1126/science.ade2676. Epub 2023 Mar 9.
  PUBMED    36893247
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.ade2676"; journalName: "Science"; date: 
            "2023-03-10- 10"; volume: "379"; issue: "6636"; pages: "1010-1015"
COMMENT     Sequence Label: pET28a_DmKHC[1-421]-SNAP-6xHis
FEATURES             Location/Qualifiers
     source          1..1839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1270..1812
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="DKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVEV
                     PAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKV
                     VKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLAV
                     KEWLLAHEGHRLGKPGLG"
     CDS             1819..1836
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgagcgcag aacgagaaat tccagccgag gacagcatca aagtggtctg ccgattccga
       61 ccgctgaacg acagcgaaga gaaggccggc tccaagttcg tggtcaagtt ccccaacaat
      121 gtggaggaga actgcatatc catagcgggc aaggtgtatt tgttcgacaa ggtcttcaaa
      181 ccgaatgcat cccaggaaaa ggtctacaat gaggcggcca agtccattgt tacggatgtc
      241 ctggccgggt acaatggaac gatattcgca tacggtcaga cgtcctccgg aaaaacgcat
      301 acgatggagg gcgtgatcgg ggactccgta aaacagggta tcatacctag gatcgtcaac
      361 gacattttca atcacatcta cgcgatggag gtgaacctgg agtttcacat caaggtctcc
      421 tactacgaga tctacatgga caagattcga gatctgttgg acgtctccaa ggtgaacctt
      481 agtgtgcacg aggataagaa ccgggtgccg tacgtcaagg gcgctacgga acggttcgtc
      541 tcgtcgccgg aggatgtttt cgaggtgatc gaggagggca aatccaatcg tcacatcgct
      601 gtgacaaaca tgaacgagca ttcttcgcga tcccactcag tattccttat caatgtgaag
      661 caggagaacc tggagaacca gaagaaacta tccggcaaac tctacctggt ggatttggcc
      721 ggatccgaga aggtttccaa gactggagcg gagggaaccg ttcttgatga agccaagaac
      781 atcaacaagt cgctgtcggc cttgggcaac gtaatttctg ccctggcgga cggaaacaaa
      841 acgcacatcc cctaccgtga ttccaagcta acgcgcatcc tgcaggagtc gctgggaggc
      901 aacgcacgca caaccatcgt catctgctgc tctccagcca gtttcaacga atctgaaacg
      961 aagtcaacgc tggacttcgg tcgtagagcc aagacagtga agaacgtggt ctgcgttaac
     1021 gaggagctta ctgccgagga atggaagcga cgctatgaaa aggagaagga aaagaacgcc
     1081 cgactaaagg gtaaggtgga gaagcttgag atcgagcttg cgcgctggag agcgggtgaa
     1141 actgttaagg cggaggagca aatcaacatg gaggatctca tggaggcaag cacgcccaac
     1201 ctggaagtgg aggcagcaca gacggcggcg gccgaggccg ctttggccgc ccagcgaacg
     1261 gctgaattcg acaaagactg cgaaatgaag cgcaccaccc tggatagccc tctgggcaag
     1321 ctggaactgt ctgggtgcga acagggcctg caccgtatca tcttcctggg caaaggaaca
     1381 tctgccgccg acgccgtgga agtgcctgcc ccagccgccg tgctgggcgg accagagcca
     1441 ctgatgcagg ccaccgcctg gctcaacgcc tactttcacc agcctgaggc catcgaggag
     1501 ttccctgtgc cagccctgca ccacccagtg ttccagcagg agagctttac ccgccaggtg
     1561 ctgtggaaac tgctgaaagt ggtgaagttc ggagaggtca tcagctacag ccacctggcc
     1621 gccctggccg gcaatcccgc cgccaccgcc gccgtgaaaa ccgccctgag cggaaatccc
     1681 gtgcccattc tgatcccctg ccaccgggtg gtgcagggcg acctggacgt ggggggctac
     1741 gagggcgggc tcgccgtgaa agagtggctg ctggcccacg agggccacag actgggcaag
     1801 cctgggctgg gtctcgagca ccaccaccac caccactga
//
